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Safety
Organisms and Parts
Organisms
This year, we used different species of E.coli in our project. Different Genotype provided us different choices when planning our experiment. Based on our project, following 4 species of E.coli was used in our project. All of these species are common and widely used in the field of molecular clone.
| Strain | Species name | Genotype | Rish Group | Rish Group Source | Disease risk to humans |
| DH5α | Escherichia coli | F-，φ80dlacZΔM15，Δ(lacZYA-argF)U169，deoR，recA1，endA1，hsdR17(rk-，mk+)，phoA，supE44，λ-，thi-1，gyrA96，relA1 | 1 | PSDS | low infectious dose |
| Top10 | Escherichia coli | F-, mcrA, Δ(mrr-hsdRMS-mcrBC), φ80lacZΔM15, ΔlacX74, nupG, recA1, araD139, Δ(ara-leu)7697, galE15, galK16, rpsL(StrR), endA1, λ- | 1 | PSDS | low infectious dose |
| BL21（DE3） | Escherichia coli | F-，ompT， hsdS（rBB-mB－），gal， dcm（DE3） | 1 | PSDS | low infectious dose |
| MC1061/P3 | Escherichia coli | F-, hsdR, (rk-, mk+) araD139, Δ(araABC-leu)7679, galU, galK, ΔlacX74, rpsL, thi, mcrB, {P3: KanR, AmpR,(am) TetR, (am)} | 1 | PSDS | low infectious dose |
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Parts
Our parts are all assembled with materials from the distribution or synthesized based on known sequence.
For more detaile, please read the ’’part’’ section of our wiki.
Institutional Rules and Regulations
The NUDT's Commission for Construction of Biological Sciences is responsible for bio-security in our institution. We have discussed the project with the teachers of this commission, and gotten their approval. Three extra teachers led by the deputy chief of DCB(the Department of Chemistry and Biology) were designated to provide us assistance if necessary and monitor us on our implementation on the lab bio-safety protocols at the same time.
Training
All team members were required to attend safety training focused on:
- General biosafety principles.
- The proper use of containment equipment (Biological safety (laminar flow) cabinets and delimited working space when required) as well as personal protective equipment (PPE) (White coat and gloves).
- Risk assessment of experiments involving: Recombinant DNA, ethidium bromide, other bio hazardous materials.
- Proper Biological waste disposal.
Lab security
toxic chemical reagents EB
Ethidium Bromide (EB) is a very toxic agent. However, because it’s high efficiency and low cost, our lab still use this molecule as nucleic acid staining molecule. To prevent it from hurting us, our lab requires everyone in the electrophoresis Rome to wear a second pair of gloves. Also, Wasted gel were collected and properly disposed.
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