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Team:NCTU Formosa/Notebook/test7
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<div class="single note">1 | <div class="single note">1 | ||
<br></div> | <br></div> | ||
| - | <div class="single note">2 | + | <div class="single note"><p>2014/10/8 |
| + | Sequencing | ||
| + | 1. PBAN NcoI | ||
| + | 2. Pcons+RBS+SalI -1 | ||
| + | 3. Pcons+RBS+SalI-11 | ||
| + | 4. Pcons+RBS+NheI-6 | ||
| + | 5. Pcons+RBS+xhol-2+RBS+BFP+TER | ||
| + | 6. Pcons+RBS+SacI-11+RBS+BFP+TER | ||
| + | 7. Pcons+RBS+ Nhel-20+RBS+BFP+TER | ||
| + | |||
| + | Ligation | ||
| + | Member:Mick | ||
| + | Pcons+RBS+NcoI on psb3c5 | ||
| + | Pcons+RBS+NcoI on psb3c5 | ||
| + | </p><p> | ||
| + | 2014/10/9 | ||
| + | |||
| + | Cultivation at 12:00 | ||
| + | ALL Nhel-9(10/7)x2 | ||
| + | ALL SacI-11(10/7)x1 | ||
| + | ALL xhol-2(10/7)x1 | ||
| + | |||
| + | Electrophoresis | ||
| + | The first piece of gel | ||
| + | 1. maker | ||
| + | 2. Pcons+RBS+NheI-20+RBS+BFP+TER | ||
| + | 3. Pcons+RBS+Hind-1+RBS+BFP+TER | ||
| + | 4. Pcons+RBS+KpnI-1+RBS+BFP+TER | ||
| + | 5. Pcons+RBS+Ndel-1+RBS+BFP+TER | ||
| + | 6. Pcons+RBS+NcoI-5+RBS+BFP+TER | ||
| + | 7. Pcons+RBS+AseI ALL | ||
| + | 8. PBAN NcoI-12 | ||
| + | 9. Pcons+RBS+NheI-6 | ||
| + | 10. Pcons+RBS+Hind-1 | ||
| + | 11. Pcons+RBS+NcoI-20 | ||
| + | 12. Pcons+RBS+NcoI-2-2 | ||
| + | 13. Pcons+RBS+Ndel-2-1 | ||
| + | 14. Pcons+RBS+Nedl-2-2 | ||
| + | 15. Pcons+RBS+SacI-1 | ||
| + | 16. Pcons+RBS+SacI-11 | ||
| + | 17. Maker | ||
| + | |||
| + | The second piece of gel | ||
| + | Member: Chris Ho | ||
| + | 1. maker | ||
| + | 2. Pcons+RBS+NheI-11 | ||
| + | 3. Pcons+RBS+Hind3 | ||
| + | 4. Pcons+RBS+KpnI-7 | ||
| + | 5. Pcons+RBS+SacI-16 | ||
| + | 6. Pcons+RBS+SalI-12 | ||
| + | 7. Pcons+RBS+Xhol | ||
| + | |||
| + | |||
| + | Start at 2:15 | ||
| + | 1 .Pcons+RBS+SacI-3 to psb3c5 | ||
| + | 2. Pcons+RBS+KpnI-1 to psb3c5 | ||
| + | 3. Pcons+RBS+NcoI-1 to psb3c5 | ||
| + | |||
| + | Mini-prep and sequencing | ||
| + | 1. ALL NheI-2 x1 | ||
| + | 2. ALL SacI-11 x1 | ||
| + | 3. ALL Xhol-2 X1 | ||
| + | </p><p> | ||
| + | 2014/10/10 | ||
| + | Transform | ||
| + | Start at 8:45 | ||
| + | Pcons+RBS+Ndel on psb3c5 | ||
| + | Pcons+RBS+NcoI on psb3c5 | ||
| + | |||
| + | Electrophoresis | ||
| + | |||
| + | 1. maker | ||
| + | 2. Pcons+RBS+SacI-3 on psb3c5 | ||
| + | 3. Pcons+RBS+KpnI-1 on psb3c5 | ||
| + | 4. Pcons+RBS+NcoI-1 on psb3c5 | ||
| + | </p> | ||
| + | |||
<br></div> | <br></div> | ||
| - | <div class="single note">3 | + | <div class="single note"><p> |
| + | 2014/10/10 | ||
| + | Transform | ||
| + | Start at 8:45 | ||
| + | Pcons+RBS+Ndel on psb3c5 | ||
| + | Pcons+RBS+NcoI on psb3c5 | ||
| + | |||
| + | Electrophoresis | ||
| + | |||
| + | 1. maker | ||
| + | 2. Pcons+RBS+SacI-3 on psb3c5 | ||
| + | 3. Pcons+RBS+KpnI-1 on psb3c5 | ||
| + | 4. Pcons+RBS+NcoI-1 on psb3c5 | ||
| + | </p><p> | ||
| + | 2014/10/12 | ||
| + | PCR and Electrophoresis | ||
| + | |||
| + | 1. Pcons+RBS+NcoI+psb3c5 | ||
| + | 2. Pcons+RBS+Ndel+psb3c5 | ||
| + | </p> | ||
<br></div> | <br></div> | ||
<div class="single note">4 | <div class="single note">4 | ||
Revision as of 11:46, 13 October 2014
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2014/10/8 Sequencing 1. PBAN NcoI 2. Pcons+RBS+SalI -1 3. Pcons+RBS+SalI-11 4. Pcons+RBS+NheI-6 5. Pcons+RBS+xhol-2+RBS+BFP+TER 6. Pcons+RBS+SacI-11+RBS+BFP+TER 7. Pcons+RBS+ Nhel-20+RBS+BFP+TER Ligation Member:Mick Pcons+RBS+NcoI on psb3c5 Pcons+RBS+NcoI on psb3c5
2014/10/9 Cultivation at 12:00 ALL Nhel-9(10/7)x2 ALL SacI-11(10/7)x1 ALL xhol-2(10/7)x1 Electrophoresis The first piece of gel 1. maker 2. Pcons+RBS+NheI-20+RBS+BFP+TER 3. Pcons+RBS+Hind-1+RBS+BFP+TER 4. Pcons+RBS+KpnI-1+RBS+BFP+TER 5. Pcons+RBS+Ndel-1+RBS+BFP+TER 6. Pcons+RBS+NcoI-5+RBS+BFP+TER 7. Pcons+RBS+AseI ALL 8. PBAN NcoI-12 9. Pcons+RBS+NheI-6 10. Pcons+RBS+Hind-1 11. Pcons+RBS+NcoI-20 12. Pcons+RBS+NcoI-2-2 13. Pcons+RBS+Ndel-2-1 14. Pcons+RBS+Nedl-2-2 15. Pcons+RBS+SacI-1 16. Pcons+RBS+SacI-11 17. Maker The second piece of gel Member: Chris Ho 1. maker 2. Pcons+RBS+NheI-11 3. Pcons+RBS+Hind3 4. Pcons+RBS+KpnI-7 5. Pcons+RBS+SacI-16 6. Pcons+RBS+SalI-12 7. Pcons+RBS+Xhol Start at 2:15 1 .Pcons+RBS+SacI-3 to psb3c5 2. Pcons+RBS+KpnI-1 to psb3c5 3. Pcons+RBS+NcoI-1 to psb3c5 Mini-prep and sequencing 1. ALL NheI-2 x1 2. ALL SacI-11 x1 3. ALL Xhol-2 X1
2014/10/10 Transform Start at 8:45 Pcons+RBS+Ndel on psb3c5 Pcons+RBS+NcoI on psb3c5 Electrophoresis 1. maker 2. Pcons+RBS+SacI-3 on psb3c5 3. Pcons+RBS+KpnI-1 on psb3c5 4. Pcons+RBS+NcoI-1 on psb3c5
2014/10/10 Transform Start at 8:45 Pcons+RBS+Ndel on psb3c5 Pcons+RBS+NcoI on psb3c5 Electrophoresis 1. maker 2. Pcons+RBS+SacI-3 on psb3c5 3. Pcons+RBS+KpnI-1 on psb3c5 4. Pcons+RBS+NcoI-1 on psb3c5
2014/10/12 PCR and Electrophoresis 1. Pcons+RBS+NcoI+psb3c5 2. Pcons+RBS+Ndel+psb3c5
