Team:NCTU Formosa/Notebook/test2

From 2014.igem.org

(Difference between revisions)
Line 49: Line 49:
</div>
</div>
<div class="calendar-note">
<div class="calendar-note">
-
<div class="single note"><p>
+
<div class="single note">
 +
<p>
5/1
5/1
Cultivation of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)
Cultivation of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)

Revision as of 13:05, 2 October 2014

  • Sunday
  • Monday
  • Tuesday
  • Wednesday
  • Thursday
  • Friday
  • Saturday
  •  
  •  
  •  
  •  
  • 1
  • 2
  • 3
  • 4
  • 5
  • 6
  • 7
  • 8
  • 9
  • 10
  • 11
  • 12
  • 13
  • 14
  • 15
  • 16
  • 17
  • 18
  • 19
  • 20
  • 21
  • 22
  • 23
  • 24

5/1 Cultivation of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2) Transform of [T7 polymerase]Cr (G2)

5/2 Cultivation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1) Mini-prep of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2) Digestion of [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and [B0015]Ar*2(ES & XP) (G2)

5/3 Mini-prep of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1) Mini-pep of [B0032] Ar, [B0015] Ar,[BFP] Cr (G3) Digestion of [J23101] Ar (ES),[K592100]Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3] Cr(EP),[PSB1K3] Kr(EP) mini (G1) Electrophoresis of plasmid and digested product of [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and B0015*2(ES & XP) (G2) Electrophoresis of digested product of [J23101] Ar (ES),[K592100] Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3]Cr(EP),[PSB1K3] Kr(EP) (G1) Cultivation of [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1) Ligation: [BFP+B0032+PSB1K3]Kr (G2) Ligation of [J23101+B0032+PSB1C3] Cr (G1) Transform of ligation product [BFP+B0032+PSB1K3]Kr (G2) Transform of [T7polymerase]Cr and [B0015]Ar (G2)