Team:LZU-China/tempfortest

From 2014.igem.org

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      <td><table width="100%" border="0">
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          <td>CONTENTS </td>
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       <td width="12%"><p><img src="icon_mini/2wetlab.png" width="100" height="100" /></p>
       <p>Wet Lab</p></td>
       <p>Wet Lab</p></td>
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          <td height="94">ABSTRACT</td>
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      <td valign="top"><p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Microbial  Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is  an ideal approach to solve both pollution problem and energy crisis. However,  MFCs still have disadvantages such as hard to determine the contaminants  concentrations which is a major drawback for MFCs applications. <br />
 
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        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In  our study, we found that the electricity produced by MFCs was somewhat related  to the substrates concentrations such as P-nitrophenol (PNP) in anode or  Chromium (VI) in cathode. Therefore, we hypothesize that a) by using genetic  engineered bacteria, the MFC&rsquo;s electricity will be more stable and correlated  with substrates concentrations. b) by monitoring MFC&rsquo;s electricity, it will be  possible to measure substrates concentrations. <br />
 
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      &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In this study, we&rsquo;ve designed a novel MFC system. For  anode strain, we&rsquo;ve cloned a PNP sensor sequence and a riboflavin into <em>Escherichia coli</em>. The recombinants is  able to detect PNP and produce riboflavin to boost electrical generation when  co-cultured with <em>Shewanella oneidensis</em>.  In the cathode, gene codes chromate (VI) reductase Yief was cloned into <em>E.coli</em>. The stability of MFCs has been improved  and the electricity generated to correlated with the substrates. Moreover,  based on this correlation, we&rsquo;ve designed a software which is able to  monitor the contaminants concentrations in MFCs. </p>
 
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Revision as of 14:54, 7 October 2014

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> 无标题文档

 

 

 

 

Background

Wet Lab

Dry Lab

Parts

Human Practice

  
 

 

 

 

    

 

    
 

Our team

Interlab

Safety

Notebook

Future Work

  

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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