Team:NYMU-Taipei/project/1c

From 2014.igem.org

(Difference between revisions)
Line 27: Line 27:
       <p>When the amount of the <i>S. mutans</i> is so large that the phages couldn't afford the loading, CSP would pass the threshold value and turn on the <b>luxR-luxI system</b>. LuxI acts as a gene which would generate AHL-synthase that yielding the quorum-sensing signal AHL right after the threshold level of <i>S. mutans</i> is exceeded. LuxR is a protein which forms a complex with AHL, and then turns into an activated form which will afterwards bind with the promoter pLuxR and trigger it.
       <p>When the amount of the <i>S. mutans</i> is so large that the phages couldn't afford the loading, CSP would pass the threshold value and turn on the <b>luxR-luxI system</b>. LuxI acts as a gene which would generate AHL-synthase that yielding the quorum-sensing signal AHL right after the threshold level of <i>S. mutans</i> is exceeded. LuxR is a protein which forms a complex with AHL, and then turns into an activated form which will afterwards bind with the promoter pLuxR and trigger it.
The signal sequence and endolysin downstream would be secreted and eliminate the excess <i>S. mutans</i>.</p>
The signal sequence and endolysin downstream would be secreted and eliminate the excess <i>S. mutans</i>.</p>
 +
      <p>Endolysin is an enzyme expressed by phage-infected bacteria. The C-terminus bind to the host cell wall, while the N-terminus is enzymatic domain. Working with holin, which lyses cell membrane, endolysin could break the cell wall of bacteria. Usually serve as peptidase, endolysin tend to work on gram positive species, which have layers of peptidoglycan cell wall. Though originally intended to be utilized inside the cell wall, endolysin can also be applied outside cell according to previous research. Furthermore, it is even less harmful to gram negative bacteria this way because of the lipid layer of those bacteria composes cell wall.</p>
 +
      <img src='/wiki/images/2/2c/NYMU14_target_cellwall.png'><p>Figure 1. Gram positive bacteria have only one layer of cell wall outside of cell membrane, composing of peptidoglycan, while the cell wall of gram negative bacteria has additional protection outside peptidoglycan cell wall.[1]</p>
 +
      <p></p>
       <h1 id='1c-2'>So how did we do it?</h1>
       <h1 id='1c-2'>So how did we do it?</h1>
      
      

Revision as of 15:32, 14 October 2014

X
Next ⇒
⇐ Prev
...
Control-Target

Here’s the Gist…

  • We incorporate our circuit into Streptococcus phage M102. The phage-infected Streptococcus mutans emit quorum-sensing signal will pass the message down to our probiotics terminus then trigger the S. mutans killing module of it.

Get started.

How to do it?

Test it!

Our result~

Reference

Before we get started:

The "control part" is going to control the number of S. mutans. When the number of S. mutans exceeds the threshold that causes cavities, the circuit will be activated, thus killing the excess S. mutans.

In order to find and confirm the threshold that causes cavities, we designed different kinds and numbers of terminators (each with different leakage rates) to create the threshold. Moreover, we combined both wet lab results and modelling to decide which design is more suitable.

Over the years, it has been found that the competence stimulating peptide, CSP, a quorum sensing chemical, is released in every competence S. mutans. Thus, we can detect the number of S. mutans by marking the amount of CSP.

In S. mutans the CSP bind to the membrane receptor, "comD", thereby phosphorylating the response regulator, "comE". The phosphorylated comE will activate numerous vital biological mechanisms in Streptococcus mutans such as biofilm formation and the release of mutacin, an antibiotic peptide. Compared with all the other mechanisms involved with CSP, it is found that the promoter of gene "nlmC"( non-lantibiotic mutacin C) in S. mutans has the best performance under the stimulation of CSP.

At this point, the two main organisms we adapted for our project are our probiotics and M102 bacteriophage. We used one of the most commonly seen quorum sensing chemicals, AHL, N-Acyl-homoserine-lactone, as our indicator between phage and E.coli. Due to the idea of phage threshold control of the number of S. mutans in prevention of the second-dominant strain in the oral cavity, we envision a possibility to trigger another killing system to reach synergistic effect on diminishing S. mutans. If the threshold terminators were passed, the downstream signaling pathway would be switched on and contact with the engineered probiotics.

When the amount of the S. mutans is so large that the phages couldn't afford the loading, CSP would pass the threshold value and turn on the luxR-luxI system. LuxI acts as a gene which would generate AHL-synthase that yielding the quorum-sensing signal AHL right after the threshold level of S. mutans is exceeded. LuxR is a protein which forms a complex with AHL, and then turns into an activated form which will afterwards bind with the promoter pLuxR and trigger it. The signal sequence and endolysin downstream would be secreted and eliminate the excess S. mutans.

Endolysin is an enzyme expressed by phage-infected bacteria. The C-terminus bind to the host cell wall, while the N-terminus is enzymatic domain. Working with holin, which lyses cell membrane, endolysin could break the cell wall of bacteria. Usually serve as peptidase, endolysin tend to work on gram positive species, which have layers of peptidoglycan cell wall. Though originally intended to be utilized inside the cell wall, endolysin can also be applied outside cell according to previous research. Furthermore, it is even less harmful to gram negative bacteria this way because of the lipid layer of those bacteria composes cell wall.

Figure 1. Gram positive bacteria have only one layer of cell wall outside of cell membrane, composing of peptidoglycan, while the cell wall of gram negative bacteria has additional protection outside peptidoglycan cell wall.[1]

So how did we do it?

Putting it to the test!

Our result

Reference

  1. http://www.expertsmind.com/topic/microbiology/bacterial-cell-wall-92313.aspx