Team:NCTU Formosa/Notebook/test7

From 2014.igem.org

(Difference between revisions)
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6. Pcons+RBS+SacI-11+RBS+BFP+TER
6. Pcons+RBS+SacI-11+RBS+BFP+TER
7. Pcons+RBS+ Nhel-20+RBS+BFP+TER
7. Pcons+RBS+ Nhel-20+RBS+BFP+TER
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</p>
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<p>
Ligation
Ligation
Member:Mick
Member:Mick
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</p><p>
</p><p>
2014/10/9
2014/10/9
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</p><p>
Cultivation at 12:00
Cultivation at 12:00
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</p>
ALL Nhel-9(10/7)x2
ALL Nhel-9(10/7)x2
ALL SacI-11(10/7)x1
ALL SacI-11(10/7)x1
ALL xhol-2(10/7)x1
ALL xhol-2(10/7)x1
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<p>
Electrophoresis
Electrophoresis
The first piece of gel
The first piece of gel
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</p>
1. maker
1. maker
2. Pcons+RBS+NheI-20+RBS+BFP+TER
2. Pcons+RBS+NheI-20+RBS+BFP+TER
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16. Pcons+RBS+SacI-11
16. Pcons+RBS+SacI-11
17. Maker
17. Maker
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<p>
The second piece of gel
The second piece of gel
Member: Chris Ho
Member: Chris Ho
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</p><p>
1. maker
1. maker
2. Pcons+RBS+NheI-11
2. Pcons+RBS+NheI-11
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6. Pcons+RBS+SalI-12
6. Pcons+RBS+SalI-12
7. Pcons+RBS+Xhol
7. Pcons+RBS+Xhol
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</p><p>
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2. Pcons+RBS+KpnI-1 to psb3c5
2. Pcons+RBS+KpnI-1 to psb3c5
3. Pcons+RBS+NcoI-1 to psb3c5
3. Pcons+RBS+NcoI-1 to psb3c5
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</p><p>
Mini-prep and sequencing
Mini-prep and sequencing
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Pcons+RBS+Ndel on psb3c5
Pcons+RBS+Ndel on psb3c5
Pcons+RBS+NcoI on psb3c5
Pcons+RBS+NcoI on psb3c5
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</p><p>
Electrophoresis
Electrophoresis
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4. Pcons+RBS+NcoI-1 on psb3c5
4. Pcons+RBS+NcoI-1 on psb3c5
</p>
</p>
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<br></div>
<br></div>

Revision as of 11:51, 13 October 2014

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1

2014/10/8 Sequencing 1. PBAN NcoI 2. Pcons+RBS+SalI -1 3. Pcons+RBS+SalI-11 4. Pcons+RBS+NheI-6 5. Pcons+RBS+xhol-2+RBS+BFP+TER 6. Pcons+RBS+SacI-11+RBS+BFP+TER 7. Pcons+RBS+ Nhel-20+RBS+BFP+TER

Ligation Member:Mick Pcons+RBS+NcoI on psb3c5 Pcons+RBS+NcoI on psb3c5

2014/10/9

Cultivation at 12:00

ALL Nhel-9(10/7)x2 ALL SacI-11(10/7)x1 ALL xhol-2(10/7)x1

Electrophoresis The first piece of gel

1. maker 2. Pcons+RBS+NheI-20+RBS+BFP+TER 3. Pcons+RBS+Hind-1+RBS+BFP+TER 4. Pcons+RBS+KpnI-1+RBS+BFP+TER 5. Pcons+RBS+Ndel-1+RBS+BFP+TER 6. Pcons+RBS+NcoI-5+RBS+BFP+TER 7. Pcons+RBS+AseI ALL 8. PBAN NcoI-12 9. Pcons+RBS+NheI-6 10. Pcons+RBS+Hind-1 11. Pcons+RBS+NcoI-20 12. Pcons+RBS+NcoI-2-2 13. Pcons+RBS+Ndel-2-1 14. Pcons+RBS+Nedl-2-2 15. Pcons+RBS+SacI-1 16. Pcons+RBS+SacI-11 17. Maker

The second piece of gel Member: Chris Ho

1. maker 2. Pcons+RBS+NheI-11 3. Pcons+RBS+Hind3 4. Pcons+RBS+KpnI-7 5. Pcons+RBS+SacI-16 6. Pcons+RBS+SalI-12 7. Pcons+RBS+Xhol

Start at 2:15 1 .Pcons+RBS+SacI-3 to psb3c5 2. Pcons+RBS+KpnI-1 to psb3c5 3. Pcons+RBS+NcoI-1 to psb3c5

Mini-prep and sequencing 1. ALL NheI-2 x1 2. ALL SacI-11 x1 3. ALL Xhol-2 X1

2014/10/10 Transform Start at 8:45 Pcons+RBS+Ndel on psb3c5 Pcons+RBS+NcoI on psb3c5

Electrophoresis 1. maker 2. Pcons+RBS+SacI-3 on psb3c5 3. Pcons+RBS+KpnI-1 on psb3c5 4. Pcons+RBS+NcoI-1 on psb3c5


2014/10/10 Transform Start at 8:45 Pcons+RBS+Ndel on psb3c5 Pcons+RBS+NcoI on psb3c5 Electrophoresis 1. maker 2. Pcons+RBS+SacI-3 on psb3c5 3. Pcons+RBS+KpnI-1 on psb3c5 4. Pcons+RBS+NcoI-1 on psb3c5

2014/10/12 PCR and Electrophoresis 1. Pcons+RBS+NcoI+psb3c5 2. Pcons+RBS+Ndel+psb3c5


4
5

Retrieved from "http://2014.igem.org/Team:NCTU_Formosa/Notebook/test7"