Team:Michigan

From 2014.igem.org

(Difference between revisions)
 
(84 intermediate revisions not shown)
Line 1: Line 1:
-
{{CSS/Main}}
+
<! -- {{MichiganHeadCSS/Meta}} -->
-
 
+
-
 
+
<html>
<html>
 +
<body>
 +
<div id = "main-wrap">
 +
 +
<div id = "announcement" class = "container-wrap">
 +
<div id = "announcement-container" class = "container">
 +
<div id = "announcement-content">
 +
<p>
 +
This is an announcement, click the Close button on the right and it will not show again until you restart your browser.
 +
</p>
 +
</div><!--- #announcement-content -->
 +
 +
<a href = "#" class = "close-announcement">Close</a>
 +
</div><!--- #announcement-container -->
 +
</div><!--- #announcement -->
 +
 +
 +
 +
<div id = "cap" class = "container-wrap"></div><!--- #cap -->
 +
 +
 +
 +
<header id = "header" class = "container-wrap">
 +
<div id = "header-container" class = "container">
 +
 +
<div id = "logo" style="width:150px;height:150px">
-
<!--main content -->
+
<a id = "site-title" href = "index.html">
-
<table width="70%" align="center">
+
<img src = "https://static.igem.org/mediawiki/2014/6/61/Umichsigil.jpg" alt = "MSBT" style="width:110px;height:110px;position:absolute;top:-76px;left:1px" />
-
 
+
</a><!--- #site-title -->
-
 
+
-
<!--welcome box -->
+
<span id = "site-description">
-
<tr>
+
</span><!--- #site-description -->
-
<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
+
</div><!--- #logo -->
-
<h1 >WELCOME TO iGEM 2014! </h1>
+
-
<p>Your team has been approved and you are ready to start the iGEM season!
+
<nav id = "main-nav-menu">
-
<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
+
<ul>
-
<br>
+
<li class = "nav-menu-item current">
-
<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Michigan&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
+
<a href = "https://2014.igem.org/Team:Michigan">Home</a>
-
</td>
+
</li>
-
</tr>
+
-
 
+
<li class = "nav-menu-item">
-
<tr> <td colspan="3"  height="5px"> </td></tr>
+
<a href = "https://2014.igem.org/Team:Michigan/Project/">Project</a>
-
<!-- end welcome box -->
+
<ul class = "sub-menu">
-
<tr>  
+
<li class = "nav-menu-item">
-
 
+
<a href = "https://2014.igem.org/Team:Michigan/Project/">Background</a>
-
<!--navigation menu -->
+
</li>
-
<td align="center" colspan="3">
+
-
 
+
<li class = "nav-menu-item">
-
<table  width="100%">
+
<a href = "https://2014.igem.org/Team:Michigan/Design/">Design</a>
-
<tr heigth="15px"></tr>
+
</li>
-
<tr heigth="75px">  
+
-
 
+
<li class = "nav-menu-item">
-
 
+
<a href = "https://2014.igem.org/Team:Michigan/Modeling/">Modeling</a>
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
</li>
-
<a href="https://2014.igem.org/Team:Michigan"style="color:#000000">Home </a> </td>
+
-
 
+
<li class = "nav-menu-item">
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
<a href = "https://2014.igem.org/Team:Michigan/Results/">Results</a>
-
<a href="https://2014.igem.org/Team:Michigan/Team"style="color:#000000"> Team </a> </td>
+
</li>
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
-
<a href="https://igem.org/Team.cgi?year=2014&team_name=Michigan"style="color:#000000"> Official Team Profile </a></td>
+
<li class = "nav-menu-item">
-
 
+
<a href = "https://2014.igem.org/Team:Michigan/Future/">Future Plans</a>
-
<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
</li>
-
<a href="https://2014.igem.org/Team:Michigan/Project"style="color:#000000"> Project</a></td>
+
-
 
+
<li class = "nav-menu-item">
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
<a href = "https://2014.igem.org/Team:Michigan/Accomplishments/">Accomplishments</a>
-
<a href="https://2014.igem.org/Team:Michigan/Parts"style="color:#000000"> Parts</a></td>
+
</li>
-
 
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
</ul>
-
<a href="https://2014.igem.org/Team:Michigan/Modeling"style="color:#000000"> Modeling</a></td>
+
</li>
-
 
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
<li class = "nav-menu-item">
-
<a href="https://2014.igem.org/Team:Michigan/Notebook"style="color:#000000"> Notebook</a></td>
+
<a href = "https://2014.igem.org/Team:Michigan/Human/">Human Practice</a>
-
 
+
<ul class = "sub-menu">
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
<li class = "nav-menu-item">
-
<a href="https://2014.igem.org/Team:Michigan/Safety"style=" color:#000000"> Safety </a></td>
+
<a href = "https://2014.igem.org/Team:Michigan/Outreach/">Outreach</a>
-
 
+
</li>
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
-
<a href="https://2014.igem.org/Team:Michigan/Attributions"style="color:#000000"> Attributions </a></td>
+
<li class = "nav-menu-item">
-
 
+
<a href = "https://2014.igem.org/Team:Michigan/Economic/">Economic Analysis</a>
-
 
+
-
<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
+
</li>
-
</tr>
+
-
</table>
+
<li class = "nav-menu-item">
-
 
+
<a href = "https://2014.igem.org/Team:Michigan/Covance/">Covance Partnership</a>
-
<!--end navigation menu -->
+
</li>
-
</tr>
+
-
 
+
<li class = "nav-menu-item">
-
 
+
<a href = "https://2014.igem.org/Team:Michigan/Collaborations/">Collaborations</a>
-
</tr>
+
</li>
-
+
-
 
+
-
 
+
-
 
+
-
 
+
-
</td>
+
-
 
+
-
<tr> <td colspan="3"  height="15px"> </td></tr>
+
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
+
-
<tr> <td colspan="3"  height="5px"> </td></tr>
+
-
 
+
-
 
+
-
<!--requirements section -->
+
-
<tr><td colspan="3"> <h3> Requirements </h3></td></tr>
+
-
<tr>
+
-
<td width="45%"  valign="top">
+
-
 
+
-
<p> Please be sure to keep these links, your audience will want to find your: </p>
+
-
 
+
-
<!-- Links to other team pages -->
+
-
<ul>
+
-
<li><a href="https://2014.igem.org/Team:Michigan">Home</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Michigan/Team">Team</a> </li>
+
-
<li><a href="https://igem.org/Team.cgi?year=2013&team_name=Michigan">Official Team Profile</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Michigan/Project">Project</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Michigan/Parts">Parts</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Michigan/Modeling">Modeling</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Michigan/Notebook">Notebook</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Michigan/Safety">Safety</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:Michigan/Attributions">Attributions</a> </li>
+
-
 
+
-
</ul>
+
-
 
+
-
</td>
+
-
 
+
-
<td > </td>
+
-
<td width="45%">
+
-
 
+
-
<p>There are a few wiki requirements teams must follow:</p>
+
-
<ul>
+
-
<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
+
-
<li>All pages must be created under the team’s name space.</li>
+
-
<li>As part of your documentation, keep the links from the menu to the left. </li>
+
-
<li>Do not use flash in wiki code. </li>
+
-
<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
+
-
</ul>
+
-
<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
+
-
 
+
-
</td>
+
-
</tr>
+
-
 
+
-
 
+
-
<tr> <td colspan="3"  height="15px"> </td></tr>
+
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1"> </tr>
+
-
 
+
-
 
+
-
<!--tips  -->
+
-
<tr><td colspan="3" > <h3> Tips  </h3></td></tr>
+
-
 
+
-
<tr>
+
-
<td width="45%" valign="top">
+
-
<p>We are currently working on providing teams with some easy to use design templates.
+
-
<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
+
-
 
+
-
<ul>
+
-
<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
+
-
<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
+
-
<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
+
-
<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
+
-
<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
+
-
</ul>
+
-
<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
+
-
</td>
+
</ul>
 +
</li>
 +
 +
<li class = "nav-menu-item">
 +
<a href = "https://2014.igem.org/Team:Michigan/Notebook/">Notebook</a>
 +
</li>
-
<td> </td>
+
<li class = "nav-menu-item">
-
<td width="45%">  
+
<a href = "https://2014.igem.org/Team:Michigan/Team/">Team</a>
 +
<ul class = "sub-menu">
 +
<li class = "nav-menu-item">
 +
<a href = "https://2014.igem.org/Team:Michigan/Team/"> Team Members </a>
 +
</li>
 +
<li class = "nav-menu-item">
 +
<a href = "https://2014.igem.org/Team:Michigan/Sponsors"> Sponsors </a>
 +
</li>
 +
</li>
-
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
</ul>
 +
</nav><!--- #main-nav-menu -->
 +
 +
<select id = "responsive-main-nav-menu" onchange = "javascript:window.location.replace(this.value);"></select>
 +
 +
</div><!--- #header-container -->
 +
</header><!--- #header -->
 +
<!--- <div style="background-color:#f1ed6f;width:994px;height:120px;position:absolute;left:-5px;top:51px"></div> -->
 +
 +
 +
<section id = "page" class = "container-wrap">
 +
<div id = "page-container" class = "container">
 +
 +
<header id = "page-header" class = "center">,
 +
<h1><font size="10"> MSBT</font></h1></p><font size="3"><font color="#191970"> University of Michigan iGEM Team </font></font color></p>
-
<ul>
+
</header><!--- #page-header -->
-
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
-
<li>Be clear about what you are doing and what you plan to do.</li>
+
<div style="height:400px;width:450px;position:relative;top:-7px"> <img src="https://static.igem.org/mediawiki/2014/4/46/2website_front.png" style="width:450px;height:400px;position:relative;left:213px"> </div>
-
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
<div style="width:1000px"></div>
-
<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
+
</div>
-
<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
+
<p style="position:relative;width:903px;top:-14px;left:39px"> <font color="#888888"> Antibodies allow us to bind targets in a highly selective manner, giving them a paramount role in biotechnology and medicine. Antibody production remains difficult in <I>E. coli</I> because antibodies require an oxidizing environment to properly fold. We have constructed an expression system capable of secreting functional scFv (single chain fragment variable) antibodies by tagging them to OsmY, a naturally secreted protein. Fusions with OsmY are carried through the periplasm into the media; allowing disulfide bonds to form during translocation. We've demonstrated the utility of this system in the context of sensing <i>Salmonella</i>, the pathogen responsible for 23,000 hospitalizations in the US each year. To demonstrate efficacy of our purified scFv antibodies to bind antigen, we compared them to scFv antibodies purified from an industrial system using ELISA. Our construct has the potential of becoming a cost-effective industry applicable method for purifying scFv antibodies, as well as other proteins, in large quantities. </font>
-
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
+
</p>
-
<li>Have lots of fun! </li>
+
<p style="position:absolute;top:566px;left:38px"><font size="6"> <u>Abstract</u></font></p>
-
</ul>
+
</div><!--- #page-content -->
-
</br>
+
-
</td>
+
</div><!--- #page-container -->
-
</tr>
+
</section><!--- #page -->
-
</table>
+
</div><!--- #main-wrap -->
 +
</body>
 +
        <style>
 +
        pre,  p:first-child{
 +
            display: none;
 +
        }
 +
        </style>
 +
</html>
 +
{{MichiganMainScript}}
 +
{{MichiganIEScripts}}

Latest revision as of 03:27, 18 October 2014

<! -- Michigan Synthetic Biology Team -->

This is an announcement, click the Close button on the right and it will not show again until you restart your browser.

Close
,

MSBT

University of Michigan iGEM Team

Antibodies allow us to bind targets in a highly selective manner, giving them a paramount role in biotechnology and medicine. Antibody production remains difficult in E. coli because antibodies require an oxidizing environment to properly fold. We have constructed an expression system capable of secreting functional scFv (single chain fragment variable) antibodies by tagging them to OsmY, a naturally secreted protein. Fusions with OsmY are carried through the periplasm into the media; allowing disulfide bonds to form during translocation. We've demonstrated the utility of this system in the context of sensing Salmonella, the pathogen responsible for 23,000 hospitalizations in the US each year. To demonstrate efficacy of our purified scFv antibodies to bind antigen, we compared them to scFv antibodies purified from an industrial system using ELISA. Our construct has the potential of becoming a cost-effective industry applicable method for purifying scFv antibodies, as well as other proteins, in large quantities.

Abstract

Retrieved from "http://2014.igem.org/Team:Michigan"