Team:NCTU Formosa/biobricks

From 2014.igem.org

(Difference between revisions)
(Brief Information)
(Brief Information)
Line 90: Line 90:
======PBAN Spodoptera litura 1======
======PBAN Spodoptera litura 1======
-
[[File:ALLLD.png|200px|thumb|center|alt text]]
+
[[File:SL.png|200px|thumb|center|alt text]]
Peptide Sequence:
Peptide Sequence:
LADDMPATPADQELYRPDPDQIDSRTKUFSPRL
LADDMPATPADQELYRPDPDQIDSRTKUFSPRL
Line 96: Line 96:
======PBAN Spodoptera litura 2======
======PBAN Spodoptera litura 2======
-
[[File:AA.png|200px|thumb|center|alt text]]
+
[[File:HALFSL.png|200px|thumb|center|alt text]]
<P>Pcons(J23101) + RBS(B0034) + PBAN(SL)  
<P>Pcons(J23101) + RBS(B0034) + PBAN(SL)  
This biobrick produces Spodoptera litura’s PBAN.</P>
This biobrick produces Spodoptera litura’s PBAN.</P>
======PBAN Spodoptera litura 3======
======PBAN Spodoptera litura 3======
-
[[File:AA.png|200px|thumb|center|alt text]]
+
[[File:AALSL.png|200px|thumb|center|alt text]]
<P>Pcons(J23101) + RBS(B0034) + PBAN(SL) + RBS(B0034) + BFP(K592100) + Ter(J61048)  
<P>Pcons(J23101) + RBS(B0034) + PBAN(SL) + RBS(B0034) + BFP(K592100) + Ter(J61048)  
This biobrick tests whether our biobrick work or not.
This biobrick tests whether our biobrick work or not.
Line 108: Line 108:
======PBAN Helicoverpa armigera Hubner 1======
======PBAN Helicoverpa armigera Hubner 1======
-
[[File:AA.png|200px|thumb|center|alt text]]
+
[[File:HAH.png|200px|thumb|center|alt text]]
Peptide Sequence:
Peptide Sequence:
LSDDMPARPADQEMYRQDPEQIDSRTKYFSPRL
LSDDMPARPADQEMYRQDPEQIDSRTKYFSPRL
Line 114: Line 114:
======PBAN Helicoverpa armigera Hubner 2======
======PBAN Helicoverpa armigera Hubner 2======
-
[[File:AA.png|200px|thumb|center|alt text]]
+
[[File:HALFHAH.png|200px|thumb|center|alt text]]
<P>Pcons(J23101) + RBS(B0034) + PBAN(HAH)  
<P>Pcons(J23101) + RBS(B0034) + PBAN(HAH)  
This biobrick produces Helicoverpa armigera Hubner’s PBAN.</P>
This biobrick produces Helicoverpa armigera Hubner’s PBAN.</P>
======PBAN Helicoverpa armigera Hubner 3======
======PBAN Helicoverpa armigera Hubner 3======
-
[[File:AA.png|200px|thumb|center|alt text]]
+
[[File:AALHAH.png|200px|thumb|center|alt text]]
<P>Pcons(J23101) + RBS(B0034) + PBAN(HAH) + RBS(B0034) + BFP(K592100) + Ter(J61048)  
<P>Pcons(J23101) + RBS(B0034) + PBAN(HAH) + RBS(B0034) + BFP(K592100) + Ter(J61048)  
This biobrick tests whether our biobrick work or not.
This biobrick tests whether our biobrick work or not.
Line 126: Line 126:
======PBAN Adoxophyes sp. 1======
======PBAN Adoxophyes sp. 1======
-
[[File:AA.png|200px|thumb|center|alt text]]
+
[[File:AS.png|200px|thumb|center|alt text]]
Peptide Sequence:
Peptide Sequence:
QSEAVTSSDEQVYRQDMSPVDGRLKYFSPRL
QSEAVTSSDEQVYRQDMSPVDGRLKYFSPRL
Line 132: Line 132:
======PBAN Adoxophyes sp. 2======
======PBAN Adoxophyes sp. 2======
-
[[File:AA.png|200px|thumb|center|alt text]]
+
[[File:HALFAS.png|200px|thumb|center|alt text]]
<P>Pcons(J23101) + RBS(B0034) + PBAN(AS)  
<P>Pcons(J23101) + RBS(B0034) + PBAN(AS)  
This biobrick produces Adoxophyes  sp’s PBAN.</P>
This biobrick produces Adoxophyes  sp’s PBAN.</P>
======PBAN Adoxophyes sp. 3======
======PBAN Adoxophyes sp. 3======
-
[[File:AA.png|200px|thumb|center|alt text]]
+
[[File:AALAS.png|200px|thumb|center|alt text]]
<P>Pcons(J23101) + RBS(B0034) + PBAN(AS) + RBS(B0034) + BFP(K592100) + Ter(J61048)  
<P>Pcons(J23101) + RBS(B0034) + PBAN(AS) + RBS(B0034) + BFP(K592100) + Ter(J61048)  
This biobrick tests whether our biobrick work or not.
This biobrick tests whether our biobrick work or not.
Line 144: Line 144:
======PBAN Solenopsis invicta 1======
======PBAN Solenopsis invicta 1======
-
[[File:AA.png|200px|thumb|center|alt text]]
+
[[File:SI.png|200px|thumb|center|alt text]]
Peptide Sequence:
Peptide Sequence:
GSGEDLSYGDAYEVDEDDHPLFVPR
GSGEDLSYGDAYEVDEDDHPLFVPR
Line 150: Line 150:
======PBAN Solenopsis invicta 2======
======PBAN Solenopsis invicta 2======
-
[[File:AA.png|200px|thumb|center|alt text]]
+
[[File:HALFSI.png|200px|thumb|center|alt text]]
<P>Pcons(J23101) + RBS(B0034) + PBAN(SI)  
<P>Pcons(J23101) + RBS(B0034) + PBAN(SI)  
This biobrick produces Solenopsis invicta’s PBAN.</P>
This biobrick produces Solenopsis invicta’s PBAN.</P>
======PBAN Solenopsis invicta 3======
======PBAN Solenopsis invicta 3======
-
[[File:AA.png|200px|thumb|center|alt text]]
+
[[File:ALLSI.png|200px|thumb|center|alt text]]
<P>Pcons(J23101) + RBS(B0034) + PBAN(SI) + RBS(B0034) + BFP(K592100) + Ter(J61048)  
<P>Pcons(J23101) + RBS(B0034) + PBAN(SI) + RBS(B0034) + BFP(K592100) + Ter(J61048)  
This biobrick tests whether our biobrick work or not.
This biobrick tests whether our biobrick work or not.
Line 168: Line 168:
======PBAN Aedes aegypti 2======
======PBAN Aedes aegypti 2======
-
[[File:AA.png|200px|thumb|center|alt text]]
+
[[File:HALFAA.png|200px|thumb|center|alt text]]
<P>Pcons(J23101) + RBS(B0034) + PBAN(AA)  
<P>Pcons(J23101) + RBS(B0034) + PBAN(AA)  
This biobrick produces Aedes aegypti’s PBAN.</P>
This biobrick produces Aedes aegypti’s PBAN.</P>
======PBAN Aedes aegypti 3======
======PBAN Aedes aegypti 3======
-
[[File:AA.png|200px|thumb|center|alt text]]
+
[[File:ALLAA.png|200px|thumb|center|alt text]]
<P>Pcons(J23101) + RBS(B0034) + PBAN(AA) + RBS(B0034) + BFP(K592100) + Ter(J61048)  
<P>Pcons(J23101) + RBS(B0034) + PBAN(AA) + RBS(B0034) + BFP(K592100) + Ter(J61048)  
This biobrick tests whether our biobrick work or not.
This biobrick tests whether our biobrick work or not.

Revision as of 17:25, 4 October 2014

Project

Parts submitted to the Registry

<groupparts>iGEM014 NCTU_Formosa</groupparts>

Brief Information

Please click on the name of the parts for detailed information that is hosted in the Registry website. Team:NCTU Formosa/file/test

PBAN-producted system

PBAN Bombyx mori 1
alt text

Peptide Sequence: LSEDMPATPADQEMYQPDPEEMESRTRYFSPRL

PBAN(BM)+pSB1C3 This biobrick is the PBAN on the pSB1C3 backbone.

PBAN Bombyx mori 2
alt text

Pcons(J23101) + RBS(B0034) + PBAN(BM) This biobrick produces Bombyx mori’s PBAN.

PBAN Bombyx mori 3
alt text

Pcons(J23101) + RBS(B0034) + PBAN(BM) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick work or not. If our biobrick works, it would glow blue light.


PBAN Mamestra brassicae 1
alt text

Peptide Sequence: LADDMPATPADQEMYRPDPEQIDSRTKYFSPRL

PBAN(MB)+pSB1C3 This biobrick is the PBAN on the pSB1C3 backbone.

PBAN Mamestra brassicae 2
alt text

Pcons(J23101) + RBS(B0034) + PBAN(MB) This biobrick produces Mamestra brassicae’s PBAN.

PBAN Mamestra brassicae 3
alt text

Pcons(J23101) + RBS(B0034) + PBAN(MB) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick work or not. If our biobrick works, it would glow blue light.


PBAN Agrotis ipsilon 1
alt text

Peptide Sequence: LADDTPATPADQEMYRPDPEQIDSRTKYFSPRL

PBAN(AI)+pSB1C3 This biobrick is the PBAN on the pSB1C3 backbone.

PBAN Agrotis ipsilon 2
alt text

Pcons(J23101) + RBS(B0034) + PBAN(AI) This biobrick produces Agrotis ipsilon’s PBAN.

PBAN Agrotis ipsilon 3
alt text

Pcons(J23101) + RBS(B0034) + PBAN(AI) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick work or not. If our biobrick works, it would glow blue light.


PBAN Lymantria dispar 1
alt text

Peptide Sequence: LADDMPATMADQEVYRPEPEQIDSRNKUFSPRL

PBAN(AI)+pSB1C3 This biobrick is the PBAN on the pSB1C3 backbone.

PBAN Lymantria dispar 2
alt text

Pcons(J23101) + RBS(B0034) + PBAN(LD) This biobrick produces Lymantria dispar’s PBAN.

PBAN Lymantria dispar 3
alt text

Pcons(J23101) + RBS(B0034) + PBAN(LD) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick work or not. If our biobrick works, it would glow blue light.


PBAN Spodoptera litura 1
alt text

Peptide Sequence: LADDMPATPADQELYRPDPDQIDSRTKUFSPRL

PBAN(SL)+pSB1C3 This biobrick is the PBAN on the pSB1C3 backbone.

PBAN Spodoptera litura 2
alt text

Pcons(J23101) + RBS(B0034) + PBAN(SL) This biobrick produces Spodoptera litura’s PBAN.

PBAN Spodoptera litura 3

Pcons(J23101) + RBS(B0034) + PBAN(SL) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick work or not. If our biobrick works, it would glow blue light.


PBAN Helicoverpa armigera Hubner 1
alt text

Peptide Sequence: LSDDMPARPADQEMYRQDPEQIDSRTKYFSPRL

PBAN(HAH)+pSB1C3 This biobrick is the PBAN on the pSB1C3 backbone.

PBAN Helicoverpa armigera Hubner 2
alt text

Pcons(J23101) + RBS(B0034) + PBAN(HAH) This biobrick produces Helicoverpa armigera Hubner’s PBAN.

PBAN Helicoverpa armigera Hubner 3

Pcons(J23101) + RBS(B0034) + PBAN(HAH) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick work or not. If our biobrick works, it would glow blue light.

PBAN Adoxophyes sp. 1
alt text

Peptide Sequence: QSEAVTSSDEQVYRQDMSPVDGRLKYFSPRL

PBAN(AS)+pSB1C3 This biobrick is the PBAN on the pSB1C3 backbone.

PBAN Adoxophyes sp. 2
alt text

Pcons(J23101) + RBS(B0034) + PBAN(AS) This biobrick produces Adoxophyes sp’s PBAN.

PBAN Adoxophyes sp. 3

Pcons(J23101) + RBS(B0034) + PBAN(AS) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick work or not. If our biobrick works, it would glow blue light.


PBAN Solenopsis invicta 1
alt text

Peptide Sequence: GSGEDLSYGDAYEVDEDDHPLFVPR

PBAN(SI)+pSB1C3 This biobrick is the PBAN on the pSB1C3 backbone.

PBAN Solenopsis invicta 2
alt text

Pcons(J23101) + RBS(B0034) + PBAN(SI) This biobrick produces Solenopsis invicta’s PBAN.

PBAN Solenopsis invicta 3
alt text

Pcons(J23101) + RBS(B0034) + PBAN(SI) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick work or not. If our biobrick works, it would glow blue light.


PBAN Aedes aegypti 1
alt text

Peptide Sequence: DASSSNENNSRPPFAPRL

PBAN(AA)+pSB1C3 This biobrick is the PBAN on the pSB1C3 backbone.

PBAN Aedes aegypti 2
alt text

Pcons(J23101) + RBS(B0034) + PBAN(AA) This biobrick produces Aedes aegypti’s PBAN.

PBAN Aedes aegypti 3
alt text

Pcons(J23101) + RBS(B0034) + PBAN(AA) + RBS(B0034) + BFP(K592100) + Ter(J61048) This biobrick tests whether our biobrick work or not. If our biobrick works, it would glow blue light.