Team:NCTU Formosa/Notebook/test7

From 2014.igem.org

(Difference between revisions)

Latest revision as of 15:23, 17 October 2014

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10/3

Cultivation of

[P_cons+RBS+NdeI]Cr

[P_cons+RBS+NcoI]Cr

[KpnI-1]Cr

[NdeI-1]Cr

[XhoI-1]Cr

[SacI-1]Cr

[AseI-1]Cr

[P_cons+RBS+HindIII-1]Cr

[P_cons+RBS+KpnI-4]Cr

[P_cons+RBS+AseI-5]Cr

[P_cons+RBS+XhoI-1]Cr

[P_cons+RBS+SacI-1]Cr

[P_cons+RBS+KpnI-1+J61048]Cr

[SalI-4]Cr

[P_cons+RBS+SalI-3+J61048]Cr

[HindIII-4]Cr

[P_cons+RBS+AseI+J61048]Cr

[NheI]

[NcoI]

Electrophoresis of

[NdeI](EP)

[NcoI](EP)

[SacI](EP)

Transformation of

[P_cons+RBS+NdeI]Cr

[P_cons+RBS+SacI]Cr

DNA Sequencing of

[P_cons+RBS+NdeI+J61048-1]

[P_cons+RBS+HindIII+J61048-1]

[P_cons+RBS+SalI+J61048-4]

[P_cons+RBS+SacI+J61048-1]

[P_cons+RBS+SacI+J61048-1](fail)

10/4

Digestion of

[P_cons+RBS+XhoI+J61048-2, -4, -5]

[P_cons+RBS+SacI+J61048-10, -11]

[P_cons+RBS+NheI+J61048-20]

[P_cons+RBS+NheI]Cr

[P_cons+RBS+NcoI]Cr

Cultivation of

[P_cons+RBS+NheI-1, -2, -4, -6, -10, -12]

[P_cons+RBS+SalI-1, -3, -6, -7, -11, -12]

Cultivation of

[P_cons+RBS+NcoI+J61048-1~8]

PCR of

[P_cons+RBS+NheI-1, -2, -4, -6, -10, -12]

[P_cons+RBS+SacI-1, -3, -6, -7, -11, -12]

[P_cons+RBS+NcoI+J61048-1~8]

Ligation of

[P_cons+RBS+NheI]Kr

[P_cons+RBS+NcoI]Kr

Electrophoresis of

[XhoI-2, -4, -5](dig)

[SacI-10, -11](dig)

[NheI-20](dig)

[P_cons+RBS+NdeI](dig)

[P_cons+RBS+NcoI](dig)

[PCR NcoI-1~8]

Mini-prep of

[P_cons+RBS+NheI-6]

[P_cons+RBS+SalI-1, -7, -11]

Digestion of

[P_cons+RBS+NheI-6](EP)

[P_cons+RBS+SalI-1, -7, -11](EP)

[P_cons+RBS+NcoI]

[P_cons+RBS+Ndel]

PCR of and cultivation of

[P_cons+RBS+NcoI+J61048]

Mini-prep of

[P_cons+RBS+NcoI+J61048-7]Cr

Electrophoresis of

[P_cons+RBS+KphI+J61048-1]Cr

[P_cons+RBS+SalI+J61048-3]Cr

[P_cons+RBS+AseI+J61048 ]Cr

[P_cons+RBS+NdeI+J61048-1]

[P_cons+RBS+HindIII+J61048-1]

[P_cons+RBS+SacI+J61048 -1]

[P_cons+RBS+NcoI+J61048](dig)

[SacI-11]

DNA Sequencing of

[P_cons+RBS+NdeI]-2 Cr

[P_cons+RBS+NcoI]-2 Cr

[P_cons+RBS+NheI]-6 Cr

[P_cons+RBS+SalI-1]Cr

[P_cons+RBS+SalI-11]Cr

[P_cons+RBS+HindIII-1]Cr

[NcoI-12]

[P_cons+RBS+KpnI-1+J61048]Cr

[P_cons+RBS+AseI+J61048]Cr

[P_cons+RBS+SalI-3+J61048]Cr

[P_cons+RBS+HindIII-1+J61048]Cr

[P_cons+RBS+XhoI-2+J61048]Cr

[P_cons+RBS+XhoI-5+J61048]Cr

[P_cons+RBS+NcoI-5+J61048]Cr

[P_cons+RBS+SacI-11-1+J61048]Cr

[P_cons+RBS+NdeI-1+J61048]Cr

[P_cons+RBS+NheI-20+J61048]Cr

[P_cons+RBS+AseI-7+J61048]Cr

[P_cons+RBS+HindIII-7+J61048]Ar

[P_cons+RBS+SacI-16+J61048]Ar

[P_cons+RBS+SalI-17+J61048]Ar

[P_cons+RBS+NdeI-2+J61048]Cr

[P_cons+RBS+NcoI-2+J61048]Cr

[P_cons+RBS+XhoI+J61048]Ar

[P_cons+RBS+KpnI-7+J61048]Ar

[P_cons+RBS+NheI-11+J61048]Ar

10/7

PCR of

[P_cons+RBS+NheI+J61048]

[P_cons+RBS+SalI in PSB3C5]

[P_cons+RBS+SacI in PSB3C5]

Cultivation of

[P_cons+RBS+NheI+J61048-1, -3, -4, -6, -8, -10, -15, -16]

Transformation of

[P_cons+RBS+XhoI-2 +J61048]

[P_cons+RBS+XhoI-5 +J61048-1]

[P_cons+RBS+SacI-11+J61048]

PCR of

[P_cons+RBS+ NdeI in PSB3C5]

[P_cons+RBS+KpnI in PSB3C5]

[P_cons+RBS+AseI in PSB3C5]

[P_cons+RBS+XhoI in PSB3C5]

[P_cons+RBS+NcoI in PSB3C5]

[P_cons+RBS+NheI in PSB3C5]

nine kinds of PBAN

DNA Sequencing of

[P_cons+RBS+NcoI-5+J61048]

10/8

DNA Sequencing of

[PBAN NcoI]

[P_cons+RBS+SalI -1]

[P_cons+RBS+SalI-11]

[P_cons+RBS+NheI-6]

[P_cons+RBS+xhol-2+RBS+BFP+TER]

[P_cons+RBS+SacI-11+RBS+BFP+TER]

[P_cons+RBS+ Nhel-20+RBS+BFP+TER]

Ligation of

P_cons+RBS+NcoI on psb3c5

10/9

ALL Nhel-9(10/7)x2

ALL SacI-11(10/7)x1

ALL xhol-2(10/7)x1

Electrophoresis of

Gel 1

Well 1 Marker

Well 2 P_cons+RBS+NheI-20+RBS+BFP+TER

Well 3 P_cons+RBS+Hind-1+RBS+BFP+TER

Well 4 P_cons+RBS+KpnI-1+RBS+BFP+TER

Well 5 P_cons+RBS+Ndel-1+RBS+BFP+TER

2 Well 6 P_cons+RBS+NcoI-5+RBS+BFP+TER

Well 7 P_cons+RBS+AseI ALL

Well 8 PBAN NcoI-12

Well 9 P_cons+RBS+NheI-6

Well 10 P_cons+RBS+Hind-1

Well 11 P_cons+RBS+NcoI-20

Well 12 P_cons+RBS+NcoI-2-2

Well 13 P_cons+RBS+Ndel-2-1

Well 14 P_cons+RBS+Nedl-2-2

Well 15 P_cons+RBS+SacI-1

Well 16 P_cons+RBS+SacI-11

Well 17 Marker

Gel 2

Well 1 marker

Well 2 P_cons+RBS+NheI-11

Well 3 P_cons+RBS+Hind3

Well 4 P_cons+RBS+KpnI-7

Well 5 P_cons+RBS+SacI-16

Well 6 P_cons+RBS+SalI-12

Well 7 P_cons+RBS+Xhol

Transformation of

P_cons+RBS+SacI-3 on psb3c5

P_cons+RBS+KpnI-1 on psb3c5

P_cons+RBS+NcoI-1 on psb3c5

Mini-prep of and sequencing of

ALL NheI-2 x1

ALL SacI-11 x1

ALL Xhol-2 X1

10/10

Transformation of

P_cons+RBS+Ndel on psb3c5

P_cons+RBS+NcoI on psb3c5

Electrophoresis of

Well 1 Marker

Well 2 P_cons+RBS+SacI-3 on psb3c5

Well 3 P_cons+RBS+KpnI-1 on psb3c5

Well 4 P_cons+RBS+NcoI-1 on psb3c5

10/12

PCR of and Electrophoresis of

Well 1 P_cons+RBS+NcoI+psb3c5

Well 2 P_cons+RBS+Ndel+psb3c5

None

@@ Line 3: / Line 3: @@
 <div class="calendar">
 <ul class="calendar-week">
-<li>Sunday</li>
+<li>Sun.</li>
-<li>Monday</li>
+<li>Mon.</li>
-<li>Tuesday</li>
+<li>Tue.</li>
-<li>Wednesday</li>
+<li>Wed.</li>
-<li>Thursday</li>
+<li>Thu.</li>
-<li>Friday</li>
+<li>Fri.</li>
-<li>Saturday</li>
+<li>Sat.</li>
 </ul>
 <ul class="calendar-day">
@@ Line 72: / Line 72: @@
 </div>
 <div class="calendar-note">
-<div class="single note">1
+<div class="single note">
+<p>
+<font color="#FF0000"><span style="font-size:1cm;">10/3</span></font><br></p><p>
+<font color="yellow">Cultivation of</font></p><p>
+[P<sub>cons</sub>+RBS+NdeI]Cr</p><p>
+[P<sub>cons</sub>+RBS+NcoI]Cr</p><p>
+[KpnI-1]Cr</p><p>
+ [NdeI-1]Cr</p><p>
+[XhoI-1]Cr</p><p>
+ [SacI-1]Cr</p><p>
+[AseI-1]Cr</p><p>
+[P<sub>cons</sub>+RBS+HindIII-1]Cr</p><p>
+ [P<sub>cons</sub>+RBS+KpnI-4]Cr</p><p>
+ [P<sub>cons</sub>+RBS+AseI-5]Cr</p><p>
+[P<sub>cons</sub>+RBS+XhoI-1]Cr</p><p>
+ [P<sub>cons</sub>+RBS+SacI-1]Cr</p><p>
+[P<sub>cons</sub>+RBS+KpnI-1+J61048]Cr</p><p>
+ [SalI-4]Cr</p><p>
+[P<sub>cons</sub>+RBS+SalI-3+J61048]Cr</p><p>
+[HindIII-4]Cr</p><p>
+[P<sub>cons</sub>+RBS+AseI+J61048]Cr</p><p>
+ [NheI]</p><p>
+ [NcoI]</p><p>
+<font color="yellow">Electrophoresis of</font></p><p>
+ [NdeI](EP)</p><p>
+ [NcoI](EP)</p><p>
+ [SacI](EP)</p><p>
+<font color="yellow">Transformation of</font></p><p>
+ [P<sub>cons</sub>+RBS+NdeI]Cr</p><p>
+ [P<sub>cons</sub>+RBS+SacI]Cr</p><p>
+<font color="yellow">DNA Sequencing of</font></p><p>
+ [P<sub>cons</sub>+RBS+NdeI+J61048-1]</p><p>
+ [P<sub>cons</sub>+RBS+HindIII+J61048-1]</p><p>
+ [P<sub>cons</sub>+RBS+SalI+J61048-4]</p><p>
+ [P<sub>cons</sub>+RBS+SacI+J61048-1]</p><p>
+[P<sub>cons</sub>+RBS+SacI+J61048-1](fail)</p><p>
+<font color="#FF0000"><span style="font-size:1cm;">10/4</span></font><br></p><p>
+<font color="yellow">Digestion of</font></p><p>
+ [P<sub>cons</sub>+RBS+XhoI+J61048-2, -4, -5] </p><p>
+ [P<sub>cons</sub>+RBS+SacI+J61048-10, -11]</p><p>
+[P<sub>cons</sub>+RBS+NheI+J61048-20]</p><p>
+ [P<sub>cons</sub>+RBS+NheI]Cr</p><p>
+ [P<sub>cons</sub>+RBS+NcoI]Cr</p><p>
+<font color="yellow">Cultivation of </font></p><p>
+[P<sub>cons</sub>+RBS+NheI-1, -2, -4, -6, -10, -12]</p><p>
+ [P<sub>cons</sub>+RBS+SalI-1, -3, -6, -7, -11, -12] </p><p>
+<font color="yellow">Cultivation of </font></p><p>
+[P<sub>cons</sub>+RBS+NcoI+J61048-1~8]</p><p>
+<font color="yellow">PCR of</font></p><p>
+ [P<sub>cons</sub>+RBS+NheI-1, -2, -4, -6, -10, -12]</p><p>
+ [P<sub>cons</sub>+RBS+SacI-1, -3, -6, -7, -11, -12]</p><p>
+ [P<sub>cons</sub>+RBS+NcoI+J61048-1~8]</p><p>
+<font color="yellow">Ligation of </font></p><p>
+[P<sub>cons</sub>+RBS+NheI]Kr</p><p>
+ [P<sub>cons</sub>+RBS+NcoI]Kr</p><p>
+<font color="yellow">Electrophoresis of </font></p><p>
+[XhoI-2, -4, -5](dig)</p><p>
+ [SacI-10, -11](dig)</p><p>
+ [NheI-20](dig)</p><p>
+ [P<sub>cons</sub>+RBS+NdeI](dig)</p><p>
+ [P<sub>cons</sub>+RBS+NcoI](dig)</p><p>
+ [PCR NcoI-1~8]</p><p>
+<font color="yellow">Mini-prep of</font></p><p>
+ [P<sub>cons</sub>+RBS+NheI-6]</p><p>
+ [P<sub>cons</sub>+RBS+SalI-1, -7, -11]</p><p>
+<font color="yellow">Digestion of </font></p><p>
+[P<sub>cons</sub>+RBS+NheI-6](EP)</p><p>
+ [P<sub>cons</sub>+RBS+SalI-1, -7, -11](EP)</p><p>
+ [P<sub>cons</sub>+RBS+NcoI]</p><p>
+ [P<sub>cons</sub>+RBS+Ndel]</p><p>
+<font color="yellow">PCR of and cultivation of </font></p><p>
+[P<sub>cons</sub>+RBS+NcoI+J61048]</p><p>
+<font color="yellow">Mini-prep of</font></p><p>
+ [P<sub>cons</sub>+RBS+NcoI+J61048-7]Cr</p><p>
+<font color="yellow">Electrophoresis of </font></p><p>
+[P<sub>cons</sub>+RBS+KphI+J61048-1]Cr</p><p>
+ [P<sub>cons</sub>+RBS+SalI+J61048-3]Cr</p><p>
+ [P<sub>cons</sub>+RBS+AseI+J61048 ]Cr</p><p>
+ [P<sub>cons</sub>+RBS+NdeI+J61048-1]</p><p>
+ [P<sub>cons</sub>+RBS+HindIII+J61048-1]</p><p>
+ [P<sub>cons</sub>+RBS+SacI+J61048 -1]</p><p>
+ [P<sub>cons</sub>+RBS+NcoI+J61048](dig)</p><p>
+ [SacI-11]</p><p>
+<font color="yellow">DNA Sequencing of </font></p><p>
+[P<sub>cons</sub>+RBS+NdeI]-2 Cr</p><p>
+[P<sub>cons</sub>+RBS+NcoI]-2 Cr</p><p>
+[P<sub>cons</sub>+RBS+NheI]-6 Cr</p><p>
+[P<sub>cons</sub>+RBS+SalI-1]Cr</p><p>
+[P<sub>cons</sub>+RBS+SalI-11]Cr</p><p>
+[P<sub>cons</sub>+RBS+HindIII-1]Cr</p><p>
+[NcoI-12]</p><p>
+[P<sub>cons</sub>+RBS+KpnI-1+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+AseI+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+SalI-3+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+HindIII-1+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+XhoI-2+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+XhoI-5+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+NcoI-5+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+SacI-11-1+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+NdeI-1+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+NheI-20+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+AseI-7+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+HindIII-7+J61048]Ar</p><p>
+[P<sub>cons</sub>+RBS+SacI-16+J61048]Ar</p><p>
+[P<sub>cons</sub>+RBS+SalI-17+J61048]Ar</p><p>
+[P<sub>cons</sub>+RBS+NdeI-2+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+NcoI-2+J61048]Cr</p><p>
+[P<sub>cons</sub>+RBS+XhoI+J61048]Ar</p><p>
+[P<sub>cons</sub>+RBS+KpnI-7+J61048]Ar</p><p>
+[P<sub>cons</sub>+RBS+NheI-11+J61048]Ar</p>
 <br></div>
-<div class="single note"><p>2014/10/8
+<div class="single note">
-Sequencing
-. PBAN NcoI
-. Pcons+RBS+SalI -1
-. Pcons+RBS+SalI-11
-. Pcons+RBS+NheI-6
-. Pcons+RBS+xhol-2+RBS+BFP+TER
-. Pcons+RBS+SacI-11+RBS+BFP+TER
-. Pcons+RBS+ Nhel-20+RBS+BFP+TER
-</p>
 <p>
-Ligation
+<font color="#FF0000"><span style="font-size:1cm;">10/7</span></font><br></p><p>
-Member:Mick
+<font color="yellow">PCR of</font></p><p>
-Pcons+RBS+NcoI on psb3c5
+ [P<sub>cons</sub>+RBS+NheI+J61048]</p><p>
-Pcons+RBS+NcoI on psb3c5
+[P<sub>cons</sub>+RBS+SalI in PSB3C5]</p><p>
-</p><p>
+ [P<sub>cons</sub>+RBS+SacI in PSB3C5]</p><p>
-/10/9
+<font color="yellow">Cultivation of</font> </p><p>
-</p><p>
+[P<sub>cons</sub>+RBS+NheI+J61048-1, -3, -4, -6, -8, -10, -15, -16]</p><p>
-Cultivation at 12:00
+<font color="yellow">Transformation of </font></p><p>
-</p>
+[P<sub>cons</sub>+RBS+XhoI-2 +J61048]</p><p>
-ALL Nhel-9(10/7)x2
+ [P<sub>cons</sub>+RBS+XhoI-5 +J61048-1]</p><p>
-ALL SacI-11(10/7)x1
+ [P<sub>cons</sub>+RBS+SacI-11+J61048]</p><p>
-ALL xhol-2(10/7)x1
+<font color="yellow">PCR of</font></p><p>
+  [P<sub>cons</sub>+RBS+ NdeI  in PSB3C5]</p><p>
+ [P<sub>cons</sub>+RBS+KpnI in PSB3C5]</p><p>
+ [P<sub>cons</sub>+RBS+AseI in PSB3C5]</p><p>
+ [P<sub>cons</sub>+RBS+XhoI in PSB3C5]</p><p>
+ [P<sub>cons</sub>+RBS+NcoI in PSB3C5]</p><p>
+ [P<sub>cons</sub>+RBS+NheI in PSB3C5]</p><p>
+ nine kinds of PBAN</p><p>
+<font color="yellow">DNA Sequencing of</font></p><p>
+  [P<sub>cons</sub>+RBS+NcoI-5+J61048]</p><p>
+<font color="#FF0000"><span style="font-size:1cm;">10/8</span></font><br> </p><p>
+<font color="yellow">DNA Sequencing of</font></p><p>
+[PBAN NcoI]</p><p>
+[P<sub>cons</sub>+RBS+SalI -1]</p><p>
+[P<sub>cons</sub>+RBS+SalI-11]</p><p>
+[P<sub>cons</sub>+RBS+NheI-6]</p><p>
+[P<sub>cons</sub>+RBS+xhol-2+RBS+BFP+TER]</p><p>
+[P<sub>cons</sub>+RBS+SacI-11+RBS+BFP+TER]</p><p>
+[P<sub>cons</sub>+RBS+ Nhel-20+RBS+BFP+TER]</p>
 <p>
-Electrophoresis
+<font color="yellow">Ligation of</font></p><p>
-The first piece of gel
+P<sub>cons</sub>+RBS+NcoI on psb3c5</p><p>
-</p>
+P<sub>cons</sub>+RBS+NcoI on psb3c5</p><p>
-.	maker
+<font color="#FF0000"><span style="font-size:1cm;">10/9</span></font><br></p><p>
-.	Pcons+RBS+NheI-20+RBS+BFP+TER
+ALL Nhel-9(10/7)x2</p><p>
-.	Pcons+RBS+Hind-1+RBS+BFP+TER
+ALL SacI-11(10/7)x1</p><p>
-.	Pcons+RBS+KpnI-1+RBS+BFP+TER
+ALL xhol-2(10/7)x1</p><p>
-.	Pcons+RBS+Ndel-1+RBS+BFP+TER
+<font color="yellow">Electrophoresis of</font></p><p>
-.	Pcons+RBS+NcoI-5+RBS+BFP+TER
+Gel 1</p><p>
-.	Pcons+RBS+AseI ALL
+Well 1 Marker</p><p>
-.	PBAN NcoI-12
+Well 2 P<sub>cons</sub>+RBS+NheI-20+RBS+BFP+TER</p><p>
-.	Pcons+RBS+NheI-6
+Well 3 P<sub>cons</sub>+RBS+Hind-1+RBS+BFP+TER</p><p>
-.	Pcons+RBS+Hind-1
+Well 4 P<sub>cons</sub>+RBS+KpnI-1+RBS+BFP+TER </p><p>
-.	Pcons+RBS+NcoI-20
+Well 5 P<sub>cons</sub>+RBS+Ndel-1+RBS+BFP+TER</p><p>2
-.	Pcons+RBS+NcoI-2-2
+Well 6 P<sub>cons</sub>+RBS+NcoI-5+RBS+BFP+TER</p><p>
-.	Pcons+RBS+Ndel-2-1
+Well 7 P<sub>cons</sub>+RBS+AseI ALL</p><p>
-.	Pcons+RBS+Nedl-2-2
+Well 8 PBAN NcoI-12</p><p>
-.	Pcons+RBS+SacI-1
+Well 9 P<sub>cons</sub>+RBS+NheI-6</p><p>
-.	Pcons+RBS+SacI-11
+Well 10 P<sub>cons</sub>+RBS+Hind-1</p><p>
-.	Maker
+Well 11 P<sub>cons</sub>+RBS+NcoI-20</p><p>
-<p>
+Well 12 P<sub>cons</sub>+RBS+NcoI-2-2</p><p>
-The second piece of gel
+Well 13 P<sub>cons</sub>+RBS+Ndel-2-1</p><p>
-Member: Chris Ho
+Well 14 P<sub>cons</sub>+RBS+Nedl-2-2</p><p>
-</p><p>
+Well 15 P<sub>cons</sub>+RBS+SacI-1</p><p>
-.	maker
+Well 16 P<sub>cons</sub>+RBS+SacI-11</p><p>
-.	Pcons+RBS+NheI-11
+Well 17 Marker</p><p>
-.	Pcons+RBS+Hind3
+Gel 2</p><p>
-.	Pcons+RBS+KpnI-7
+Well 1 marker</p><p>
-.	Pcons+RBS+SacI-16
+Well 2 P<sub>cons</sub>+RBS+NheI-11</p><p>
-.	Pcons+RBS+SalI-12
+Well 3 P<sub>cons</sub>+RBS+Hind3</p><p>
-.	Pcons+RBS+Xhol
+Well 4 P<sub>cons</sub>+RBS+KpnI-7</p><p>
+Well 5 P<sub>cons</sub>+RBS+SacI-16</p><p>
+Well 6 P<sub>cons</sub>+RBS+SalI-12</p><p>
+Well 7 P<sub>cons</sub>+RBS+Xhol</p><p>
+<font color="yellow">Transformation of</font></p><p>
+P<sub>cons</sub>+RBS+SacI-3 on psb3c5</p><p>
+P<sub>cons</sub>+RBS+KpnI-1 on psb3c5</p><p>
+P<sub>cons</sub>+RBS+NcoI-1 on psb3c5
 </p><p>
+<font color="yellow">Mini-prep of and sequencing of</font></p><p>
+ALL NheI-2 x1</p><p>
+ALL SacI-11 x1</p><p>
+ALL Xhol-2 X1</p><p>
+<font color="#FF0000"><span style="font-size:1cm;">10/10</span></font><br></p><p>
+<font color="yellow">Transformation of </font></p><p>
+P<sub>cons</sub>+RBS+Ndel on psb3c5</p><p>
+P<sub>cons</sub>+RBS+NcoI on psb3c5</p><p>
+<font color="yellow">Electrophoresis of </font></p><p>
-Start at 2:15
+Well 1 Marker</p><p>
-.Pcons+RBS+SacI-3 to psb3c5
+Well 2 P<sub>cons</sub>+RBS+SacI-3 on psb3c5</p><p>
-. Pcons+RBS+KpnI-1 to psb3c5
+Well 3 P<sub>cons</sub>+RBS+KpnI-1 on psb3c5</p><p>
-. Pcons+RBS+NcoI-1 to psb3c5
+Well 4 P<sub>cons</sub>+RBS+NcoI-1 on psb3c5</p><p>
-</p><p>
-Mini-prep and sequencing
-.	ALL NheI-2 x1
-.	ALL SacI-11 x1
-.	ALL Xhol-2 X1
-</p><p>
-/10/10
-Transform
-Start at 8:45
-Pcons+RBS+Ndel on psb3c5
-Pcons+RBS+NcoI on psb3c5
-</p><p>
-Electrophoresis
-.	maker
-.	Pcons+RBS+SacI-3 on psb3c5
-.	Pcons+RBS+KpnI-1 on psb3c5
-.	Pcons+RBS+NcoI-1 on psb3c5
-</p>
+<br></div>
+<div class="single note">
+<p>
+<font color="#FF0000"><span style="font-size:1cm;">10/12</span></font><br></p><p>
+<font color="yellow">PCR of and Electrophoresis of</font></p><p>
+Well 1 P<sub>cons</sub>+RBS+NcoI+psb3c5</p><p>
+Well 2 P<sub>cons</sub>+RBS+Ndel+psb3c5</p>
 <br></div>
-<div class="single note"><p>
+<div class="single note">
-/10/10
+<p>
-Transform
+<font color="#FF0000"><span style="font-size:1cm;">None</span></font><br></p><p>
-Start at 8:45
-Pcons+RBS+Ndel on psb3c5
-Pcons+RBS+NcoI on psb3c5
-Electrophoresis
-.	maker
-.	Pcons+RBS+SacI-3 on psb3c5
-.	Pcons+RBS+KpnI-1 on psb3c5
-.	Pcons+RBS+NcoI-1 on psb3c5
-</p><p>
-/10/12
-PCR and Electrophoresis
-.	Pcons+RBS+NcoI+psb3c5
-.	Pcons+RBS+Ndel+psb3c5
 </p>
 <br></div>
-<div class="single note">4
+<div class="single note">
-<br></div>
+<p>
-<div class="single note">5
+<font color="#FF0000"><span style="font-size:1cm;">None</span></font><br></p><p>
+</p>
 <br></div>
 <div class"clear"></div>
 </div>