Team:LZU-China/drylab

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      <td width="50%"><p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<span lang="EN-US" xml:lang="EN-US">The LZU-china iGEM 2014 team consists of 13 dedicated undergraduate students forming a large        family. This year, it is our seconed year to attend iGEM. There is no doubt that we are all        enthusiastic for researches and experiments. All of us have been looking        forward to understanding more about life sciences.</span></p>
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        <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;What's more there are also 4 instructors and 4 advisers in our team. With their instruction and advice, We can translate our ideas  into reality much easier. </p>
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  <div><a href="https://2014.igem.org/Team:LZU-China/Team"><img src="https://static.igem.org/mediawiki/2014/8/8d/Z_01.png" alt="" width="228" height="73"></a><a href="https://2014.igem.org/Team:LZU-China/Instructors"><img src="https://static.igem.org/mediawiki/2014/e/ee/Z_02.png" alt="" width="213" height="73"></a><a href="https://2014.igem.org/Team:LZU-China/Attribution"><img src="https://static.igem.org/mediawiki/2014/6/6d/Z_03.png" alt="" width="212" height="73"></a><a href="https://2014.igem.org/Team:LZU-China/acknowledgement"><img src="https://static.igem.org/mediawiki/2014/c/c1/Z_04.png" alt="" width="287" height="73"></a></div>
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      <div>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;DRY LAB WORK</div>
 
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      <div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;ACKNOWLEDGEMENT</div>
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          <td>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In dry lab work, we used the work of wet lab experiments, established an entire system whose function is real-time monitoring the condition of MFCs. Our system can remotely show the output voltage of MFC, thereby automatically calculate the current PNP concentration. The detailed description of dry lab work is as following.</td>
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             <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This project is supported by School of Life Science and Cuiying Honors College, Lanzhou University, MOST international cooperation funds, 2014DFA91340, Gansu provincial international cooperation funds, 1304WCGA176, Majorbio Cooperation and Takara China.</p>
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          <td background="huils.jpg" height="60"><div>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;DETAILED INFOMATION</div></td>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;First of all, in order to accurately measure the output voltage of MFCs, we looked for appropriate voltmeter on the market and gave them an extensive evaluation. The final choice is UNI-T UT800 Series of high precision digital bench-type multimeter because of its accurate performance (Resolution 0.1mV). Then, we used Z-TEK RS232 to USB cable to link the voltage meters to the computer.</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2014/a/a8/UT-803.png" width="238" height="190" />&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2014/9/96/Usbcom.png" width="200" height="185" /></p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;Fig.1 UT-803 multimeter&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Fig.2 RS232 to USB cable</p>
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We use software LabView to develop our program: this program based on the COM Port Aggregation Protocol from the voltmeter reads the output voltage of the current MFC device in real time and then puts the value into empirical formula (the relationship between output voltage and PNP concentration was tested and concluded by the experiment before)to calculate current PNP substrate concentrations.</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2014/6/63/App1.png" width="328" height="582" />&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2014/5/5d/App0.png" width="328" height="582" />&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Fig.3 Program interface (welcome) &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Fig.4 Program interface (connection) </p>
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In addition, our program can also fabricate the curve based on the PNP--voltage empirical formula, and note the measured values point based on real-time scanning. It is noticeable that, our program also show an incredible compatibility. If the user had known the true value of PNP substrate concentration, then he could easily put a note into the source code in order to correct the false point.</p>
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            <p>&nbsp;</p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2014/b/be/App2.png" width="328" height="582" />&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2014/1/18/Lvcode.png" width="450" height="359" /></p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Fig.5 Program interface (make chart) &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Fig.6 Source code (partial) </p>
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Currently, we use the telnet program developed by third party to run our program in smartphones. So we can monitor our MFC device whenever and wherever we are. So we can monitor our MFCs device whenever and wherever we are. </p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="1mfc.jpg" width="200" height="150" />&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="2cmp.png" width="200" height="150" />&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="3phone.png" width="100" height="204" /></p>
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            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Fig. 7 The automatic monitor system (sketch map)</p>
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In the future, we will develop a new version of our app (may be published at the end of 2014), our program will have multiple modes for users to choose, in the &quot;calibrate&quot; mode, it could be possible for the user to add new data in order to calibrate curves and even gets the more accurate  formula without changing the source code. In addition, We are trying to develop a real app which can run in the Android system without any other Telnet program.<br />
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                <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg" width="30%"><a href="http://en.lzu.edu.cn/"><img src="https://static.igem.org/mediawiki/2014/6/6e/Lzulogo.png" alt="" width="220" height="221"></a></td>
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                      <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg">Lanzhou University</td>
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                  <p>&nbsp;</p>
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                  <p><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span><span lang="EN-US" xml:lang="EN-US">Lanzhou University has been the best university in the northwestern region of China, ranking as one of the top 30 among over 1,500 universities across China. It is one of the key universities administered directly by the Ministry of Education.</span></p>
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                      <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg"><a href="http://en.lzu.edu.cn/"><img src="https://static.igem.org/mediawiki/2014/7/70/More.png" width="172" height="40"></a></td>
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                  <p>&nbsp;</p></td>
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                      <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg">School of Life Sciences</td>
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                  <p>&nbsp;</p>
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                  <p><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The School of Life Sciences at Lanzhou University has a long history. It originated as the Department of Botany and the Department of Zoology of National Lanzhou University, which were founded in 1946 as the first departments of life sciences in western China. After several generations' efforts in over sixty years, the School of Life Science is in a good development trend in many aspects, such as faculty and staff, discipline construction, teaching and researching, and local service. Basing its scientific research on the cold and arid environment in western China, the faculty has made great achievements. The School of Life Sciences is active in international collaboration in order to create an open and supportive academic atmosphere, extend academic horizons, and improve the faculty and students' ability in research and creativity.</span></p>
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                      <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg"><a href="http://lifesc.lzu.edu.cn:81/en/"><img src="https://static.igem.org/mediawiki/2014/7/70/More.png" width="172" height="40"></a></td>
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                  <p></p></td>
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                <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg" width="26%"><p><a href="http://lifesc.lzu.edu.cn:81/en/"><img src="https://static.igem.org/mediawiki/2014/e/e1/LifenewsLogo.png" alt="" width="223" height="220"></a></p></td>
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                    <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg">Cuiying Honors College</td>
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                  <p>&nbsp;</p>
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                  <p><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cuiying Honors College (CHC) was founded in August 2010 within Lanzhou University as part of China's Top-Notch Undergraduate Training Program. The Ministry of Education and the Ministry of Finance are investing in this new pilot scheme in order to develop future leaders with exceptional talent in the core disciplines. Every year this program only admits the best 1000 students from the 6 million new undergraduates nationwide. Lanzhou University has been selected as one of 19 institutions implementing this national program.</span></p>
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                      <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg"><a href="http://chc.lzu.edu.cn/en/"><img src="https://static.igem.org/mediawiki/2014/7/70/More.png" alt="" width="172" height="40"></a></td>
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                <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg" width="26%"><p><a href="http://chc.lzu.edu.cn/en/"><img src="https://static.igem.org/mediawiki/2014/d/d3/Cuiying_honor_college.png" alt="" width="220" height="220"></a></p></td>
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                    <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg">Takara Dalian</td>
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                  </tr>
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                </tbody></table>
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                  <p>&nbsp;</p>
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                  <p><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>Takara Biotechnology (Dalian) Co., Ltd. (Takara Dalian), located in Dalian Economic and Technical Development Zone, is a subsidiary of Takara Bio Inc.(TBI), the leading Japanese manufacturer of reagents for use in genetic engineering research. Takara Bio Inc.’s products are much in demand around the world.&nbsp;</p>
 +
                  <table width="100%" border="0">
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                      <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg" width="65%" height="50">&nbsp;</td>
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                      <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg"><a href="http://www.takara.com.cn/"><img src="https://static.igem.org/mediawiki/2014/7/70/More.png" alt="" width="172" height="40"></a></td>
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                    </tr>
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                  </tbody></table>
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                  <p>&nbsp;</p></td>
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              </tr>
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            </tbody></table>
             <p>&nbsp;</p>
             <p>&nbsp;</p>
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            <table width="100%" border="0">
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              <tbody><tr>
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                <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg" width="30%">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="http://www.majorbio.com/"><img src="https://static.igem.org/mediawiki/2014/c/c0/Majorbio.png" alt="" width="180" height="65"></a></td>
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                <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg" width="76%"><table width="100%" border="0">
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                    <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg">Majorbio</td>
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                  </tr>
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                </tbody></table>
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                  <p>&nbsp;</p>
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                  <p><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>Majorbio(Shanghai) Co., Ltd. is a company focusing on the high-throughput sequencing technology, bioinformatics analysis, and clinical diagnostic kits development. So far Majorbio has build three experiment bases in Shanghai,  Beijing and Guangzhou.</p>
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                  <table width="100%" border="0">
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                    <tbody><tr>
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                      <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg" width="65%" height="50">&nbsp;</td>
 +
                      <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg"><a href="http://www.majorbio.com/"><img src="https://static.igem.org/mediawiki/2014/7/70/More.png" alt="" width="172" height="40"></a></td>
 +
                    </tr>
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                  </tbody></table>
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                  <p>&nbsp;</p></td>
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           <td background="https://static.igem.org/mediawiki/2014/thumb/a/a6/Huils.jpg/800px-Huils.jpg" width="10%">&nbsp;</td>
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      <p><span lang="EN-US" xml:lang="EN-US"><span lang="EN-US" xml:lang="EN-US">&nbsp;</span><span lang="EN-US" xml:lang="EN-US">&nbsp;<a href="https://2014.igem.org/Team:LZU-China/Attribution"><img src="Attributionb.png" alt="" width="388" height="90"></a>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;</span>&nbsp;&nbsp;</span><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span><span lang="EN-US" xml:lang="EN-US"><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;</span>&nbsp;&nbsp;</span><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span><span lang="EN-US" xml:lang="EN-US">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></p>
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      <p>&nbsp;</p>
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Revision as of 00:49, 16 October 2014

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> LZU-China 2014

 
 

 

 

          The LZU-china iGEM 2014 team consists of 13 dedicated undergraduate students forming a large family. This year, it is our seconed year to attend iGEM. There is no doubt that we are all enthusiastic for researches and experiments. All of us have been looking forward to understanding more about life sciences.

          What's more there are also 4 instructors and 4 advisers in our team. With their instruction and advice, We can translate our ideas into reality much easier.

 

 

  

 

 

 

 

 

        ACKNOWLEDGEMENT

 

 

 

       This project is supported by School of Life Science and Cuiying Honors College, Lanzhou University, MOST international cooperation funds, 2014DFA91340, Gansu provincial international cooperation funds, 1304WCGA176, Majorbio Cooperation and Takara China.

 

 

Lanzhou University

 

           Lanzhou University has been the best university in the northwestern region of China, ranking as one of the top 30 among over 1,500 universities across China. It is one of the key universities administered directly by the Ministry of Education.

 

 

 
School of Life Sciences

 

          The School of Life Sciences at Lanzhou University has a long history. It originated as the Department of Botany and the Department of Zoology of National Lanzhou University, which were founded in 1946 as the first departments of life sciences in western China. After several generations' efforts in over sixty years, the School of Life Science is in a good development trend in many aspects, such as faculty and staff, discipline construction, teaching and researching, and local service. Basing its scientific research on the cold and arid environment in western China, the faculty has made great achievements. The School of Life Sciences is active in international collaboration in order to create an open and supportive academic atmosphere, extend academic horizons, and improve the faculty and students' ability in research and creativity.

 

Cuiying Honors College

 

            Cuiying Honors College (CHC) was founded in August 2010 within Lanzhou University as part of China's Top-Notch Undergraduate Training Program. The Ministry of Education and the Ministry of Finance are investing in this new pilot scheme in order to develop future leaders with exceptional talent in the core disciplines. Every year this program only admits the best 1000 students from the 6 million new undergraduates nationwide. Lanzhou University has been selected as one of 19 institutions implementing this national program.

 

 

Takara Dalian

 

           Takara Biotechnology (Dalian) Co., Ltd. (Takara Dalian), located in Dalian Economic and Technical Development Zone, is a subsidiary of Takara Bio Inc.(TBI), the leading Japanese manufacturer of reagents for use in genetic engineering research. Takara Bio Inc.’s products are much in demand around the world. 

 

 

 

            
Majorbio

 

           Majorbio(Shanghai) Co., Ltd. is a company focusing on the high-throughput sequencing technology, bioinformatics analysis, and clinical diagnostic kits development. So far Majorbio has build three experiment bases in Shanghai, Beijing and Guangzhou.

 

 

 

 

 

 

  

                                                                                                    

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Retrieved from "http://2014.igem.org/Team:LZU-China/drylab"