Team:LZU-China/interlab

From 2014.igem.org

(Difference between revisions)
Line 1: Line 1:
-
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "  
+
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
-
 
+
-
http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
+
<html xmlns=" http://www.w3.org/1999/xhtml">
<html xmlns=" http://www.w3.org/1999/xhtml">
<head>
<head>
Line 8: Line 6:
<style type="text/css">
<style type="text/css">
-
body,ul,li,ol{list-style:none;padding:0px;margin:0px;font-size:12px;font-family:微软
+
body,ul,li,ol{list-style:none;padding:0px;margin:0px;font-size:12px;font-family:微软雅黑;}
-
 
+
-
雅黑;}
+
img{border:0px;}
img{border:0px;}
.picList{width:100%;float:left;}
.picList{width:100%;float:left;}
-
.picList .col
+
.picList .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
-
 
+
-
{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0  
+
-
 
+
-
0 10px;}
+
.picList .col img,.col a{position:absolute;top:0px;left:0px;}
.picList .col img,.col a{position:absolute;top:0px;left:0px;}
.picList .col img{width:100%;}
.picList .col img{width:100%;}
-
.picList .col a{display:block;width:580;height:377px;top:357px;background:rgba
+
.picList .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
-
 
+
.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
-
(0,0,0,0.7);color:white;font-style:normal;line-
+
-
 
+
-
height:25px;padding:10px;opacity:0.8;text-decoration:none;}
+
-
.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-
+
-
 
+
-
size:14px;}
+
.picList2{width:100%;float:left;}
.picList2{width:100%;float:left;}
-
.picList2 .col
+
.picList2 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
-
 
+
-
{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0  
+
-
 
+
-
0 10px;}
+
.picList2 .col img,.col a{position:absolute;top:0px;left:0px;}
.picList2 .col img,.col a{position:absolute;top:0px;left:0px;}
.picList2 .col img{width:100%;}
.picList2 .col img{width:100%;}
-
.picList2 .col a{display:block;width:580;height:377px;top:357px;background:rgba
+
.picList2 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
-
 
+
.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
-
(0,0,0,0.7);color:white;font-style:normal;line-
+
-
 
+
-
height:25px;padding:10px;opacity:0.8;text-decoration:none;}
+
-
.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-
+
-
 
+
-
size:14px;}
+
.picList3{width:100%;float:left;}
.picList3{width:100%;float:left;}
-
.picList3 .col
+
.picList3 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
-
 
+
-
{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0  
+
-
 
+
-
0 10px;}
+
.picList3 .col img,.col a{position:absolute;top:0px;left:0px;}
.picList3 .col img,.col a{position:absolute;top:0px;left:0px;}
.picList3 .col img{width:100%;}
.picList3 .col img{width:100%;}
Line 67: Line 39:
text-decoration:none;
text-decoration:none;
}
}
-
.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-
+
.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
-
 
+
-
size:14px;}
+
.picList4{width:100%;float:left;}
.picList4{width:100%;float:left;}
-
.picList4 .col
+
.picList4 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
-
 
+
-
{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0  
+
-
 
+
-
0 10px;}
+
.picList4 .col img,.col a{position:absolute;top:0px;left:0px;}
.picList4 .col img,.col a{position:absolute;top:0px;left:0px;}
.picList4 .col img{width:100%;}
.picList4 .col img{width:100%;}
-
.picList4 .col a{display:block;width:580;height:377px;top:357px;background:rgba
+
.picList4 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
-
 
+
.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
-
(0,0,0,0.7);color:white;font-style:normal;line-
+
-
 
+
-
height:25px;padding:10px;opacity:0.8;text-decoration:none;}
+
-
.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-
+
-
 
+
-
size:14px;}
+
.picList5{width:100%;float:left;}
.picList5{width:100%;float:left;}
-
.picList5 .col
+
.picList5 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
-
 
+
-
{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0  
+
-
 
+
-
0 10px;}
+
.picList5 .col img,.col a{position:absolute;top:0px;left:0px;}
.picList5 .col img,.col a{position:absolute;top:0px;left:0px;}
.picList5 .col img{width:100%;}
.picList5 .col img{width:100%;}
-
.picList5 .col a{display:block;width:580;height:377px;top:357px;background:rgba
+
.picList5 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
-
 
+
.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
-
(0,0,0,0.7);color:white;font-style:normal;line-
+
-
 
+
-
height:25px;padding:10px;opacity:0.8;text-decoration:none;}
+
-
.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-
+
-
 
+
-
size:14px;}
+
.picList6{width:100%;float:left;}
.picList6{width:100%;float:left;}
-
.picList6 .col
+
.picList6 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
-
 
+
-
{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0  
+
-
 
+
-
0 10px;}
+
.picList6 .col img,.col a{position:absolute;top:0px;left:0px;}
.picList6 .col img,.col a{position:absolute;top:0px;left:0px;}
.picList6 .col img{width:100%;}
.picList6 .col img{width:100%;}
-
.picList6 .col a{display:block;width:580;height:377px;top:357px;background:rgba
+
.picList6 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
-
 
+
.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
-
(0,0,0,0.7);color:white;font-style:normal;line-
+
-
 
+
-
height:25px;padding:10px;opacity:0.8;text-decoration:none;}
+
-
.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-
+
-
 
+
-
size:14px;}
+
.picList7{width:100%;float:left;}
.picList7{width:100%;float:left;}
-
.picList7 .col
+
.picList7 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
-
 
+
-
{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0  
+
-
 
+
-
0 10px;}
+
.picList7 .col img,.col a{position:absolute;top:0px;left:0px;}
.picList7 .col img,.col a{position:absolute;top:0px;left:0px;}
.picList7 .col img{width:100%;}
.picList7 .col img{width:100%;}
-
.picList7 .col a{display:block;width:580;height:377px;top:357px;background:rgba
+
.picList7 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
-
 
+
.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
-
(0,0,0,0.7);color:white;font-style:normal;line-
+
-
 
+
-
height:25px;padding:10px;opacity:0.8;text-decoration:none;}
+
-
.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-
+
-
 
+
-
size:14px;}
+
.picList8{width:100%;float:left;}
.picList8{width:100%;float:left;}
-
.picList8 .col
+
.picList8 .col{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0 0 10px;}
-
 
+
-
{width:600px;height:397px;position:relative;overflow:hidden;float:left;margin:10px 0  
+
-
 
+
-
0 10px;}
+
.picList8 .col img,.col a{position:absolute;top:0px;left:0px;}
.picList8 .col img,.col a{position:absolute;top:0px;left:0px;}
.picList8 .col img{width:100%;}
.picList8 .col img{width:100%;}
-
.picList8 .col a{display:block;width:580;height:377px;top:357px;background:rgba
+
.picList8 .col a{display:block;width:580;height:377px;top:357px;background:rgba(0,0,0,0.7);color:white;font-style:normal;line-height:25px;padding:10px;opacity:0.8;text-decoration:none;}
-
 
+
.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-size:14px;}
-
(0,0,0,0.7);color:white;font-style:normal;line-
+
-
 
+
-
height:25px;padding:10px;opacity:0.8;text-decoration:none;}
+
-
.msg{float:left;width:900px;position:relative;left:50%;margin:30px 0 0 -450px;font-
+
-
 
+
-
size:14px;}
+
*{margin:0px;padding:0px;}
*{margin:0px;padding:0px;}
body{background:#b1b1b1;margin:0px;padding:0px;font-size:14px;color:#000;}
body{background:#b1b1b1;margin:0px;padding:0px;font-size:14px;color:#000;}
b#p1, b#p2, b#p3, b#p4, b#p5 {display:none;}
b#p1, b#p2, b#p3, b#p4, b#p5 {display:none;}
-
#accordian {padding:0; margin:20px auto; list-style:none; width:730px; height:300px;  
+
#accordian {padding:0; margin:20px auto; list-style:none; width:730px; height:300px; overflow:hidden; position:relative; border:1px solid #682;}
-
 
+
-
overflow:hidden; position:relative; border:1px solid #682;}
+
#accordian li {width:50px; height:300px; float:left;
#accordian li {width:50px; height:300px; float:left;
-moz-transition: 0.7s;
-moz-transition: 0.7s;
Line 170: Line 88:
}
}
#accordian div {width:530px; height:300px; position:relative;}
#accordian div {width:530px; height:300px; position:relative;}
-
#accordian div span {display:block; background:rgba(0,0,0,.3); width:460px;  
+
#accordian div span {display:block; background:rgba(0,0,0,.3); width:460px; height:30px; position:absolute; bottom:300px; right:0; padding:10px; color:#fff; font:normal 13px/30px 'georgia', serif; border-top:1px solid #000;
-
 
+
-
height:30px; position:absolute; bottom:300px; right:0; padding:10px; color:#fff;  
+
-
 
+
-
font:normal 13px/30px 'georgia', serif; border-top:1px solid #000;
+
}
}
#accordian div span a {color:#fff;}
#accordian div span a {color:#fff;}
-
#accordian li div > a {display:block; width:49px; height:300px; float:left;  
+
#accordian li div > a {display:block; width:49px; height:300px; float:left; position:relative; color:#fff; text-decoration:none;box-shadow:-10px 0 10px -10px rgba(0,0,0,0.6);}
-
 
+
#accordian li div > a b {display:block; width:280px; padding-left:20px; height:50px; position:absolute; left:50px; bottom:0; font:bold 24px/50px 'georgia', serif; text-shadow:0 0 2px rgba(0, 0, 0, 0.6);
-
position:relative; color:#fff; text-decoration:none;box-shadow:-10px 0 10px -10px  
+
-
 
+
-
rgba(0,0,0,0.6);}
+
-
#accordian li div > a b {display:block; width:280px; padding-left:20px; height:50px;  
+
-
 
+
-
position:absolute; left:50px; bottom:0; font:bold 24px/50px 'georgia', serif; text-
+
-
 
+
-
shadow:0 0 2px rgba(0, 0, 0, 0.6);
+
transform: rotate(-90deg);
transform: rotate(-90deg);
transform-origin:bottom left;
transform-origin:bottom left;
Line 229: Line 135:
-
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter,  
+
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {display: none;} /*-- hides default wiki settings --*/
-
 
+
-
.visualClear {display: none;} /*-- hides default wiki settings --*/
+
.firstHeading {
.firstHeading {
Line 255: Line 159:
}
}
-
#top-section-bar { /*-- styling full width bar which hides behind default menu bar  
+
#top-section-bar { /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/
-
 
+
-
(edit, page, history, etc.) --*/
+
background-color: #383838;
background-color: #383838;
height: 14px;
height: 14px;
Line 267: Line 169:
}
}
-
#menubar a:link, #menubar a:active, #menubar a:visited, #menubar a:hover,  
+
#menubar a:link, #menubar a:active, #menubar a:visited, #menubar a:hover, #menubar:hover { /*-- styling for default menu bar links (edit, page, history, etc.) --*/
-
 
+
-
#menubar:hover { /*-- styling for default menu bar links (edit, page, history, etc.)  
+
-
 
+
-
--*/
+
color: #727272;  
color: #727272;  
text-decoration: none;  
text-decoration: none;  
Line 444: Line 342:
@charset "utf-8";
@charset "utf-8";
-
body,h1,h2,h3,h4,h5,h6,hr,p,blockquote,dl,dt,dd,ul,ol,li,pre,form,fieldset,legend,bu
+
body,h1,h2,h3,h4,h5,h6,hr,p,blockquote,dl,dt,dd,ul,ol,li,pre,form,fieldset,legend,button,input,textarea,th,td,figure{
-
 
+
-
tton,input,textarea,th,td,figure{
+
margin:0;
margin:0;
padding:0;
padding:0;
Line 455: Line 351:
table{table-layout:fixed;border-collapse:collapse}
table{table-layout:fixed;border-collapse:collapse}
section,article,aside,header,footer,nav,dialog,figure{display:block}
section,article,aside,header,footer,nav,dialog,figure{display:block}
-
.clearfix:after{content:"\20";display:block;height:0;line-height:0;font-
+
.clearfix:after{content:"\20";display:block;height:0;line-height:0;font-size:0;clear:both}
-
 
+
body,button,input,select,textarea,td,th{color:#333;font:12px/1.5 tahoma,arial,\5b8b\4f53}
-
size:0;clear:both}
+
-
body,button,input,select,textarea,td,th{color:#333;font:12px/1.5  
+
-
 
+
-
tahoma,arial,\5b8b\4f53}
+
h1,h2,h3,h4,h5,h6{font-size:100%}
h1,h2,h3,h4,h5,h6{font-size:100%}
address,cite,dfn,em,var{font-style:normal}
address,cite,dfn,em,var{font-style:normal}
Line 477: Line 369:
}
}
input:focus{outline:none}
input:focus{outline:none}
-
.hd-main{height:60px;background:#fff;z-index:15;border-bottom:#b3b3b3 1px solid;box
+
.hd-main{height:60px;background:#fff;z-index:15;border-bottom:#b3b3b3 1px solid;box-shadow:0 0 5px #333}
-
 
+
.f-icon{background-image:url('http://www.codefans.net/jscss/demoimg/201403//frame-icon.png');background-repeat:no-repeat}
-
-shadow:0 0 5px #333}
+
.hd-main{padding:0 5px;height:49px;min-width:800px;background:#252525;border-bottom:0}
-
.f-icon{background-image:url('http://www.codefans.net/jscss/demoimg/201403//frame-
+
-
 
+
-
icon.png');background-repeat:no-repeat}
+
-
.hd-main{padding:0 5px;height:49px;min-width:800px;background:#252525;border-
+
-
 
+
-
bottom:0}
+
.hd-main .has-pulldown{cursor:pointer;position:relative;*z-index:10000}
.hd-main .has-pulldown{cursor:pointer;position:relative;*z-index:10000}
.hd-main .pulldown{position:absolute;cursor:default;display:none;top:30px;left:0}
.hd-main .pulldown{position:absolute;cursor:default;display:none;top:30px;left:0}
-
.hd-main .pulldown .arrow{*margin-bottom:-1px;_margin-
+
.hd-main .pulldown .arrow{*margin-bottom:-1px;_margin-bottom:0;height:0;width:0;font-size:0;line-height:0;border-width:7px;border-style:solid;border-color:transparent transparent rgba(255, 255, 255,0.8) transparent;_filter:chroma(color=tomato);_border-color:tomato tomato rgba(255, 255, 255,0.8) tomato;position:relative;display:block;left:20px;z-index:7}
-
 
+
.hd-main .pulldown .content{background:rgba(255, 255, 255,0.8);color:#333;text-align:left;border-radius:3px;border:rgb(175,175,175) 1px solid;border-width:0 1px 1px 1px;box-shadow:0 2px 4px rgba(0,0,0,.2);position:relative;z-index:6}
-
bottom:0;height:0;width:0;font-size:0;line-height:0;border-width:7px;border-
+
.hd-main .pulldown-canvas{position:absolute;top:0;left:0;width:100%;height:100%;opacity:0;filter:alpha(opacity=0)}
-
 
+
.hd-main .logo{background-image:url('http://2014.igem.org/wiki/images/7/72/2014logomin.png');background-repeat:no-repeat;display:block;height:36px;width:175px;float:left;margin-left:10px;background-position:0 0;margin-top:5px;display:inline}
-
style:solid;border-color:transparent transparent rgba(255, 255, 255,0.8)  
+
-
 
+
-
transparent;_filter:chroma(color=tomato);_border-color:tomato tomato rgba(255, 255,  
+
-
 
+
-
255,0.8) tomato;position:relative;display:block;left:20px;z-index:7}
+
-
.hd-main .pulldown .content{background:rgba(255, 255, 255,0.8);color:#333;text-
+
-
 
+
-
align:left;border-radius:3px;border:rgb(175,175,175) 1px solid;border-width:0 1px  
+
-
 
+
-
1px 1px;box-shadow:0 2px 4px rgba(0,0,0,.2);position:relative;z-index:6}
+
-
.hd-main .pulldown-canvas
+
-
 
+
-
{position:absolute;top:0;left:0;width:100%;height:100%;opacity:0;filter:alpha
+
-
 
+
-
(opacity=0)}
+
-
.hd-main .logo{background-image:url
+
-
 
+
-
('http://2014.igem.org/wiki/images/7/72/2014logomin.png');background-repeat:no-
+
-
 
+
-
repeat;display:block;height:36px;width:175px;float:left;margin-left:10px;background
+
-
 
+
-
-position:0 0;margin-top:5px;display:inline}
+
.hd-main .navs{padding:0 0 3px 25px;height:45px;float:left}
.hd-main .navs{padding:0 0 3px 25px;height:45px;float:left}
-
.hd-main .navs a,.hd-main .info a,.hd-main .navs a:visited,.hd-main .info  
+
.hd-main .navs a,.hd-main .info a,.hd-main .navs a:visited,.hd-main .info a:visited,.hd-main .navs a:active,.hd-main .info a:active{color:#d8d8d8}
-
 
+
.hd-main .navs a:hover,.hd-main .navs a:active{text-decoration:none;background-color:#0f0f0f}
-
a:visited,.hd-main .navs a:active,.hd-main .info a:active{color:#d8d8d8}
+
.hd-main .navs .def-nav,.hd-main .navs .pulldown-nav,.hd-main .navs .separate{display:block;float:left;height:48px;font:18px/30px "Microsoft YaHei","Microsoft JhengHei";color:#d8d8d8;text-align:center;width:125px;line-height:48px}
-
.hd-main .navs a:hover,.hd-main .navs a:active{text-decoration:none;background-
+
-
 
+
-
color:#0f0f0f}
+
-
.hd-main .navs .def-nav,.hd-main .navs .pulldown-nav,.hd-main .navs .separate
+
-
 
+
-
{display:block;float:left;height:48px;font:18px/30px "Microsoft YaHei","Microsoft  
+
-
 
+
-
JhengHei";color:#d8d8d8;text-align:center;width:125px;line-height:48px}
+
.hd-main .navs .def-nav .app-url{left:185px;width:425px;top:48px}
.hd-main .navs .def-nav .app-url{left:185px;width:425px;top:48px}
.hd-main .navs .def-nav .pulldown-canvas-url{width:575px;height:109px}
.hd-main .navs .def-nav .pulldown-canvas-url{width:575px;height:109px}
.hd-main .navs .def-nav .app-url .arrow{left:140px}
.hd-main .navs .def-nav .app-url .arrow{left:140px}
.hd-main .navs .app-url .content{padding:17px 0 0 22px;height:91px}
.hd-main .navs .app-url .content{padding:17px 0 0 22px;height:91px}
-
.hd-main .navs .app-url .content a,.hd-main .navs .app-url .content a:hover
+
.hd-main .navs .app-url .content a,.hd-main .navs .app-url .content a:hover{color:#333}
-
 
+
.hd-main .navs .app-url .li{cursor:pointer;padding-top:8px;width:66px;height:66px;border-radius:3px;display:block;float:left;text-align:center}
-
{color:#333}
+
-
.hd-main .navs .app-url .li{cursor:pointer;padding-
+
-
 
+
-
top:8px;width:66px;height:66px;border-radius:3px;display:block;float:left;text-
+
-
 
+
-
align:center}
+
.hd-main .navs .app-url .li:hover{background-color:#e0e1e5}
.hd-main .navs .app-url .li:hover{background-color:#e0e1e5}
-
.hd-main .navs .app-url .li .icon{margin:0  
+
.hd-main .navs .app-url .li .icon{margin:0 auto;width:50px;height:50px;display:block;background:url('http://2014.igem.org/wiki/images/9/94/Icons50X50nb.png') no-repeat}
-
 
+
.hd-main .navs .app-url .li:hover .icon{background-image:url('http://2014.igem.org/wiki/images/9/94/Icons50X50nb.png')}
-
auto;width:50px;height:50px;display:block;background:url
+
-
 
+
-
('http://2014.igem.org/wiki/images/9/94/Icons50X50nb.png') no-repeat}
+
-
.hd-main .navs .app-url .li:hover .icon{background-image:url
+
-
 
+
-
('http://2014.igem.org/wiki/images/9/94/Icons50X50nb.png')}
+
.hd-main .navs .app-url .tongxunlu .icon{background-position:-50px 0}
.hd-main .navs .app-url .tongxunlu .icon{background-position:-50px 0}
.hd-main .navs .app-url .xiangce .icon{background-position:-100px 0}
.hd-main .navs .app-url .xiangce .icon{background-position:-100px 0}
Line 553: Line 397:
.hd-main .navs .app-url .zhaohui .icon{background-position:-250px 0}
.hd-main .navs .app-url .zhaohui .icon{background-position:-250px 0}
.hd-main .navs .app-url .youxi .icon{background-position:-300px 0}
.hd-main .navs .app-url .youxi .icon{background-position:-300px 0}
-
.hd-main .navs .app-url .li .text{height:30px;display:block;font:10px/30px  
+
.hd-main .navs .app-url .li .text{height:30px;display:block;font:10px/30px "Microsoft YaHei","Microsoft JhengHei"}
-
 
+
-
"Microsoft YaHei","Microsoft JhengHei"}
+
.hd-main .navs .separate{margin:0;height:48px;width:2px;background:#2A2B2D}
.hd-main .navs .separate{margin:0;height:48px;width:2px;background:#2A2B2D}
.hd-main .navs .pulldown-nav{position:relative}
.hd-main .navs .pulldown-nav{position:relative}
Line 568: Line 410:
background-position:-89px -45px
background-position:-89px -45px
}
}
-
.hd-main .navs .pulldown-nav:hover .f-icon,.hd-main .navs .pulldown-nav:active .f-
+
.hd-main .navs .pulldown-nav:hover .f-icon,.hd-main .navs .pulldown-nav:active .f-icon{position:absolute;display:block;right:11px;top:18px;height:12px;width:12px;font-size:0;background-position:-89px -55px}
-
 
+
.hd-main .navs .current,.hd-main .navs .current .pulldown-nav{font-weight:bold;color:#FFF;display:inline-block;width:125px;height:49px;background-color:#0f0f0f;text-align:center;line-height:48px}
-
icon
+
-
 
+
-
{position:absolute;display:block;right:11px;top:18px;height:12px;width:12px;font-
+
-
 
+
-
size:0;background-position:-89px -55px}
+
-
.hd-main .navs .current,.hd-main .navs .current .pulldown-nav{font-
+
-
 
+
-
weight:bold;color:#FFF;display:inline-block;width:125px;height:49px;background-
+
-
 
+
-
color:#0f0f0f;text-align:center;line-height:48px}
+
.hd-main .navs .current:hover{color:#ECECEC}
.hd-main .navs .current:hover{color:#ECECEC}
.hd-main .navs .current .hover{background-color:#dedede;color:#333;}
.hd-main .navs .current .hover{background-color:#dedede;color:#333;}
-
.hd-main .info,.hd-main .info ul{height:36px;float:right;text-align:right;margin-
+
.hd-main .info,.hd-main .info ul{height:36px;float:right;text-align:right;margin-top:3px;white-space:nowrap}
-
 
+
-
top:3px;white-space:nowrap}
+
.hd-main .info a{color:#d8d8d8}
.hd-main .info a{color:#d8d8d8}
.hd-main .info a:hover{color:#FFF;text-decoration:underline}
.hd-main .info a:hover{color:#FFF;text-decoration:underline}
-
.hd-main .info .info-i{padding:0 10px;margin:10px 0;display:inline-
+
.hd-main .info .info-i{padding:0 10px;margin:10px 0;display:inline-block;*display:inline;*zoom:1;height:16px;border-right:#393939 1px solid;color:#d8d8d8;font:12px/16px "SimSun"}
-
 
+
.hd-main{padding:0 5px;height:49px;min-width:800px;background:#252525;border-bottom:0}
-
block;*display:inline;*zoom:1;height:16px;border-right:#393939 1px  
+
-
 
+
-
solid;color:#d8d8d8;font:12px/16px "SimSun"}
+
-
.hd-main{padding:0 5px;height:49px;min-width:800px;background:#252525;border-
+
-
 
+
-
bottom:0}
+
.hd-main .info .has-pulldown{padding-right:28px}
.hd-main .info .has-pulldown{padding-right:28px}
-
.hd-main .info .has-pulldown .pull-arrow
+
.hd-main .info .has-pulldown .pull-arrow{position:absolute;display:block;right:10px;top:1px;height:12px;width:12px;font-size:0;background-position:-89px -45px}
-
 
+
-
{position:absolute;display:block;right:10px;top:1px;height:12px;width:12px;font-
+
-
 
+
-
size:0;background-position:-89px -45px}
+
.hd-main .info .no-separate{border:none}
.hd-main .info .no-separate{border:none}
.hd-main .info .user-name{position:relative;padding-left:24px}
.hd-main .info .user-name{position:relative;padding-left:24px}
-
.hd-main .info .user-name .name{display:inline-block;display:inline\0;white-
+
.hd-main .info .user-name .name{display:inline-block;display:inline\0;white-space:nowrap;max-width:75px;overflow:hidden;text-overflow:ellipsis;vertical-align:bottom}
-
 
+
.hd-main .info .user-name .identity{position:absolute;top:0;left:0;height:16px;width:16px;background-position:-89px -88px}
-
space:nowrap;max-width:75px;overflow:hidden;text-overflow:ellipsis;vertical-
+
-
 
+
-
align:bottom}
+
-
.hd-main .info .user-name .identity
+
-
 
+
-
{position:absolute;top:0;left:0;height:16px;width:16px;background-position:-89px -
+
-
 
+
-
88px}
+
.hd-main .info .user-name .is-vip{background-position:-89px -68px}
.hd-main .info .user-name .is-vip{background-position:-89px -68px}
-
.hd-main .info .user-info,.hd-main .info .more-info{top:9px;left:auto;right:-
+
.hd-main .info .user-info,.hd-main .info .more-info{top:9px;left:auto;right:-24px;width:170px} /*User栏目宽度*/
-
 
+
.hd-main .info .notice-nu{display:inline-block;padding-left:4px;background-position:-86px -109px;height:14px;-webkit-text-size-adjust:none;font-size:10px;font-style:normal}
-
24px;width:170px} /*User栏目宽度*/
+
.hd-main .info .notice-nu .f-icon{display:inline-block;padding-right:4px;background-position:right -109px;color:#FFF}  /*此处原为#FFF*/
-
.hd-main .info .notice-nu{display:inline-block;padding-left:4px;background-
+
-
 
+
-
position:-86px -109px;height:14px;-webkit-text-size-adjust:none;font-size:10px;font
+
-
 
+
-
-style:normal}
+
-
.hd-main .info .notice-nu .f-icon{display:inline-block;padding-right:4px;background
+
-
 
+
-
-position:right -109px;color:#FFF}  /*此处原为#FFF*/
+
.hd-main .info .user-info .arrow{left:70px;*left:-32px}
.hd-main .info .user-info .arrow{left:70px;*left:-32px}
-
.hd-main .info .user-info .content,.hd-main .info .more-info .content{padding:10px  
+
.hd-main .info .user-info .content,.hd-main .info .more-info .content{padding:10px 0;height:180px}
-
 
+
-
0;height:180px}
+
.hd-main .info .more{padding:0;display:inline;line-height:inherit;background:none}
.hd-main .info .more{padding:0;display:inline;line-height:inherit;background:none}
.hd-main .info .more-info .content{height:150px}
.hd-main .info .more-info .content{height:150px}
-
.hd-main .info .user-info .content a,.hd-main .info .more-info .content a
+
.hd-main .info .user-info .content a,.hd-main .info .more-info .content a{display:block;padding-left:33px;height:30px;line-height:30px;color:#888}
-
 
+
.hd-main .info .user-info .content a:hover,.hd-main .info .more-info .content a:hover{color:#333;text-decoration:none;background:#e0e1e5}
-
{display:block;padding-left:33px;height:30px;line-height:30px;color:#888}
+
.hd-main .info .user-info .li,.hd-main .info .more-info .li{display:block;padding:2px 0;height:30px}
-
.hd-main .info .user-info .content a:hover,.hd-main .info .more-info .content  
+
.hd-main .info .user-info .separate-li,.hd-main .info .more-info .separate-li{display:block;margin:3px 11px;border-top:#EBEBEC 1px solid;border-bottom:#FFF 1px solid}
-
 
+
-
a:hover{color:#333;text-decoration:none;background:#e0e1e5}
+
-
.hd-main .info .user-info .li,.hd-main .info .more-info .li
+
-
 
+
-
{display:block;padding:2px 0;height:30px}
+
-
.hd-main .info .user-info .separate-li,.hd-main .info .more-info .separate-li
+
-
 
+
-
{display:block;margin:3px 11px;border-top:#EBEBEC 1px solid;border-bottom:#FFF 1px  
+
-
 
+
-
solid}
+
.hd-main .info .more-info{right:0}
.hd-main .info .more-info{right:0}
.hd-main .info .more-info .arrow{left:94px;*left:-8px}
.hd-main .info .more-info .arrow{left:94px;*left:-8px}
Line 665: Line 457:
background-color: #FFF;
background-color: #FFF;
}
}
-
#menu {right:0; top:0; width:100%; position:fixed; padding:10px; text-align:center;  
+
#menu {right:0; top:0; width:100%; position:fixed; padding:10px; text-align:center; font-weight:bold; background:rgba(0,83,163,0);}
-
 
+
-
font-weight:bold; background:rgba(0,83,163,0);}
+
* html #menu {position:absolute; right:16px; top:0;}/*only for ie*/
* html #menu {position:absolute; right:16px; top:0;}/*only for ie*/
html {overflow:auto !important; overflow:hidden;}
html {overflow:auto !important; overflow:hidden;}
Line 836: Line 626:
   <table width="100%" border="0" >
   <table width="100%" border="0" >
     <tr>
     <tr>
-
       <td width="1201" height="265" rowspan="2"><p><img  
+
       <td width="1201" height="265" rowspan="2"><p><img src="http://2014.igem.org/wiki/images/9/91/7interlab.top.jpg" width="1366" height="270" /></p></td>
-
 
+
-
src="http://2014.igem.org/wiki/images/9/91/7interlab.top.jpg" width="1366"  
+
-
 
+
-
height="270" /></p></td>
+
       <td width="100%" height="199" bgcolor="#187ADB">&nbsp;</td>
       <td width="100%" height="199" bgcolor="#187ADB">&nbsp;</td>
     </tr>
     </tr>
Line 855: Line 641:
         <div>Our Interlab</div>
         <div>Our Interlab</div>
         <p>&nbsp;</p>
         <p>&nbsp;</p>
-
         <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<span lang="EN-US"  
+
         <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<span lang="EN-US" xml:lang="EN-US">In our interlab work we test the three required parts. They are:</span></p>
-
 
+
-
xml:lang="EN-US">In our interlab work we test the three required parts. They  
+
-
 
+
-
are:</span></p>
+
         <p>&nbsp;</p>
         <p>&nbsp;</p>
         <ul>
         <ul>
           <li>
           <li>
             <!--[if !supportLists]-->
             <!--[if !supportLists]-->
-
             <strong><span lang="EN-US" xml:lang="EN-
+
             <strong><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.</span></strong>
-
 
+
-
US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&n
+
-
 
+
-
bsp;1.</span></strong>
+
             <!--[endif]-->
             <!--[endif]-->
-
           <strong><span lang="EN-US" xml:lang="EN-US">BBa_I20260 (J23101-B0032-
+
           <strong><span lang="EN-US" xml:lang="EN-US">BBa_I20260 (J23101-B0032-E0040-B0015) Plate 4,Well 18A</span></strong>        </li>
-
 
+
-
E0040-B0015) Plate 4,Well 18A</span></strong>        </li>
+
         </ul>
         </ul>
         <ul>
         <ul>
           <li>
           <li>
             <!--[if !supportLists]-->
             <!--[if !supportLists]-->
-
             <strong><span lang="EN-US" xml:lang="EN-
+
             <strong><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.</span></strong>
-
 
+
-
US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&n
+
-
 
+
-
bsp;2.</span></strong>
+
             <!--[endif]-->
             <!--[endif]-->
-
           <strong><span lang="EN-US" xml:lang="EN-US">BBa_J23101 + BBa_E0240 (B0032
+
           <strong><span lang="EN-US" xml:lang="EN-US">BBa_J23101 + BBa_E0240 (B0032-E0040-B0015) Plate 1,Well 20K and Plate 2,Well 24B</span></strong><strong><span lang="EN-US" xml:lang="EN-US">&nbsp;</span></strong></li>
-
 
+
-
-E0040-B0015) Plate 1,Well 20K and Plate 2,Well 24B</span></strong><strong><span  
+
-
 
+
-
lang="EN-US" xml:lang="EN-US">&nbsp;</span></strong></li>
+
         </ul>
         </ul>
-
<p><strong><span lang="EN-US" xml:lang="EN-
+
<p><strong><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.BBa_J23115 + BBa_E0240 (B0032-E0040-B0015) Plate 1,Well 22I and Plate 2,Well 24B</span></strong></p>
-
 
+
-
US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.BBa_J23115 +  
+
-
 
+
-
BBa_E0240 (B0032-E0040-B0015) Plate 1,Well 22I and Plate 2,Well  
+
-
 
+
-
24B</span></strong></p>
+
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
Line 905: Line 667:
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
-
         <div><img src="http://2014.igem.org/wiki/images/f/f7/Interlab_image002.jpg" alt="" width="531" height="240"  
+
         <div><img src="http://2014.igem.org/wiki/images/f/f7/Interlab_image002.jpg" alt="" width="531" height="240" v:shapes="_x0000_i1025" /></div>
-
 
+
         <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Figure-1 The gel picture of each device</p>
-
v:shapes="_x0000_i1025" /></div>
+
         <div><img src="http://2014.igem.org/File:LZUimage003.png" alt="" width="408" height="369" v:shapes="_x0000_i1025" /></div>
-
          
+
         <p><span lang="EN-US" xml:lang="EN-US"> </span><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Table-1 Each lane&rsquo;s properties</span></p>
-
 
+
-
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nb
+
-
 
+
-
sp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nb
+
-
 
+
-
sp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nb
+
-
 
+
-
sp;Figure-1 The gel picture of each device</p>
+
-
         <div><img src="http://2014.igem.org/File:LZUimage003.png" alt=""  
+
-
 
+
-
width="408" height="369" v:shapes="_x0000_i1025" /></div>
+
-
         <p><span lang="EN-US" xml:lang="EN-US"> </span><span lang="EN-US"  
+
-
 
+
-
xml:lang="EN-
+
-
 
+
-
US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&n
+
-
 
+
-
bsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&n
+
-
 
+
-
bsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&n
+
-
 
+
-
bsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&n
+
-
 
+
-
bsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Table-1 Each  
+
-
 
+
-
lane&rsquo;s properties</span></p>
+
         <p>&nbsp;</p>
         <p>&nbsp;</p>
         <p>&nbsp;</p>
         <p>&nbsp;</p>
Line 940: Line 676:
         <p>&nbsp;</p>
         <p>&nbsp;</p>
<p><span lang="EN-US" xml:lang="EN-US">Equipment and chemicals</span></p>
<p><span lang="EN-US" xml:lang="EN-US">Equipment and chemicals</span></p>
-
         <p><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp; 20</span><span lang="EN
+
         <p><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp; 20</span><span lang="EN-US" xml:lang="EN-US">&mu;</span><span lang="EN-US" xml:lang="EN-US">L eppendorf tubes x9</span></p>
-
 
+
-
-US" xml:lang="EN-US">&mu;</span><span lang="EN-US" xml:lang="EN-US">L eppendorf  
+
-
 
+
-
tubes x9</span></p>
+
         <p><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp; ddH2O</span></p>
         <p><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp; ddH2O</span></p>
-
         <p><em><span lang="EN-US" xml:lang="EN-US">&nbsp; </span></em><span  
+
         <p><em><span lang="EN-US" xml:lang="EN-US">&nbsp; </span></em><span lang="EN-US" xml:lang="EN-US">&nbsp;</span><span lang="EN-US" xml:lang="EN-US">Competent </span><em><span lang="EN-US" xml:lang="EN-US">E.Coli </span></em></p>
-
 
+
         <p><em><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp; </span></em><span lang="EN-US" xml:lang="EN-US">The devices</span></p>
-
lang="EN-US" xml:lang="EN-US">&nbsp;</span><span lang="EN-US" xml:lang="EN-
+
         <p><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp; Fluorescence microscope.</span></p>
-
 
+
-
US">Competent </span><em><span lang="EN-US" xml:lang="EN-US">E.Coli </span></em></p>
+
-
         <p><em><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp; </span></em><span  
+
-
 
+
-
lang="EN-US" xml:lang="EN-US">The devices</span></p>
+
-
         <p><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp; Fluorescence  
+
-
 
+
-
microscope.</span></p>
+
         <p><span lang="EN-US" xml:lang="EN-US">Methods:</span></p>
         <p><span lang="EN-US" xml:lang="EN-US">Methods:</span></p>
-
         <p><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp; Each device was  
+
         <p><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp; Each device was transformed in competent <em>E.Coli </em>and shaken cultured at 37℃ for 18h. Then we detected the number of green light spot by software. The final result is the average light spot , which are more bright than G value 192 for 3 random sites of each site by the </span><span lang="EN-US" xml:lang="EN-US">algorithm which we used to developed.</span></p>
-
 
+
-
transformed in competent <em>E.Coli </em>and shaken cultured at 37℃ for 18h. Then  
+
-
 
+
-
we detected the number of green light spot by software. The final result is the  
+
-
 
+
-
average light spot , which are more bright than G value 192 for 3 random sites of  
+
-
 
+
-
each site by the </span><span lang="EN-US" xml:lang="EN-US">algorithm which we used  
+
-
 
+
-
to developed.</span></p>
+
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
Line 975: Line 689:
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
-
<div><img src="http://2014.igem.org/wiki/images/a/a9/LZUimage005.png" width="520"  
+
<div><img src="http://2014.igem.org/wiki/images/a/a9/LZUimage005.png" width="520" height="429" /></div>
-
 
+
-
height="429" /></div>
+
<p>&nbsp;</p>
<p>&nbsp;</p>
-
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Picture  
+
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Picture 1-3 are the device 1's results, Picture 4-6 are the device 2's and the Picture 7-9 are the device 3's. Each picture is 400x and we calculated the light spot. The result are listed here:</p>
-
 
+
-
1-3 are the device 1's results, Picture 4-6 are the device 2's and the Picture 7-9  
+
-
 
+
-
are the device 3's. Each picture is 400x and we calculated the light spot. The  
+
-
 
+
-
result are listed here:</p>
+
<div><img src="http://2014.igem.org/File:LZUIMAGE4.png" alt="" width="653" height="282" /></div>
<div><img src="http://2014.igem.org/File:LZUIMAGE4.png" alt="" width="653" height="282" /></div>
<p>&nbsp;</p>
<p>&nbsp;</p>
-
       <div><img src="http://2014.igem.org/wiki/images/3/3e/Image006.png" alt=""  
+
       <div><img src="http://2014.igem.org/wiki/images/3/3e/Image006.png" alt="" width="569" height="279" /></div>
-
 
+
       <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Figure-3 The &quot;light&quot; spot number of each devices</p>
-
width="569" height="279" /></div>
+
-
        
+
-
 
+
-
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Figure-3 The &quot;light&quot; spot  
+
-
 
+
-
number of each devices</p>
+
       <p>&nbsp;</p>
       <p>&nbsp;</p>
-
       <div><img src="http://2014.igem.org/wiki/images/1/16/LZUIMAGE7.png" alt=""  
+
       <div><img src="http://2014.igem.org/wiki/images/1/16/LZUIMAGE7.png" alt="" width="697" height="123" /></div>
-
 
+
       <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;     Table-3  The mean and standard deviation of each device <br />
-
width="697" height="123" /></div>
+
-
        
+
-
 
+
-
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Table-3  The mean and standard  
+
-
 
+
-
deviation of each device <br />
+
       </p>
       </p>
       <p>&nbsp;</p>
       <p>&nbsp;</p>
-
        
+
       <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Besides, we used the cytoflower to detect the  number of cells with green fluorescence. The result is here:
-
 
+
         (Tube 001 is the control. Tube 002 and 003 are  Device 2 . Tube 004 is Device 1 and Tube 005 is Device 3. )</p>
-
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nb
+
       <div><img src="http://2014.igem.org/wiki/images/8/85/Lzuinter_(2).png" alt="" width="401" height="514" /></div>
-
 
+
-
sp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Besides, we used the cytoflower to detect  
+
-
 
+
-
the  number of cells with green fluorescence. The result is here:
+
-
         (Tube 001 is the control. Tube 002 and 003 are  Device 2 . Tube 004 is  
+
-
 
+
-
Device 1 and Tube 005 is Device 3. )</p>
+
-
       <div><img src="http://2014.igem.org/wiki/images/8/85/Lzuinter_%282%29.png"  
+
-
 
+
-
alt="" width="401" height="514" /></div>
+
       <p>&nbsp;</p>
       <p>&nbsp;</p>
-
       <div><img src="http://2014.igem.org/wiki/images/5/56/Lzuinter_%283%29.png"  
+
       <div><img src="http://2014.igem.org/wiki/images/5/56/Lzuinter_(3).png" alt="" width="436" height="515" /></div>
-
 
+
-
alt="" width="436" height="515" /></div>
+
       <p>&nbsp;</p>
       <p>&nbsp;</p>
-
       <div><img src="http://2014.igem.org/wiki/images/8/89/Lzuinter_%281%29.png"  
+
       <div><img src="http://2014.igem.org/wiki/images/8/89/Lzuinter_(1).png" alt="" width="465" height="272" /></div>
-
 
+
-
alt="" width="465" height="272" /></div>
+
       <p>&nbsp;</p>
       <p>&nbsp;</p>
       <p>&nbsp;</p>
       <p>&nbsp;</p>
Line 1,037: Line 717:
<p> Qi Wu, Hongxia Zhao and Ruixue Zhao.</p>
<p> Qi Wu, Hongxia Zhao and Ruixue Zhao.</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
-
       <p>2.What other people should be credited for these measurements? (i.e., who  
+
       <p>2.What other people should be credited for these measurements? (i.e., who would be an author on any resulting publication. For example, your faculty advisor may have helped design the protocols that you ran.)</p>
-
 
+
-
would be an author on any resulting publication. For example, your faculty advisor  
+
-
 
+
-
may have helped design the protocols that you ran.)</p>
+
       <p> Xiangkai Li will be the &quot;corresponding author&quot;.</p>
       <p> Xiangkai Li will be the &quot;corresponding author&quot;.</p>
       <p>&nbsp;</p>
       <p>&nbsp;</p>
-
       <p>3.On what dates were the protocols run and the measurements taken? (this  
+
       <p>3.On what dates were the protocols run and the measurements taken? (this will often be a range of dates; make sure you say which data was taken at what times.)</p>
-
 
+
       <p> We ran the protocol on2014.10.4 because of the mistake and we took the simple fluorescence data on 2014.10.16. The data made by flow cytometer was got on 2014.10.17.</p>
-
will often be a range of dates; make sure you say which data was taken at what  
+
-
 
+
-
times.)</p>
+
-
       <p> We ran the protocol on2014.10.4 because of the mistake and we took the  
+
-
 
+
-
simple fluorescence data on 2014.10.16. The data made by flow cytometer was got on  
+
-
 
+
-
2014.10.17.</p>
+
       <p>&nbsp;</p>
       <p>&nbsp;</p>
-
       <p>4.Do all persons involved consent to the inclusion of this data in  
+
       <p>4.Do all persons involved consent to the inclusion of this data in publications derived from the iGEM interlab study?</p>
-
 
+
-
publications derived from the iGEM interlab study?</p>
+
       <p> Yes.<br />
       <p> Yes.<br />
       </p>
       </p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
-
<div><strong><span lang="EN-US" xml:lang="EN-US">S<em>ection II:  
+
<div><strong><span lang="EN-US" xml:lang="EN-US">S<em>ection II: Protocol</em></span></strong></div>
-
 
+
-
Protocol</em></span></strong></div>
+
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
Line 1,073: Line 737:
<p>o  What is the model and manufacturer?</p>
<p>o  What is the model and manufacturer?</p>
<p> Fluorescence microscope is Nikon OPTIPHOT-2 .</p>
<p> Fluorescence microscope is Nikon OPTIPHOT-2 .</p>
-
<p>o  How is it configured for your measurements? (e.g., light filters,  
+
<p>o  How is it configured for your measurements? (e.g., light filters, illumination, amplification)</p>
-
 
+
-
illumination, amplification)</p>
+
<p> The microscope's exciting light was set as 492nm. </p>
<p> The microscope's exciting light was set as 492nm. </p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>3.What protocol did you use to take measurements?</p>
<p>3.What protocol did you use to take measurements?</p>
-
<p> The transformed E coli. Was shaken cultured about 17h and we took photo by the  
+
<p> The transformed E coli. Was shaken cultured about 17h and we took photo by the fluorescence microscope.Then we analyzed the number of light spots by software.</p>
-
 
+
-
fluorescence microscope.Then we analyzed the number of light spots by software.</p>
+
<p>&nbsp;</p>
<p>&nbsp;</p>
-
<p>4.What method is used to determine whether to include or exclude each sample from  
+
<p>4.What method is used to determine whether to include or exclude each sample from the data set?</p>
-
 
+
<p> After the scoping we did electrophoresis for each tube to exam if the bacteria had been transformed.</p>
-
the data set?</p>
+
-
<p> After the scoping we did electrophoresis for each tube to exam if the bacteria  
+
-
 
+
-
had been transformed.</p>
+
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>5.What exactly were the controls that you used?</p>
<p>5.What exactly were the controls that you used?</p>
<p> DH5 Alpha without transforming which had been shaken cultured for 17h.</p>
<p> DH5 Alpha without transforming which had been shaken cultured for 17h.</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
-
<p>6.What quantities were measured? (e.g., red fluorescence, green fluorescence,  
+
<p>6.What quantities were measured? (e.g., red fluorescence, green fluorescence, optical density)</p>
-
 
+
-
optical density)</p>
+
<p> Green fluorescence. For the three reporters are all GFP.</p>
<p> Green fluorescence. For the three reporters are all GFP.</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>7.How much time did it take to acquire each set of measurements?</p>
<p>7.How much time did it take to acquire each set of measurements?</p>
-
<p> The data was got on the same day. While different device was transformed  
+
<p> The data was got on the same day. While different device was transformed successfully on different date. Device 1 took more time, about 7 days and was got on October 13th. Device 2 and 3 were transformed successfully on 10.14, only taking 3 days.</p>
-
 
+
-
successfully on different date. Device 1 took more time, about 7 days and was got on  
+
-
 
+
-
October 13th. Device 2 and 3 were transformed successfully on 10.14, only taking 3  
+
-
 
+
-
days.</p>
+
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>8.How much does it cost to acquire a set of measurements?</p>
<p>8.How much does it cost to acquire a set of measurements?</p>
-
<p> We did not calculate this carefully and we can just give a estimated value. We  
+
<p> We did not calculate this carefully and we can just give a estimated value. We cost about 1300RMB for this measurement, 800 for the reagent and 500 for the use of instrument.</p>
-
 
+
-
cost about 1300RMB for this measurement, 800 for the reagent and 500 for the use of  
+
-
 
+
-
instrument.</p>
+
<p>&nbsp;</p>
<p>&nbsp;</p>
-
<p>9.What are the practical limits on the number or rate of measurements taken with  
+
<p>9.What are the practical limits on the number or rate of measurements taken with this instrument and protocol?</p>
-
 
+
<p> pSB3K3 has a lower copy number so we had to cultured it for more times to select the colony of Device 1.<br />
-
this instrument and protocol?</p>
+
-
<p> pSB3K3 has a lower copy number so we had to cultured it for more times to select  
+
-
 
+
-
the colony of Device 1.<br />
+
</p>
</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
-
<div><strong><em><span lang="EN-US" xml:lang="EN-US">Section III: Measured  
+
<div><strong><em><span lang="EN-US" xml:lang="EN-US">Section III: Measured Quantities</span></em></strong></div>
-
 
+
-
Quantities</span></em></strong></div>
+
<p>
<p>
<p>
<p>
-
<p>1.For each type of quantity measured (e.g., fluorescence, optical density),  
+
<p>1.For each type of quantity measured (e.g., fluorescence, optical density), report on the following:
-
 
+
-
report on the following:
+
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>2.Units:</p>
<p>2.Units:</p>
<p>o  What are the units of the measurement? (e.g., meters, molecules)</p>
<p>o  What are the units of the measurement? (e.g., meters, molecules)</p>
<p> The number of light spot.</p>
<p> The number of light spot.</p>
-
<p>o  What is the equivalent unit expressed as a combination of the seven SI base  
+
<p>o  What is the equivalent unit expressed as a combination of the seven SI base units? (http://en.wikipedia.org/wiki/SI_base_unit)</p>
-
 
+
-
units? (http://en.wikipedia.org/wiki/SI_base_unit)</p>
+
<p> We just calculated the number, it does not need the SI unit.</p>
<p> We just calculated the number, it does not need the SI unit.</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>3.Precision: </p>
<p>3.Precision: </p>
-
<p>o  What is the range of possible measured values for this quantity, using your  
+
<p>o  What is the range of possible measured values for this quantity, using your instrument as configured for these measurements? (e.g., a meter stick measures in the range of 0 to 1 meter)</p>
-
 
+
<p> The spot number is always rational number while the RGB G value range is 0-255.</p>
-
instrument as configured for these measurements? (e.g., a meter stick measures in  
+
<p>o  What are the significant figures for these measurement? (e.g., on a meter stick, it is common to measure to the nearest millimeter).</p>
-
 
+
-
the range of 0 to 1 meter)</p>
+
-
<p> The spot number is always rational number while the RGB G value range is 0-
+
-
 
+
-
255.</p>
+
-
<p>o  What are the significant figures for these measurement? (e.g., on a meter  
+
-
 
+
-
stick, it is common to measure to the nearest millimeter).</p>
+
<p> Both the number and G value has the precision of 1.</p>
<p> Both the number and G value has the precision of 1.</p>
-
<p>o  Is the precision the same across the entire range? If not, how does it  
+
<p>o  Is the precision the same across the entire range? If not, how does it differ?</p>
-
 
+
-
differ?</p>
+
<p> Yes.</p>
<p> Yes.</p>
<p>o  How did you determine these answers?</p>
<p>o  How did you determine these answers?</p>
-
<p> Our method determined that we couldn't get a discrete result like 0.002. The  
+
<p> Our method determined that we couldn't get a discrete result like 0.002. The method is counting instead of detect.</p>
-
 
+
-
method is counting instead of detect.</p>
+
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>4.Accuracy:</p>
<p>4.Accuracy:</p>
Line 1,171: Line 793:
<p>
<p>
<p>
<p>
-
<div><strong><em><span lang="EN-US" xml:lang="EN-US">Section IV:  
+
<div><strong><em><span lang="EN-US" xml:lang="EN-US">Section IV: Measurements</span></em></strong></div>
-
 
+
-
Measurements</span></em></strong></div>
+
<p>
<p>
<p>
<p>
Line 1,183: Line 803:
       <span lang="EN-US" xml:lang="EN-US">o </span>
       <span lang="EN-US" xml:lang="EN-US">o </span>
       <!--[endif]-->
       <!--[endif]-->
-
       <em><span lang="EN-US" xml:lang="EN-US">the identity of the  
+
       <em><span lang="EN-US" xml:lang="EN-US">the identity of the sample</span></em></li>
-
 
+
-
sample</span></em></li>
+
   </ul>
   </ul>
</ul>
</ul>
-
<p><em><span lang="EN-US" xml:lang="EN-US">&nbsp;</span></em><strong><span lang="EN
+
<p><em><span lang="EN-US" xml:lang="EN-US">&nbsp;</span></em><strong><span lang="EN-US" xml:lang="EN-US">1-3:Device1; 4-6:Device2; 7-9:Device3.<em></em></span></strong></p>
-
 
+
-
-US" xml:lang="EN-US">1-3:Device1; 4-6:Device2; 7-
+
-
 
+
-
9:Device3.<em></em></span></strong></p>
+
<ul>
<ul>
   <ul>
   <ul>
Line 1,199: Line 813:
       <span lang="EN-US" xml:lang="EN-US">o </span>
       <span lang="EN-US" xml:lang="EN-US">o </span>
       <!--[endif]-->
       <!--[endif]-->
-
       <em><span lang="EN-US" xml:lang="EN-US">each quantity directly  
+
       <em><span lang="EN-US" xml:lang="EN-US">each quantity directly measured</span></em></li>
-
 
+
-
measured</span></em></li>
+
   </ul>
   </ul>
</ul>
</ul>
-
<p><strong><span lang="EN-US" xml:lang="EN-US">They are saved as picture in the .rar  
+
<p><strong><span lang="EN-US" xml:lang="EN-US">They are saved as picture in the .rar file.</span></strong></p>
-
 
+
-
file.</span></strong></p>
+
<ul>
<ul>
   <ul>
   <ul>
Line 1,213: Line 823:
       <span lang="EN-US" xml:lang="EN-US">o </span>
       <span lang="EN-US" xml:lang="EN-US">o </span>
       <!--[endif]-->
       <!--[endif]-->
-
       <em><span lang="EN-US" xml:lang="EN-US">each quantity derived from  
+
       <em><span lang="EN-US" xml:lang="EN-US">each quantity derived from measurements (e.g., fluorescence/OD)</span></em></li>
-
 
+
-
measurements (e.g., fluorescence/OD)</span></em></li>
+
   </ul>
   </ul>
</ul>
</ul>
-
<div><img src="http://2014.igem.org/wiki/images/7/71/LZUIMAGE6.png" alt=""  
+
<div><img src="http://2014.igem.org/wiki/images/7/71/LZUIMAGE6.png" alt="" width="654" height="272" /></div>
-
 
+
-
width="654" height="272" /></div>
+
<p>
<p>
-
<p><em><span lang="EN-US" xml:lang="EN-US">2.For each group of replicates,  
+
<p><em><span lang="EN-US" xml:lang="EN-US">2.For each group of replicates, report:</span></em>
-
 
+
-
report:</span></em>
+
<ul>
<ul>
   <ul>
   <ul>
Line 1,231: Line 835:
       <span lang="EN-US" xml:lang="EN-US">o </span>
       <span lang="EN-US" xml:lang="EN-US">o </span>
       <!--[endif]-->
       <!--[endif]-->
-
       <em><span lang="EN-US" xml:lang="EN-US">the identity of samples in the  
+
       <em><span lang="EN-US" xml:lang="EN-US">the identity of samples in the set</span></em></li>
-
 
+
-
set</span></em></li>
+
   </ul>
   </ul>
</ul>
</ul>
-
<p><em><span lang="EN-US" xml:lang="EN-
+
<p><em><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></em><strong><span lang="EN-US" xml:lang="EN-US">&nbsp;1-3:Device1; 4-6:Device2; 7-9:Device3.</span></strong><em><span lang="EN-US" xml:lang="EN-US"></span></em></p>
-
 
+
-
US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></em><strong><span  
+
-
 
+
-
lang="EN-US" xml:lang="EN-US">&nbsp;1-3:Device1; 4-6:Device2; 7-
+
-
 
+
-
9:Device3.</span></strong><em><span lang="EN-US" xml:lang="EN-US"></span></em></p>
+
<ul>
<ul>
   <ul>
   <ul>
Line 1,249: Line 845:
       <span lang="EN-US" xml:lang="EN-US">o </span>
       <span lang="EN-US" xml:lang="EN-US">o </span>
       <!--[endif]-->
       <!--[endif]-->
-
       <em><span lang="EN-US" xml:lang="EN-US">which, if any, of the samples are  
+
       <em><span lang="EN-US" xml:lang="EN-US">which, if any, of the samples are excluded and why</span></em></li>
-
 
+
-
excluded and why</span></em></li>
+
   </ul>
   </ul>
</ul>
</ul>
-
<p><em><span lang="EN-US" xml:lang="EN-US">&nbsp; </span></em><strong><span  
+
<p><em><span lang="EN-US" xml:lang="EN-US">&nbsp; </span></em><strong><span lang="EN-US" xml:lang="EN-US">No excluded sample.</span></strong><em><span lang="EN-US" xml:lang="EN-US"></span></em></p>
-
 
+
-
lang="EN-US" xml:lang="EN-US">No excluded sample.</span></strong><em><span lang="EN
+
-
 
+
-
-US" xml:lang="EN-US"></span></em></p>
+
<ul>
<ul>
   <ul>
   <ul>
Line 1,265: Line 855:
       <span lang="EN-US" xml:lang="EN-US">o </span>
       <span lang="EN-US" xml:lang="EN-US">o </span>
       <!--[endif]-->
       <!--[endif]-->
-
       <em><span lang="EN-US" xml:lang="EN-US">the mean and standard deviation for  
+
       <em><span lang="EN-US" xml:lang="EN-US">the mean and standard deviation for each quantity measured or derived</span></em></li>
-
 
+
-
each quantity measured or derived</span></em></li>
+
   </ul>
   </ul>
</ul>
</ul>
-
<div><img src="http://2014.igem.org/wiki/images/1/16/LZUIMAGE7.png" width="697"  
+
<div><img src="http://2014.igem.org/wiki/images/1/16/LZUIMAGE7.png" width="697" height="123" /></div>&nbsp;</p>
-
 
+
-
height="123" /></div>&nbsp;</p>
+
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
Line 1,287: Line 873:
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
-
<script type="text/javascript">
+
<p><script type="text/javascript">
var _bdhmProtocol = (("https:" == document.location.protocol) ? " https://" : " http://");
var _bdhmProtocol = (("https:" == document.location.protocol) ? " https://" : " http://");
document.write(unescape("%3Cscript src='" + _bdhmProtocol + "hm.baidu.com/h.js%3F986011a32f84f432107ce07502765e79' type='text/javascript'%3E%3C/script%3E"));
document.write(unescape("%3Cscript src='" + _bdhmProtocol + "hm.baidu.com/h.js%3F986011a32f84f432107ce07502765e79' type='text/javascript'%3E%3C/script%3E"));
</script>
</script>
-
 
</p>
</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
Line 1,317: Line 902:
   <table width="1365" border="0"">
   <table width="1365" border="0"">
     <tr>
     <tr>
-
       <td background="http://2014.igem.org/wiki/images/a/a6/Huils.jpg" width="1365"  
+
       <td background="http://2014.igem.org/wiki/images/a/a6/Huils.jpg" width="1365" height="463"><img src="http://2014.igem.org/wiki/images/0/07/Dblbluebar.jpg" width="1365" height="463" /></td>
-
 
+
-
height="463"><img src="http://2014.igem.org/wiki/images/0/07/Dblbluebar.jpg"  
+
-
 
+
-
width="1365" height="463" /></td>
+
     </tr>
     </tr>
   </table>
   </table>
</div>
</div>
-
<div id="apDiv6"><img src="http://2014.igem.org/wiki/images/6/6e/Lzulogo.png"  
+
<div id="apDiv6"><img src="http://2014.igem.org/wiki/images/6/6e/Lzulogo.png" width="220" height="221" /></div>
-
 
+
-
width="220" height="221" /></div>
+
<div >
<div >
   <div style=" height:890px;"> </div>
   <div style=" height:890px;"> </div>
Line 1,346: Line 925:
<a class="logo" href="http://2014.igem.org/Team:LZU-China"></a>
<a class="logo" href="http://2014.igem.org/Team:LZU-China"></a>
<div class="navs">
<div class="navs">
-
<a class="def-nav" href="http://2014.igem.org/Team:LZU-
+
<a class="def-nav" href="http://2014.igem.org/Team:LZU-China">Home</a>
-
 
+
-
China">Home</a>
+
<span class="separate"></span>
<span class="separate"></span>
<div class="def-nav current has-pulldown-special">
<div class="def-nav current has-pulldown-special">
-
<a class="pulldown-nav" href=""><em class="f-
+
<a class="pulldown-nav" href=""><em class="f-icon"></em>Project</a>
-
 
+
-
icon"></em>Project</a>
+
<div class="pulldown app-url">
<div class="pulldown app-url">
<div class="content">
<div class="content">
-
<a class="li disk"  
+
<a class="li disk" href="http://2014.igem.org/Team:LZU-China/background">
-
 
+
-
href="http://2014.igem.org/Team:LZU-China/background">
+
<span class="icon"></span>
<span class="icon"></span>
<span class="text">Background</span>
<span class="text">Background</span>
</a>
</a>
-
<a class="li tongxunlu"  
+
<a class="li tongxunlu" href="http://2014.igem.org/Team:LZU-China/wetlab">
-
 
+
-
href="http://2014.igem.org/Team:LZU-China/wetlab">
+
<span class="icon"></span>
<span class="icon"></span>
<span class="text">Wet Lab</span>
<span class="text">Wet Lab</span>
</a>
</a>
-
<a class="li xiangce"  
+
<a class="li xiangce" href="http://2014.igem.org/Team:LZU-China/drylab">
-
 
+
-
href="http://2014.igem.org/Team:LZU-China/drylab">
+
<span class="icon"></span>
<span class="icon"></span>
<span class="text">Dry Lab</span>
<span class="text">Dry Lab</span>
</a>
</a>
-
<a class="li note"  
+
<a class="li note" href="http://2014.igem.org/Team:LZU-China/Parts">
-
 
+
-
href="http://2014.igem.org/Team:LZU-China/Parts">
+
<span class="icon"></span>
<span class="icon"></span>
<span class="text">Parts</span>
<span class="text">Parts</span>
</a>
</a>
-
                     <a class="li duanxin" href="http://2014.igem.org/Team:LZU-
+
                     <a class="li duanxin" href="http://2014.igem.org/Team:LZU-China/interlab">
-
 
+
-
China/interlab">
+
<span class="icon"></span>
<span class="icon"></span>
<span class="text">Interlab</span>
<span class="text">Interlab</span>
</a>
</a>
-
<a class="li zhaohui"  
+
<a class="li zhaohui" href="http://2014.igem.org/Team:LZU-China/future">
-
 
+
-
href="http://2014.igem.org/Team:LZU-China/future">
+
<span class="icon"></span>
<span class="icon"></span>
-
<span class="text">Future  
+
<span class="text">Future Work</span>
-
 
+
-
Work</span>
+
</a>
</a>
</div>
</div>
Line 1,398: Line 959:
</div>
</div>
     <span class="separate"></span>
     <span class="separate"></span>
-
<a class="def-nav" href="http://2014.igem.org/Team:LZU-
+
<a class="def-nav" href="http://2014.igem.org/Team:LZU-China/Notebook">Note Book</a>
-
 
+
-
China/Notebook">Note Book</a>
+
         <span class="separate"></span>
         <span class="separate"></span>
-
<a class="def-nav" href="http://2014.igem.org/Team:LZU-
+
<a class="def-nav" href="http://2014.igem.org/Team:LZU-China/Safety">Safety</a>
-
 
+
-
China/Safety">Safety</a>
+
         <span class="separate"></span>
         <span class="separate"></span>
-
<a class="def-nav" href="http://2014.igem.org/Team:LZU-China/hp">H.  
+
<a class="def-nav" href="http://2014.igem.org/Team:LZU-China/hp">H. Practice</a>
-
 
+
-
Practice</a>
+
</div>
</div>
<div class="info">
<div class="info">
Line 1,414: Line 969:
<li class="info-i user-name has-pulldown">
<li class="info-i user-name has-pulldown">
<em class="f-icon pull-arrow"></em>
<em class="f-icon pull-arrow"></em>
-
<a class="f-icon identity"  
+
<a class="f-icon identity" href="http://2014.igem.org/Team:LZU-China/Team" title="Our team"></a>
-
 
+
-
href="http://2014.igem.org/Team:LZU-China/Team" title="Our team"></a>
+
<span class="name top-username">Our Team</span>
<span class="name top-username">Our Team</span>
<div class="pulldown user-info">
<div class="pulldown user-info">
<em class="arrow"></em>
<em class="arrow"></em>
<div class="content">
<div class="content">
-
<span class="li"><a href="http://2014.igem.org/Team:LZU-China/Team">Team  
+
<span class="li"><a href="http://2014.igem.org/Team:LZU-China/Team">Team Members</a></span>
-
 
+
<span class="li"><a href="http://2014.igem.org/Team:LZU-China/Instructors">Advisors</a></span>
-
Members</a></span>
+
<span class="li"><a href="http://2014.igem.org/Team:LZU-China/Instructors">Instructors</a></span>
-
<span class="li"><a href="http://2014.igem.org/Team:LZU-
+
-
 
+
-
China/Instructors">Advisors</a></span>
+
-
<span class="li"><a href="http://2014.igem.org/Team:LZU-
+
-
 
+
-
China/Instructors">Instructors</a></span>
+
                         <span class="separate-li no-height"></span>
                         <span class="separate-li no-height"></span>
-
<span class="li"><a href="http://2014.igem.org/Team:LZU-
+
<span class="li"><a href="http://2014.igem.org/Team:LZU-China/Attribution">Attribution</a></span>
-
 
+
<span class="li"><a href="http://2014.igem.org/Team:LZU-China/acknowledgement">Acknowledgement</a></span>
-
China/Attribution">Attribution</a></span>
+
<span class="separate-li no-height"></span>
-
<span class="li"><a href="http://2014.igem.org/Team:LZU-
+
-
 
+
-
China/acknowledgement">Acknowledgement</a></span>
+
-
<span class="separate-li no-
+
-
 
+
-
height"></span>
+
</div>
</div>
</div>
</div>
Line 1,449: Line 990:
<em class="arrow"></em>
<em class="arrow"></em>
<div class="content">
<div class="content">
-
<span class="separate-li no-
+
<span class="separate-li no-height"></span>
-
 
+
<span class="li"><a href="">Team Profile</a></span>
-
height"></span>
+
<span class="li"><a href="http://en.lzu.edu.cn/">Lanzhou University</a></span>
-
<span class="li"><a href="">Team  
+
<span class="li"><a href="http://2013.igem.org/Team:LZU-China">LZU-CHINA 2013</a></span>
-
 
+
-
Profile</a></span>
+
-
<span class="li"><a  
+
-
 
+
-
href="http://en.lzu.edu.cn/">Lanzhou University</a></span>
+
-
<span class="li"><a  
+
-
 
+
-
href="http://2013.igem.org/Team:LZU-China">LZU-CHINA 2013</a></span>
+
</div>
</div>
</div>
</div>
Line 1,467: Line 1,000:
</div>
</div>
</div>
</div>
-
<div style="text-align:center;margin:50px 0; font:normal 14px/24px 'MicroSoft  
+
<div style="text-align:center;margin:50px 0; font:normal 14px/24px 'MicroSoft YaHei';">
-
 
+
-
YaHei';">
+
</div>
</div>
Line 1,488: Line 1,019:
   var i,p,v,obj,args=MM_showHideLayers.arguments;
   var i,p,v,obj,args=MM_showHideLayers.arguments;
   for (i=0; i<(args.length-2); i+=3)  
   for (i=0; i<(args.length-2); i+=3)  
-
   with (document) if (getElementById && ((obj=getElementById(args[i]))!=null)) {  
+
   with (document) if (getElementById && ((obj=getElementById(args[i]))!=null)) { v=args[i+2];
-
 
+
     if (obj.style) { obj=obj.style; v=(v=='show')?'visible':(v=='hide')?'hidden':v; }
-
v=args[i+2];
+
-
     if (obj.style) { obj=obj.style; v=(v=='show')?'visible':(v=='hide')?'hidden':v;  
+
-
 
+
-
}
+
     obj.visibility=v; }
     obj.visibility=v; }
}
}
</script>
</script>

Revision as of 20:53, 17 October 2014

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> LZU-China 2014

 
 
 

 

Our Interlab

 

       In our interlab work we test the three required parts. They are:

 

  •               1. BBa_I20260 (J23101-B0032-E0040-B0015) Plate 4,Well 18A
  •               2. BBa_J23101 + BBa_E0240 (B0032-E0040-B0015) Plate 1,Well 20K and Plate 2,Well 24B 

           3.BBa_J23115 + BBa_E0240 (B0032-E0040-B0015) Plate 1,Well 22I and Plate 2,Well 24B

 

 

 

 

 

The gel picture of each device

 

 

 

                                          Figure-1 The gel picture of each device

                                                                   Table-1 Each lane’s properties

 

 

Protocol

 

Equipment and chemicals

   20μL eppendorf tubes x9

   ddH2O

   Competent E.Coli

   The devices

   Fluorescence microscope.

Methods:

   Each device was transformed in competent E.Coli and shaken cultured at 37℃ for 18h. Then we detected the number of green light spot by software. The final result is the average light spot , which are more bright than G value 192 for 3 random sites of each site by the algorithm which we used to developed.

 

 

 

Result

 

 

 

            Picture 1-3 are the device 1's results, Picture 4-6 are the device 2's and the Picture 7-9 are the device 3's. Each picture is 400x and we calculated the light spot. The result are listed here:

 

                                                 Figure-3 The "light" spot number of each devices

 

                                                  Table-3 The mean and standard deviation of each device

 

                     Besides, we used the cytoflower to detect the number of cells with green fluorescence. The result is here: (Tube 001 is the control. Tube 002 and 003 are Device 2 . Tube 004 is Device 1 and Tube 005 is Device 3. )

 

 

 

 

 

Section I: Provenance & Release

 

 

1.Who did the actual work to acquire these measurements?

Qi Wu, Hongxia Zhao and Ruixue Zhao.

 

2.What other people should be credited for these measurements? (i.e., who would be an author on any resulting publication. For example, your faculty advisor may have helped design the protocols that you ran.)

Xiangkai Li will be the "corresponding author".

 

3.On what dates were the protocols run and the measurements taken? (this will often be a range of dates; make sure you say which data was taken at what times.)

We ran the protocol on2014.10.4 because of the mistake and we took the simple fluorescence data on 2014.10.16. The data made by flow cytometer was got on 2014.10.17.

 

4.Do all persons involved consent to the inclusion of this data in publications derived from the iGEM interlab study?

Yes.

 

 

Section II: Protocol

 

 

1.What protocol did you use to prepare samples for measurement?

We used the Biobrick protocol to assembly and the strain was DH5 Alpha.

 

2.What sort of instrument did you use to acquire measurements?

o What is the model and manufacturer?

Fluorescence microscope is Nikon OPTIPHOT-2 .

o How is it configured for your measurements? (e.g., light filters, illumination, amplification)

The microscope's exciting light was set as 492nm.

 

3.What protocol did you use to take measurements?

The transformed E coli. Was shaken cultured about 17h and we took photo by the fluorescence microscope.Then we analyzed the number of light spots by software.

 

4.What method is used to determine whether to include or exclude each sample from the data set?

After the scoping we did electrophoresis for each tube to exam if the bacteria had been transformed.

 

5.What exactly were the controls that you used?

DH5 Alpha without transforming which had been shaken cultured for 17h.

 

6.What quantities were measured? (e.g., red fluorescence, green fluorescence, optical density)

Green fluorescence. For the three reporters are all GFP.

 

7.How much time did it take to acquire each set of measurements?

The data was got on the same day. While different device was transformed successfully on different date. Device 1 took more time, about 7 days and was got on October 13th. Device 2 and 3 were transformed successfully on 10.14, only taking 3 days.

 

8.How much does it cost to acquire a set of measurements?

We did not calculate this carefully and we can just give a estimated value. We cost about 1300RMB for this measurement, 800 for the reagent and 500 for the use of instrument.

 

9.What are the practical limits on the number or rate of measurements taken with this instrument and protocol?

pSB3K3 has a lower copy number so we had to cultured it for more times to select the colony of Device 1.

 

 

       

Section III: Measured Quantities

1.For each type of quantity measured (e.g., fluorescence, optical density), report on the following:

 

2.Units:

o What are the units of the measurement? (e.g., meters, molecules)

The number of light spot.

o What is the equivalent unit expressed as a combination of the seven SI base units? (http://en.wikipedia.org/wiki/SI_base_unit)

We just calculated the number, it does not need the SI unit.

 

3.Precision:

o What is the range of possible measured values for this quantity, using your instrument as configured for these measurements? (e.g., a meter stick measures in the range of 0 to 1 meter)

The spot number is always rational number while the RGB G value range is 0-255.

o What are the significant figures for these measurement? (e.g., on a meter stick, it is common to measure to the nearest millimeter).

Both the number and G value has the precision of 1.

o Is the precision the same across the entire range? If not, how does it differ?

Yes.

o How did you determine these answers?

Our method determined that we couldn't get a discrete result like 0.002. The method is counting instead of detect.

 

4.Accuracy:

o When was the instrument last calibrated?

2014.8.11.

o How was the instrument calibrated?

Calibrating the light source by raster correction

Section IV: Measurements

1.For each sample, report:

    • o the identity of the sample

 1-3:Device1; 4-6:Device2; 7-9:Device3.

    • o each quantity directly measured

They are saved as picture in the .rar file.

    • o each quantity derived from measurements (e.g., fluorescence/OD)

2.For each group of replicates, report:

    • o the identity of samples in the set

           1-3:Device1; 4-6:Device2; 7-9:Device3.

    • o which, if any, of the samples are excluded and why

  No excluded sample.

    • o the mean and standard deviation for each quantity measured or derived