http://2014.igem.org/wiki/index.php?title=Team:London_BioHackspace/Parts&feed=atom&action=historyTeam:London BioHackspace/Parts - Revision history2024-03-29T05:01:10ZRevision history for this page on the wikiMediaWiki 1.16.5http://2014.igem.org/wiki/index.php?title=Team:London_BioHackspace/Parts&diff=401758&oldid=prevCzechton at 03:59, 18 October 20142014-10-18T03:59:57Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Aims</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Aims</h2></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul><li><p>Produce a biobrick containing <em>ompR</em> and sequence it to provide a reliable part for future use in expression of the the Cph8 light sensitive signal transduction pathway in non <em>E. coli</em> chassis.</p></li><li><p>Provide clarifying notes in the registry on parts which have confusingly used the term "ompR" or "OmpR" to label the ompC promoter (ompCp) sequence. Or in some cases have labelled the sequence of ompR as "envZ".</p></li><li><p>Produce a composite biobrick for use in a broad host-range vector such as <a href="https://2014.igem.org/Team:Imperial/Gluconacetobacter">pSEVA321, pSEVA331, pSEVA351</a> which will show whether a a given non-<em>E. coli</em> chassis can functionally express the Cph8 light sensitive signal transduction pathway.</p></li><li><p>Produce a composite biobrick to control the production of extracellular cellulose in <em>dgc1</em> knockout mutants of <em>G. hansenii</em>.</p></li></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul><li><p>Produce a biobrick containing <em>ompR</em> and sequence it to provide a reliable part for future use in expression of the the Cph8 light sensitive signal transduction pathway in non <em>E. coli</em> chassis.</p></li><li><p>Provide clarifying notes in the registry on parts which have confusingly used the term "ompR" or "OmpR" to label the ompC promoter (ompCp) sequence. Or in some cases have labelled the sequence of ompR as "envZ".</p></li><li><p>Produce a composite biobrick for use in a broad host-range vector such as <a href="https://2014.igem.org/Team:Imperial/Gluconacetobacter">pSEVA321, pSEVA331, pSEVA351</a> which will show whether a a given non-<em>E. coli</em> chassis can functionally express the Cph8 light sensitive signal transduction pathway.</p></li><li><p>Produce a composite biobrick to control the production of extracellular cellulose in <em>dgc1</em> knockout mutants of <em>G. hansenii</em>.</p></li></ul></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><strong><a href="http://parts.igem.org/Part:BBa_K1337003">BBa_K1337003</a></strong></br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><strong><a href="http://parts.igem.org/Part:BBa_K1337003">BBa_K1337003</a></strong></br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Light mediated <em>dgc1</em> transcription construct (should produce the cellular pathway in Figure 1.)</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Light mediated <em>dgc1</em> transcription construct (should produce the cellular pathway in Figure 1.)</p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><div style="float: right; background-color: #EFEFEF;"><img style="width: 250px;" src="https://static.igem.org/mediawiki/2014/c/c5/BioHackspace.Parts.png"><p style="font-size: 0.8em">Fig 1</p></div></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Results</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Results</h2></div></td></tr>
</table>Czechtonhttp://2014.igem.org/wiki/index.php?title=Team:London_BioHackspace/Parts&diff=392192&oldid=prevCzechton at 02:53, 18 October 20142014-10-18T02:53:06Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Parts</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Parts</h2></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><strong><a href="http://parts.igem.org/Part:BBa_K1337000">BBa_K1337000</a></strong><del class="diffchange diffchange-inline">&lt;</del>/br<del class="diffchange diffchange-inline">&gt;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><strong><a href="http://parts.igem.org/Part:BBa_K1337000">BBa_K1337000</a></strong><ins class="diffchange diffchange-inline"><</ins>/br<ins class="diffchange diffchange-inline">></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><em>ompR</em> from <em>E.coli</em></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><em>ompR</em> from <em>E.coli</em></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><strong><a href="http://parts.igem.org/Part:BBa_K1337001">BBa_K1337001</a></strong><del class="diffchange diffchange-inline">&lt;</del>/br<del class="diffchange diffchange-inline">&gt;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><strong><a href="http://parts.igem.org/Part:BBa_K1337001">BBa_K1337001</a></strong><ins class="diffchange diffchange-inline"><</ins>/br<ins class="diffchange diffchange-inline">></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><em>dgc1</em> from <em>G. hansenii</em></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><em>dgc1</em> from <em>G. hansenii</em></p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><strong><a href="http://parts.igem.org/Part:BBa_K1337002">BBa_K1337002</a></strong><del class="diffchange diffchange-inline">&lt;</del>/br<del class="diffchange diffchange-inline">&gt;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><strong><a href="http://parts.igem.org/Part:BBa_K1337002">BBa_K1337002</a></strong><ins class="diffchange diffchange-inline"><</ins>/br<ins class="diffchange diffchange-inline">></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Cph8 signal transduction pathway compatibility tester.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Cph8 signal transduction pathway compatibility tester.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><strong><a href="http://parts.igem.org/Part:BBa_K1337003">BBa_K1337003</a></strong><del class="diffchange diffchange-inline">&lt;</del>/br<del class="diffchange diffchange-inline">&gt;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><strong><a href="http://parts.igem.org/Part:BBa_K1337003">BBa_K1337003</a></strong><ins class="diffchange diffchange-inline"><</ins>/br<ins class="diffchange diffchange-inline">></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Light mediated <em>dgc1</em> transcription construct (should produce the cellular pathway in Figure 1.)</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Light mediated <em>dgc1</em> transcription construct (should produce the cellular pathway in Figure 1.)</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Czechtonhttp://2014.igem.org/wiki/index.php?title=Team:London_BioHackspace/Parts&diff=392035&oldid=prevCzechton at 02:51, 18 October 20142014-10-18T02:51:56Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Parts</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Parts</h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><del class="diffchange diffchange-inline">**[BBa</del><<del class="diffchange diffchange-inline">em</del>><del class="diffchange diffchange-inline">K1337000](</del>parts.igem.org/Part:<del class="diffchange diffchange-inline">BBa</del></<del class="diffchange diffchange-inline">em</del>><del class="diffchange diffchange-inline">K1337000)**</del>&lt;/br&gt;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><<ins class="diffchange diffchange-inline">strong</ins>><ins class="diffchange diffchange-inline"><a href="http://</ins>parts.igem.org/Part:<ins class="diffchange diffchange-inline">BBa_K1337000">BBa_K1337000</a></ins></<ins class="diffchange diffchange-inline">strong</ins>>&lt;/br&gt;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><em>ompR</em> from <em>E.coli</em></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><em>ompR</em> from <em>E.coli</em></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><del class="diffchange diffchange-inline">**[BBa</del><<del class="diffchange diffchange-inline">em</del>><del class="diffchange diffchange-inline">K1337001](</del>parts.igem.org/Part:<del class="diffchange diffchange-inline">BBa</del></<del class="diffchange diffchange-inline">em</del>><del class="diffchange diffchange-inline">K1337001)**</del>&lt;/br&gt;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><<ins class="diffchange diffchange-inline">strong</ins>><ins class="diffchange diffchange-inline"><a href="http://</ins>parts.igem.org/Part:<ins class="diffchange diffchange-inline">BBa_K1337001">BBa_K1337001</a></ins></<ins class="diffchange diffchange-inline">strong</ins>>&lt;/br&gt;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><em>dgc1</em> from <em>G. hansenii</em></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><em>dgc1</em> from <em>G. hansenii</em></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><del class="diffchange diffchange-inline">**[BBa</del><<del class="diffchange diffchange-inline">em</del>><del class="diffchange diffchange-inline">K1337002](</del>parts.igem.org/Part:<del class="diffchange diffchange-inline">BBa</del></<del class="diffchange diffchange-inline">em</del>><del class="diffchange diffchange-inline">K1337002)**</del>&lt;/br&gt;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><<ins class="diffchange diffchange-inline">strong</ins>><ins class="diffchange diffchange-inline"><a href="http://</ins>parts.igem.org/Part:<ins class="diffchange diffchange-inline">BBa_K1337002">BBa_K1337002</a></ins></<ins class="diffchange diffchange-inline">strong</ins>>&lt;/br&gt;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Cph8 signal transduction pathway compatibility tester.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Cph8 signal transduction pathway compatibility tester.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><del class="diffchange diffchange-inline">**[BBa</del><<del class="diffchange diffchange-inline">em</del>><del class="diffchange diffchange-inline">K1337003](</del>parts.igem.org/Part:<del class="diffchange diffchange-inline">BBa</del></<del class="diffchange diffchange-inline">em</del>><del class="diffchange diffchange-inline">K1337003)**</del>&lt;/br&gt;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><<ins class="diffchange diffchange-inline">strong</ins>><ins class="diffchange diffchange-inline"><a href="http://</ins>parts.igem.org/Part:<ins class="diffchange diffchange-inline">BBa_K1337003">BBa_K1337003</a></ins></<ins class="diffchange diffchange-inline">strong</ins>>&lt;/br&gt;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Light mediated <em>dgc1</em> transcription construct (should produce the cellular pathway in Figure 1.)</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Light mediated <em>dgc1</em> transcription construct (should produce the cellular pathway in Figure 1.)</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Following on from that, we hope to also produce and submit our other planned plasmids.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Following on from that, we hope to also produce and submit our other planned plasmids.</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><groupparts>iGEM013 London_BioHackspace</groupparts></del></div></td><td colspan="2"> </td></tr>
</table>Czechtonhttp://2014.igem.org/wiki/index.php?title=Team:London_BioHackspace/Parts&diff=391674&oldid=prevCzechton at 02:49, 18 October 20142014-10-18T02:49:01Z<p></p>
<a href="http://2014.igem.org/wiki/index.php?title=Team:London_BioHackspace/Parts&diff=391674&oldid=28365">Show changes</a>Czechtonhttp://2014.igem.org/wiki/index.php?title=Team:London_BioHackspace/Parts&diff=28365&oldid=prevIGEM HQ: Prototype team page2014-07-08T17:55:55Z<p>Prototype team page</p>
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<h1 >WELCOME TO iGEM 2014! </h1><br />
<p>Your team has been approved and you are ready to start the iGEM season!<br />
<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p><br />
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:London_BioHackspace/Parts&action=edit"style="color:#FFFFFF"> Click here to edit this page!</a> </p><br />
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<a href="https://2014.igem.org/Team:London_BioHackspace"style="color:#000000">Home </a> </td><br />
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<a href="https://2014.igem.org/Team:London_BioHackspace/Team"style="color:#000000"> Team </a> </td><br />
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<a href="https://igem.org/Team.cgi?year=2014&team_name=London_BioHackspace"style="color:#000000"> Official Team Profile </a></td><br />
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<a href="https://2014.igem.org/Team:London_BioHackspace/Project"style="color:#000000"> Project</a></td><br />
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<a href="https://2014.igem.org/Team:London_BioHackspace/Parts"style="color:#000000"> Parts</a></td><br />
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<a href="https://2014.igem.org/Team:London_BioHackspace/Modeling"style="color:#000000"> Modeling</a></td><br />
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<a href="https://2014.igem.org/Team:London_BioHackspace/Notebook"style="color:#000000"> Notebook</a></td><br />
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<a href="https://2014.igem.org/Team:London_BioHackspace/Attributions"style="color:#000000"> Attributions </a></td><br />
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td><br />
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<tr><td > <h3> Parts Submitted to the Registry </h3></td><br />
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<td > <h3>What information do I need to start putting my parts on the Registry? </h3></td><br />
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<p><br />
An important aspect of the iGEM competition is the use and creation of standard biological parts. Each team will make new parts during iGEM and will submit them to the <a href="http://partsregistry.org"> Registry of Standard Biological Parts</a>. The iGEM software provides an easy way to present the parts your team has created. The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox. <br />
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<strong>Note that if you want to document a part you need to document it on the <a href="http://partsregistry.org Registry"> Registry</a>, not on your team wiki.</strong> Future teams and other users and are much more likely to find parts on the Registry than on your team wiki. <br />
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Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without a need to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.<br />
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<h3>When should you put parts into the Registry?</h3><br />
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As soon as possible! We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better recall you will have of all details surrounding your parts. Remember you don't need to send us the DNA to create an entry for a part on the Registry. However, you must send us the sample/DNA before the Jamboree. Only parts for which you have sent us samples/DNA are eligible for awards and medal requirements. <br />
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The information needed to initially create a part on the Registry is:<br />
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<li>Part Name</li><br />
<li>Part type</li><br />
<li>Creator</li><br />
<li>Sequence</li><br />
<li>Short Description (60 characters on what the DNA does)</li><br />
<li>Long Description (Longer description of what the DNA does)</li><br />
<li>Design considerations</li><br />
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. Check out part <a href="http://parts.igem.org/Part:BBa_K404003">BBa_K404003</a> for an excellent example of a highly characterized part. <br />
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You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry"> Add a Part to the Registry</a> link.<br />
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<tr><td colspan="3" > <h3> Parts Table</h3></td></tr><br />
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Any parts your team has created will appear in this table below:</td></tr><br />
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<groupparts>iGEM013 London_BioHackspace</groupparts></div>IGEM HQ