Team:NCTU Formosa/biobricks

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Project

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Parts submitted to the Registry

<groupparts>iGEM014 NCTU_Formosa</groupparts>

Brief Information

Please click on the name of the parts for detailed information that is hosted in the Registry website. Team:NCTU Formosa/file/test

PBAN-producted system

PBAN Bombyx mori 1
BM.png

Peptide Sequence: LSEDMPATPADQEMYQPDPEEMESRTRYFSPRL

PBAN(BM)+pSB1C3 This biobrick is the PBAN of Bombyx mori on the pSB1C3 backbone.

PBAN Bombyx mori 2
HALFBM.png

Pcons(J23101) + RBS(B0034) + PBAN(BM) This biobrick can produce Bombyx mori’s PBAN. As Bombyx mori’s PBAN is ingested by the female Bombyx mori, the female Bombyx mori will produce its own sex pheromone and attract the male Bombyx mori.

PBAN Bombyx mori 3
ALLBM.png

Pcons(J23101) + RBS(B0034) + PBAN(BM) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick produces Bombyx mori’s PBAN or not. If our biobrick works, it would be shown through the expression of the BFP gene and the emission of blue fluorescent light.

PBAN Mamestra brassicae 1
MB.png

Peptide Sequence: LADDMPATPADQEMYRPDPEQIDSRTKYFSPRL

PBAN(MB)+pSB1C3 This biobrick is the PBAN of Mamestra brassicae on the pSB1C3 backbone.

PBAN Mamestra brassicae 2
HALFMB.png

Pcons(J23101) + RBS(B0034) + PBAN(MB) This biobrick can produce Mamestra brassicae’s PBAN. As Mamestra brassicae’s PBAN is ingested by the female Mamestra brassicae, the female Mamestra brassicae will produce its own sex pheromone and attract the male Mamestra brassicae.

PBAN Mamestra brassicae 3
ALLMB.png

Pcons(J23101) + RBS(B0034) + PBAN(MB) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick produces Mamestra brassicae’s PBAN or not. If our biobrick works, it would be shown through the expression of the BFP gene and the emission of blue fluorescent light.

PBAN Agrotis ipsilon 1
AI.png

Peptide Sequence: LADDTPATPADQEMYRPDPEQIDSRTKYFSPRL

PBAN(AI)+pSB1C3 This biobrick is the PBAN of Agrotis ipsilon on the pSB1C3 backbone.

PBAN Agrotis ipsilon 2
HALFAI.png

Pcons(J23101) + RBS(B0034) + PBAN(AI) This biobrick can produce Agrotis ipsilon’s PBAN. As Agrotis ipsilon’s PBAN is ingested by the female Agrotis ipsilon, the female Agrotis ipsilon will produce its own sex pheromone and attract the male Agrotis ipsilon.

PBAN Agrotis ipsilon 3
ALLAI.png

Pcons(J23101) + RBS(B0034) + PBAN(AI) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick produces Agrotis ipsilon’s PBAN or not. If our biobrick works, it would be shown through the expression of the BFP gene and the emission of blue fluorescent light.

PBAN Lymantria dispar 1
LD.png

Peptide Sequence: LADDMPATMADQEVYRPEPEQIDSRNKUFSPRL

PBAN(AI)+pSB1C3 This biobrick is the PBAN of Lymantria dispar on the pSB1C3 backbone.

PBAN Lymantria dispar 2
HALFLD.png

Pcons(J23101) + RBS(B0034) + PBAN(LD) This biobrick can produce Lymantria dispar’s PBAN. As Lymantria dispar’s PBAN is ingested by the female Lymantria dispar, the female Lymantria dispar will produce its own sex pheromone and attract the male Lymantria dispar.

PBAN Lymantria dispar 3
ALLLD.png

Pcons(J23101) + RBS(B0034) + PBAN(LD) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick produces Lymantria dispar’s PBAN or not. If our biobrick works, it would be shown through the expression of the BFP gene and the emission of blue fluorescent light.

PBAN Spodoptera litura 1
SL.png

Peptide Sequence: LADDMPATPADQELYRPDPDQIDSRTKUFSPRL

PBAN(SL)+pSB1C3 This biobrick is the PBAN of Spodoptera litura on the pSB1C3 backbone.

PBAN Spodoptera litura 2
HALFSL.png

Pcons(J23101) + RBS(B0034) + PBAN(SL) This biobrick can produce Spodoptera litura’s PBAN. As Spodoptera litura’s PBAN is ingested by the female Spodoptera litura, the female Spodoptera litura will produce its own sex pheromone and attract the male Spodoptera litura.

PBAN Spodoptera litura 3
ALLSL.png

Pcons(J23101) + RBS(B0034) + PBAN(SL) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick produces Spodoptera litura’s PBAN or not. If our biobrick works, it would be shown through the expression of the BFP gene and the emission of blue fluorescent light.

PBAN Helicoverpa armigera Hubner 1
HAH.png

Peptide Sequence: LSDDMPARPADQEMYRQDPEQIDSRTKYFSPRL

PBAN(HAH)+pSB1C3 This biobrick is the PBAN of Helicoverpa armigera Hubner on the pSB1C3 backbone.

PBAN Helicoverpa armigera Hubner 2
HALFHAH.png

Pcons(J23101) + RBS(B0034) + PBAN(HAH) This biobrick can produce Helicoverpa armigera Hubner’s PBAN. As Helicoverpa armigera Hubner’s PBAN is ingested by the female Helicoverpa armigera Hubner, the female Helicoverpa armigera Hubner will produce its own sex pheromone and attract the male Helicoverpa armigera Hubner.

PBAN Helicoverpa armigera Hubner 3
ALLHAH.png

Pcons(J23101) + RBS(B0034) + PBAN(HAH) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick produces Helicoverpa armigera Hubner’s PBAN or not. If our biobrick works, it would be shown through the expression of the BFP gene and the emission of blue fluorescent light.

PBAN Adoxophyes sp. 1
AS.png

Peptide Sequence: QSEAVTSSDEQVYRQDMSPVDGRLKYFSPRL

PBAN(AS)+pSB1C3 This biobrick is the PBAN of Adoxophyes sp. on the pSB1C3 backbone.

PBAN Adoxophyes sp. 2
HALFAS.png

Pcons(J23101) + RBS(B0034) + PBAN(AS) This biobrick can produce Adoxophyes sp.’s PBAN. As Adoxophyes sp.’s PBAN is ingested by the female Adoxophyes sp., the female Adoxophyes sp. will produce its own sex pheromone and attract the male Adoxophyes sp.

PBAN Adoxophyes sp. 3
ALLAS.png

Pcons(J23101) + RBS(B0034) + PBAN(AS) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick produces Adoxophyes sp.’s PBAN or not. If our biobrick works, it would be shown through the expression of the BFP gene and the emission of blue fluorescent light.

PBAN Solenopsis invicta 1
SI.png

Peptide Sequence: GSGEDLSYGDAYEVDEDDHPLFVPR

PBAN(SI)+pSB1C3 This biobrick is the PBAN of Solenopsis invicta on the pSB1C3 backbone.

PBAN Solenopsis invicta 2
HALFSI.png

Pcons(J23101) + RBS(B0034) + PBAN(SI) This biobrick can produce Solenopsis invicta’s PBAN. As Solenopsis invicta’s PBAN is ingested by the female Solenopsis invicta, the female Solenopsis invicta will produce its own sex pheromone and attract the male Solenopsis invicta.

PBAN Solenopsis invicta 3
ALLSI.png

Pcons(J23101) + RBS(B0034) + PBAN(SI) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick produces Solenopsis invicta’s PBAN or not. If our biobrick works, it would be shown through the expression of the BFP gene and the emission of blue fluorescent light.

PBAN Aedes aegypti 1
AA.png

Peptide Sequence: DASSSNENNSRPPFAPRL

PBAN(AA)+pSB1C3 This biobrick is the PBAN of Aedes aegypti on the pSB1C3 backbone.

PBAN Aedes aegypti 2
HALFAA.png

Pcons(J23101) + RBS(B0034) + PBAN(AA) This biobrick can produce Aedes aegypti’s PBAN. As Aedes aegypti’s PBAN is ingested by the female Aedes aegypti, the female Aedes aegypti will produce its own sex pheromone and attract the male Aedes aegypti.

PBAN Aedes aegypti 3
ALLAA.png

Pcons(J23101) + RBS(B0034) + PBAN(AA) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick produces Aedes aegypti’s PBAN or not. If our biobrick works, it would be shown through the expression of the BFP gene and the emission of blue fluorescent light.