Team:LZU-China/Parts

From 2014.igem.org

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       <td width="90%"><p>&nbsp;</p>
         <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
         <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
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             <td width="34"><p align="center">1</p></td>
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             <td width="72">No</td>
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             <td width="134"><p align="center">BBa_K1523021</p></td>
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            <td width="139">Part No.</td>
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             <td width="72"><p align="center">pSB1C3</p></td>
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             <td width="96">NCBI No.</td>
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             <td width="52"><p align="center">C</p></td>
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            <td width="72">Resistance</td>
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             <td width="266"><p align="center"><em>nahR</em> coding    sequence,encoding a <em>LysR</em>-type transcriptional regulator </p></td>
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             <td width="72">Vector</td>
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             <td width="95"><p align="center"><em>903bp</em></p></td>
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            <td width="72">Gene Name</td>
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             <td width="244">Gene function</td>
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             <td width="72">Length</td>
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             <td width="333">Website (NCBI)</td>
           </tr>
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             <td width="34"><p align="center">2</p></td>
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             <td>1</td>
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             <td width="134"><p align="center">BBa_K1523022</p></td>
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            <td>BBa_K1523021</td>
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             <td width="72"><p align="center">pSB1C3</p></td>
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             <td>NC_004999.1</td>
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             <td width="52"><p align="center">C</p></td>
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            <td>Chl</td>
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             <td width="266"><p align="center"><em>nahE</em> coding sequence </p></td>
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             <td>pSB1C3</td>
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             <td width="95"><p align="center"><em>1005bp</em></p></td>
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             <td>nahR</td>
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             <td width="244">nahE&nbsp;coding sequence, <br />
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              encoding a LysR-type transcriptional regulator</td>
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            <td>903 bp</td>
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             <td><a href="http://www.ncbi.nlm.nih.gov/gene/1343208">http://www.ncbi.nlm.nih.gov/gene/1343208</a></td>
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             <td width="34"><p align="center">3</p></td>
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             <td>2</td>
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             <td width="134"><p align="center">BBa_K1523023</p></td>
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            <td>BBa_K1523022</td>
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             <td width="72"><p align="center">pSB1C3</p></td>
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             <td width="96">NC_007926.1<br />
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             <td width="52"><p align="center">C</p></td>
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              (37386..38381)</td>
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             <td width="266"><p align="center"><em>nahG</em> coding sequence </p></td>
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             <td>Chl</td>
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             <td width="95"><p align="center"><em>1305bp</em></p></td>
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            <td>pSB1C4</td>
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             <td>nahE</td>
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             <td>nahE&nbsp;coding sequence</td>
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            <td>1005 bp</td>
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             <td><a href="http://www.ncbi.nlm.nih.gov/gene/3974214">http://www.ncbi.nlm.nih.gov/gene/3974214</a></td>
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             <td width="34"><p align="center">4</p></td>
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             <td>3</td>
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             <td width="134"><p align="center">BBa_K1523025</p></td>
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            <td>BBa_K1523023</td>
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             <td width="72"><p align="center">pSB1C3</p></td>
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             <td width="96">NC_007926.1<br />
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             <td width="52"><p align="center">C</p></td>
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              (44492..45796)</td>
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             <td width="266"><p align="center"><em>chrR</em> coding sequence </p></td>
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             <td>Chl</td>
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             <td width="95"><p align="center"><em>566bp</em></p></td>
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            <td>pSB1C5</td>
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             <td>nahG</td>
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             <td>nahG&nbsp;coding sequence</td>
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            <td>1305 bp</td>
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             <td><a href="http://www.ncbi.nlm.nih.gov/gene/3974216">http://www.ncbi.nlm.nih.gov/gene/3974216</a></td>
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             <td width="34"><p align="center">5</p></td>
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             <td>4</td>
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             <td width="134"><p align="center">BBa_K1523101</p></td>
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            <td>BBa_K1523025</td>
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             <td width="72"><p align="center">pSB1C3</p></td>
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             <td>NC_017530.1</td>
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             <td width="52"><p align="center">C</p></td>
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            <td>Chl</td>
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             <td width="266"><p align="center"><em>PyeaR</em>-&gt;Riboflavin synthase </p></td>
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             <td>pSB1C6</td>
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             <td width="95"><p align="center"><em>3726bp</em></p></td>
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             <td>chrR</td>
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             <td>chrR&nbsp;coding sequence</td>
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            <td>666 bp</td>
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             <td><a href="http://www.ncbi.nlm.nih.gov/gene/12519943">http://www.ncbi.nlm.nih.gov/gene/12519943</a></td>
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             <td width="34"><p align="center">6</p></td>
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             <td>5</td>
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             <td width="134"><p align="center">BBa_K1523008</p></td>
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            <td>BBa_K1523101</td>
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             <td width="72"><p align="center">pSB1C3</p></td>
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            <td>N/A</td>
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             <td width="52"><p align="center">C</p></td>
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             <td>Chl</td>
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             <td width="266"><p align="center"><em>yieF</em> coding sequence </p></td>
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            <td>pSB1C7</td>
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             <td width="95"><p align="center"><em>560bp</em></p></td>
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            <td>PyeaR</td>
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             <td>PyeaR-&gt;Riboflavin synthase</td>
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            <td>3726 bp</td>
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            <td>N/A</td>
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          </tr>
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          <tr>
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             <td>6</td>
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            <td>BBa_K1523008</td>
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            <td>NC_007779.1</td>
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             <td>Chl</td>
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            <td>pSB1C8</td>
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            <td>Yief</td>
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            <td>yieF&nbsp;coding   sequence</td>
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            <td>560 bp</td>
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             <td><a href="http://www.ncbi.nlm.nih.gov/gene/12519943">http://www.ncbi.nlm.nih.gov/gene/12933177</a></td>
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  <div>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;GEL PICTURE OF SOME PARTS</div>
 
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Revision as of 03:33, 18 October 2014

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> LZU-China 2014

 
 
          SUMMARY OF THE PARTS

 

 

 

          

No Part No. NCBI No. Resistance Vector Gene Name Gene function Length Website (NCBI)
1 BBa_K1523021 NC_004999.1 Chl pSB1C3 nahR nahE coding sequence,
encoding a LysR-type transcriptional regulator
903 bp http://www.ncbi.nlm.nih.gov/gene/1343208
2 BBa_K1523022 NC_007926.1
(37386..38381)
Chl pSB1C4 nahE nahE coding sequence 1005 bp http://www.ncbi.nlm.nih.gov/gene/3974214
3 BBa_K1523023 NC_007926.1
(44492..45796)
Chl pSB1C5 nahG nahG coding sequence 1305 bp http://www.ncbi.nlm.nih.gov/gene/3974216
4 BBa_K1523025 NC_017530.1 Chl pSB1C6 chrR chrR coding sequence 666 bp http://www.ncbi.nlm.nih.gov/gene/12519943
5 BBa_K1523101 N/A Chl pSB1C7 PyeaR PyeaR->Riboflavin synthase 3726 bp N/A
6 BBa_K1523008 NC_007779.1 Chl pSB1C8 Yief yieF coding sequence 560 bp http://www.ncbi.nlm.nih.gov/gene/12933177

 

 

 


 
 

 

          GEL PICTURE OF SOME PARTS
 

 

 

 

 

Fig.2 The picture of K1523101. 1,3: pSB1C3 with K1523101 cut by EcoRI; 2,4: pSB1C3 with K11523101 cut by EcoRI and HindIII; 5: pSB1C3 with K1523101 not be cut.