Team:NCTU Formosa/biobricks

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__NOTOC__
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===Parts submitted to the Registry===
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===Parts Submitted to The Registry===
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Please click on the name of the parts for detailed information that is hosted in the Registry website.
<groupparts>iGEM014 NCTU_Formosa</groupparts>
<groupparts>iGEM014 NCTU_Formosa</groupparts>
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===Brief Information===
===Brief Information===
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Please click on the name of the parts for detailed information that is hosted in the Registry website.
 
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====Light-regulated system====
 
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======BBa_K1017726======
 
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*pcya and ho1 are the enzymes needed to convert heme into chromophore phycocyanobiline(PCB), the red light sensor. Since pcya and ho1 are not naturally produced in E.coli, we use P<sub>cons</sub> upstream in order to make E.coli continuously expressed them to synthesize PCB.
 
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<br>[[File:Nctu_pcya_ho1.jpg|450px]]
 
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======BBa_K1017301======
 
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*Cph8 is a chimeric light receptor. It is a fusion of the photoreceptor cph1 and the envZ histidine kinase. cph1 is only active when it binds the chromophore phycocyanobiline (PCB).
 
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<br>[[File:Nctu_cph8.jpg|150px]]
 
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======BBa_K1017781======
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=====PBAN ''Bombyx mori'' 1 (BBa_K1415001)=====
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*This is the key part to change Ompc promoter intro red promotor. The lacI will inhibit the function of P<sub>lac</sub>, thus, the original outcomes will be converted into our expected result.
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[[File:Pbanbm.png|176px|link=|frameless|left]]
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<br>[[File:Nctu_lacI_plac.jpg|300px]]
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Peptide Sequence:
 +
LSEDMPATPADQEMYQPDPEEMESRTRYFSPRL
 +
<P>PBAN(BM)+pSB1C3 This biobrick is the PBAN of ''Bombyx mori'' on the pSB1C3 backbone.</P>
 +
<h1></h1>
 +
=====PBAN ''Bombyx mori'' 2 (BBa_K1415101)=====
 +
[[File:HALFBM.png|457px|link=|frameless|left]]
 +
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(BM)
 +
This circuit is responsible for '''producing the ''Bombyx mori''’s PBAN'''. As ''Bombyx mori'' PBAN is ingested by the female ''Bombyx mori'', the female ''Bombyx mori'' will produce its own sex pheromone and attract male ''Bombyx moris''. </P>
 +
<h2></h2>
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======BBa_K1017101======
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=====PBAN ''Bombyx mori'' 3 (BBa_K1415201)=====
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*Ompc promtor, which can sense red light with the presence of cph8. It will be turned on in the dark ,and be turned off in the bright. For the convenient use, we add lacI and lac promotor downstream the biobrick. This part, so called red promotor, can be activated under red light, and inactive in the dark.  
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[[File:ALLBM.png|780px|link=|frameless|left]]
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<br>[[File:Nctu_Pred.jpg|450px]]
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<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(BM) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048)
 +
The above circuit is constructed mainly to test whether it produces ''Bombyx mori''’s PBAN.
 +
If the circuit do work, it would result in the expression of the BFP gene and emission of blue fluorescent light. </p>
 +
<h1></h1>
 +
=====PBAN ''Mamestra brassicae'' 1 (BBa_K1415002)=====
 +
[[File:MB.png|276px|link=|frameless|left]]
 +
Peptide Sequence:
 +
LADDMPATPADQEMYRPDPEQIDSRTKYFSPRL
 +
<P>PBAN(MB)+pSB1C3 This biobrick is the PBAN of ''Mamestra brassicae'' on the pSB1C3 backbone.</P>
 +
<h1></h1>
 +
=====''PBAN Mamestra brassicae'' 2 (BBa_K1415102)=====
 +
[[File:HALFMB.png|457px|link=|frameless|left]]
 +
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(MB)
 +
This circuit is responsible for '''producing the ''Mamestra brassicae’s'' PBAN'''. As ''Mamestra brassicae’s'' PBAN is ingested by the female ''Mamestra brassicae'', the female ''Mamestra brassicae'' will produce its own sex pheromone and attract male ''Mamestra brassicaes''. </P>
 +
<h1></h1>
 +
=====PBAN ''Mamestra brassicae'' 3 (BBa_K1415202)=====
 +
[[File:ALLMB.png|780px|link=|frameless|left]]
 +
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(MB) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048)
 +
The above circuit is constructed mainly to test whether it produces ''Mamestra brassicae''’s PBAN.
 +
If the circuit do work, it would result in the expression of the BFP gene and emission of blue fluorescent light.
 +
</P>
 +
<h1></h1>
 +
=====PBAN ''Agrotis ipsilon'' 1 (BBa_K1415003)=====
 +
[[File:AI.png|276px|link=|frameless|left]]
 +
Peptide Sequence:
 +
LADDTPATPADQEMYRPDPEQIDSRTKYFSPRL
 +
<P>PBAN(AI)+pSB1C3 This biobrick is the PBAN of ''Agrotis ipsilon'' on the pSB1C3 backbone.</P>
 +
<h1></h1>
 +
=====PBAN ''Agrotis ipsilon'' 2 (BBa_K1415103)=====
 +
[[File:HALFAI.png|457px|link=|frameless|left]]
 +
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(AI)
 +
This circuit is responsible for '''producing the ''Agrotis ipsilon''’s PBAN'''. As ''Agrotis ipsilon''’s PBAN is ingested by the female ''Agrotis ipsilon'', the female ''Agrotis ipsilon'' will produce its own sex pheromone and attract male ''Agrotis ipsilons''.</P>
 +
<h1></h1>
 +
=====PBAN ''Agrotis ipsilon'' 3 (BBa_K1415203)=====
 +
[[File:ALLAI.png|780px|link=|frameless|left]]
 +
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(AI) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048)
 +
The above circuit is constructed mainly to test whether it produces ''Agrotis ipsilon''’s PBAN.
 +
If the circuit do work, it would result in the expression of the BFP gene and emission of blue fluorescent light.
 +
</P>
 +
<h1></h1>
 +
=====PBAN ''Lymantria dispar'' 1 (BBa_K1415004)=====
 +
[[File:LD.png|276px|link=|frameless|left]]
 +
Peptide Sequence:
 +
LADDMPATMADQEVYRPEPEQIDSRNKUFSPRL
 +
<P>PBAN(AI)+pSB1C3 This biobrick is the PBAN of ''Lymantria dispar'' on the pSB1C3 backbone.</P>
 +
<h1></h1>
 +
=====PBAN ''Lymantria dispar'' 2 (BBa_K1415104)=====
 +
[[File:HALFLD.png|457px|link=|frameless|left]]
 +
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(LD)
 +
This circuit is responsible for''' producing ''Lymantria dispar''’s PBAN'''. As ''Lymantria dispar'' PBAN is ingested by the female ''Lymantria dispar'', the female ''Lymantria dispar'' will produce its own sex pheromone and attract male ''Lymantria dispars''.</P>
 +
<h1></h1>
 +
=====PBAN ''Lymantria dispar'' 3 (BBa_K1415204)=====
 +
[[File:ALLLD.png|780px|link=|frameless|left]]
 +
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(LD) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048)
 +
The above circuit is constructed mainly to test whether it produces ''Lymantria dispar''’s PBAN.
 +
If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.
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====Temperature-regulated system====
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</P>
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======BBa_K1017602======
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<h1></h1>
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*By using mGFP as a reporter gene, we can test whether the 37 °C RBS works.
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=====PBAN ''Spodoptera litura'' 1 (BBa_K1415005)=====
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<br>[[File:Nctu_37rbs_mGFP.jpg|200px]]
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[[File:SL.png|276px|link=|frameless|left]]
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Peptide Sequence:
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======BBa_K1017603======
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LADDMPATPADQELYRPDPDQIDSRTKUFSPRL
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*In our circuit, this biobrick is the part of P<sub>lux</sub>'s activation when the temperature reaches to 37<sup>o</sup>C.
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<P>PBAN(SL)+pSB1C3 This biobrick is the PBAN of ''Spodoptera litura'' on the pSB1C3 backbone.</P>
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<br>
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<h1></h1>
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[[File:Nctu_37rbs_luxr.jpg|200px]]
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=====PBAN ''Spodoptera litura'' 2 (BBa_K1415105)=====
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[[File:HALFSL.png|457px|link=|frameless|left]]
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====Small RNA-regulated system====
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<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(SL)
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+
This circuit is responsible for '''producing the ''Spodoptera litura''’s PBAN'''. As ''Spodoptera litura'' PBAN is ingested by the female ''Spodoptera litura'', the female ''Spodoptera litura'' will produce its own sex pheromone and attract male ''Spodoptera lituras''.</P>
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======BBa_K1017403======
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<h1></h1>
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*The sRNA is the complement of its rRBS. It can regulate the downstream of rRBS in RNA level by binding onto the rRBS when it is transcribed in order to interrupt ribosomes' work. In addition, adding Plux upstream makes the sequence be controlled by luxR/AHL complex.<br>
+
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[[File:Nctu_plux_srna1.jpg|250px]]
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-
 
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======BBa_K1017404======
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*The sRNA is the complement of its rRBS. It can regulate the downstream of rRBS in RNA level by binding onto the rRBS when it is transcribed in order to interrupt ribosomes' work.<br>[[File:Nctu_srna2.jpg|200px]]
+
-
 
+
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======BBa_K1017202======
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*The sRNA, base pair with target mRNA, including the Shine-Dalgarno sequence. Thus it prevent ribosome from binding to initiate the translation. The rRBS is designed for sRNA perfect binding, and this rRBS is the RBS which can be bound only for our artificial sRNA(BBa_K1017404).<br>
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[[File:Nctu_rbs2.jpg|150px]]
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======BBa_K1017811======
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*The sequence let us show the efficiency of the sRNA-rRBS binding by expressing the fluorescence with the combination of a sequence providing the transcription of the sRNA.
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[[File:Nctu_pcons_rbs2_mrfp.jpg|350px]]
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======BBa_K1017401======
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*This part, BBa_K1017401, includes our artificial sRNA-1 and rRBS-1. The non-coding small RNA can bind to the Shine-Dalgarno sequence on rRBS-1 by base-pairing. Once the rRBS-1 is blocked, ribosomes cannot bind to it to translate, thus, gene expressions downstream are decreased. Because of specific binding, rRBS-1 can only be bound by sRNA-1. We add P<sub>lux</sub> upstream, so this part can be regulated by luxR/AHL  complex. There is one important thing hasn't be mentioned is that it is a temporary sequence which contains the restriction enzyme cutting sites of SpeI, EcoRI and XbaI in order for us to separate them.<br>
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[[File:Nctu_sRNA_rRBS1.jpg|350px]]
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-
 
+
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======BBa_K1017402======
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*This part, BBa_K1017402, is similar to BBa_K1017401 mentioned above, but without P<sub>lux</sub>. rRBS-2 can only be bound by sRNA-2 due to specificity, then gene expressions downstream are decreased. There is one important thing hasn't be mentioned is that it is a temporary sequence which contains the restriction enzyme cutting sites of SpeI, EcoRI and XbaI in order for us to separate them.<br>
+
-
[[File:Nctu_srna2_rRNS.jpg|250px]]
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 +
=====PBAN ''Spodoptera litura'' 3 (BBa_K1415205)=====
 +
[[File:ALLSL.png|780px|link=|frameless|left]]
 +
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(SL) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048)
 +
The above circuit is constructed mainly to test whether it produces ''Spodoptera litura''’s PBAN.
 +
If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.
 +
</P>
 +
<h1></h1>
 +
=====PBAN ''Helicoverpa armigera Hubner'' 1 (BBa_K1415006)=====
 +
[[File:HAH.png|276px|link=|frameless|left]]
 +
Peptide Sequence:
 +
LSDDMPARPADQEMYRQDPEQIDSRTKYFSPRL
 +
<P>PBAN(HAH)+pSB1C3 This biobrick is the PBAN of ''Helicoverpa armigera Hubner'' on the pSB1C3 backbone.</P>
 +
<h1></h1>
 +
=====PBAN ''Helicoverpa armigera Hubner'' 2 (BBa_K1415106)=====
 +
[[File:HALFHAH.png|457px|link=|frameless|left]]
 +
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(HAH)
 +
This circuit is responsible for''' producing the ''Helicoverpa armigera Hubner''’s PBAN'''. As ''Helicoverpa armigera Hubner''’s PBAN is ingested by the female ''Helicoverpa armigera Hubner'', the female ''Helicoverpa armigera Hubner'' will produce its own sex pheromone and attract male ''Helicoverpa armigera Hubner''s.</P>
 +
<h1></h1>
 +
=====PBAN ''Helicoverpa armigera Hubner'' 3 (BBa_K1415206)=====
 +
[[File:ALLHAH.png|780px|link=|frameless|left]]
 +
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(HAH) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048)
 +
The above circuit is constructed mainly to test whether it produces ''Helicoverpa armigera Hubner''’s PBAN.
 +
If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.
 +
</P>
 +
<h1></h1>
 +
=====PBAN ''Adoxophyes sp.'' 1 (BBa_K1415007)=====
 +
[[File:AS.png|276px|link=|frameless|left]]
 +
Peptide Sequence:
 +
QSEAVTSSDEQVYRQDMSPVDGRLKYFSPRL
 +
<P>PBAN(AS)+pSB1C3 This biobrick is the PBAN of ''Adoxophyes sp.'' on the pSB1C3 backbone.</P>
 +
<h1></h1>
 +
=====PBAN ''Adoxophyes sp.'' 2 (BBa_K1415107)=====
 +
[[File:HALFAS.png|457px|link=|frameless|left]]
 +
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(AS)
 +
This circuit is responsible for '''producing the ''Adoxophyes sp.''’s PBAN'''. As ''Adoxophyes sp.''’s PBAN is ingested by the female ''Adoxophyes sp.'', the female ''Adoxophyes sp.'' will produce its own sex pheromone and attract male ''Adoxophyes sp.''</P>
 +
<h1></h1>
 +
=====PBAN ''Adoxophyes sp.'' 3 (BBa_K1415207)=====
 +
[[File:ALLAS.png|780px|link=|frameless|left]]
 +
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(AS) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048)
 +
The above circuit is constructed mainly to test whether it produces ''Adoxophyes sp.''’s PBAN.
 +
If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.
 +
</P>
 +
<h1></h1>
 +
=====PBAN ''Solenopsis invicta'' 1 (BBa_K1415008)=====
 +
[[File:SI.png|276px|link=|frameless|left]]
 +
Peptide Sequence:
 +
GSGEDLSYGDAYEVDEDDHPLFVPR
 +
<P>PBAN(SI)+pSB1C3 This biobrick is the PBAN of ''Solenopsis invicta'' on the pSB1C3 backbone.</P>
 +
<h1></h1>
 +
=====PBAN ''Solenopsis invicta'' 2 (BBa_K1415108)=====
 +
[[File:HALFSI.png|457px|link=|frameless|left]]
 +
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(SI)
 +
This circuit is responsible for '''producing the ''Solenopsis invicta''’s PBAN'''. As ''Solenopsis invicta''’s PBAN is ingested by the female ''Solenopsis invicta'', the female ''Solenopsis invicta'' will produce its own sex pheromone and attract male ''Solenopsis invicta''s.</P>
 +
<h1></h1>
 +
=====PBAN ''Solenopsis invicta'' 3 (BBa_K1415208)=====
 +
[[File:ALLSI.png|780px|link=|frameless|left]]
 +
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(SI) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048)
 +
The above circuit is constructed mainly to test whether it produces ''Solenopsis invicta'' PBAN.
 +
If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.
 +
</P>
 +
<h1></h1>
 +
=====PBAN ''Aedes aegypti'' 1 (BBa_K1415009)=====
 +
[[File:AA.png|276px|link=|frameless|left]]
 +
Peptide Sequence:
 +
DASSSNENNSRPPFAPRL
 +
<P>PBAN(AA)+pSB1C3 This biobrick is the PBAN of ''Aedes aegypti'' on the pSB1C3 backbone.</P>
 +
<h1></h1>
 +
=====PBAN ''Aedes aegypti'' 2 (BBa_K1415109)=====
 +
[[File:HALFAA.png|457px|link=|frameless|left]]
 +
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(AA)
 +
This circuit is responsible for '''producing the ''Aedes aegypti''’s PBAN'''. As ''Aedes aegypti''’s PBAN is ingested by the female ''Aedes aegypti'', the female ''Aedes aegypti'' will produce its own sex pheromone and attract male ''Aedes aegyptis''.</P>
 +
<h1></h1>
 +
=====PBAN ''Aedes aegypti'' 3 (BBa_K1415209)=====
 +
[[File:ALLAA.png|780px|link=|frameless|left]]
 +
<P>P<sub>cons</sub>(BBa_J23101) + RBS(BBa_B0034) + PBAN(AA) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048)
 +
The above circuit is constructed mainly to test whether it produces ''Aedes aegypti''’s PBAN.
 +
If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.</p>
 +
<h1></h1>
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<div id="footer-wrapper">
 
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  <div id="footer"> <div id="footer-text">
 
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    <p>2013 NCTU_Formosa</p>
 
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    <p class="author">Website designed by Calvin Hue.</p>
 
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    <p>Cover image credit: <a href="http://www.dvq.co.nz/" target="_blank">DVQ</a></p>
 
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    </div> </div>
 
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Latest revision as of 02:25, 18 October 2014

Project

Change the font size right here

Parts Submitted to The Registry

Please click on the name of the parts for detailed information that is hosted in the Registry website. <groupparts>iGEM014 NCTU_Formosa</groupparts>

Brief Information

PBAN Bombyx mori 1 (BBa_K1415001)
Pbanbm.png

Peptide Sequence: LSEDMPATPADQEMYQPDPEEMESRTRYFSPRL

PBAN(BM)+pSB1C3 This biobrick is the PBAN of Bombyx mori on the pSB1C3 backbone.

PBAN Bombyx mori 2 (BBa_K1415101)
HALFBM.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(BM) This circuit is responsible for producing the Bombyx mori’s PBAN. As Bombyx mori PBAN is ingested by the female Bombyx mori, the female Bombyx mori will produce its own sex pheromone and attract male Bombyx moris.

PBAN Bombyx mori 3 (BBa_K1415201)
ALLBM.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(BM) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Bombyx mori’s PBAN. If the circuit do work, it would result in the expression of the BFP gene and emission of blue fluorescent light.

PBAN Mamestra brassicae 1 (BBa_K1415002)
MB.png

Peptide Sequence: LADDMPATPADQEMYRPDPEQIDSRTKYFSPRL

PBAN(MB)+pSB1C3 This biobrick is the PBAN of Mamestra brassicae on the pSB1C3 backbone.

PBAN Mamestra brassicae 2 (BBa_K1415102)
HALFMB.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(MB) This circuit is responsible for producing the Mamestra brassicae’s PBAN. As Mamestra brassicae’s PBAN is ingested by the female Mamestra brassicae, the female Mamestra brassicae will produce its own sex pheromone and attract male Mamestra brassicaes.

PBAN Mamestra brassicae 3 (BBa_K1415202)
ALLMB.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(MB) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Mamestra brassicae’s PBAN. If the circuit do work, it would result in the expression of the BFP gene and emission of blue fluorescent light.

PBAN Agrotis ipsilon 1 (BBa_K1415003)
AI.png

Peptide Sequence: LADDTPATPADQEMYRPDPEQIDSRTKYFSPRL

PBAN(AI)+pSB1C3 This biobrick is the PBAN of Agrotis ipsilon on the pSB1C3 backbone.

PBAN Agrotis ipsilon 2 (BBa_K1415103)
HALFAI.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(AI) This circuit is responsible for producing the Agrotis ipsilon’s PBAN. As Agrotis ipsilon’s PBAN is ingested by the female Agrotis ipsilon, the female Agrotis ipsilon will produce its own sex pheromone and attract male Agrotis ipsilons.

PBAN Agrotis ipsilon 3 (BBa_K1415203)
ALLAI.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(AI) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Agrotis ipsilon’s PBAN. If the circuit do work, it would result in the expression of the BFP gene and emission of blue fluorescent light.

PBAN Lymantria dispar 1 (BBa_K1415004)
LD.png

Peptide Sequence: LADDMPATMADQEVYRPEPEQIDSRNKUFSPRL

PBAN(AI)+pSB1C3 This biobrick is the PBAN of Lymantria dispar on the pSB1C3 backbone.

PBAN Lymantria dispar 2 (BBa_K1415104)
HALFLD.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(LD) This circuit is responsible for producing Lymantria dispar’s PBAN. As Lymantria dispar PBAN is ingested by the female Lymantria dispar, the female Lymantria dispar will produce its own sex pheromone and attract male Lymantria dispars.

PBAN Lymantria dispar 3 (BBa_K1415204)
ALLLD.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(LD) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Lymantria dispar’s PBAN. If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.

PBAN Spodoptera litura 1 (BBa_K1415005)
SL.png

Peptide Sequence: LADDMPATPADQELYRPDPDQIDSRTKUFSPRL

PBAN(SL)+pSB1C3 This biobrick is the PBAN of Spodoptera litura on the pSB1C3 backbone.

PBAN Spodoptera litura 2 (BBa_K1415105)
HALFSL.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(SL) This circuit is responsible for producing the Spodoptera litura’s PBAN. As Spodoptera litura PBAN is ingested by the female Spodoptera litura, the female Spodoptera litura will produce its own sex pheromone and attract male Spodoptera lituras.

PBAN Spodoptera litura 3 (BBa_K1415205)
ALLSL.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(SL) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Spodoptera litura’s PBAN. If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.

PBAN Helicoverpa armigera Hubner 1 (BBa_K1415006)
HAH.png

Peptide Sequence: LSDDMPARPADQEMYRQDPEQIDSRTKYFSPRL

PBAN(HAH)+pSB1C3 This biobrick is the PBAN of Helicoverpa armigera Hubner on the pSB1C3 backbone.

PBAN Helicoverpa armigera Hubner 2 (BBa_K1415106)
HALFHAH.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(HAH) This circuit is responsible for producing the Helicoverpa armigera Hubner’s PBAN. As Helicoverpa armigera Hubner’s PBAN is ingested by the female Helicoverpa armigera Hubner, the female Helicoverpa armigera Hubner will produce its own sex pheromone and attract male Helicoverpa armigera Hubners.

PBAN Helicoverpa armigera Hubner 3 (BBa_K1415206)
ALLHAH.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(HAH) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Helicoverpa armigera Hubner’s PBAN. If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.

PBAN Adoxophyes sp. 1 (BBa_K1415007)
AS.png

Peptide Sequence: QSEAVTSSDEQVYRQDMSPVDGRLKYFSPRL

PBAN(AS)+pSB1C3 This biobrick is the PBAN of Adoxophyes sp. on the pSB1C3 backbone.

PBAN Adoxophyes sp. 2 (BBa_K1415107)
HALFAS.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(AS) This circuit is responsible for producing the Adoxophyes sp.’s PBAN. As Adoxophyes sp.’s PBAN is ingested by the female Adoxophyes sp., the female Adoxophyes sp. will produce its own sex pheromone and attract male Adoxophyes sp.

PBAN Adoxophyes sp. 3 (BBa_K1415207)
ALLAS.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(AS) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Adoxophyes sp.’s PBAN. If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.

PBAN Solenopsis invicta 1 (BBa_K1415008)
SI.png

Peptide Sequence: GSGEDLSYGDAYEVDEDDHPLFVPR

PBAN(SI)+pSB1C3 This biobrick is the PBAN of Solenopsis invicta on the pSB1C3 backbone.

PBAN Solenopsis invicta 2 (BBa_K1415108)
HALFSI.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(SI) This circuit is responsible for producing the Solenopsis invicta’s PBAN. As Solenopsis invicta’s PBAN is ingested by the female Solenopsis invicta, the female Solenopsis invicta will produce its own sex pheromone and attract male Solenopsis invictas.

PBAN Solenopsis invicta 3 (BBa_K1415208)
ALLSI.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(SI) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Solenopsis invicta PBAN. If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.

PBAN Aedes aegypti 1 (BBa_K1415009)
AA.png

Peptide Sequence: DASSSNENNSRPPFAPRL

PBAN(AA)+pSB1C3 This biobrick is the PBAN of Aedes aegypti on the pSB1C3 backbone.

PBAN Aedes aegypti 2 (BBa_K1415109)
HALFAA.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(AA) This circuit is responsible for producing the Aedes aegypti’s PBAN. As Aedes aegypti’s PBAN is ingested by the female Aedes aegypti, the female Aedes aegypti will produce its own sex pheromone and attract male Aedes aegyptis.

PBAN Aedes aegypti 3 (BBa_K1415209)
ALLAA.png

Pcons(BBa_J23101) + RBS(BBa_B0034) + PBAN(AA) + RBS(BBa_B0034) + BFP(BBa_K592100)+ Ter(BBa_J61048) The above circuit is constructed mainly to test whether it produces Aedes aegypti’s PBAN. If the circuit do work, it would result in the expression of the BFP gene and the emission of blue fluorescent light.

Retrieved from "http://2014.igem.org/Team:NCTU_Formosa/biobricks"