Team:LZU-China/Parts

From 2014.igem.org

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:LZU-China/Parts&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:LZU-China"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:LZU-China/Team"style="color:#000000"> Team </a> </td>
 
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<a href="https://igem.org/Team.cgi?year=2014&team_name=LZU-China"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:LZU-China/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:LZU-China/Parts"style="color:#000000"> Parts</a></td>
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<tr><td > <h3> Parts Submitted to the Registry </h3></td>
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<td > <h3>What information do I need to start putting my parts on the Registry? </h3></td>
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An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will submit them to the <a href="http://partsregistry.org"> Registry of Standard Biological Parts</a>. The iGEM software provides an easy way to present the parts your team has created. The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox. 
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<strong>Note that if you want to document a part you need to document it on the <a href="http://partsregistry.org Registry"> Registry</a>, not on your team wiki.</strong> Future teams and other users and are much more likely to find parts on the Registry than on your team wiki.
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Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without a need to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
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<h3>When should you put parts into the Registry?</h3>
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As soon as possible! We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better recall you will have of all details surrounding your parts. Remember you don't need to send us the DNA to create an entry for a part on the Registry. However, you must send us the sample/DNA before the Jamboree. Only parts for which you have sent us samples/DNA are eligible for awards and medal requirements.
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.annCentered h3 {
 +
padding: 0px;
 +
display: block;
 +
margin-left: auto;
 +
margin-right: auto;
 +
text-align: center;
 +
}
 +
 
 +
.annItem ul, .annItem li {padding: 0px; margin: 0px;}
 +
.annItem li {list-style: none; margin: 5px}
 +
 
 +
@charset "utf-8";
 +
body,h1,h2,h3,h4,h5,h6,hr,p,blockquote,dl,dt,dd,ul,ol,li,pre,form,fieldset,legend,button,input,textarea,th,td,figure{
 +
margin:0;
 +
padding:0;
 +
font-size: 14px;
 +
text-align: left;
 +
}
 +
html{background-color:#e9ecec}
 +
table{table-layout:fixed;border-collapse:collapse}
 +
section,article,aside,header,footer,nav,dialog,figure{display:block}
 +
.clearfix:after{content:"\20";display:block;height:0;line-height:0;font-size:0;clear:both}
 +
body,button,input,select,textarea,td,th{color:#333;font:12px/1.5 tahoma,arial,\5b8b\4f53}
 +
h1,h2,h3,h4,h5,h6{font-size:100%}
 +
address,cite,dfn,em,var{font-style:normal}
 +
code,kbd,pre,samp{font-family:courier new,courier,monospace}
 +
small{font-size:12px}ul,ol{list-style:none}a{text-decoration:none;color:#06C}
 +
a:hover{text-decoration:underline;color:#06C}
 +
sup{vertical-align:text-top}
 +
sub{vertical-align:text-bottom}
 +
legend{color:#000}
 +
fieldset,img{border:0 none}
 +
button,input,select,textarea{font-size:100%}
 +
table{
 +
border-collapse:collapse;
 +
border-spacing:0;
 +
text-align: center;
 +
}
 +
input:focus{outline:none}
 +
.hd-main{height:60px;background:#fff;z-index:15;border-bottom:#b3b3b3 1px solid;box-shadow:0 0 5px #333}
 +
.f-icon{background-image:url('http://www.codefans.net/jscss/demoimg/201403//frame-icon.png');background-repeat:no-repeat}
 +
.hd-main{padding:0 5px;height:49px;min-width:800px;background:#252525;border-bottom:0}
 +
.hd-main .has-pulldown{cursor:pointer;position:relative;*z-index:10000}
 +
.hd-main .pulldown{position:absolute;cursor:default;display:none;top:30px;left:0}
 +
.hd-main .pulldown .arrow{*margin-bottom:-1px;_margin-bottom:0;height:0;width:0;font-size:0;line-height:0;border-width:7px;border-style:solid;border-color:transparent transparent rgba(255, 255, 255,0.8) transparent;_filter:chroma(color=tomato);_border-color:tomato tomato rgba(255, 255, 255,0.8) tomato;position:relative;display:block;left:20px;z-index:7}
 +
.hd-main .pulldown .content{background:rgba(255, 255, 255,0.8);color:#333;text-align:left;border-radius:3px;border:rgb(175,175,175) 1px solid;border-width:0 1px 1px 1px;box-shadow:0 2px 4px rgba(0,0,0,.2);position:relative;z-index:6}
 +
.hd-main .pulldown-canvas{position:absolute;top:0;left:0;width:100%;height:100%;opacity:0;filter:alpha(opacity=0)}
 +
.hd-main .logo{background-image:url('https://static.igem.org/mediawiki/2014/7/72/2014logomin.png');background-repeat:no-repeat;display:block;height:36px;width:175px;float:left;margin-left:10px;background-position:0 0;margin-top:5px;display:inline}
 +
.hd-main .navs{padding:0 0 3px 25px;height:45px;float:left}
 +
.hd-main .navs a,.hd-main .info a,.hd-main .navs a:visited,.hd-main .info a:visited,.hd-main .navs a:active,.hd-main .info a:active{color:#d8d8d8}
 +
.hd-main .navs a:hover,.hd-main .navs a:active{text-decoration:none;background-color:#0f0f0f}
 +
.hd-main .navs .def-nav,.hd-main .navs .pulldown-nav,.hd-main .navs .separate{display:block;float:left;height:48px;font:18px/30px "Microsoft YaHei","Microsoft JhengHei";color:#d8d8d8;text-align:center;width:125px;line-height:48px}
 +
.hd-main .navs .def-nav .app-url{left:185px;width:425px;top:48px}
 +
.hd-main .navs .def-nav .pulldown-canvas-url{width:575px;height:109px}
 +
.hd-main .navs .def-nav .app-url .arrow{left:140px}
 +
.hd-main .navs .app-url .content{padding:17px 0 0 22px;height:91px}
 +
.hd-main .navs .app-url .content a,.hd-main .navs .app-url .content a:hover{color:#333}
 +
.hd-main .navs .app-url .li{cursor:pointer;padding-top:8px;width:66px;height:66px;border-radius:3px;display:block;float:left;text-align:center}
 +
.hd-main .navs .app-url .li:hover{background-color:#e0e1e5}
 +
.hd-main .navs .app-url .li .icon{margin:0 auto;width:50px;height:50px;display:block;background:url('https://static.igem.org/mediawiki/2014/9/94/Icons50X50nb.png') no-repeat}
 +
.hd-main .navs .app-url .li:hover .icon{background-image:url('https://static.igem.org/mediawiki/2014/9/94/Icons50X50nb.png')}
 +
.hd-main .navs .app-url .tongxunlu .icon{background-position:-50px 0}
 +
.hd-main .navs .app-url .xiangce .icon{background-position:-100px 0}
 +
.hd-main .navs .app-url .note .icon{background-position:-150px 0}
 +
.hd-main .navs .app-url .duanxin .icon{background-position:-200px 0}
 +
.hd-main .navs .app-url .zhaohui .icon{background-position:-250px 0}
 +
.hd-main .navs .app-url .youxi .icon{background-position:-300px 0}
 +
.hd-main .navs .app-url .li .text{height:30px;display:block;font:10px/30px "Microsoft YaHei","Microsoft JhengHei"}
 +
.hd-main .navs .separate{margin:0;height:48px;width:2px;background:#2A2B2D}
 +
.hd-main .navs .pulldown-nav{position:relative}
 +
.hd-main .navs .pulldown-nav em{
 +
position:absolute;
 +
display:block;
 +
left:99px;
 +
top:18px;
 +
height:12px;
 +
width:12px;
 +
font-size:0;
 +
background-position:-89px -45px
 +
}
 +
.hd-main .navs .pulldown-nav:hover .f-icon,.hd-main .navs .pulldown-nav:active .f-icon{position:absolute;display:block;right:11px;top:18px;height:12px;width:12px;font-size:0;background-position:-89px -55px}
 +
.hd-main .navs .current,.hd-main .navs .current .pulldown-nav{font-weight:bold;color:#FFF;display:inline-block;width:125px;height:49px;background-color:#0f0f0f;text-align:center;line-height:48px}
 +
.hd-main .navs .current:hover{color:#ECECEC}
 +
.hd-main .navs .current .hover{background-color:#dedede;color:#333;}
 +
.hd-main .info,.hd-main .info ul{height:36px;float:right;text-align:right;margin-top:3px;white-space:nowrap}
 +
.hd-main .info a{color:#d8d8d8}
 +
.hd-main .info a:hover{color:#FFF;text-decoration:underline}
 +
.hd-main .info .info-i{padding:0 10px;margin:10px 0;display:inline-block;*display:inline;*zoom:1;height:16px;border-right:#393939 1px solid;color:#d8d8d8;font:12px/16px "SimSun"}
 +
.hd-main{padding:0 5px;height:49px;min-width:800px;background:#252525;border-bottom:0}
 +
.hd-main .info .has-pulldown{padding-right:28px}
 +
.hd-main .info .has-pulldown .pull-arrow{position:absolute;display:block;right:10px;top:1px;height:12px;width:12px;font-size:0;background-position:-89px -45px}
 +
.hd-main .info .no-separate{border:none}
 +
.hd-main .info .user-name{position:relative;padding-left:24px}
 +
.hd-main .info .user-name .name{display:inline-block;display:inline\0;white-space:nowrap;max-width:75px;overflow:hidden;text-overflow:ellipsis;vertical-align:bottom}
 +
.hd-main .info .user-name .identity{position:absolute;top:0;left:0;height:16px;width:16px;background-position:-89px -88px}
 +
.hd-main .info .user-name .is-vip{background-position:-89px -68px}
 +
.hd-main .info .user-info,.hd-main .info .more-info{top:9px;left:auto;right:-24px;width:170px} /*User栏目宽度*/
 +
.hd-main .info .notice-nu{display:inline-block;padding-left:4px;background-position:-86px -109px;height:14px;-webkit-text-size-adjust:none;font-size:10px;font-style:normal}
 +
.hd-main .info .notice-nu .f-icon{display:inline-block;padding-right:4px;background-position:right -109px;color:#FFF}  /*此处原为#FFF*/
 +
.hd-main .info .user-info .arrow{left:70px;*left:-32px}
 +
.hd-main .info .user-info .content,.hd-main .info .more-info .content{padding:10px 0;height:180px}
 +
.hd-main .info .more{padding:0;display:inline;line-height:inherit;background:none}
 +
.hd-main .info .more-info .content{height:150px}
 +
.hd-main .info .user-info .content a,.hd-main .info .more-info .content a{display:block;padding-left:33px;height:30px;line-height:30px;color:#888}
 +
.hd-main .info .user-info .content a:hover,.hd-main .info .more-info .content a:hover{color:#333;text-decoration:none;background:#e0e1e5}
 +
.hd-main .info .user-info .li,.hd-main .info .more-info .li{display:block;padding:2px 0;height:30px}
 +
.hd-main .info .user-info .separate-li,.hd-main .info .more-info .separate-li{display:block;margin:3px 11px;border-top:#EBEBEC 1px solid;border-bottom:#FFF 1px solid}
 +
.hd-main .info .more-info{right:0}
 +
.hd-main .info .more-info .arrow{left:94px;*left:-8px}
 +
#apDiv1 {
 +
position:absolute;
 +
left:3px;
 +
top:4px;
 +
width:100%;
 +
height:900px;
 +
z-index:0;
 +
background-color: #66CCFF;
 +
}
 +
/*<![CDATA[*/
 +
body {
 +
margin:0;
 +
padding:0 10px 0 10px;
 +
border:0;
 +
height:100%;
 +
overflow:auto;
 +
background-color: #FFF;
 +
}
 +
#menu {right:0; top:0; width:100%; position:fixed; padding:10px; text-align:center; font-weight:bold; background:rgba(0,83,163,0);}
 +
* html #menu {position:absolute; right:16px; top:0;}/*only for ie*/
 +
html {overflow:auto !important; overflow:hidden;}
 +
 
 +
#apDiv2 {
 +
position:absolute;
 +
left:0;
 +
top:50px;
 +
width:100%;
 +
height:265px;
 +
z-index:0;
 +
background-color: #187BDB;
 +
}
 +
#apDiv3 {
 +
position:absolute;
 +
left:0px;
 +
top:328px;
 +
width:100%;
 +
height:704px;
 +
z-index:0;
 +
}
 +
#apDiv3 table tr td p {
 +
font-size: 17px;
 +
text-align: left;
 +
line-height: 35px;
 +
}
 +
#apDiv4 {
 +
position:absolute;
 +
left:0;
 +
top:9704px;
 +
width:100%;
 +
height:56px;
 +
z-index:0;
 +
}
 +
#apDiv5 {
 +
position:absolute;
 +
left:0;
 +
top:1888px;
 +
width:100%;
 +
height:25;
 +
z-index:1;
 +
background-color: #5FC1C5;
 +
}
 +
#apDiv3 div {
 +
font-size: 46px;
 +
text-align: center;
 +
}
 +
#apDiv4 table tr td div {
 +
font-size: 45px;
 +
color: #000;
 +
text-align: left;
 +
}
 +
#apDiv4 table tr td table tr td {
 +
font-size: 16px;
 +
line-height: 30px;
 +
}
 +
body,td,th {
 +
color: #000;
 +
}
 +
#apDiv6 {
 +
position:absolute;
 +
left:575px;
 +
top:1777px;
 +
width:220px;
 +
height:221px;
 +
z-index:2;
 +
}
 +
#apDiv2 table tr td p {
 +
font-size: 45px;
 +
text-align: center;
 +
color: #FFF;
 +
font-weight: bold;
 +
}
 +
#apDiv7 {
 +
position:absolute;
 +
left:50%;
 +
top:226px;
 +
width:257px;
 +
height:151px;
 +
z-index:auto;
 +
}
 +
#apDiv3 div {
 +
font-size: 45px;
 +
text-align: left;
 +
}
 +
#apDiv8 {
 +
position:absolute;
 +
left:0;
 +
top:680px;
 +
width:100%;
 +
height:470px;
 +
z-index:0;
 +
}
 +
#apDiv4 table tr td table tr td p {
 +
font-size: 18px;
 +
line-height: 32px;
 +
}
 +
#apDiv8 table tr td table tr td {
 +
font-size: 45px;
 +
}
 +
#apDiv8 table tr td table tr td p {
 +
font-size: 18px;
 +
line-height: 31px;
 +
}
 +
#apDiv9 {
 +
position:absolute;
 +
left:0px;
 +
top:6251px;
 +
width:100%;
 +
height:55px;
 +
z-index:4;
 +
}
 +
#apDiv9 table tr td table tr td table tr td {
 +
font-size: 35px;
 +
}
 +
#apDiv9 table tr td table tr td table tr td p {
 +
font-size: 18px;
 +
line-height: 31px;
 +
}
 +
#apDiv4 table tr td table tr td div div {
 +
font-size: 15px;
 +
}
 +
#apDiv4 table tr td table tr td table tr td {
 +
font-size: 15px;
 +
}
 +
#apDiv4 table tr td table tr td table tr td {
 +
font-size: 15px;
 +
}
 +
#apDiv8 table tr td table tr td table tr td {
 +
font-size: 18px;
 +
}
 +
#apDiv10 {
 +
position:absolute;
 +
left:0;
 +
top:6956px;
 +
width:1004px;
 +
height:539px;
 +
z-index:5;
 +
}
 +
#apDiv9 table tr td table tr td table tr td table tr td {
 +
font-size: 18px;
 +
}
 +
#apDiv11 {
 +
position:absolute;
 +
left:0;
 +
top:7440px;
 +
width:100%;
 +
height:140px;
 +
z-index:5;
 +
}
 +
#apDiv11 table tr td table tr td {
 +
font-size: 35px;
 +
}
 +
#apDiv11 table tr td table tr td p {
 +
font-size: 18px;
 +
line-height: 32px;
 +
}
 +
#apDiv11 table tr td table tr td table tr td {
 +
font-size: 18px;
 +
}
 +
#apDiv3 table tr td {
 +
font-size: 18px;
 +
line-height: 32px;
 +
}
 +
#apDiv3 table tr td table tr td p {
 +
font-size: 17px;
 +
text-align: center;
 +
}
 +
#apDiv3 table tr td p {
 +
text-align: center;
 +
font-size: 18px;
 +
}
 +
</style>
 +
</head>
-
<p>
+
<body id="" class="">
-
The information needed to initially create a part on the Registry is:
+
<div id="apDiv2">
-
</p>
+
  <table width="100%" border="0" >
-
<ol>
+
    <tr>
 +
      <td width="1201" height="265" rowspan="2"><p><img src="https://static.igem.org/mediawiki/2014/0/0a/4.parts.top.jpg" width="1366" height="270" /></p></td>
 +
      <td width="100%" height="199" bgcolor="#187ADB">&nbsp;</td>
 +
    </tr>
 +
    <tr>
 +
      <td height="70">&nbsp;</td>
 +
    </tr>
 +
  </table>
 +
</div>
 +
<div id="apDiv3">
 +
  <div>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;FUTURE DIRECTIONS</div>
 +
  <p>&nbsp;</p>
 +
  <table width="100%" border="0">
 +
    <tr>
 +
      <td width="22%" height="408">&nbsp;</td>
 +
      <td width="56%"><p>&nbsp;</p>
 +
        <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
 +
        <table width="653" border="0" align="center" cellpadding="0" cellspacing="0">
 +
          <tr>
 +
            <td width="34"><p align="center">1</p></td>
 +
            <td width="134"><p align="center">BBa_K1523021</p></td>
 +
            <td width="72"><p align="center">pSB1C3</p></td>
 +
            <td width="52"><p align="center">C</p></td>
 +
            <td width="266"><p align="center"><em>nahR</em> coding    sequence,encoding a <em>LysR</em>-type transcriptional regulator </p></td>
 +
            <td width="95"><p align="center"><em>903bp</em></p></td>
 +
          </tr>
 +
          <tr>
 +
            <td width="34"><p align="center">2</p></td>
 +
            <td width="134"><p align="center">BBa_K1523022</p></td>
 +
            <td width="72"><p align="center">pSB1C3</p></td>
 +
            <td width="52"><p align="center">C</p></td>
 +
            <td width="266"><p align="center"><em>nahE</em> coding sequence </p></td>
 +
            <td width="95"><p align="center"><em>1005bp</em></p></td>
 +
          </tr>
 +
          <tr>
 +
            <td width="34"><p align="center">3</p></td>
 +
            <td width="134"><p align="center">BBa_K1523023</p></td>
 +
            <td width="72"><p align="center">pSB1C3</p></td>
 +
            <td width="52"><p align="center">C</p></td>
 +
            <td width="266"><p align="center"><em>nahG</em> coding sequence </p></td>
 +
            <td width="95"><p align="center"><em>1305bp</em></p></td>
 +
          </tr>
 +
          <tr>
 +
            <td width="34"><p align="center">4</p></td>
 +
            <td width="134"><p align="center">BBa_K1523025</p></td>
 +
            <td width="72"><p align="center">pSB1C3</p></td>
 +
            <td width="52"><p align="center">C</p></td>
 +
            <td width="266"><p align="center"><em>chrR</em> coding sequence </p></td>
 +
            <td width="95"><p align="center"><em>566bp</em></p></td>
 +
          </tr>
 +
          <tr>
 +
            <td width="34"><p align="center">5</p></td>
 +
            <td width="134"><p align="center">BBa_K1523101</p></td>
 +
            <td width="72"><p align="center">pSB1C3</p></td>
 +
            <td width="52"><p align="center">C</p></td>
 +
            <td width="266"><p align="center"><em>PyeaR</em>-&gt;Riboflavin synthase </p></td>
 +
            <td width="95"><p align="center"><em>3726bp</em></p></td>
 +
          </tr>
 +
          <tr>
 +
            <td width="34"><p align="center">6</p></td>
 +
            <td width="134"><p align="center">BBa_K1523008</p></td>
 +
            <td width="72"><p align="center">pSB1C3</p></td>
 +
            <td width="52"><p align="center">C</p></td>
 +
            <td width="266"><p align="center"><em>yieF</em> coding sequence </p></td>
 +
            <td width="95"><p align="center"><em>560bp</em></p></td>
 +
          </tr>
 +
        </table>
 +
<p>&nbsp;</p>
 +
<p>&nbsp;</p>
 +
        <p><br />
 +
      </p></td>
 +
      <td width="22%">&nbsp;</td>
 +
    </tr>
 +
  </table>
 +
  <div>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;GEL PICTURE OF SOME PARTS</div>
 +
  <p>&nbsp;</p>
 +
  <table width="100%" border="0">
 +
    <tr>
 +
      <td width="22%" height="408">&nbsp;</td>
 +
      <td width="56%"><p>&nbsp;</p>
 +
        <p><img src="https://static.igem.org/mediawiki/2014/c/c0/Partpic1.png" width="560" height="378" /></p>
 +
        <p>&nbsp;</p>
 +
        <p><img src="https://static.igem.org/mediawiki/2014/e/e0/Partpic2.png" width="301" height="251" /></p>
 +
        <p>Fig.2 The picture of K1523101. 1,3: pSB1C3 with K1523101 cut by EcoRI; 2,4: pSB1C3 with K11523101 cut by EcoRI and HindIII; 5: pSB1C3 with K1523101 not be cut.</p>
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<li>Part Name</li>
 
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<li>Part type</li>
 
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<li>Creator</li>
 
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<li>Sequence</li>
 
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<li>Short Description (60 characters on what the DNA does)</li>
 
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<li>Long Description (Longer description of what the DNA does)</li>
 
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<li>Design considerations</li>
 
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</ol>
 
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<p>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. Check out part <a href="http://parts.igem.org/Part:BBa_K404003">BBa_K404003</a> for an excellent example of a highly characterized part.
 
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You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry"> Add a Part to the Registry</a> link.
 
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<tr><td colspan="3" > <h3> Parts Table</h3></td></tr>
 
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<tr><td width="45%" colspan="3"  valign="top">
 
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Any parts your team has created will appear in this table below:</td></tr>
 
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</table>
 
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</html>
 
-
<groupparts>iGEM013 LZU-China</groupparts>
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Revision as of 02:29, 17 October 2014

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> LZU-China 2014

 
 
          FUTURE DIRECTIONS

 

 

 

          

1

BBa_K1523021

pSB1C3

C

nahR coding sequence,encoding a LysR-type transcriptional regulator

903bp

2

BBa_K1523022

pSB1C3

C

nahE coding sequence

1005bp

3

BBa_K1523023

pSB1C3

C

nahG coding sequence

1305bp

4

BBa_K1523025

pSB1C3

C

chrR coding sequence

566bp

5

BBa_K1523101

pSB1C3

C

PyeaR->Riboflavin synthase

3726bp

6

BBa_K1523008

pSB1C3

C

yieF coding sequence

560bp

 

 


 
          GEL PICTURE OF SOME PARTS

 

 

 

 

Fig.2 The picture of K1523101. 1,3: pSB1C3 with K1523101 cut by EcoRI; 2,4: pSB1C3 with K11523101 cut by EcoRI and HindIII; 5: pSB1C3 with K1523101 not be cut.