Team:LZU-China/Safety

From 2014.igem.org

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<br>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:LZU-China/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:LZU-China/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:LZU-China/Attributions"style="color:#000000"> Attributions </a></td>
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<tr><td > <h3> Welcome! </h3></td>
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<td > <h3> Timeline</h3></td>
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<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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<h3> Edit this page!</h3>
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Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
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<ul>
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<li> <a href=" ">Learn about lab Safety for Today</a></li>
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<li> <a href="">Learn about Safety for the future of your project.</a></li>
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-->
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<h3> Your Lab </h3>
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<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>
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Image:Example2_Lab_1.png|The building our lab is in!
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Image:Example2_Lab_4.png|Working in biosafety cabinets
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Image:Example2_Lab_5.png|Team all gloved up and ready for work!
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<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
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<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
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<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
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<li><b>June 9: </b>Submit the About Our Lab form.</li>
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<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
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<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
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<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
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<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
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<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
+
-
<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
+
-
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<div id="apDiv2">
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  <table width="100%" border="0" >
 +
    <tr>
 +
      <td width="1201" height="265" rowspan="2"><p><img src="https://static.igem.org/mediawiki/2014/d/de/6members.top.jpg" width="1366" height="270" /></p></td>
 +
      <td width="100%" height="199" bgcolor="#187ADB">&nbsp;</td>
 +
    </tr>
 +
    <tr>
 +
      <td height="70">&nbsp;</td>
 +
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 +
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 +
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 +
<div id="apDiv3">
 +
  <div>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;SAFETY</div>
 +
  <p>&nbsp;</p>
 +
  <table width="100%" border="0">
 +
    <tr>
 +
      <td width="22%">&nbsp;</td>
 +
      <td width="56%"><p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
 +
        <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
 +
        <table width="750" height="358" border="1" align="center" cellpadding="0" cellspacing="0">
 +
          <tr>
 +
            <td width="18"><p align="center"><strong>&nbsp;</strong></p></td>
 +
            <td width="89"><p align="center"><strong>Species</strong></p></td>
 +
            <td width="67"><p align="center"><strong>Strain    no/name</strong></p></td>
 +
            <td width="48"><p align="center"><strong>Risk Group</strong></p></td>
 +
            <td width="516"><p align="center"><strong>Disease    risk to humans? If so, which disease</strong></p></td>
 +
          </tr>
 +
          <tr>
 +
            <td><p align="left">&nbsp;1</p></td>
 +
            <td><p align="left"><em>E. coli</em>K 12</p></td>
 +
            <td><p align="left">DH5 alpha</p></td>
 +
            <td><p align="left"><a href="http://www.absa.org/riskgroups/bacteriasearch.php?genus=&amp;species=coli">1</a></p></td>
 +
            <td><p align="left">Yes. May    cause irritation to skin, eyes, and respiratory tract, may affect kidneys.    However, E. coli K-12 is not considered a human or animal pathogen nor is it    toxicogenic. Any concerns for E. coli K-12 in terms of health considerations are mitigated    by its poor ability to colonize the colon and establish infections.</p></td>
 +
          </tr>
 +
          <tr>
 +
            <td><p align="left">&nbsp;2</p></td>
 +
            <td><p align="left"><em>Shewenella    Onedensis </em></p></td>
 +
            <td><p align="left">MR1</p></td>
 +
            <td><p align="left"><a href="http://www.absa.org/riskgroups/bacteriasearch.php?genus=&amp;species=coli">1</a></p></td>
 +
            <td><p align="left">No</p></td>
 +
          </tr>
 +
        </table>
 +
        <p>&nbsp;</p>
 +
        <p>&nbsp;</p>
 +
        <table width="750" border="1" align="center" cellpadding="0" cellspacing="0">
 +
          <tr>
 +
            <td width="15"><p align="center"><strong>&nbsp;</strong></p></td>
 +
            <td width="96"><p align="center"><strong>Part    number</strong></p></td>
 +
            <td width="131"><p align="center"><strong>Where did    us get the physical DNA for this part</strong></p></td>
 +
            <td width="121"><p align="center"><strong>What    species does this part originally come from?</strong></p></td>
 +
            <td width="96" valign="top"><p align="center"><strong>&nbsp;</strong></p>
 +
              <p align="center"><strong>Natural    function of part</strong></p></td>
 +
            <td width="70"><p align="center"><strong>What is    the Risk Group of the species?</strong></p></td>
 +
            <td width="130"><p align="center"><strong>How will    you use it?</strong></p></td>
 +
          </tr>
 +
          <tr>
 +
            <td width="15"><p align="left">&nbsp;1</p></td>
 +
            <td width="120"><p align="left">pSB1C3</p></td>
 +
            <td width="131"><p align="left">From Li    Xiangkai's Lab, Lanzhou    University</p></td>
 +
            <td width="121"><p align="left"><em>Escherichia coli</em> K-12</p></td>
 +
            <td width="96" valign="top"><p align="left">Vector for <em>Escherichia coli</em></p></td>
 +
            <td width="51"><p align="left">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1</p></td>
 +
            <td width="130"><ol>
 +
              <li>Vector for <em>E.coli </em>to express BBA_K1523101</li>
 +
              <li>Submission    vector for all parts </li>
 +
            </ol></td>
 +
          </tr>
 +
          <tr>
 +
            <td width="15"><p align="left">&nbsp;2</p></td>
 +
            <td width="96"><p align="left">BBA_K1523101</p></td>
 +
            <td width="131"><p align="left">From    distributed kit of iGEM 2013<br />
 +
              BBA&shy;_K381001</p></td>
 +
            <td width="121"><p align="left"><em>Escherichia coli</em> K-12 </p></td>
 +
            <td width="96" valign="top"><p align="left">Detector    of Nitrate</p></td>
 +
            <td width="51"><p align="left">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1</p></td>
 +
            <td width="130"><p align="left">1.&nbsp; Strain used in anode of MFC 2.&nbsp; Detector of PNP<br />
 +
              3.&nbsp; Riboflavin producing</p></td>
 +
          </tr>
 +
          <tr>
 +
            <td width="15"><p align="left">&nbsp;3</p></td>
 +
            <td width="96"><p align="left">pET-28(a)    +</p></td>
 +
            <td width="131"><p align="left">From Li    Xiangkai's Lab, Lanzhou    University</p></td>
 +
            <td width="121"><p align="left">Vector for <em>Escherichia coli</em></p></td>
 +
            <td width="96" valign="top"><p align="left">Vector for <em>Escherichia coli</em></p></td>
 +
            <td width="51"><p align="left">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1</p></td>
 +
            <td width="130"><p align="left">Vector for <em>E.coli</em> to express BBA_K1523101</p></td>
 +
          </tr>
 +
          <tr>
 +
            <td width="15"><p align="left">&nbsp;4</p></td>
 +
            <td width="96"><p align="left">BBa_K1523008</p></td>
 +
            <td width="131"><p align="left">Lanzhou University </p></td>
 +
            <td width="121"><p align="left"><em>Escherichia coli</em></p></td>
 +
            <td width="96" valign="top"><p align="left">Chromate    reduction</p></td>
 +
            <td width="51"><p align="left">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1</p></td>
 +
            <td width="130" valign="top"><p align="left">1.&nbsp; Strain used in anode of MFC 2.&nbsp; Chromate reduction in anode</p></td>
 +
          </tr>
 +
          <tr>
 +
            <td width="15"><p align="left">&nbsp;5</p></td>
 +
            <td width="96"><p align="left">BBa_K1523021</p></td>
 +
            <td width="131"><p align="left">Lanzhou University </p></td>
 +
            <td width="121"><p align="left"><em>Pseudomonas fluorescens</em></p></td>
 +
            <td width="96" valign="top"><p align="left">Chromate    reduction</p></td>
 +
            <td width="51"><p align="left">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1</p></td>
 +
            <td width="130" valign="top"><p align="left">1.&nbsp; Strain used in cathode of MFC <br />
 +
              2.&nbsp; Other organic pollutant reduction in    cathode</p></td>
 +
          </tr>
 +
          <tr>
 +
            <td width="15"><p align="left">&nbsp;6</p></td>
 +
            <td width="96"><p align="left">BBa_K1523022</p></td>
 +
            <td width="131"><p align="left">Lanzhou University </p></td>
 +
            <td width="121"><p align="left"><em>Pseudomonas fluorescens</em></p></td>
 +
            <td width="96" valign="top"><p align="left">Chromate    reduction</p></td>
 +
            <td width="51"><p align="left">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1</p></td>
 +
            <td width="130" valign="top"><p align="left">1.&nbsp; Strain used in cathode of MFC <br />
 +
              2.&nbsp; Other organic pollutant reduction in    cathode</p></td>
 +
          </tr>
 +
          <tr>
 +
            <td width="15"><p align="left">&nbsp;7</p></td>
 +
            <td width="96"><p align="left">BBa_K1523023</p></td>
 +
            <td width="131"><p align="left">Lanzhou University </p></td>
 +
            <td width="121"><p align="left"><em>Pseudomonas fluorescens</em></p></td>
 +
            <td width="96" valign="top"><p align="left">Chromate    reduction</p></td>
 +
            <td width="51"><p align="left">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1</p></td>
 +
            <td width="130" valign="top"><p align="left">1.&nbsp; Strain used in cathode of MFC <br />
 +
              2.&nbsp; Other organic pollutant reduction in    cathode</p></td>
 +
          </tr>
 +
          <tr>
 +
            <td width="15"><p align="left">&nbsp;8</p></td>
 +
            <td width="96"><p align="left">BBa_K1523025</p></td>
 +
            <td width="131"><p align="left">Lanzhou University </p></td>
 +
            <td width="121"><p align="left"><em>Pseudomonas </em><em>putida</em></p></td>
 +
            <td width="96" valign="top"><p align="left">Chromate    reduction</p></td>
 +
            <td width="51"><p align="left">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1</p></td>
 +
            <td width="130" valign="top"><p align="left">1.&nbsp; Strain used in anode of MFC 2.&nbsp; Chromate reduction in anode</p></td>
 +
          </tr>
 +
        </table>
 +
        <p>&nbsp;</p>
 +
        <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We have taken safety courses before so the entire  group members know how to protect ourselves in experiments. <br />
 +
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;As for environment, some of the masses hesitate it  may cause genetic pollution to the environment, but it should be noticed that  the gene modified E.coli we construct cannot express our target gene unless it  is stimulated by the pollutant PNP.</p>
 +
<p>&nbsp;</p>
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        <p>&nbsp;</p>
 +
        <p>&nbsp;</p>
 +
        <p>&nbsp;</p>
 +
        <p>&nbsp;</p>
 +
        <p>&nbsp;</p>
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        <p>&nbsp;</p>
 +
        <p>&nbsp;</p>
 +
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 +
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Revision as of 21:26, 16 October 2014

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> LZU-China 2014

 
 
          SAFETY

 

 

          

          

 

Species

Strain no/name

Risk Group

Disease risk to humans? If so, which disease

 1

E. coliK 12

DH5 alpha

1

Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. However, E. coli K-12 is not considered a human or animal pathogen nor is it toxicogenic. Any concerns for E. coli K-12 in terms of health considerations are mitigated by its poor ability to colonize the colon and establish infections.

 2

Shewenella Onedensis

MR1

1

No

 

 

 

Part number

Where did us get the physical DNA for this part

What species does this part originally come from?

 

Natural function of part

What is the Risk Group of the species?

How will you use it?

 1

pSB1C3

From Li Xiangkai's Lab, Lanzhou University

Escherichia coli K-12

Vector for Escherichia coli

     1
  1. Vector for E.coli to express BBA_K1523101
  2. Submission vector for all parts

 2

BBA_K1523101

From distributed kit of iGEM 2013
BBA­_K381001

Escherichia coli K-12

Detector of Nitrate

     1

1.  Strain used in anode of MFC 2.  Detector of PNP
3.  Riboflavin producing

 3

pET-28(a) +

From Li Xiangkai's Lab, Lanzhou University

Vector for Escherichia coli

Vector for Escherichia coli

     1

Vector for E.coli to express BBA_K1523101

 4

BBa_K1523008

Lanzhou University

Escherichia coli

Chromate reduction

     1

1.  Strain used in anode of MFC 2.  Chromate reduction in anode

 5

BBa_K1523021

Lanzhou University

Pseudomonas fluorescens

Chromate reduction

     1

1.  Strain used in cathode of MFC
2.  Other organic pollutant reduction in cathode

 6

BBa_K1523022

Lanzhou University

Pseudomonas fluorescens

Chromate reduction

     1

1.  Strain used in cathode of MFC
2.  Other organic pollutant reduction in cathode

 7

BBa_K1523023

Lanzhou University

Pseudomonas fluorescens

Chromate reduction

     1

1.  Strain used in cathode of MFC
2.  Other organic pollutant reduction in cathode

 8

BBa_K1523025

Lanzhou University

Pseudomonas putida

Chromate reduction

     1

1.  Strain used in anode of MFC 2.  Chromate reduction in anode

 

        We have taken safety courses before so the entire group members know how to protect ourselves in experiments.
        As for environment, some of the masses hesitate it may cause genetic pollution to the environment, but it should be noticed that the gene modified E.coli we construct cannot express our target gene unless it is stimulated by the pollutant PNP.

 

 

 

 

 

 

 

 


  

 

 

 
 
 

Retrieved from "http://2014.igem.org/Team:LZU-China/Safety"