Team:NEAU-Harbin/Team2

From 2014.igem.org

(Difference between revisions)
(Created page with "test")
 
(2 intermediate revisions not shown)
Line 1: Line 1:
-
test
+
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
 +
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
 +
<meta name="generator" content="MediaWiki 1.16.0" />
 +
<link rel="shortcut icon" href="/favicon.ico" />
 +
<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2012.igem.org (en)" />
 +
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:Cornell/protocols&amp;action=creativecommons" rel="meta" />
 +
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" />
 +
<link rel="alternate" type="application/atom+xml" title="2012.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&amp;feed=atom" /> <title>Team:Cornell/protocols - 2012.igem.org</title>
 +
<style type="text/css" media="screen, projection">/*<![CDATA[*/
 +
@import "/wiki/skins/common/shared.css?270";
 +
@import "/wiki/skins/igem/main.css?270";
 +
/*]]>*/</style>
 +
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" />
 +
<!--[if lt IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE50Fixes.css?270";</style><![endif]-->
 +
<!--[if IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE55Fixes.css?270";</style><![endif]-->
 +
<!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?270";</style><![endif]-->
 +
<!--[if IE 7]><style type="text/css">@import "/wiki/skins/igem/IE70Fixes.css?270";</style><![endif]-->
 +
<!--[if lt IE 7]><script type="text/javascript" src="/wiki/skins/common/IEFixes.js?270"></script>
 +
<meta http-equiv="imagetoolbar" content="no" /><![endif]-->
 +
 +
<script>
 +
var skin="igem",
 +
stylepath="/wiki/skins",
 +
wgUrlProtocols="http\\:\\/\\/|https\\:\\/\\/|ftp\\:\\/\\/|irc\\:\\/\\/|gopher\\:\\/\\/|telnet\\:\\/\\/|nntp\\:\\/\\/|worldwind\\:\\/\\/|mailto\\:|news\\:|svn\\:\\/\\/",
 +
wgArticlePath="/$1",
 +
wgScriptPath="/wiki",
 +
wgScriptExtension=".php",
 +
wgScript="/wiki/index.php",
 +
wgVariantArticlePath=false,
 +
wgActionPaths={},
 +
wgServer="https://2012.igem.org",
 +
wgCanonicalNamespace="",
 +
wgCanonicalSpecialPageName=false,
 +
wgNamespaceNumber=0,
 +
wgPageName="Team:Cornell/protocols",
 +
wgTitle="Team:Cornell/protocols",
 +
wgAction="view",
 +
wgArticleId=36204,
 +
wgIsArticle=true,
 +
wgUserName=null,
 +
wgUserGroups=null,
 +
wgUserLanguage="en",
 +
wgContentLanguage="en",
 +
wgBreakFrames=false,
 +
wgCurRevisionId=282624,
 +
wgVersion="1.16.0",
 +
wgEnableAPI=true,
 +
wgEnableWriteAPI=true,
 +
wgSeparatorTransformTable=["", ""],
 +
wgDigitTransformTable=["", ""],
 +
wgMainPageTitle="Main Page",
 +
wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2012.igem.org", "5": "2012.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"},
 +
wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2012.igem.org": 4, "2012.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},
 +
wgSiteName="2012.igem.org",
 +
wgCategories=[],
 +
wgMWSuggestTemplate="https://2012.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest",
 +
wgDBname="2012_igem_org",
 +
wgSearchNamespaces=[0],
 +
wgMWSuggestMessages=["with suggestions", "no suggestions"],
 +
wgRestrictionEdit=[],
 +
wgRestrictionMove=[];
 +
</script>               
 +
<script type="text/javascript" src="/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script>
 +
<!-- Head Scripts -->
 +
<script src="/wiki/skins/common/ajax.js?270"></script>
 +
<script src="/wiki/skins/common/mwsuggest.js?270"></script>
 +
<script type="text/javascript" src="/wiki/index.php?title=-&amp;action=raw&amp;gen=js&amp;useskin=igem"><!-- site js --></script>
 +
<!-- jQuery Javascript -->
 +
<script type='text/javascript'        src ='/common/jquery-latest.min.js'></script>
 +
<script type='text/javascript'        src ='/common/tablesorter/jquery.tablesorter.min.js'></script>
 +
        <link rel='stylesheet' type='text/css' href='/common/tablesorter/themes/groupparts/style.css' />
 +
        <link rel='stylesheet' type='text/css' href='/common/table_styles.css' />
 +
        <link rel='stylesheet' type='text/css' href='/forum/forum_styles.css' />
 +
<script type='text/javascript'        src ='/forum/forum_scripts.js'></script>
 +
</head>
 +
 +
<body              class="mediawiki  ltr ns-0 ns-subject page-Team_Cornell_protocols">
 +
  <div id="globalWrapper">
 +
 +
    <div id="top-section">
 +
<div id="p-logo">
 +
    <a href="/Main_Page"
 +
      title="Main Page">
 +
    <img src='/wiki/skins/common/images/wiki.png'>"
 +
    </a>
 +
</div>  <!-- end p-logo -->
 +
<script type="text/javascript"> if (window.isMSIE55) fixalpha(); </script>
 +
 +
 +
 +
<div id="menubar" class='left-menu noprint'>
 +
  <ul>
 +
                  <li
 +
                class='selected'       ><a href="/Team:Cornell/protocols">Page              </a></li>
 +
              <li
 +
                class='new'       ><a href="/wiki/index.php?title=Talk:Team:Cornell/protocols&amp;action=edit&amp;redlink=1">Discussion              </a></li>
 +
              <li
 +
                    ><a href="/wiki/index.php?title=Team:Cornell/protocols&amp;action=edit">View source              </a></li>
 +
              <li
 +
                    ><a href="/wiki/index.php?title=Team:Cornell/protocols&amp;action=history">History              </a></li>
 +
              <li style='color:#808080;cursor:default'>teams</li>
 +
  </ul>
 +
</div> <!-- end menubar (left) -->
 +
 +
<div class="right-menu noprint" id="menubar">
 +
    <ul>
 +
                <li id="pt-login"><a href="/wiki/index.php?title=Special:UserLogin&amp;returnto=Team:Cornell/protocols" title="You are encouraged to log in; however, it is not mandatory [o]" accesskey="o">Log in</a></li>     </ul>
 +
</div><!-- end right menubar -->
 +
 +
<div id="search-controls" class="noprint">
 +
<form action="/Special:Search" id="searchform">
 +
<input id="searchInput" name="search" type="text" title="Search 2012.igem.org [f]" accesskey="f" value="" />
 +
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;
 +
      <input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" />
 +
</form>
 +
</div> <!-- close search controls -->
 +
    </div> <!-- close top-section-->
 +
    <div id="content">
 +
<a name="top" id="top"></a>
 +
<h1 class="firstHeading">Team:Cornell/protocols</h1>
 +
<div id="bodyContent">
 +
<h3 id="siteSub" class='noprint'>From 2012.igem.org</h3>
 +
<div id="contentSub"></div>
 +
<!--
 +
<div id="jump-to-nav">Jump to:                        <a href="#column-one">navigation</a>, <a href="#searchInput">search</a></div>-->
 +
<!-- start content -->
 +
<p>
 +
<!--<![endif]-->
 +
<head>
 +
<meta charset="utf-8" />
 +
<meta name="viewport" content="width=device-width" />
 +
<link rel="stylesheet" href="https://2012.igem.org/Team:Cornell/stylesheets/foundation?action=raw&amp;ctype=text/css">
 +
<link rel="stylesheet" href="https://2012.igem.org/Team:Cornell/stylesheets/app?action=raw&amp;ctype=text/css">
 +
<script src="https://2012.igem.org/Team:Cornell/javascripts/modernizr.foundation?action=raw&amp;ctype=text/javascript"></script>
 +
<!-- IE Fix for HTML5 Tags -->
 +
<!--[if lt IE 9]>
 +
<script src="http://html5shiv.googlecode.com/svn/trunk/html5.js"></script>
 +
<![endif]-->
 +
<style>
 +
#footer-box {
 +
visibility: hidden;
 +
}
 +
</style>
 +
<script>
 +
function fixTour() {
 +
var links=new Array();
 +
links[0]="https://2012.igem.org/Team:Cornell/templates/tour";
 +
links[1]="https://2012.igem.org/Team:Cornell/Sandbox/project/wetlab";
 +
links[2]="somepage";
 +
var prev = document.getElementById("tourPrev");
 +
var next = document.getElementById("tourNext");
 +
var start = document.getElementById("tourStart");
 +
start.href=links[0];
 +
var needprev = false;
 +
var neednext = false;
 +
for(var i=0;i<links.length;i++) {
 +
if(window.location.href.toString()==links[i]) {
 +
if(i==0) {
 +
neednext = true;
 +
next.href=links[i+1];
 +
 +
}
 +
else if(i==links.length-1) {
 +
needprev = true;
 +
prev.href=links[i-1];
 +
}
 +
else {
 +
neednext = true;
 +
needprev = true;
 +
prev.href=links[i-1];
 +
next.href=links[i+1];
 +
}
 +
}
 +
}
 +
if(needprev) prev.parentNode.style.display="block";
 +
if(neednext) next.parentNode.style.display="block";
 +
else if(!needprev) start.parentNode.style.display="block";
 +
}
 +
</script>
 +
</head>
 +
<body onload="fixTour()">
 +
<!-- TOUR TEMPLATE
 +
<div class="tour" style="left:0;display:none">
 +
<a id="tourPrev" class="button radius"> Previous<br/>Tour Page </a>
 +
</div>
 +
<div class="tour" style="right:0;display:none">
 +
<a id="tourNext" class="button radius"> Next<br/>Tour Page </a>
 +
</div>
 +
<div class="tour" style="right:0;display:none">
 +
<a id="tourStart" class="button radius"> Start<br/>Tour! </a>
 +
</div>
 +
HEADER TEMPLATE -->
 +
<div class="row header off-canvas">
 +
<div class="twelve columns centered header no-pad" id="header-wrapper">
 +
<a href="https://2012.igem.org/Team:Cornell">
 +
<div id="logo-wrapper">
 +
<img src="https://static.igem.org/mediawiki/2012/3/3b/CornelliGEMLogo.jpg">
 +
</div> </a>
 +
<a href="https://2012.igem.org/Main_Page">
 +
<div id="igem-attr">
 +
<img src="https://static.igem.org/mediawiki/igem.org/6/67/IGEMsmall.png">
 +
</div> </a>
 +
<ul class="nav-bar">
 +
<li class="pg-home">
 +
<a href="https://2012.igem.org/Team:Cornell">Home</a>
 +
</li>
 +
<li class="has-flyout pg-project pg-project_background pg-project_wetlab pg-project_drylab pg-project_hprac pg-project_gallery pg-project_safety">
 +
<a href="https://2012.igem.org/Team:Cornell/project">Project</a>
 +
<a href="https://2012.igem.org/Team:Cornell/project" class="flyout-toggle"><span> </span></a>
 +
<ul class="flyout">
 +
<li class="pg-project">
 +
<a href="https://2012.igem.org/Team:Cornell/project">Overview</a>
 +
</li>
 +
<li class="pg-project_background">
 +
<a href="https://2012.igem.org/Team:Cornell/project/background">Background</a>
 +
</li>
 +
<li class="pg-project_wetlab">
 +
<a href="https://2012.igem.org/Team:Cornell/project/wetlab">Wet Lab</a>
 +
</li>
 +
<li class="pg-project_drylab">
 +
<a href="https://2012.igem.org/Team:Cornell/project/drylab">Dry Lab</a>
 +
</li>
 +
<li class="pg-project_hprac">
 +
<a href="https://2012.igem.org/Team:Cornell/project/hprac">Human Practices</a>
 +
</li>
 +
<li class="pg-project_gallery">
 +
<a href="https://2012.igem.org/Team:Cornell/project/gallery">Gallery</a>
 +
</li>
 +
<li class="pg-project_safety">
 +
<a href="https://2012.igem.org/Team:Cornell/project/hprac/safety">Safety</a>
 +
</li>
 +
</ul>
 +
</li>
 +
<li class="has-flyout pg-notebook pg-notebook_wetlab pg-notebook_drylab pg-notebook_strainlist">
 +
<a href="https://2012.igem.org/Team:Cornell/notebook">Notebook</a>
 +
<a href="https://2012.igem.org/Team:Cornell/notebook" class="flyout-toggle"><span> </span></a>
 +
<ul class="flyout">
 +
<li class="pg-notebook">
 +
<a href="https://2012.igem.org/Team:Cornell/notebook">Overview</a>
 +
</li>
 +
<li class="pg-notebook_wetlab">
 +
<a href="https://2012.igem.org/Team:Cornell/notebook/wetlab">Wet Lab</a>
 +
</li>
 +
<li class="pg-notebook_drylab">
 +
<a href="https://2012.igem.org/Team:Cornell/notebook/drylab/june">Dry Lab</a>
 +
</li>
 +
<li class="pg-notebook_strainlist">
 +
<a href="https://2012.igem.org/Team:Cornell/notebook/strain_list" target="_blank">Strain List</a>
 +
</li>
 +
</ul>
 +
</li>
 +
<li class="pg-protocols">
 +
<a href="https://2012.igem.org/Team:Cornell/protocols">Protocols</a>
 +
</li>
 +
<li class="has-flyout pg-team pg-team_bios pg-team_sponsors">
 +
<a href="https://2012.igem.org/Team:Cornell/team">Team</a>
 +
<a href="https://2012.igem.org/Team:Cornell/team" class="flyout-toggle"><span> </span></a>
 +
<ul class="flyout right">
 +
<li class="pg-team">
 +
<a href="https://2012.igem.org/Team:Cornell/team">Overview</a>
 +
</li>
 +
<li class="pg-team_bios">
 +
<a href="https://2012.igem.org/Team:Cornell/team/bios">Bios</a>
 +
</li>
 +
<li>
 +
<a href="https://igem.org/Team.cgi?year=2012&amp;team_name=Cornell" target="_blank">Profile</a>
 +
</li>
 +
<li class="pg-team_sponsors">
 +
<a href="https://2012.igem.org/Team:Cornell/team/sponsors">Sponsors</a>
 +
</li>
 +
</ul>
 +
</li>
 +
<li class="has-flyout pg-outreach pg-outreach_presentations pg-outreach_panels pg-outreach_awareness">
 +
<a href="https://2012.igem.org/Team:Cornell/outreach">Outreach</a>
 +
<a href="https://2012.igem.org/Team:Cornell/outreach" class="flyout-toggle"><span> </span></a>
 +
<ul class="flyout right">
 +
<li class="pg-outreach">
 +
<a href="https://2012.igem.org/Team:Cornell/outreach">Overview</a>
 +
</li>
 +
<li class="pg-outreach_presentations">
 +
<a href="https://2012.igem.org/Team:Cornell/outreach/presentations">Research Presentations</a>
 +
</li>
 +
<li class="pg-outreach_panels">
 +
<a href="https://2012.igem.org/Team:Cornell/outreach/panels">Panel Discussions</a>
 +
</li>
 +
<li class="pg-outreach_awareness">
 +
<a href="https://2012.igem.org/Team:Cornell/outreach/awareness">Public Awareness</a>
 +
</li>
 +
</ul>
 +
</li>
 +
<li class="has-flyout">
 +
<a style="visibility:none"></a>
 +
<a href="https://2012.igem.org/Team:Cornell">
 +
<div class="badge-wrapper-main">
 +
<img style="margin-left:2px;margin-top:-1px;height:65px;" src="https://static.igem.org/mediawiki/2012/7/70/Cornell2012Badge.png">
 +
</div> </a>
 +
<a href="https://2012.igem.org/Team:Cornell"><span> </span></a>
 +
<ul class="flyout right" style="display:none;background:none;border:none;-webkit-box-shadow:none;-moz-box-shadow:none;box-shadow:none">
 +
<li class="pg-badge">
 +
<div class="badge-wrapper">
 +
<a href="https://2013.igem.org/Team:Cornell">
 +
<img style="margin-left:2px;margin-top:-1px;height:65px;" src="https://static.igem.org/mediawiki/2012/7/70/Cornell2012Badge.png">
 +
</a>
 +
</div>
 +
<a href="https://2013.igem.org/Team:Cornell" style="margin-top:-50px">2012: SAFE BET</a>
 +
</li>
 +
<li class="pg-badge_home">
 +
<div class="badge-wrapper">
 +
<a href="http://igem.engineering.cornell.edu">
 +
<img style="margin-left: 2px;margin-top: 2px;height: 56px;" src="http://igem.engineering.cornell.edu/img/CornellBadgeLogo2.png">
 +
</a>
 +
</div>
 +
<a href="http://igem.engineering.cornell.edu">Team Website</a>
 +
</li>
 +
<li class="pg-badge_2013">
 +
<div class="badge-wrapper">
 +
<a href="https://2013.igem.org/Team:Cornell">
 +
<img style="margin-left:7px;margin-top:2px;height:56px;" src="https://static.igem.org/mediawiki/2013/1/1c/CornellOrganofoamBadge.png">
 +
</a>
 +
</div>
 +
<a href="https://2013.igem.org/Team:Cornell">2013: Organofoam</a>
 +
</li>
 +
<li class="pg-badge_2011">
 +
<div class="badge-wrapper">
 +
<a href="https://2011.igem.org/Team:Cornell">
 +
<img style="margin-left:4px;margin-top:8px;height:44px;" src="https://static.igem.org/mediawiki/2013/4/46/CornellBiofactory.png">
 +
</a>
 +
</div>
 +
<a href="https://2011.igem.org/Team:Cornell">2011: Biofactory</a>
 +
</li>
 +
<li class="pg-badge_2010">
 +
<div class="badge-wrapper">
 +
<a href="https://2010.igem.org/Team:Cornell">
 +
<img style="margin-left:-3px;margin-top:6px;height:60px;" src="https://static.igem.org/mediawiki/2013/3/3c/CornellOmgOmvs.png">
 +
</a>
 +
</div>
 +
<a href="https://2010.igem.org/Team:Cornell">2010: OMG OMVs</a>
 +
</li>
 +
<li class="pg-badge_2009">
 +
<div class="badge-wrapper">
 +
<a href="https://2009.igem.org/Team:Cornell">
 +
<img style="margin-left:0px;height:60px;" src="https://static.igem.org/mediawiki/2013/a/a0/CornellCadmium.png">
 +
</a>
 +
</div>
 +
<a href="https://2009.igem.org/Team:Cornell" style="font-size:20px !important">2009: Cadmium Sensor</a>
 +
</li>
 +
</ul>
 +
</li>
 +
</ul>
 +
<div class="clearfix"></div>
 +
</div>
 +
</div>
 +
 +
 +
<!--<![endif]-->
 +
<div class="row main-image">
 +
<div class="twelve columns">
 +
<img src="https://static.igem.org/mediawiki/2012/9/93/Swati_and_Claire.jpg">
 +
<a class="button radius gallery-button" href="https://2012.igem.org/Team:Cornell/project/gallery">More Photos</a>
 +
</div>
 +
</div>
 +
 +
<div class="row">
 +
<div class="four columns">
 +
<h4>DNA Purification</h4>
 +
<ul>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/4/46/EZNA_Gel_Extraction.pdf" target="_blank">EZNA Gel Extraction</a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/3/35/Ezna_Miniprep.pdf" target="_blank">EZNA Miniprep</a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/2/21/Qiagen_Gel_Extraction.pdf" target="_blank">Qiagen Gel Extraction</a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/2/2d/Qiagen_Miniprep.pdf" target="_blank">Qiagen Miniprep</a>
 +
</li>
 +
</ul>
 +
</div>
 +
<div class="four columns">
 +
<h4>DNA Assessment</h4>
 +
<ul>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/6/6c/DNA_Gel_Electrophoresis.pdf" target="_blank">DNA Gel Electrophoresis</a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/0/03/Sequencing_Preparation.pdf" target="_blank">Preparation of DNA for Sequencing</a>
 +
</li>
 +
 +
</ul>
 +
</div>
 +
<div class="four columns">
 +
<h4>PCR Methods</h4>
 +
<ul>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/2/26/Phusion_PCR.pdf" target="_blank">Phusion PCR</a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/4/4b/Taq_PCR.pdf" target="_blank">Taq PCR</a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/3/33/Vent_PCR.pdf" target="_blank">Vent PCR</a>
 +
</li>
 +
</ul>
 +
</div>
 +
</div>
 +
<div class="row">
 +
<div class="four columns">
 +
<h4>Plasmid Insertion</h4>
 +
<ul>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/0/07/Ecoli_electroporation.pdf" target="_blank">Electroporation of <i>E.coli</i></a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/5/57/Making_Ecoli_Electrocompetent.pdf" target="_blank">Preparing Electrocompetent <i>E.coli</i></a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/6/6f/MyersMyers_Shew-E-poration.pdf" target="_blank">Myers and Myers Electoporation of <i>Shewanella</i></a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/f/f7/PNNL_Electrocompetent_Shewanella.pdf" target="_blank">PNNL’s Preparing Electrocompetent <i>Shewanella</i></a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/0/0f/Shewanella_Conjugation.pdf" target="_blank">Conjugation of Plasmids into <i>Shewanella</i> </a>
 +
</li>
 +
</ul>
 +
</div>
 +
<div class="four columns">
 +
<h4>Plasmid Editing</h4>
 +
<ul>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/a/af/Double_Digest.pdf" target="_blank">Double Digest</a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/f/fb/Single_Digest.pdf" target="_blank">Single Digest</a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/a/a5/Site_Directed_Mutagenesis.pdf" target="_blank">Site Directed Mutagenesis</a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/a/a7/T4_Ligase_Ligation.pdf" target="_blank">T4 Ligase Ligation</a>
 +
</li>
 +
</ul>
 +
</div>
 +
<div class="four columns">
 +
<h4><i>Shewanella</i> Growth</h4>
 +
<ul>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/6/6c/Growth_Assay_Protocol.pdf" target="_blank"><i>Shewanella</i> Growth Assay Protocol</a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/b/bc/M4_recipe.pdf" target="_blank">M4 Recipe</a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/c/cb/Synthetic_River_Media.pdf" target="_blank">Synthetic River Media Recipe</a>
 +
</li>
 +
<li>
 +
<a href="https://2012.igem.org/Team:Cornell/protocols/secret_dylan_dance" target="_blank">Plate Drying</a>
 +
</li>
 +
</ul>
 +
</div>
 +
</div>
 +
 +
<script src="https://2012.igem.org/Team:Cornell/javascripts/foundation.min?action=raw&amp;ctype=text/javascript"></script>
 +
<script src="https://2012.igem.org/Team:Cornell/javascripts/app?action=raw&amp;ctype=text/javascript"></script>
 +
<script type="text/javascript">
 +
$(window).load(function() {
 +
$('li.pg-protocols').addClass('active');
 +
});
 +
</script>
 +
</body>
 +
 +
</p>
 +
<!--
 +
NewPP limit report
 +
Preprocessor node count: 9/1000000
 +
Post-expand include size: 42/2097152 bytes
 +
Template argument size: 0/2097152 bytes
 +
Expensive parser function count: 0/100
 +
-->
 +
 +
<!-- Saved in parser cache with key 2012_igem_org:pcache:idhash:36204-0!1!0!!en!2!edit=0 and timestamp 20140927033231 -->
 +
<div class="printfooter">
 +
Retrieved from "<a href="https://2012.igem.org/Team:Cornell/protocols">https://2012.igem.org/Team:Cornell/protocols</a>"</div>
 +
<div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content -->
 +
<div class="visualClear"></div>
 +
</div>
 +
    </div>
 +
<!-- PAGE FOOTER -- ITEMS FROM COLUMN ! HAVE BEEN MOVED HERE  -- RDR  -->
 +
<div class="visualClear"></div>
 +
    <div id='footer-box' class='noprint'>
 +
        <div id="footer">
 +
              <div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div>       <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by/3.0/"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div>     <ul id="f-list">
 +
 +
 +
  <!-- Recentchanges is not handles well DEBUG -->
 +
    <li id="t-recentchanges"><a href="/Special:RecentChanges"
 +
      title='Recent changes'>Recent changes</a></li>
 +
 +
    <li id="t-whatlinkshere"><a href="/Special:WhatLinksHere/Team:Cornell/protocols"
 +
      title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li>
 +
 +
                        <li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Team:Cornell/protocols"
 +
                          title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a></li>
 +
 +
 +
 +
                <li id="t-specialpages"><a href="/Special:SpecialPages"
 +
                  title="List of all special pages [q]" accesskey="q">Special pages</a>
 +
                </li>
 +
                <li><a href='/Special:Preferences'>My preferences</a></li>
 +
            </ul>
 +
        </div> <!-- close footer -->
 +
        <div id='footer'>
 +
    <ul id="f-list">
 +
 +
            <li id="t-print"><a href="/wiki/index.php?title=Team:Cornell/protocols&amp;printable=yes"
 +
              title="Printable version of this page [p]" accesskey="p">Printable version</a>
 +
            </li>
 +
 +
            <li id="t-permalink"><a href="/wiki/index.php?title=Team:Cornell/protocols&amp;oldid=282624"
 +
              title="Permanent link to this revision of the page">Permanent link</a>
 +
            </li>
 +
 +
 +
        <li id="privacy"><a href="/2012.igem.org:Privacy_policy" title="2012.igem.org:Privacy policy">Privacy policy</a></li>
 +
        <li id="disclaimer"><a href="/2012.igem.org:General_disclaimer" title="2012.igem.org:General disclaimer">Disclaimers</a></li>
 +
    </ul>
 +
</div> <!-- close footer -->
 +
    </div> <!-- close footer-box -->
 +
 +
<script>if (window.runOnloadHook) runOnloadHook();</script>
 +
</div>
 +
<!-- Served in 0.172 secs. --></body>
 +
 +
</html>

Latest revision as of 04:07, 27 September 2014

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Team:Cornell/protocols - 2012.igem.org

 

Team:Cornell/protocols

From 2012.igem.org

More Photos

DNA Purification

DNA Assessment

PCR Methods

Plasmid Insertion

Plasmid Editing

Shewanella Growth

Retrieved from "https://2012.igem.org/Team:Cornell/protocols"

Retrieved from "http://2014.igem.org/Team:NEAU-Harbin/Team2"