Team:LZU-China/wetlab2

From 2014.igem.org

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/*This is wetlab2*/
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<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
 +
<html xmlns=" http://www.w3.org/1999/xhtml">
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<head>
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<meta http-equiv="Content-Type" content="text/html; charset=gb2312" />
 +
<title>LZU-China 2014</title>
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<style type="text/css">
 +
 
 +
 
 +
 
 +
 
 +
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {display: none;} /*-- hides default wiki settings --*/
 +
 
 +
.firstHeading {
 +
width: 975px;
 +
margin: 0px auto;
 +
padding-top: 100px;
 +
margin-bottom: 20px;
 +
font-family: Georgia, Times, "Times New Roman", serif;
 +
}
 +
 
 +
h1, h2, h3, h4, h5 { font-family: Georgia, Times, "Times New Roman", serif;}
 +
 
 +
#top-section { /*-- styling for default menu bar (edit, page, history, etc.) --*/
 +
background-color: #383838;
 +
border: 0 none;
 +
height: 14px;
 +
z-index: 100;
 +
top: 0;
 +
position: fixed;
 +
width: 975px;
 +
left: 50%;
 +
margin-left: -487px;
 +
}
 +
 
 +
#top-section-bar { /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/
 +
background-color: #383838;
 +
height: 14px;
 +
display: block;
 +
z-index: 10;
 +
position: fixed;
 +
width: 100%;
 +
top: 0;
 +
}
 +
 
 +
#menubar a:link, #menubar a:active, #menubar a:visited, #menubar a:hover, #menubar:hover { /*-- styling for default menu bar links (edit, page, history, etc.) --*/
 +
color: #727272;
 +
text-decoration: none;
 +
background-color: transparent;
 +
}
 +
 
 +
body {
 +
background-color: #fff;
 +
}
 +
 
 +
#globalWrapper, #content { /*-- changes default wiki settings --*/
 +
width: 100%;
 +
height: 100%;
 +
border: 0px;
 +
background-color: transparent;
 +
margin: 0px;
 +
padding: 0px;
 +
}
 +
 
 +
html, body, .wrapper { /*-- changes default wiki settings --*/
 +
width: 100%;
 +
height: 100%;
 +
background-color: transparent;
 +
}
 +
 
 +
#contentcontainer { /*-- creates container for all content on page --*/
 +
font-family: Arial, Helvetica, sans-serif;
 +
font-weight: normal;
 +
font-size: 14px;
 +
color: #414141;
 +
width: 960px;
 +
margin-left: auto;
 +
margin-right: auto;
 +
background-color: #transparent;
 +
margin-top: 0px;
 +
}
 +
 
 +
.sidemenu, .sidemenu li {
 +
list-style-type: none;
 +
list-style-image: none;
 +
font-family: verdana;
 +
text-decoration: none;
 +
color:#000;
 +
font-size: 14px;
 +
}
 +
 
 +
.sidemenu li {display: block;}
 +
 
 +
.sidemenu a {
 +
text-decoration:none;
 +
color: #000;
 +
display: block;
 +
width: 180px;
 +
height: 100%;
 +
padding: 3px 5px;
 +
transition: .25s ease-in-out;
 +
-moz-transition: .25s ease-in-out;
 +
-webkit-transition: .25s ease-in-out;
 +
}
 +
 
 +
.sidemenu a.greyout { color: #909090; }
 +
 
 +
.sidemenu a:hover {
 +
color:#666;
 +
background-color: #d9f5aa;
 +
}
 +
 
 +
.sub-sidemenu {font-size: 10px; margin-bottom: 20px; color: #666; display: none;}
 +
.sub-sidemenu a {color: #666;}
 +
 
 +
div.underconst
 +
{
 +
padding:10px;
 +
border-radius:5px;
 +
background-color:pink;
 +
text-align:center;
 +
}
 +
 
 +
a.anchor{display: block; position: relative; top: -80px; visibility: hidden;}
 +
 
 +
.contentpara {margin-bottom: 30px;}
 +
 
 +
span.email{font-family: monospace; font-weight: normal;}
 +
 
 +
fieldset {border: 1px solid #337f53;}
 +
 
 +
.greyout {color: #A0A0A0;}
 +
.highlightme {background-color: #FFFF00;}
 +
 
 +
#alertContainer {border: 2px solid red; margin-bottom: 10px; width: 936px;}
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#annContainer {margin-left: 8px;}
 +
#newsContainer {border: 1px solid #ccc;}
 +
 
 +
.newsTitle {
 +
display: block;
 +
color: #414141;
 +
font-size: 25px; 
 +
        font-family: Georgia, Times, "Times New Roman", serif;
 +
padding: 10px 15px 5px 10px;
 +
border-bottom: 1px solid #ccc;
 +
margin-bottom: 0px;
 +
}
 +
 
 +
.newsItem {
 +
border-bottom: 1px solid #ccc;
 +
display: block;
 +
padding: 5px 15px 0px 10px;
 +
margin-bottom: 0px;
 +
}
 +
 
 +
.newsItem h3 {
 +
width: auto;
 +
display: inline;
 +
font-size: 14px;
 +
font-family: Arial, Helvetica, sans-serif;
 +
padding: 0px;
 +
margin: 0px;
 +
}
 +
 
 +
.newsItem img {
 +
float: right;
 +
clear: right;
 +
width: 80px;
 +
padding-left: 15px;
 +
display: inline-block;
 +
}
 +
 
 +
.newsItem .newsDate {
 +
font-style: italic;
 +
font-size: 14px;
 +
display: inline-block;
 +
color: #999;
 +
float: right;
 +
padding: 0px 0px 10px 15px;
 +
}
 +
 
 +
.newsItem p, .newsItem ul, .newsItem li {
 +
padding: 0px;
 +
margin-left: 20px;
 +
font-size: 12px;
 +
line-height: 1.2;
 +
}
 +
 
 +
.newsItem p {margin-top: 10px;}
 +
 
 +
.annItem {
 +
border: 2px solid #49b677;
 +
display: block;
 +
padding: 0px 15px 10px 10px;
 +
margin-bottom: 18px;
 +
position: relative;
 +
height: 160px;
 +
vertical-align: middle;
 +
}
 +
 
 +
.annItem .annCentered {
 +
display: table-cell;
 +
vertical-align: middle;
 +
height: 160px;
 +
}
 +
 
 +
.annCentered h3 {
 +
padding: 0px;
 +
display: block;
 +
margin-left: auto;
 +
margin-right: auto;
 +
text-align: center;
 +
}
 +
 
 +
.annItem ul, .annItem li {padding: 0px; margin: 0px;}
 +
.annItem li {list-style: none; margin: 5px}
 +
 
 +
@charset "utf-8";
 +
body,h1,h2,h3,h4,h5,h6,hr,p,blockquote,dl,dt,dd,ul,ol,li,pre,form,fieldset,legend,button,input,textarea,th,td,figure{
 +
margin:0;
 +
padding:0;
 +
font-size: 14px;
 +
text-align: left;
 +
}
 +
html{background-color:#e9ecec}
 +
table{table-layout:fixed;border-collapse:collapse}
 +
section,article,aside,header,footer,nav,dialog,figure{display:block}
 +
.clearfix:after{content:"\20";display:block;height:0;line-height:0;font-size:0;clear:both}
 +
body,button,input,select,textarea,td,th{color:#333;font:12px/1.5 tahoma,arial,\5b8b\4f53}
 +
h1,h2,h3,h4,h5,h6{font-size:100%}
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address,cite,dfn,em,var{font-style:normal}
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code,kbd,pre,samp{font-family:courier new,courier,monospace}
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small{font-size:12px}ul,ol{list-style:none}a{text-decoration:none;color:#06C}
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a:hover{text-decoration:underline;color:#06C}
 +
sup{vertical-align:text-top}
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sub{vertical-align:text-bottom}
 +
legend{color:#000}
 +
fieldset,img{border:0 none}
 +
button,input,select,textarea{font-size:100%}
 +
table{
 +
border-collapse:collapse;
 +
border-spacing:0;
 +
text-align: center;
 +
}
 +
input:focus{outline:none}
 +
.hd-main{height:60px;background:#fff;z-index:15;border-bottom:#b3b3b3 1px solid;box-shadow:0 0 5px #333}
 +
.f-icon{background-image:url('http://www.codefans.net/jscss/demoimg/201403//frame-icon.png');background-repeat:no-repeat}
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.hd-main{padding:0 5px;height:49px;min-width:800px;background:#252525;border-bottom:0}
 +
.hd-main .has-pulldown{cursor:pointer;position:relative;*z-index:10000}
 +
.hd-main .pulldown{position:absolute;cursor:default;display:none;top:30px;left:0}
 +
.hd-main .pulldown .arrow{*margin-bottom:-1px;_margin-bottom:0;height:0;width:0;font-size:0;line-height:0;border-width:7px;border-style:solid;border-color:transparent transparent rgba(255, 255, 255,0.8) transparent;_filter:chroma(color=tomato);_border-color:tomato tomato rgba(255, 255, 255,0.8) tomato;position:relative;display:block;left:20px;z-index:7}
 +
.hd-main .pulldown .content{background:rgba(255, 255, 255,0.8);color:#333;text-align:left;border-radius:3px;border:rgb(175,175,175) 1px solid;border-width:0 1px 1px 1px;box-shadow:0 2px 4px rgba(0,0,0,.2);position:relative;z-index:6}
 +
.hd-main .pulldown-canvas{position:absolute;top:0;left:0;width:100%;height:100%;opacity:0;filter:alpha(opacity=0)}
 +
.hd-main .logo{background-image:url('http://2014.igem.org/wiki/images/7/72/2014logomin.png');background-repeat:no-repeat;display:block;height:36px;width:175px;float:left;margin-left:10px;background-position:0 0;margin-top:5px;display:inline}
 +
.hd-main .navs{padding:0 0 3px 25px;height:45px;float:left}
 +
.hd-main .navs a,.hd-main .info a,.hd-main .navs a:visited,.hd-main .info a:visited,.hd-main .navs a:active,.hd-main .info a:active{color:#d8d8d8}
 +
.hd-main .navs a:hover,.hd-main .navs a:active{text-decoration:none;background-color:#0f0f0f}
 +
.hd-main .navs .def-nav,.hd-main .navs .pulldown-nav,.hd-main .navs .separate{display:block;float:left;height:48px;font:18px/30px "Microsoft YaHei","Microsoft JhengHei";color:#d8d8d8;text-align:center;width:125px;line-height:48px}
 +
.hd-main .navs .def-nav .app-url{left:185px;width:425px;top:48px}
 +
.hd-main .navs .def-nav .pulldown-canvas-url{width:575px;height:109px}
 +
.hd-main .navs .def-nav .app-url .arrow{left:140px}
 +
.hd-main .navs .app-url .content{padding:17px 0 0 22px;height:91px}
 +
.hd-main .navs .app-url .content a,.hd-main .navs .app-url .content a:hover{color:#333}
 +
.hd-main .navs .app-url .li{cursor:pointer;padding-top:8px;width:66px;height:66px;border-radius:3px;display:block;float:left;text-align:center}
 +
.hd-main .navs .app-url .li:hover{background-color:#e0e1e5}
 +
.hd-main .navs .app-url .li .icon{margin:0 auto;width:50px;height:50px;display:block;background:url('http://2014.igem.org/wiki/images/9/94/Icons50X50nb.png') no-repeat}
 +
.hd-main .navs .app-url .li:hover .icon{background-image:url('http://2014.igem.org/wiki/images/9/94/Icons50X50nb.png')}
 +
.hd-main .navs .app-url .tongxunlu .icon{background-position:-50px 0}
 +
.hd-main .navs .app-url .xiangce .icon{background-position:-100px 0}
 +
.hd-main .navs .app-url .note .icon{background-position:-150px 0}
 +
.hd-main .navs .app-url .duanxin .icon{background-position:-200px 0}
 +
.hd-main .navs .app-url .zhaohui .icon{background-position:-250px 0}
 +
.hd-main .navs .app-url .youxi .icon{background-position:-300px 0}
 +
.hd-main .navs .app-url .li .text{height:30px;display:block;font:10px/30px "Microsoft YaHei","Microsoft JhengHei"}
 +
.hd-main .navs .separate{margin:0;height:48px;width:2px;background:#2A2B2D}
 +
.hd-main .navs .pulldown-nav{position:relative}
 +
.hd-main .navs .pulldown-nav em{
 +
position:absolute;
 +
display:block;
 +
left:99px;
 +
top:18px;
 +
height:12px;
 +
width:12px;
 +
font-size:0;
 +
background-position:-89px -45px
 +
}
 +
.hd-main .navs .pulldown-nav:hover .f-icon,.hd-main .navs .pulldown-nav:active .f-icon{position:absolute;display:block;right:11px;top:18px;height:12px;width:12px;font-size:0;background-position:-89px -55px}
 +
.hd-main .navs .current,.hd-main .navs .current .pulldown-nav{font-weight:bold;color:#FFF;display:inline-block;width:125px;height:49px;background-color:#0f0f0f;text-align:center;line-height:48px}
 +
.hd-main .navs .current:hover{color:#ECECEC}
 +
.hd-main .navs .current .hover{background-color:#dedede;color:#333;}
 +
.hd-main .info,.hd-main .info ul{height:36px;float:right;text-align:right;margin-top:3px;white-space:nowrap}
 +
.hd-main .info a{color:#d8d8d8}
 +
.hd-main .info a:hover{color:#FFF;text-decoration:underline}
 +
.hd-main .info .info-i{padding:0 10px;margin:10px 0;display:inline-block;*display:inline;*zoom:1;height:16px;border-right:#393939 1px solid;color:#d8d8d8;font:12px/16px "SimSun"}
 +
.hd-main{padding:0 5px;height:49px;min-width:800px;background:#252525;border-bottom:0}
 +
.hd-main .info .has-pulldown{padding-right:28px}
 +
.hd-main .info .has-pulldown .pull-arrow{position:absolute;display:block;right:10px;top:1px;height:12px;width:12px;font-size:0;background-position:-89px -45px}
 +
.hd-main .info .no-separate{border:none}
 +
.hd-main .info .user-name{position:relative;padding-left:24px}
 +
.hd-main .info .user-name .name{display:inline-block;display:inline\0;white-space:nowrap;max-width:75px;overflow:hidden;text-overflow:ellipsis;vertical-align:bottom}
 +
.hd-main .info .user-name .identity{position:absolute;top:0;left:0;height:16px;width:16px;background-position:-89px -88px}
 +
.hd-main .info .user-name .is-vip{background-position:-89px -68px}
 +
.hd-main .info .user-info,.hd-main .info .more-info{top:9px;left:auto;right:-24px;width:170px} /*User栏目宽度*/
 +
.hd-main .info .notice-nu{display:inline-block;padding-left:4px;background-position:-86px -109px;height:14px;-webkit-text-size-adjust:none;font-size:10px;font-style:normal}
 +
.hd-main .info .notice-nu .f-icon{display:inline-block;padding-right:4px;background-position:right -109px;color:#FFF}  /*此处原为#FFF*/
 +
.hd-main .info .user-info .arrow{left:70px;*left:-32px}
 +
.hd-main .info .user-info .content,.hd-main .info .more-info .content{padding:10px 0;height:180px}
 +
.hd-main .info .more{padding:0;display:inline;line-height:inherit;background:none}
 +
.hd-main .info .more-info .content{height:150px}
 +
.hd-main .info .user-info .content a,.hd-main .info .more-info .content a{display:block;padding-left:33px;height:30px;line-height:30px;color:#888}
 +
.hd-main .info .user-info .content a:hover,.hd-main .info .more-info .content a:hover{color:#333;text-decoration:none;background:#e0e1e5}
 +
.hd-main .info .user-info .li,.hd-main .info .more-info .li{display:block;padding:2px 0;height:30px}
 +
.hd-main .info .user-info .separate-li,.hd-main .info .more-info .separate-li{display:block;margin:3px 11px;border-top:#EBEBEC 1px solid;border-bottom:#FFF 1px solid}
 +
.hd-main .info .more-info{right:0}
 +
.hd-main .info .more-info .arrow{left:94px;*left:-8px}
 +
#apDiv1 {
 +
position:absolute;
 +
left:3px;
 +
top:4px;
 +
width:100%;
 +
height:900px;
 +
z-index:0;
 +
background-color: #66CCFF;
 +
}
 +
/*<![CDATA[*/
 +
body {
 +
margin:0;
 +
padding:0 10px 0 10px;
 +
border:0;
 +
height:100%;
 +
overflow:auto;
 +
background-color: #FFF;
 +
}
 +
#menu {right:0; top:0; width:100%; position:fixed; padding:10px; text-align:center; font-weight:bold; background:rgba(0,83,163,0);}
 +
* html #menu {position:absolute; right:16px; top:0;}/*only for ie*/
 +
html {overflow:auto !important; overflow:hidden;}
 +
 
 +
#apDiv2 {
 +
position:absolute;
 +
left:0;
 +
top:50px;
 +
width:100%;
 +
height:265px;
 +
z-index:0;
 +
background-color: #187BDB;
 +
}
 +
#apDiv3 {
 +
position:absolute;
 +
left:0px;
 +
top:318px;
 +
width:100%;
 +
height:150px;
 +
z-index:0;
 +
}
 +
#apDiv3 table tr td p {
 +
font-size: 15px;
 +
text-align: left;
 +
}
 +
#apDiv4 {
 +
position:absolute;
 +
left:0;
 +
top:604px;
 +
width:100%;
 +
height:1396px;
 +
z-index:0;
 +
}
 +
#apDiv5 {
 +
position:absolute;
 +
left:0;
 +
top:4588px;
 +
width:100%;
 +
height:25;
 +
z-index:1;
 +
background-color: #5FC1C5;
 +
}
 +
#apDiv3 div {
 +
font-size: 15px;
 +
text-align: center;
 +
}
 +
#apDiv4 table tr td div {
 +
font-size: 45px;
 +
color: #000;
 +
text-align: left;
 +
}
 +
#apDiv4 table tr td table tr td {
 +
font-size: 16px;
 +
line-height: 30px;
 +
}
 +
body,td,th {
 +
color: #000;
 +
}
 +
#apDiv6 {
 +
position:absolute;
 +
left:575px;
 +
top:4477px;
 +
width:220px;
 +
height:221px;
 +
z-index:2;
 +
}
 +
#apDiv2 table tr td p {
 +
font-size: 45px;
 +
text-align: center;
 +
color: #FFF;
 +
font-weight: bold;
 +
}
 +
#apDiv7 {
 +
position:absolute;
 +
left:50%;
 +
top:226px;
 +
width:257px;
 +
height:151px;
 +
z-index:auto;
 +
}
 +
#apDiv3 table tr td {
 +
font-size: 15px;
 +
}
 +
#apDiv4 table tr td table tr td p {
 +
font-size: 15px;
 +
line-height: 30px;
 +
}
 +
#apDiv8 {
 +
position:absolute;
 +
left:0;
 +
top:1900px;
 +
width:100%;
 +
height:1430px;
 +
z-index:0;
 +
}
 +
#apDiv8 div p {
 +
font-size: 45px;
 +
}
 +
#apDiv9 {
 +
position:absolute;
 +
left:0;
 +
top:3431px;
 +
width:100%;
 +
height:213px;
 +
z-index:0;
 +
}
 +
#apDiv9 div p {
 +
font-size: 45px;
 +
}
 +
#apDiv9 table tr td p {
 +
font-size: 15px;
 +
line-height: 30px;
 +
}
 +
#apDiv8 table tr td p {
 +
font-size: 15px;
 +
line-height: 30px;
 +
}
 +
#apDiv9 table tr td div {
 +
font-size: 45px;
 +
}
 +
#apDiv8 table tr td div {
 +
font-size: 45px;
 +
}
 +
</style>
 +
</head>
 +
 
 +
<body id="" class="">
 +
<div id="apDiv2">
 +
  <table width="100%" border="0" >
 +
    <tr>
 +
      <td width="1201" height="265" rowspan="2"><p><img src="http://2014.igem.org/wiki/images/thumb/8/84/2.wetlab.top.jpg/800px-2.wetlab.top.jpg" width="1366" height="270" /></p></td>
 +
      <td width="100%" height="199" bgcolor="#187ADB">&nbsp;</td>
 +
    </tr>
 +
    <tr>
 +
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  <table width="100%" border="0">
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      <td width="18%">&nbsp;</td>
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      <td width="64%"><p>&nbsp;</p>
 +
        <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We constructed  a pollutant substrate(PNP) bio-sensor coupling riboflavin synthetic gene cluster,  the genetically modified E.coli can secrete riboflavin when added PNP in MFC anode medium.Riboflavin is a efficient redox mediator as well as a stimulator of MFC. We created a novel MFC devices and built a quantitative monitor system of PNP via measuring voltage increment.</p>
 +
        <p>&nbsp;</p>
 +
        <p>&nbsp;</p>
 +
<p><a href="http://2014.igem.org/Team:LZU-China/wetlab"><img src="http://2014.igem.org/wiki/images/4/40/Dht1_%281%29.jpg" width="238" height="75" /></a><img src="http://2014.igem.org/wiki/images/2/2c/Dht1_(2).jpg" width="229" height="75" /><img src="http://2014.igem.org/wiki/images/e/e8/Dht1_(3).jpg" width="204" height="75" /><a href="http://2014.igem.org/Team:LZU-China/wetlab4"><img src="http://2014.igem.org/wiki/images/0/02/Dht1_(4).jpg" width="299" height="75" /></a></p></td>
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      <td width="18%">&nbsp;</td>
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    </tr>
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  </table>
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  <p>&nbsp;</p>
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  <p></p>
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 +
      <p>&nbsp;</p>
 +
      <div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;GENETIC ENGINEERED BACTERIA</div>
 +
      <table width="100%" border="0">
 +
        <tr>
 +
          <td background="http://2014.igem.org/wiki/images/a/a6/Huils.jpg" width="30%" height="1146"><p>&nbsp;</p></td>
 +
          <td background="http://2014.igem.org/wiki/images/a/a6/Huils.jpg" width="57%">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We designed this pathway for our purpose. As you can see, the NsrR is constitutive expressed in almost all E. coli orgnisms and it can repress the promoter PyeaR. In our project we found that this sensitive system is also adjusted to the PNP(p-nitrophenol). So we constructed this sensor pathway.</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The sensor will cause the expression of riboflavin, a kind of mediator which can induce the generation of current in MFCs, to show the appearance of PNP. We can estimate the concentration of PNP. </p>
 +
            <p><img src="http://2014.igem.org/wiki/images/e/ee/WETAimage001.png" width="800" height="467" /></p>
 +
            <p align="center">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
 +
            <p align="center">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Figure-1  The pathway of PNP sensor</p>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Besides, we find many  genes can catalyze the reduction of Cr(VI). They can be used in the cathode to  reduce these heavy metal ions.</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="http://2014.igem.org/wiki/images/3/39/WETAimage002.png" width="556" height="307" /></p>
 +
            <p align="center">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Figure-2  The pathway of the Cr ion reductase(yieF as an example)</p></td>
 +
          <td background="http://2014.igem.org/wiki/images/a/a6/Huils.jpg" width="13%">&nbsp;</td>
 +
        </tr>
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      </table>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p></td>
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    </tr>
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  </table>
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</div>
 +
<div id="apDiv5">
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  <table width="1365" border="0"">
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      <td background="http://2014.igem.org/wiki/images/a/a6/Huils.jpg" width="1365" height="463"><img src="http://2014.igem.org/wiki/images/0/07/Dblbluebar.jpg" width="1365" height="463" /></td>
 +
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  </table>
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</div>
 +
<div id="apDiv6"><img src="http://2014.igem.org/wiki/images/6/6e/Lzulogo.png" width="220" height="221" /></div>
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<div id="apDiv8">
 +
  <p>&nbsp;</p>
 +
  <table width="100%" border="0">
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    <tr>
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      <td ><div>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;ABOUT THE CONSTRUCTION OF PNP SENSOR</div></td>
 +
    </tr>
 +
  </table>
 +
  <table width="100%" border="0">
 +
    <tr>
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      <td  width="30%">&nbsp;</td>
 +
      <td  ><p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;        </p>
 +
        <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We found that the part BBa_K381001 by  iGEM10_BCCS-Bristol is a sensor which can detect the appearance of nitrate and  nitrite. So we perfomed an experience by this part to see if this part can  detect PNP(p-Nitrophenol).
 +
          We got a good result.You can see the PNP  can also induce the green fluorescence.</p>
 +
      <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="http://2014.igem.org/wiki/images/3/3c/Image005.png" width="455" height="340" /></p>
 +
      <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Figure-3 Fluorescence  of different system. </p>
 +
      <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;a.bacterial liquid with 10mM PNP;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;b.bacterial liquid with  ddH2O;</p>
 +
      <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;c.bacterial liquid with 10mM KNO3;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;d.bacterial  liquid with 10mM KCl.</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Firstly we got the plasmid with K1172303  from Registry. We cut the plasmid by EcoRI and XbaI, then put the <em>Pnsr</em>(K216005+B0030  this sequence was synthesized by company)  into the gap.</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="http://2014.igem.org/wiki/images/0/06/Image008.jpg" width="489" height="90" /></p>
 +
      <p align="center">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Figure-4  Construction of K1523101</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The size of the whole part is about  3700bp(without plasmid), we test the assembling result by PCR(sense:5&rsquo;---TTCCCATCTATAATCCTCCCTGATTCTTCG---3&rsquo;;anti-sense:5&rsquo;---GAATTCTCTAGATTACAACTGTTGTTCAAGCTGTT---3&rsquo;).  From the gel picture we can see the size is right.</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="http://2014.igem.org/wiki/images/c/c1/Image009.png" width="643" height="305" /></p>
 +
      <p align="center">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Figure-5  Gel picture of K1523101&rsquo;s PCR</p>
 +
      <p align="center">&nbsp;</p></td>
 +
      <td  width="13%">&nbsp;</td>
 +
    </tr>
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  </table>
 +
  <p>&nbsp;</p>
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</div>
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<div id="apDiv9">
 +
  <p>&nbsp;</p>
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  <table width="100%" border="0" >
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    <tr>
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      <td background="http://2014.igem.org/wiki/images/a/a6/Huils.jpg"><p>&nbsp;</p>
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    </tr>
 +
    <tr>
 +
      <td background="http://2014.igem.org/wiki/images/a/a6/Huils.jpg" ><div>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;ABOUT THE REDUCING Cr IONS GENES</div></td>
 +
    </tr>
 +
  </table>
 +
  <div></div>
 +
  <table width="100%" border="0" >
 +
    <tr>
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      <td background="http://2014.igem.org/wiki/images/a/a6/Huils.jpg" width="30%">&nbsp;</td>
 +
      <td background="http://2014.igem.org/wiki/images/a/a6/Huils.jpg" width="57%"><p align="left">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
 +
        <p align="left">&nbsp;</p>
 +
        <p align="left">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We found  four genes to reduce the Cr(VI) to Cr(III). They are<em> nahG, nahR, nahE, chrR</em> and <em>yieF. </em>We didn&rsquo;t do the deep research about them because of time  limitation. They can be used in the general plasmids such as pBR322. We just  made them to be the formation of Biobrick. More uses and functions need to be  demonstrated and found.</p>
 +
        <p align="left"><strong><em>nahG</em></strong>: We got the sequence from <em>Pseudomonas putida</em> </p>
 +
        <p align="left">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(Primer:sense:5&rsquo;---CCGGAATTCGCGGCCGCTTCTAGATGAAAAACAATAAACCTGGCTTGCGC---3&rsquo;;<br />
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;anti-sense:5&rsquo;---AAAATACTAGTAGCGGCCGCTGCAGTCACCCTTGACGTAGCACACC---3&rsquo;)<br />
 +
  <strong><em>nahE: </em></strong>We got the sequence from <em>Pseudomonas putida<strong> </strong></em><br />
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(Primer:sense:5&rsquo;---CCGGAATTCGCGGCCGCTTCTAGATGTCGAATAAAATTATGAAAACGTCGCG---3&rsquo;;<br />
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;anti-sense:5&rsquo;---AAAATACTAGTAGCGGCCGCTGCAGCTACTTCAATTCATTACTGTATTTAGCGTG---3&rsquo;)<br />
 +
  <strong><em>nahR: </em></strong>We got the sequence from <em>Pseudomonas putida<strong> </strong></em><br />
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(Primer:sense:5&rsquo;---  CCGGAATTCGCGGCCGCTTCTAGATGAAAAACAATAAACCTGGCTTGCGC---3&rsquo;;<br />
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;anti-sense:5&rsquo;---AAAATACTAGTAGCGGCCGCTGCAGTCACCCTTGACGTAGCACACC---3&rsquo;)<br />
 +
  <strong><em>chrR: </em></strong>We got the sequence from <em>Escherichia coli str.  K-12<strong> </strong></em><br />
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(Primer:sense:5&rsquo;---  CCGGAATTCGCGGCCGCTTCTAGATGAAAAAAATAGTCCAGTCGGAAGG---3&rsquo;;<br />
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;anti-sense:5&rsquo;---AAAATACTAGTAGCGGCCGCTGCAGTCAGGCCTTTTTGTGCTGTTCAAC---3&rsquo;)<br />
 +
  <strong><em>yieF: </em></strong>We got the sequence from <em>Escherichia coli str.  K-12<strong> </strong></em><br />
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(Primer:sense:5&rsquo;---  GAATTCGGATCCATGTCTGAAAAATTGCAGGTGG---3&rsquo;;<br />
 +
        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;anti-sense:5&rsquo;---GAATTCTCTAGATTAGATCTTAACTCGCTGAATAAA---3&rsquo;)</p>
 +
<p align="left">&nbsp;</p>
 +
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Revision as of 19:10, 17 October 2014

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> LZU-China 2014

 
 

 

 

 

         We constructed a pollutant substrate(PNP) bio-sensor coupling riboflavin synthetic gene cluster, the genetically modified E.coli can secrete riboflavin when added PNP in MFC anode medium.Riboflavin is a efficient redox mediator as well as a stimulator of MFC. We created a novel MFC devices and built a quantitative monitor system of PNP via measuring voltage increment.

 

 

 

 

 

 

 

        GENETIC ENGINEERED BACTERIA

 

            

          We designed this pathway for our purpose. As you can see, the NsrR is constitutive expressed in almost all E. coli orgnisms and it can repress the promoter PyeaR. In our project we found that this sensitive system is also adjusted to the PNP(p-nitrophenol). So we constructed this sensor pathway.

          The sensor will cause the expression of riboflavin, a kind of mediator which can induce the generation of current in MFCs, to show the appearance of PNP. We can estimate the concentration of PNP.

          

                                                              Figure-1 The pathway of PNP sensor

 

          Besides, we find many genes can catalyze the reduction of Cr(VI). They can be used in the cathode to reduce these heavy metal ions.

                                        

                                                             Figure-2 The pathway of the Cr ion reductase(yieF as an example)

 

 

 

 

        ABOUT THE CONSTRUCTION OF PNP SENSOR
 

          

          We found that the part BBa_K381001 by iGEM10_BCCS-Bristol is a sensor which can detect the appearance of nitrate and nitrite. So we perfomed an experience by this part to see if this part can detect PNP(p-Nitrophenol). We got a good result.You can see the PNP can also induce the green fluorescence.

                                    

                                                            Figure-3 Fluorescence of different system.

                                        a.bacterial liquid with 10mM PNP;          b.bacterial liquid with ddH2O;

                                        c.bacterial liquid with 10mM KNO3;        d.bacterial liquid with 10mM KCl.

 

          Firstly we got the plasmid with K1172303 from Registry. We cut the plasmid by EcoRI and XbaI, then put the Pnsr(K216005+B0030 this sequence was synthesized by company) into the gap.

 

                                   

                                                            Figure-4 Construction of K1523101

 

          The size of the whole part is about 3700bp(without plasmid), we test the assembling result by PCR(sense:5’---TTCCCATCTATAATCCTCCCTGATTCTTCG---3’;anti-sense:5’---GAATTCTCTAGATTACAACTGTTGTTCAAGCTGTT---3’). From the gel picture we can see the size is right.

 

                               

                                                                            Figure-5 Gel picture of K1523101’s PCR

 

 

 

 

 

 

        ABOUT THE REDUCING Cr IONS GENES
 

         

 

          We found four genes to reduce the Cr(VI) to Cr(III). They are nahG, nahR, nahE, chrR and yieF. We didn’t do the deep research about them because of time limitation. They can be used in the general plasmids such as pBR322. We just made them to be the formation of Biobrick. More uses and functions need to be demonstrated and found.

nahG: We got the sequence from Pseudomonas putida

          (Primer:sense:5’---CCGGAATTCGCGGCCGCTTCTAGATGAAAAACAATAAACCTGGCTTGCGC---3’;
          anti-sense:5’---AAAATACTAGTAGCGGCCGCTGCAGTCACCCTTGACGTAGCACACC---3’)
nahE: We got the sequence from Pseudomonas putida
          (Primer:sense:5’---CCGGAATTCGCGGCCGCTTCTAGATGTCGAATAAAATTATGAAAACGTCGCG---3’;
          anti-sense:5’---AAAATACTAGTAGCGGCCGCTGCAGCTACTTCAATTCATTACTGTATTTAGCGTG---3’)
nahR: We got the sequence from Pseudomonas putida
          (Primer:sense:5’--- CCGGAATTCGCGGCCGCTTCTAGATGAAAAACAATAAACCTGGCTTGCGC---3’;
          anti-sense:5’---AAAATACTAGTAGCGGCCGCTGCAGTCACCCTTGACGTAGCACACC---3’)
chrR: We got the sequence from Escherichia coli str. K-12
          (Primer:sense:5’--- CCGGAATTCGCGGCCGCTTCTAGATGAAAAAAATAGTCCAGTCGGAAGG---3’;
          anti-sense:5’---AAAATACTAGTAGCGGCCGCTGCAGTCAGGCCTTTTTGTGCTGTTCAAC---3’)
yieF: We got the sequence from Escherichia coli str. K-12
          (Primer:sense:5’--- GAATTCGGATCCATGTCTGAAAAATTGCAGGTGG---3’;
          anti-sense:5’---GAATTCTCTAGATTAGATCTTAACTCGCTGAATAAA---3’)