Team:NCTU Formosatest

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            <li class="navli" style="background-color:#ffffff"><navtext>Project</navtext></li>
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            <li class="navli">Background</navtext></li>
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            <li class="navli"><navtext>Design</navtext></li>
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            <li class="navli"><navtext>Result</navtext></li>
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            <li class="navli"><navtext>Modeling</navtext></li>
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<div class="TEXT" >Design</div>
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<div class="TEXT" >Result</div>
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<div class="TEXT" >Modeling</div>
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===Background /===
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<img src="https://static.igem.org/mediawiki/2015/8/8e/2015NCTU_Formosa_modeling.png" title="klematis flower" width="150" height="113">
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Our project focuses on the diagnoses before and after treatment for cancers.
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        <div class="textbox">Our project focuses on the diagnoses before and after treatment for cancers. Two systems are constructed for our project. The first system displays scFv on the surface of E.coli while the second one displays gold binding peptide (GBP). 
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Before treatment, by using the fluorescent E.coli with scFv displayed on the surface, IHC procedure can be simplified because secondary antibodies is omitted and solves the problem of non-specific binding of secondary antibodies.
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  A dual system involving E.coli displaying scFv and GBP can solve random orientation of antibodies coating on gold chip to attain an accurate measurement. Therefore, the dual system can also offer a quantitative and accurate diagnosis for after treatment of cancer.
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Our project not only improves the diagnoses of the cancer types before treatment easily but also helps track the patient's condition after treatment by multifaceted measurement technology with more accurate quantitative results!
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        <div class="upper"><g1>Design / </g1><g2>To display scfv on the E.coli's surface we used a transmembrance fusion protein.</g2>
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        <div class="textbox">ScFv is a single chain variable fragment the binding site for antigen. Comparing to the entire antibody, scFv is smaller for E.coli to display it.To display scfv on the E.coli’s surface we used a transmembrance fusion protein. This fusion protein is composed of Lipoprotein and a small part of outermembrane protein A (OmpA). Its C terminal will connect with the scFv and display it onto the membrane surface of the E.coli.
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            <div class="upper"><g1>Result / Sucessfully present our E.cotector</g2>
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    <div class="upper"><g1>Modeling / </g1><g2>Find out the best condition of our E.cotector</g2>
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Revision as of 06:06, 28 August 2015

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None

4/11

Transformation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)

Transformation of [Plac+PSB1A3] Ar, [mGFP+PSB1A3] Ar (G3)

Transformation of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr (G1)

4/12

Cultivation of [PSB1K3] Kr, [PSB1C3] Cr (G2)

Cultivation of [PSB1A3] Ar, [PSB1C3] Cr (G1)

Cultivation of [mGFP] Ar (G3)


4/13

Mini-prep of [mGFP] Ar, [PSB1K3] Kr (G3)

Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2)

Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)

4/17

Transformation of [PSB1K3] Kr (G1)

4/18

Cultivation of [PSB1K3] Kr (G1)

4/19

Transformation of [Plac+PSB1A3] Ar, [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G3)

Mini-prep of [PSB1K3] Kr (G1)


4/20

Transformation of [PSB1A3] Ar (G2)

Electrophoresis of [PSB1K3] Kr, [PSB1C3] Cr plasmid (G2)

4/21

Cultivation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)

4/22

Mini-prep of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)


4/28

Electrophoresis of digested [PSB1K3] Kr(EP), [PSB1C3] Cr(EP),[PSB1A3] Ar(EP) (G1)

Cultivation of [PSB1C3] Cr (G1)

4/29

Mini-prep of [PSB1C3] Cr (G1)

Mini-prep of [PSB1C3] Cr (G2)

Transformation of [B0032] Ar, and [BFP] Cr (G2)

Cultivation of [PSB1K3] Kr, [PSB1A3] Ar (G1)

Cultivation of [B0032] Ar, and [BFP] Cr (G2)

4/30

Mini-prep of [PSB1A3] Ar (G1)

Mini-prep of [B0032] Ar, and [BFP] Cr (G2)

Transformation of [B0032] Ar, and [BFP] Cr, [T7promoter] Ar Kr and [T7 polymerase] Cr (G2)

Transformation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)

Digestion of [PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)

Electrophoresis of plasmid and digested prouct of[PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)


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