"
Team:NYMU-Taipei/modeling/m3
From 2014.igem.org
(Difference between revisions)
| Line 8: | Line 8: | ||
<h1>system</h1> | <h1>system</h1> | ||
<p>$$\frac{d[\text{lysis gene mRNA}]}{dt} = \beta\frac{[\text{comE}]^n}{K_d+[\text{comE}]^n}(1-a)^w-[\text{lysis gene mRNA}]K_m$$</p> | <p>$$\frac{d[\text{lysis gene mRNA}]}{dt} = \beta\frac{[\text{comE}]^n}{K_d+[\text{comE}]^n}(1-a)^w-[\text{lysis gene mRNA}]K_m$$</p> | ||
| + | <p>$$\frac{d[\text{lysis protein}]}{dt} = K_t[\text{lysis gene mRNA}]-K_p[\text{lysis protein}]$$</p> | ||
| + | <p>$\beta$:max nlmC promoter activity</p> | ||
| + | <p>$K_d$:promoter-TF dissociation constant</p> | ||
| + | <p>$K_m$:lysis gene mRNA degradation</p> | ||
| + | <p>$K_t$:translation efficiency</p> | ||
| + | <p>$K_p$:lysis protein degradation</p> | ||
| + | <p>$n$:hill coefficient</p> | ||
| + | <p>$a$:terminator efficiency</p> | ||
| + | <p>$w$:amount of terminator</p> | ||
<h1>result</h1> | <h1>result</h1> | ||
<h1>discussion</h1> | <h1>discussion</h1> | ||
</div> | </div> | ||
</html> | </html> | ||
Revision as of 11:33, 5 August 2014
X
Next ⇒
⇐ Prev
...
purpose & introduction
How to control the starting time of phage killing?
We use multiple terminators to make a threshold of the production of endolysin, so we build this model to determine the number of terminators.
system
$$\frac{d[\text{lysis gene mRNA}]}{dt} = \beta\frac{[\text{comE}]^n}{K_d+[\text{comE}]^n}(1-a)^w-[\text{lysis gene mRNA}]K_m$$
$$\frac{d[\text{lysis protein}]}{dt} = K_t[\text{lysis gene mRNA}]-K_p[\text{lysis protein}]$$
$\beta$:max nlmC promoter activity
$K_d$:promoter-TF dissociation constant
$K_m$:lysis gene mRNA degradation
$K_t$:translation efficiency
$K_p$:lysis protein degradation
$n$:hill coefficient
$a$:terminator efficiency
$w$:amount of terminator
