Team:LZU-China/tempfortest

From 2014.igem.org

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<body id="" class="">
<body id="" class="">
<div id="apDiv2"><img src="https://static.igem.org/mediawiki/2014/thumb/8/8c/Ttfbtmp1.png/800px-Ttfbtmp1.png" width="1366" height="543" /></div>
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<div id="apDiv3">
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  <table width="100%" border="0">
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    <tr>
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      <td><p>&nbsp;</p>
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      <p>&nbsp;</p></td>
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    </tr>
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    <tr>
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      <td><table width="100%" border="0">
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        <tr>
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          <td>CONTENTS </td>
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        </tr>
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      </table></td>
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    </tr>
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    <tr>
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      <td><p>&nbsp;</p>
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    </tr>
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      <td width="20%">&nbsp;</td>
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      <td width="12%"><p><img src="icon_mini/1backgroud.png" width="100" height="100" /></p>
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<p>Background</p></td>
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      <td width="12%"><p><img src="icon_mini/2wetlab.png" width="100" height="100" /></p>
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      <p>Wet Lab</p></td>
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      <td width="12%"><p><img src="icon_mini/3drylab.png" width="100" height="100" /></p>
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      <p>Dry Lab</p></td>
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      <td width="12%"><p><img src="icon_mini/4parts.png" width="100" height="100" /></p>
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      <p>Parts</p></td>
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      <td width="12%"><p><img src="icon_mini/5humanpractics.png" width="100" height="100" /></p>
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      <p>Human Practice</p></td>
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      <td width="20%">&nbsp;</td>
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    </tr>
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    <tr>
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      <td width="20%">&nbsp;</td>
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      <p>&nbsp;</p>
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      <p>&nbsp;</p></td>
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      <td width="12%">&nbsp;</td>
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      <td width="12%">&nbsp;</td>
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      <td width="12%"><p>&nbsp;</p></td>
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      <td width="20%">&nbsp;</td>
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    <tr>
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      <td width="20%">&nbsp;</td>
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      <td width="12%"><p><img src="icon_mini/6members.png" width="100" height="100" /></p>
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      <p>Our team</p></td>
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      <td width="12%"><p><img src="icon_mini/7interlab.png" width="100" height="100" /></p>
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      <p>Interlab</p></td>
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      <td width="12%"><p><img src="icon_mini/8safety.png" width="100" height="100" /></p>
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      <p>Safety</p></td>
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      <p>Notebook</p></td>
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      <td width="12%"><p><img src="icon_mini/10future.png" width="100" height="100" /></p>
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      <p>Future Work</p></td>
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    <tr>
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      <td width="28%" height="104"><table width="100%" border="0">
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        <tr>
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          <td height="94">ABSTRACT</td>
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        </tr>
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      <td width="60%">&nbsp;</td>
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      <td width="12%">&nbsp;</td>
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    <tr>
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      <td height="271">&nbsp;</td>
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      <td valign="top"><p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Microbial  Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is  an ideal approach to solve both pollution problem and energy crisis. However,  MFCs still have disadvantages such as hard to determine the contaminants  concentrations which is a major drawback for MFCs applications. <br />
 +
        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In  our study, we found that the electricity produced by MFCs was somewhat related  to the substrates concentrations such as P-nitrophenol (PNP) in anode or  Chromium (VI) in cathode. Therefore, we hypothesize that a) by using genetic  engineered bacteria, the MFC&rsquo;s electricity will be more stable and correlated  with substrates concentrations. b) by monitoring MFC&rsquo;s electricity, it will be  possible to measure substrates concentrations. <br />
 +
      &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In this study, we&rsquo;ve designed a novel MFC system. For  anode strain, we&rsquo;ve cloned a PNP sensor sequence and a riboflavin into <em>Escherichia coli</em>. The recombinants is  able to detect PNP and produce riboflavin to boost electrical generation when  co-cultured with <em>Shewanella oneidensis</em>.  In the cathode, gene codes chromate (VI) reductase Yief was cloned into <em>E.coli</em>. The stability of MFCs has been improved  and the electricity generated to correlated with the substrates. Moreover,  based on this correlation, we&rsquo;ve designed a software which is able to  monitor the contaminants concentrations in MFCs. </p>
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      <p>&nbsp;</p>
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      <p>&nbsp;</p>
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      <p>&nbsp;</p></td>
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      <td>&nbsp;</td>
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    </tr>
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  <p>&nbsp;</p>
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<p>&nbsp;</p>
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  <p>&nbsp;</p>
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  <p>&nbsp;</p>
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<div style=" height:890px;"> </div>
<div style=" height:890px;"> </div>

Revision as of 14:52, 7 October 2014

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> 无标题文档

 

 
CONTENTS

 

 
 

Background

Wet Lab

Dry Lab

Parts

Human Practice

  
 

 

 

 

    

 

    
 

Our team

Interlab

Safety

Notebook

Future Work

  
ABSTRACT
   
 

              Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs still have disadvantages such as hard to determine the contaminants concentrations which is a major drawback for MFCs applications.
             In our study, we found that the electricity produced by MFCs was somewhat related to the substrates concentrations such as P-nitrophenol (PNP) in anode or Chromium (VI) in cathode. Therefore, we hypothesize that a) by using genetic engineered bacteria, the MFC’s electricity will be more stable and correlated with substrates concentrations. b) by monitoring MFC’s electricity, it will be possible to measure substrates concentrations.
             In this study, we’ve designed a novel MFC system. For anode strain, we’ve cloned a PNP sensor sequence and a riboflavin into Escherichia coli. The recombinants is able to detect PNP and produce riboflavin to boost electrical generation when co-cultured with Shewanella oneidensis. In the cathode, gene codes chromate (VI) reductase Yief was cloned into E.coli. The stability of MFCs has been improved and the electricity generated to correlated with the substrates. Moreover, based on this correlation, we’ve designed a software which is able to monitor the contaminants concentrations in MFCs.

 

 

 

  

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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