Team:NYMU-Taipei/modeling/m6

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New part:Target

Introduction

This part aims to simulate the circuit of the Target part, which will be turn on when S. mutans is too much or phage is unable to control the S. mutans quorum.

circuit圖

LuxI coding sequence is controlled by nlmC promoter and threshold terminator. When the quorum of S. mutans is too much, the LuxI will be expressed and generate AHL-synthase. LuxR, on the other hand, is generated by E. coli constitutively. The LuxR and AHL can form a complex that can activate the pLuxR promoter. In our design, pLuxR promoter in E. coli can activate the lysine protein that can kill S. mutans only.
We model this part to see how the circuit works if phage is unable to control the S. mutans quorum, so the initial state of comE, the molecule that triggers the nlmC promoter, has a certain amount, which means the S. mutans is out of control.

System

Result

Reference

New part2

method

result

Reference