Team:Michigan

From 2014.igem.org

(Difference between revisions)
 
(19 intermediate revisions not shown)
Line 25: Line 25:
<div id = "header-container" class = "container">
<div id = "header-container" class = "container">
-
<div id = "logo">
+
<div id = "logo" style="width:150px;height:150px">
 +
 
<a id = "site-title" href = "index.html">
<a id = "site-title" href = "index.html">
-
<img src = "http://che.engin.umich.edu/enewsletter/winter2013/images/MSBTlogo2.jpg" alt = "MSBT" />
+
<img src = "https://static.igem.org/mediawiki/2014/6/61/Umichsigil.jpg" alt = "MSBT" style="width:110px;height:110px;position:absolute;top:-76px;left:1px" />
</a><!--- #site-title -->
</a><!--- #site-title -->
<span id = "site-description">
<span id = "site-description">
-
2014 Michigan Synthetic Biology Team
 
</span><!--- #site-description -->
</span><!--- #site-description -->
</div><!--- #logo -->
</div><!--- #logo -->
Line 45: Line 45:
<ul class = "sub-menu">
<ul class = "sub-menu">
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "elements.html">Background</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Project/">Background</a>
</li>
</li>
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "columns.html">Design</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Design/">Design</a>
</li>
</li>
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "columns.html">Wet Lab</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Modeling/">Modeling</a>
</li>
</li>
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "columns.html">Dry Lab</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Results/">Results</a>
</li>
</li>
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "columns.html">Future Plans</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Future/">Future Plans</a>
</li>
</li>
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "columns.html">Accomplishments</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Accomplishments/">Accomplishments</a>
</li>
</li>
Line 73: Line 73:
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "portfolio_filterable.html">Human Practice</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Human/">Human Practice</a>
<ul class = "sub-menu">
<ul class = "sub-menu">
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "#">Outreach</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Outreach/">Outreach</a>
</li>
</li>
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "#">Econmic Analysis</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Economic/">Economic Analysis</a>
</li>
</li>
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "portfolio_filterable.html">Covance Partnership</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Covance/">Covance Partnership</a>
</li>
</li>
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "portfolio_filterable.html">Collaborations</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Collaborations/">Collaborations</a>
</li>
</li>
Line 97: Line 97:
<li class = "nav-menu-item">
<li class = "nav-menu-item">
-
<a href = "blog.html">Notebook</a>
+
<a href = "https://2014.igem.org/Team:Michigan/Notebook/">Notebook</a>
-
<ul class = "sub-menu">
+
-
<li class = "nav-menu-item">
+
-
<a href = "blog.html">Winter Planning</a>
+
-
</li>
+
-
+
-
<li class = "nav-menu-item">
+
-
<a href = "blog_single.html">Project Journal</a>
+
-
</li>
+
-
</ul>
+
</li>
</li>
Line 127: Line 118:
</div><!--- #header-container -->
</div><!--- #header-container -->
</header><!--- #header -->
</header><!--- #header -->
-
+
<!--- <div style="background-color:#f1ed6f;width:994px;height:120px;position:absolute;left:-5px;top:51px"></div> -->
Line 134: Line 125:
<header id = "page-header" class = "center">,
<header id = "page-header" class = "center">,
-
<h1><font size="10" <em class = "colored">MSBT</em></font></h1></p><font size="3"><font color="#191970"> University of Michigan iGEM Team </font></font color></p>
+
<h1><font size="10"> MSBT</font></h1></p><font size="3"><font color="#191970"> University of Michigan iGEM Team </font></font color></p>
 +
 
</header><!--- #page-header -->
</header><!--- #page-header -->
-
+
-
<div id = "page-content">
+
<div style="height:400px;width:450px;position:relative;top:-7px"> <img src="https://static.igem.org/mediawiki/2014/4/46/2website_front.png" style="width:450px;height:400px;position:relative;left:213px"> </div>
-
+
<div style="width:1000px"></div>
-
<div class = "has-divider">
+
-
<div class = "flexslider">
+
-
<ul class = "slides">
+
-
<li><img alt = "Image" src = "assets/home_slides/responsive.jpg" /></li>
+
-
<li><img alt = "Image" src = "assets/home_slides/html5.jpg" /></li>
+
-
<li><img alt = "Image" src = "assets/home_slides/awesome.jpg" /></li>
+
-
</ul>
+
-
</div>
+
</div>
</div>
-
+
<p style="position:relative;width:903px;top:-14px;left:39px"> <font color="#888888"> Antibodies allow us to bind targets in a highly selective manner, giving them a paramount role in biotechnology and medicine. Antibody production remains difficult in <I>E. coli</I> because antibodies require an oxidizing environment to properly fold. We have constructed an expression system capable of secreting functional scFv (single chain fragment variable) antibodies by tagging them to OsmY, a naturally secreted protein. Fusions with OsmY are carried through the periplasm into the media; allowing disulfide bonds to form during translocation. We've demonstrated the utility of this system in the context of sensing <i>Salmonella</i>, the pathogen responsible for 23,000 hospitalizations in the US each year. To demonstrate efficacy of our purified scFv antibodies to bind antigen, we compared them to scFv antibodies purified from an industrial system using ELISA. Our construct has the potential of becoming a cost-effective industry applicable method for purifying scFv antibodies, as well as other proteins, in large quantities. </font>
-
<div class = "portfolio portfolio-4 row has-divider">
+
</p>
-
<div class = "span-3">
+
<p style="position:absolute;top:566px;left:38px"><font size="6"> <u>Abstract</u></font></p>
-
<h3 class = "fancy-title">General Overview
+
-
+
-
<p>
+
-
What we did, in laymans terms and the general accomplishments
+
-
</p>
+
-
</div>
+
-
+
-
<div class = "span-3">
+
-
<article class = "entry">
+
-
<div class = "entry-wrap">
+
-
<div class = "entry-feature">
+
-
<a href = "https://2014.igem.org/Team:Michigan/Project/" class = "overlay colorbox" title = "Project">
+
-
<span class = "overlay-color"></span>
+
-
<span class = "overlay-icon zoom"></span>
+
-
<img alt = "Image" src = "https://static.igem.org/mediawiki/2014/a/a6/Michigan_Construct_Protein.png" height="225"/>
+
-
</a>
+
-
</div><!-- .entry-feature -->
+
-
+
-
<div class = "entry-body">
+
-
<header>
+
-
<h2 class = "entry-title">
+
-
<a href = "#">About Our Project</a>
+
-
</h2><!-- .entry-title -->
+
-
</header>
+
-
</div><!-- .entry-body -->
+
-
</div><!-- .entry-wrap -->
+
-
</article>
+
-
</div>
+
-
+
-
<div class = "span-3">
+
-
<article class = "entry">
+
-
<div class = "entry-wrap">
+
-
<div class = "entry-feature">
+
-
<a href = "https://2014.igem.org/Team:Michigan/Human_Practice/" class = "overlay colorbox" title = "Human Practice">
+
-
<span class = "overlay-color"></span>
+
-
<span class = "overlay-icon zoom"></span>
+
-
<img alt = "Image" src = "https://2014.igem.org/Team:Michigan/Human_Practice/" />
+
-
</a>
+
-
</div><!-- .entry-feature -->
+
-
+
-
<div class = "entry-body">
+
-
<header>
+
-
<h2 class = "entry-title">
+
-
<a href = "#">Human Practice and Outreach</a>
+
-
</h2><!-- .entry-title -->
+
-
</header>
+
-
</div><!-- .entry-body -->
+
-
</div><!-- .entry-wrap -->
+
-
</article>
+
-
</div>
+
-
+
-
<div class = "span-3">
+
-
<article class = "entry">
+
-
<div class = "entry-wrap">
+
-
<div class = "entry-feature">
+
-
<a href = "https://2014.igem.org/Team:Michigan/Team/" class = "overlay colorbox" title = "Team">
+
-
<span class = "overlay-color"></span>
+
-
<span class = "overlay-icon zoom"></span>
+
-
<img alt = "Image" src = "https://static.igem.org/mediawiki/2014/4/4c/MSBTNewLogo.png" />
+
-
</a>
+
-
</div><!-- .entry-feature -->
+
-
+
-
<div class = "entry-body">
+
-
<header>
+
-
<h2 class = "entry-title">
+
-
<a href = "#">The Team, The Team, The Team</a>
+
-
</h2><!-- .entry-title -->
+
-
</header>
+
-
</div><!-- .entry-body -->
+
-
</div><!-- .entry-wrap -->
+
-
</article>
+
-
</div>
+
-
</div>
+
-
+
-
<div class = "row has-divider clients-grid">
+
-
<div class = "span-3">
+
-
<h3 class = "fancy-title">Project Implications</h3>
+
-
+
-
<p>
+
-
Explain here what the implications of our project is in terms of antibodies, brief overview
+
-
</p>
+
-
</div>
+
-
+
-
</div>
+
-
 
+
</div><!--- #page-content -->
</div><!--- #page-content -->

Latest revision as of 03:27, 18 October 2014

<! -- Michigan Synthetic Biology Team -->

This is an announcement, click the Close button on the right and it will not show again until you restart your browser.

Close
,

MSBT

University of Michigan iGEM Team

Antibodies allow us to bind targets in a highly selective manner, giving them a paramount role in biotechnology and medicine. Antibody production remains difficult in E. coli because antibodies require an oxidizing environment to properly fold. We have constructed an expression system capable of secreting functional scFv (single chain fragment variable) antibodies by tagging them to OsmY, a naturally secreted protein. Fusions with OsmY are carried through the periplasm into the media; allowing disulfide bonds to form during translocation. We've demonstrated the utility of this system in the context of sensing Salmonella, the pathogen responsible for 23,000 hospitalizations in the US each year. To demonstrate efficacy of our purified scFv antibodies to bind antigen, we compared them to scFv antibodies purified from an industrial system using ELISA. Our construct has the potential of becoming a cost-effective industry applicable method for purifying scFv antibodies, as well as other proteins, in large quantities.

Abstract

Retrieved from "http://2014.igem.org/Team:Michigan"