Team:NCTU Formosa/Notebook/test2

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Revision as of 13:18, 2 October 2014

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5/1 Cultivation of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2) Transform of [T7 polymerase]Cr (G2)

5/2 Cultivation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1) Mini-prep of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2) Digestion of [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and [B0015]Ar*2(ES & XP) (G2)

5/3 Mini-prep of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1) Mini-pep of [B0032] Ar, [B0015] Ar,[BFP] Cr (G3) Digestion of [J23101] Ar (ES),[K592100]Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3] Cr(EP),[PSB1K3] Kr(EP) mini (G1) Electrophoresis of plasmid and digested product of [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and B0015*2(ES & XP) (G2) Electrophoresis of digested product of [J23101] Ar (ES),[K592100] Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3]Cr(EP),[PSB1K3] Kr(EP) (G1) Cultivation of [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1) Ligation: [BFP+B0032+PSB1K3]Kr (G2) Ligation of [J23101+B0032+PSB1C3] Cr (G1) Transform of ligation product [BFP+B0032+PSB1K3]Kr (G2) Transform of [T7polymerase]Cr and [B0015]Ar (G2)

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 <li>23</li>
 <li>24</li>
 </ul>
 </div>
 <div class="calendar-note">
-<div  class="single note">none<br></div>
+<div class="single note">
-<div  class="single note">
-<p>4/11
-Transform of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr  (G2)
-Transform of [Plac+PSB1A3] Ar, [mGFP+PSB1A3] Ar  (G3)
-Transform of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr  (G1)
 <p>
-<p>4/12
+/1
-Cultivation of  [PSB1K3] Kr, [PSB1C3] Cr (G2)
+Cultivation of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)
-Cultivation of  [PSB1A3] Ar, [PSB1C3] Cr (G1)
+Transform of [T7 polymerase]Cr (G2)</p>
-Cultivation of [mGFP] Ar (G3)
-</p>
-<br></div>
-<div class="single note">
-<p>4/13
+<p>5/2
-Mini-prep of [mGFP] Ar, [PSB1K3] Kr(G3)
+Cultivation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
-Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2)
+Mini-prep of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)
-Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)</p>
+Digestion of [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and [B0015]Ar*2(ES & XP) (G2)</p>
-<p>4/17
+<p>5/3
-Transform of [PSB1K3] Kr (G1)</p>
+Mini-prep of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
+Mini-pep of [B0032] Ar, [B0015] Ar,[BFP] Cr (G3)
-<p>4/18
+Digestion of [J23101] Ar (ES),[K592100]Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3] Cr(EP),[PSB1K3] Kr(EP) mini (G1)
-Cultivation of [PSB1K3] Kr (G1) </p>
+Electrophoresis of plasmid and digested product of [PSB1C3]Cr(EP), [BFP]Cr(XP),  [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and  B0015*2(ES & XP) (G2)
+Electrophoresis of digested product of [J23101] Ar (ES),[K592100] Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3]Cr(EP),[PSB1K3] Kr(EP) (G1)
-<p>4/19
+Cultivation of [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1)
-Transform of [Plac+PSB1A3] Ar, [PSB1K3] Kr , [PSB1A3] Ar, [PSB1C3] Cr (G3)
+Ligation: [BFP+B0032+PSB1K3]Kr (G2)
-Mini-prep of [PSB1K3] Kr(G1) </p>
+Ligation of [J23101+B0032+PSB1C3] Cr (G1)
+Transform of ligation product [BFP+B0032+PSB1K3]Kr (G2)
+Transform of [T7polymerase]Cr and [B0015]Ar (G2)</p><br></div>
+<div  class="single note">
 <br></div>
 <div class="single note">
-<p>4/20
-Transform of [PSB1A3] Ar (G2)
-Electrophoresis of [PSB1K3]Kr, [PSB1C3]Cr plasmid (G2)</p>
-<p>4/21
-Cultivation of  [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)</p>
-<p>4/22
-Mini-prep of  [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)</p>
 <br></div>
 <div class="single note">
-ssss
-<br></div>
+<br></div>
 </div>
 <div class="clear"></div>
 </div>