Team:NYMU-Taipei/notebook/labnotes

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       <h>Lab notes</h>
       <h>Lab notes</h>
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      <div id='tabframe'>
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<img src='/wiki/images/7/7b/NYMU14_fNotebookSmall.png'>
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        <div class='note-tab' id='nt1'>introduction</div>
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        <div class='note-tab' id='nt2'>control</div>
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        <div class='note-tab' id='nt3'>communication</div>
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        <div class='note-tab' id='nt4'>cohesion</div>
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        <div class='note-tab' id='nt5'>completion</div>
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        <div class='note-tab' id='nt6'>function test</div>
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         <div class='note-tab'><p>labnote 1</p></div>
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         <div class='note-tab'><p>labnote 2</p></div>
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<h1>introduction :P introduction :P introduction :P introduction :P </h1>
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         <div class='note-tab'><p>labnote 3</p></div>
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<p>introduction of labnote</p>
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         <div class='note-tab'><p>labnote 4</p></div>
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<p>樹狀圖 timeline 順序圖</p>
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<p>under construction</p>
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         <div class='note-content' id='nt2-cont' style='display:none;'>
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<h1>lab note of circuit in the control part</h1>
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<p>part/gene transformation and test</p>
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<p>circuit contruction</p>
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<p>part function test</p>
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<h2>experiment1 date: 0707~0712</h2>
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<h2>experiment1 date: 0707~0712</h2>
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         <div class='note-content' id='nt3-cont' style='display:none;'>
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<h1>lab note of circuit in the communication part</h1>
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<p>part/gene transformation and test</p>
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<p>circuit contruction</p>
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<p>part function test</p>
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<h2>experiment1 date: 0707~0712</h2>
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<h2>experiment1 date: 0707~0712</h2>
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        <div class='note-content' id='nt4-cont' style='display:none;'>
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<h1>lab note of circuit in the cohesion part</h1>
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<p>part/gene transformation and test</p>
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<p>circuit contruction</p>
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<p>part function test</p>
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<p>under construction</p>
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<h2>experiment1 date: 0707~0712</h2>
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<h2>experiment1 date: 0707~0712</h2>
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<p>under construction</p>
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<p>under construction</p>
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<h2>experiment1 date: 0707~0712</h2>
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        <div class='note-content' id='nt5-cont' style='display:none;'>
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<h1>lab note of circuit in the completion part</h1>
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<h2>Parts from iGEM took kit</h2>
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<h3>Out line</h3>
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<p>For each part to be used in testing circuit:<br>BBa_J23100 , BBa_E1010, BBa_B0015
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</p>
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<img src=''><p>circuit 圖</p>
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<p>After transforming these plasmids into our competent cell, DH5a, we would take 3 steps to examine if we got the right product:</p>
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<ol>
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<li>Colony PCR and electrophoresis: Directly picking up colonies on designated antibiotic plate and do colony PCR with iGEM provided primers (VF2&VR), and then do electrophoresis to check the length of plasmid to confirm if the kit had been correctly transformed into E. coli.</li>
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<li>Plasmid PCR and electrophoresis: Colony PCR may not end up copying the right place, for there is extra DNA in the cell that is not the one of our interest. Therefore, we also check the length of the plasmid we which we transformed.</li>
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<li>Digestion and electrophoresis: After checking the length, we use EcoR1/Spe1 and Xba1/Pst1, and electrophoresis, to see if our constructed plasmid can be successfully digested.</li>
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</ol>
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<h2>experiment1 date: 0707~0712</h2>
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<p>under construction</p>
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         <div class='note-content' id='nt6-cont' style='display:none;'>
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<h1>lab note of function test in phage</h1>
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<p>part/gene transformation and test</p>
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<p>circuit contruction</p>
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<p>part function test</p>
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<p>under construction</p>
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<h2>experiment1 date: 0707~0712</h2>
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<p>under construction</p>
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<h2>experiment1 date: 0707~0712</h2>
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        </div>
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       </div>
       </div>
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      <embed src='/wiki/images/8/87/NYMU14_Labnote_Target1.pdf' class='note-pdf' style='display:block;'>
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      <embed src='/wiki/images/e/eb/NYMU14_Labnote_Target3.pdf' class='note-pdf' style='display:none;'>
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      <embed src='/wiki/images/9/93/NYMU14_labnote_inhibitor1.pdf' class='note-pdf' style='display:none;'>
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Latest revision as of 22:04, 17 October 2014

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Lab notes

labnote 1

labnote 2

labnote 3

labnote 4

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