Team:LZU-China/Notebook

From 2014.igem.org

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:LZU-China/Notebook&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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padding: 0px;
-
  </v:formulas>
+
margin: 0px;
-
  <v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/>
+
}
-
  <o:lock v:ext="edit" aspectratio="t"/>
+
 
-
  </v:shapetype><v:shape id="图片_x0020_3" o:spid="_x0000_i1026" type="#_x0000_t75"
+
.newsItem img {
-
  style='width:515.25pt;height:151.5pt;visibility:visible;mso-wrap-style:square'>
+
float: right;
-
  <v:imagedata src="http://i.minus.com/ievX6GA6Qa260.jpg" o:title=""/>
+
clear: right;
-
  </v:shape><![endif]-->
+
width: 80px;
-
        <![if !vml]>
+
padding-left: 15px;
-
        <img
+
display: inline-block;
-
  src="http://i.minus.com/ievX6GA6Qa260.jpg" alt="logo" width="687" height="202" v:shapes="图片_x0020_3" />
+
}
-
        <![endif]>
+
 
-
      </span></p></td>
+
.newsItem .newsDate {
 +
font-style: italic;
 +
font-size: 14px;
 +
display: inline-block;
 +
color: #999;
 +
float: right;
 +
padding: 0px 0px 10px 15px;
 +
}
 +
 
 +
.newsItem p, .newsItem ul, .newsItem li {
 +
padding: 0px;
 +
margin-left: 20px;
 +
font-size: 12px;
 +
line-height: 1.2;
 +
}
 +
 
 +
.newsItem p {margin-top: 10px;}
 +
 
 +
.annItem {
 +
border: 2px solid #49b677;
 +
display: block;
 +
padding: 0px 15px 10px 10px;
 +
margin-bottom: 18px;
 +
position: relative;
 +
height: 160px;
 +
vertical-align: middle;
 +
}
 +
 
 +
.annItem .annCentered {
 +
display: table-cell;
 +
vertical-align: middle;
 +
height: 160px;
 +
}
 +
 
 +
.annCentered h3 {
 +
padding: 0px;
 +
display: block;
 +
margin-left: auto;
 +
margin-right: auto;
 +
text-align: center;
 +
}
 +
 
 +
.annItem ul, .annItem li {padding: 0px; margin: 0px;}
 +
.annItem li {list-style: none; margin: 5px}
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@charset "utf-8";
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body,h1,h2,h3,h4,h5,h6,hr,p,blockquote,dl,dt,dd,ul,ol,li,pre,form,fieldset,legend,button,input,textarea,th,td,figure{
 +
margin:0;
 +
padding:0;
 +
font-size: 14px;
 +
text-align: left;
 +
}
 +
html{background-color:#e9ecec}
 +
table{table-layout:fixed;border-collapse:collapse}
 +
section,article,aside,header,footer,nav,dialog,figure{display:block}
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.clearfix:after{content:"\20";display:block;height:0;line-height:0;font-size:0;clear:both}
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body,button,input,select,textarea,td,th{color:#333;font:12px/1.5 tahoma,arial,\5b8b\4f53}
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h1,h2,h3,h4,h5,h6{font-size:100%}
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address,cite,dfn,em,var{font-style:normal}
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code,kbd,pre,samp{font-family:courier new,courier,monospace}
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small{font-size:12px}ul,ol{list-style:none}a{text-decoration:none;color:#06C}
 +
a:hover{text-decoration:underline;color:#06C}
 +
sup{vertical-align:text-top}
 +
sub{vertical-align:text-bottom}
 +
legend{color:#000}
 +
fieldset,img{border:0 none}
 +
button,input,select,textarea{font-size:100%}
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table{
 +
border-collapse:collapse;
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border-spacing:0;
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text-align: center;
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}
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input:focus{outline:none}
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.hd-main{height:60px;background:#fff;z-index:15;border-bottom:#b3b3b3 1px solid;box-shadow:0 0 5px #333}
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.f-icon{background-image:url('http://www.codefans.net/jscss/demoimg/201403//frame-icon.png');background-repeat:no-repeat}
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.hd-main{padding:0 5px;height:49px;min-width:800px;background:#252525;border-bottom:0}
 +
.hd-main .has-pulldown{cursor:pointer;position:relative;*z-index:10000}
 +
.hd-main .pulldown{position:absolute;cursor:default;display:none;top:30px;left:0}
 +
.hd-main .pulldown .arrow{*margin-bottom:-1px;_margin-bottom:0;height:0;width:0;font-size:0;line-height:0;border-width:7px;border-style:solid;border-color:transparent transparent rgba(255, 255, 255,0.8) transparent;_filter:chroma(color=tomato);_border-color:tomato tomato rgba(255, 255, 255,0.8) tomato;position:relative;display:block;left:20px;z-index:7}
 +
.hd-main .pulldown .content{background:rgba(255, 255, 255,0.8);color:#333;text-align:left;border-radius:3px;border:rgb(175,175,175) 1px solid;border-width:0 1px 1px 1px;box-shadow:0 2px 4px rgba(0,0,0,.2);position:relative;z-index:6}
 +
.hd-main .pulldown-canvas{position:absolute;top:0;left:0;width:100%;height:100%;opacity:0;filter:alpha(opacity=0)}
 +
.hd-main .logo{background-image:url('https://static.igem.org/mediawiki/2014/7/72/2014logomin.png');background-repeat:no-repeat;display:block;height:36px;width:175px;float:left;margin-left:10px;background-position:0 0;margin-top:5px;display:inline}
 +
.hd-main .navs{padding:0 0 3px 25px;height:45px;float:left}
 +
.hd-main .navs a,.hd-main .info a,.hd-main .navs a:visited,.hd-main .info a:visited,.hd-main .navs a:active,.hd-main .info a:active{color:#d8d8d8}
 +
.hd-main .navs a:hover,.hd-main .navs a:active{text-decoration:none;background-color:#0f0f0f}
 +
.hd-main .navs .def-nav,.hd-main .navs .pulldown-nav,.hd-main .navs .separate{display:block;float:left;height:48px;font:18px/30px "Microsoft YaHei","Microsoft JhengHei";color:#d8d8d8;text-align:center;width:125px;line-height:48px}
 +
.hd-main .navs .def-nav .app-url{left:185px;width:425px;top:48px}
 +
.hd-main .navs .def-nav .pulldown-canvas-url{width:575px;height:109px}
 +
.hd-main .navs .def-nav .app-url .arrow{left:140px}
 +
.hd-main .navs .app-url .content{padding:17px 0 0 22px;height:91px}
 +
.hd-main .navs .app-url .content a,.hd-main .navs .app-url .content a:hover{color:#333}
 +
.hd-main .navs .app-url .li{cursor:pointer;padding-top:8px;width:66px;height:66px;border-radius:3px;display:block;float:left;text-align:center}
 +
.hd-main .navs .app-url .li:hover{background-color:#e0e1e5}
 +
.hd-main .navs .app-url .li .icon{margin:0 auto;width:50px;height:50px;display:block;background:url('https://static.igem.org/mediawiki/2014/9/94/Icons50X50nb.png') no-repeat}
 +
.hd-main .navs .app-url .li:hover .icon{background-image:url('https://static.igem.org/mediawiki/2014/9/94/Icons50X50nb.png')}
 +
.hd-main .navs .app-url .tongxunlu .icon{background-position:-50px 0}
 +
.hd-main .navs .app-url .xiangce .icon{background-position:-100px 0}
 +
.hd-main .navs .app-url .note .icon{background-position:-150px 0}
 +
.hd-main .navs .app-url .duanxin .icon{background-position:-200px 0}
 +
.hd-main .navs .app-url .zhaohui .icon{background-position:-250px 0}
 +
.hd-main .navs .app-url .youxi .icon{background-position:-300px 0}
 +
.hd-main .navs .app-url .li .text{height:30px;display:block;font:10px/30px "Microsoft YaHei","Microsoft JhengHei"}
 +
.hd-main .navs .separate{margin:0;height:48px;width:2px;background:#2A2B2D}
 +
.hd-main .navs .pulldown-nav{position:relative}
 +
.hd-main .navs .pulldown-nav em{
 +
position:absolute;
 +
display:block;
 +
left:99px;
 +
top:18px;
 +
height:12px;
 +
width:12px;
 +
font-size:0;
 +
background-position:-89px -45px
 +
}
 +
.hd-main .navs .pulldown-nav:hover .f-icon,.hd-main .navs .pulldown-nav:active .f-icon{position:absolute;display:block;right:11px;top:18px;height:12px;width:12px;font-size:0;background-position:-89px -55px}
 +
.hd-main .navs .current,.hd-main .navs .current .pulldown-nav{font-weight:bold;color:#FFF;display:inline-block;width:125px;height:49px;background-color:#0f0f0f;text-align:center;line-height:48px}
 +
.hd-main .navs .current:hover{color:#ECECEC}
 +
.hd-main .navs .current .hover{background-color:#dedede;color:#333;}
 +
.hd-main .info,.hd-main .info ul{height:36px;float:right;text-align:right;margin-top:3px;white-space:nowrap}
 +
.hd-main .info a{color:#d8d8d8}
 +
.hd-main .info a:hover{color:#FFF;text-decoration:underline}
 +
.hd-main .info .info-i{padding:0 10px;margin:10px 0;display:inline-block;*display:inline;*zoom:1;height:16px;border-right:#393939 1px solid;color:#d8d8d8;font:12px/16px "SimSun"}
 +
.hd-main{padding:0 5px;height:49px;min-width:800px;background:#252525;border-bottom:0}
 +
.hd-main .info .has-pulldown{padding-right:28px}
 +
.hd-main .info .has-pulldown .pull-arrow{position:absolute;display:block;right:10px;top:1px;height:12px;width:12px;font-size:0;background-position:-89px -45px}
 +
.hd-main .info .no-separate{border:none}
 +
.hd-main .info .user-name{position:relative;padding-left:24px}
 +
.hd-main .info .user-name .name{display:inline-block;display:inline\0;white-space:nowrap;max-width:75px;overflow:hidden;text-overflow:ellipsis;vertical-align:bottom}
 +
.hd-main .info .user-name .identity{position:absolute;top:0;left:0;height:16px;width:16px;background-position:-89px -88px}
 +
.hd-main .info .user-name .is-vip{background-position:-89px -68px}
 +
.hd-main .info .user-info,.hd-main .info .more-info{top:9px;left:auto;right:-24px;width:170px} /*User栏目宽度*/
 +
.hd-main .info .notice-nu{display:inline-block;padding-left:4px;background-position:-86px -109px;height:14px;-webkit-text-size-adjust:none;font-size:10px;font-style:normal}
 +
.hd-main .info .notice-nu .f-icon{display:inline-block;padding-right:4px;background-position:right -109px;color:#FFF}  /*此处原为#FFF*/
 +
.hd-main .info .user-info .arrow{left:70px;*left:-32px}
 +
.hd-main .info .user-info .content,.hd-main .info .more-info .content{padding:10px 0;height:180px}
 +
.hd-main .info .more{padding:0;display:inline;line-height:inherit;background:none}
 +
.hd-main .info .more-info .content{height:150px}
 +
.hd-main .info .user-info .content a,.hd-main .info .more-info .content a{display:block;padding-left:33px;height:30px;line-height:30px;color:#888}
 +
.hd-main .info .user-info .content a:hover,.hd-main .info .more-info .content a:hover{color:#333;text-decoration:none;background:#e0e1e5}
 +
.hd-main .info .user-info .li,.hd-main .info .more-info .li{display:block;padding:2px 0;height:30px}
 +
.hd-main .info .user-info .separate-li,.hd-main .info .more-info .separate-li{display:block;margin:3px 11px;border-top:#EBEBEC 1px solid;border-bottom:#FFF 1px solid}
 +
.hd-main .info .more-info{right:0}
 +
.hd-main .info .more-info .arrow{left:94px;*left:-8px}
 +
#apDiv1 {
 +
position:absolute;
 +
left:3px;
 +
top:4px;
 +
width:100%;
 +
height:900px;
 +
z-index:0;
 +
background-color: #66CCFF;
 +
}
 +
/*<![CDATA[*/
 +
body {
 +
margin:0;
 +
padding:0 10px 0 10px;
 +
border:0;
 +
height:100%;
 +
overflow:auto;
 +
background-color: #FFF;
 +
}
 +
#menu {right:0; top:0; width:100%; position:fixed; padding:10px; text-align:center; font-weight:bold; background:rgba(0,83,163,0);}
 +
* html #menu {position:absolute; right:16px; top:0;}/*only for ie*/
 +
html {overflow:auto !important; overflow:hidden;}
 +
 
 +
#apDiv2 {
 +
position:absolute;
 +
left:0;
 +
top:50px;
 +
width:100%;
 +
height:265px;
 +
z-index:0;
 +
background-color: #187BDB;
 +
}
 +
#apDiv3 {
 +
position:absolute;
 +
left:0px;
 +
top:328px;
 +
width:100%;
 +
height:150px;
 +
z-index:0;
 +
}
 +
#apDiv3 table tr td p {
 +
font-size: 15px;
 +
text-align: left;
 +
}
 +
#apDiv4 {
 +
position:absolute;
 +
left:0;
 +
top:9704px;
 +
width:100%;
 +
height:56px;
 +
z-index:0;
 +
}
 +
#apDiv5 {
 +
position:absolute;
 +
left:0;
 +
top:6288px;
 +
width:100%;
 +
height:25;
 +
z-index:1;
 +
background-color: #5FC1C5;
 +
}
 +
#apDiv3 div {
 +
font-size: 46px;
 +
text-align: center;
 +
}
 +
#apDiv4 table tr td div {
 +
font-size: 45px;
 +
color: #000;
 +
text-align: left;
 +
}
 +
#apDiv4 table tr td table tr td {
 +
font-size: 16px;
 +
line-height: 30px;
 +
}
 +
body,td,th {
 +
color: #000;
 +
}
 +
#apDiv6 {
 +
position:absolute;
 +
left:575px;
 +
top:6177px;
 +
width:220px;
 +
height:221px;
 +
z-index:2;
 +
}
 +
#apDiv2 table tr td p {
 +
font-size: 45px;
 +
text-align: center;
 +
color: #FFF;
 +
font-weight: bold;
 +
}
 +
#apDiv7 {
 +
position:absolute;
 +
left:50%;
 +
top:226px;
 +
width:257px;
 +
height:151px;
 +
z-index:auto;
 +
}
 +
#apDiv3 div {
 +
font-size: 14px;
 +
}
 +
#apDiv8 {
 +
position:absolute;
 +
left:0;
 +
top:680px;
 +
width:100%;
 +
height:470px;
 +
z-index:3;
 +
}
 +
#apDiv4 table tr td table tr td p {
 +
font-size: 18px;
 +
line-height: 32px;
 +
}
 +
#apDiv8 table tr td table tr td {
 +
font-size: 45px;
 +
}
 +
#apDiv8 table tr td table tr td p {
 +
font-size: 18px;
 +
line-height: 31px;
 +
}
 +
#apDiv9 {
 +
position:absolute;
 +
left:0px;
 +
top:6251px;
 +
width:100%;
 +
height:55px;
 +
z-index:4;
 +
}
 +
#apDiv9 table tr td table tr td table tr td {
 +
font-size: 35px;
 +
}
 +
#apDiv9 table tr td table tr td table tr td p {
 +
font-size: 18px;
 +
line-height: 31px;
 +
}
 +
#apDiv4 table tr td table tr td div div {
 +
font-size: 15px;
 +
}
 +
#apDiv4 table tr td table tr td table tr td {
 +
font-size: 15px;
 +
}
 +
#apDiv4 table tr td table tr td table tr td {
 +
font-size: 15px;
 +
}
 +
#apDiv8 table tr td table tr td table tr td {
 +
font-size: 18px;
 +
}
 +
#apDiv10 {
 +
position:absolute;
 +
left:0;
 +
top:6956px;
 +
width:1004px;
 +
height:539px;
 +
z-index:5;
 +
}
 +
#apDiv9 table tr td table tr td table tr td table tr td {
 +
font-size: 18px;
 +
}
 +
#apDiv11 {
 +
position:absolute;
 +
left:0;
 +
top:7440px;
 +
width:100%;
 +
height:140px;
 +
z-index:5;
 +
}
 +
#apDiv11 table tr td table tr td {
 +
font-size: 35px;
 +
}
 +
#apDiv11 table tr td table tr td p {
 +
font-size: 18px;
 +
line-height: 32px;
 +
}
 +
#apDiv11 table tr td table tr td table tr td {
 +
font-size: 18px;
 +
}
 +
#apDiv3 table tr td {
 +
font-size: 18px;
 +
line-height: 32px;
 +
}
 +
</style>
 +
</head>
 +
 
 +
<body id="" class="">
 +
<div id="apDiv2">
 +
  <table width="100%" border="0" >
 +
    <tr>
 +
      <td width="1201" height="265" rowspan="2"><p><img src="https://static.igem.org/mediawiki/2014/d/de/6members.top.jpg" width="1366" height="270" /></p></td>
 +
      <td width="100%" height="199" bgcolor="#187ADB">&nbsp;</td>
     </tr>
     </tr>
-
     <tr style='mso-yfti-irow:1'>
+
     <tr>
-
       <td width="136" style='width:101.75pt;background:#FFC000;padding:.75pt .75pt .75pt .75pt'><p class="MsoNormal" align="center" style='text-align:center'><span
+
       <td height="70">&nbsp;</td>
-
  class="MsoHyperlink"><b style='mso-bidi-font-weight:normal'><span lang="EN-US" xml:lang="EN-US"><a
+
-
  href="http://lifesc.lzu.edu.cn/category-44.html">Home</a></span></b></span><b
+
-
  style='mso-bidi-font-weight:normal'><span style='color:white;
+
-
  mso-themecolor:background1' lang="EN-US" xml:lang="EN-US">
+
-
          <o:p></o:p>
+
-
        </span></b></p></td>
+
-
      <td width="137" style='width:102.5pt;background:#FFC000;padding:.75pt .75pt .75pt .75pt'><p class="MsoNormal" align="center" style='text-align:center'><span
+
-
  class="MsoHyperlink"><b style='mso-bidi-font-weight:normal'><span lang="EN-US" xml:lang="EN-US"><a
+
-
  href="https://2013.igem.org/Team:LZU-China">Team</a></span></b></span><b
+
-
  style='mso-bidi-font-weight:normal'><span style='color:white;
+
-
  mso-themecolor:background1' lang="EN-US" xml:lang="EN-US">
+
-
          <o:p></o:p>
+
-
        </span></b></p></td>
+
-
      <td width="155" style='width:116.55pt;background:#FFC000;padding:.75pt .75pt .75pt .75pt'><p class="MsoNormal" align="center" style='text-align:center'><span
+
-
  class="MsoHyperlink"><b style='mso-bidi-font-weight:normal'><span lang="EN-US" xml:lang="EN-US"><a
+
-
  href="https://igem.org/Team.cgi?id=1523">Official profile</a></span></b></span><b
+
-
  style='mso-bidi-font-weight:normal'><span style='color:white;
+
-
  mso-themecolor:background1' lang="EN-US" xml:lang="EN-US">
+
-
          <o:p></o:p>
+
-
        </span></b></p></td>
+
-
      <td width="118" style='width:88.45pt;background:#FFC000;padding:.75pt .75pt .75pt .75pt'><p class="MsoNormal" align="center" style='text-align:center'><span
+
-
  class="MsoHyperlink"><b style='mso-bidi-font-weight:normal'><span lang="EN-US" xml:lang="EN-US"><a
+
-
  href="http://lifesc.lzu.edu.cn/category-44.html">News &amp; Notes</a></span></b></span><b
+
-
  style='mso-bidi-font-weight:normal'><span style='color:white;
+
-
  mso-themecolor:background1' lang="EN-US" xml:lang="EN-US">
+
-
          <o:p></o:p>
+
-
        </span></b></p></td>
+
-
      <td width="136" style='width:101.8pt;background:#FFC000;padding:.75pt .75pt .75pt .75pt'><p class="MsoNormal" align="center" style='text-align:center'><b
+
-
  style='mso-bidi-font-weight:normal'><span style='color:white;
+
-
  mso-themecolor:background1' lang="EN-US" xml:lang="EN-US"><a href="https://2013.igem.org/Team:LZU-China"><span
+
-
  lang="EN-US" xml:lang="EN-US"><span lang="EN-US" xml:lang="EN-US"><span lang="EN-US" xml:lang="EN-US"><span lang="EN-US" xml:lang="EN-US">        LZU-China 2013</span></span></span></span></a>
+
-
        <o:p></o:p>
+
-
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  color:black;mso-themecolor:text1' lang="EN-US" xml:lang="EN-US">LanZhou</span></b></span><b
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  mso-bidi-font-size:12.0pt;line-height:150%;color:black;mso-themecolor:text1' lang="EN-US" xml:lang="EN-US"> University | 222 <span class="SpellE">TianShui</span> South Road, <span
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          <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" >&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;THE NOTEBOOK OF WET LAB GROUP A</td>
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          <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg"  width="57%"><p>&nbsp;</p>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2014.5.16-5.23<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The pre-experience of the growth of three  strains(<em>Shewanella onedensis,</em> <em>Pseudomonas aeruginosa </em>and LZU-3, which is separated in Li&rsquo;s  lab )in different PNP concentration and  Cr(VI) concentration.</p>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="z.da1.png" width="700" height="173" /></p>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2014.5.24-5.31<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Measurement for three strains&rsquo;(<em>Shewanella onedensis,</em> <em>Pseudomonas aeruginosa </em>and LZU-3, which is separated in Li&rsquo;s  lab ) Cr(VI) reduction activity.</p>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="z.a2.png" width="467" height="605" /></p>
 +
<p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2014.6.1-2014.6.8<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.Another measurement for three strains&rsquo;(as above  said) Cr(VI) reduction activity.</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="z.a3.png" width="569" height="482" /></p>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.Construction of the phylogeny tree containing the LZU-3 </p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="z.4.png" width="654" height="503" /></p>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2014.6.8-2014.7.24<br />
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The PCR cycling for BBa_I723020 and BBa_I723133</p>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1. The primers(synthesized by BGI,shenzhen)</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.1 Promoter of BBa_I723133 <br />
 +
              The forward primer (5&rsquo;  TATACTAGTCTGGGGCGAGAGGCGACGAC 3&rsquo;) contained a SpeI cutsite <br />
 +
              The reverse primer (5&rsquo;  GGGGAATTCTAGAATGTGGGCTGCTTGGTG 3&rsquo;) contained a EcoRI and XbaI cutsite. <br />
 +
              2313bp</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.2 BBa_I723020<br />
 +
              The forward primer  (5&rsquo;GGTGCTGCAGACTAGTATTGAAGGGTCACCACTATTTTTATTTTA 3&rsquo;) containes SpeI and PstI  cutsites <br />
 +
              the reverse primer (5&rsquo;  ATGAATTCTCTAGACCTGCTGGAGGGCGTGAAC 3&rsquo;) containes EcoRI and XbaI cutsites. <br />
 +
              340bp</p>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.The  PCR system</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;0.2&mu;L ExTaq.<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1&mu;L bacterial liquid<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1&mu;L dNTP<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1&mu;L forward primer<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1&mu;L reverse primer<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.5&mu;L 10x Buffer<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;18.5&mu;L ddH2O</p>
 +
<p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2014.7.31-8.1<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Pre-experience for the function of BBa_K381001.<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;BBa_K381001 transformation to E.coli DH5 alpha<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;There are two groups to test the green fluorescence under microscope:<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(These groups' total sizes are all 20&mu;L, had been shaken for 2hours before observation,400x)<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;a.bacterial liquid with 10mM PNP concentration.<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;b.bacterial liquid with 20mM PNP concentration.<br />
 +
            </p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="z.5.png" width="704" height="307" /></p>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2014.8.2-2014.8.6<br />
 +
              Pre-experience for the function of BBa_K381001.<br />
 +
              BBa_K381001 transformation to E.coli DH5 alpha<br />
 +
              There are four groups to test the green fluorescence under microscope:<br />
 +
              (These groups' total sizes are all 20&mu;L, had been shaken for 2hours before observation,1000x)<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;a.bacterial liquid with 10mM PNP concentration.b.<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;b.bacterial liquid with ddH2O<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;c.bacterial liquid with 10mM KNO3<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;d.bacterial liquid with 10mM KCl.<br />
 +
            </p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="z.a6.png" width="455" height="340" /></p>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2014.8.25-9.21<br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.Construction of BBa_K1523101<br />
 +
            </p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="z.a7.png" width="489" height="90" /></p>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The size of the whole part is about 3700bp(without plasmid), we test the assembling result by PCR(sense:5'---TTCCCATCTATAATCCTCCCTGATTCTTCG---3';anti-sense:5'---GAATTCTCTAGATTACAACTGTTGTTCAAGCTGTT---3'). From the gel picture we can see the size is right.</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="z.a8.png" width="643" height="305" /></p>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.Construction of BBa_K1523024(yieF) and BBa_K1523005(chrR)</p>
 +
<p>&nbsp;</p>
 +
<p>&nbsp;</p>
 +
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2014.9.21-10.2<br />
 +
   &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Construction of BBa_K1523021(<em>nahR</em>),  K1523022(<em>nahE</em>) and K1523023(<em>nahG</em>)</p>
 +
<p>
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<p>You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well. </p>
 
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Revision as of 18:32, 16 October 2014

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> LZU-China 2014

 
 
 

 

 

 

 

 

 

 

 

 
 
 

 

 

 

        THE NOTEBOOK OF WET LAB GROUP A
 

 

 

        2014.5.16-5.23
        The pre-experience of the growth of three strains(Shewanella onedensis, Pseudomonas aeruginosa and LZU-3, which is separated in Li’s lab )in different PNP concentration and Cr(VI) concentration.

 

        

 

 

        2014.5.24-5.31
        Measurement for three strains’(Shewanella onedensis, Pseudomonas aeruginosa and LZU-3, which is separated in Li’s lab ) Cr(VI) reduction activity.

 

                                

 

        2014.6.1-2014.6.8
        1.Another measurement for three strains’(as above said) Cr(VI) reduction activity.

                 

 

        2.Construction of the phylogeny tree containing the LZU-3

             

 

 

          2014.6.8-2014.7.24
          The PCR cycling for BBa_I723020 and BBa_I723133

 

       1. The primers(synthesized by BGI,shenzhen)

       1.1 Promoter of BBa_I723133
The forward primer (5’ TATACTAGTCTGGGGCGAGAGGCGACGAC 3’) contained a SpeI cutsite
The reverse primer (5’ GGGGAATTCTAGAATGTGGGCTGCTTGGTG 3’) contained a EcoRI and XbaI cutsite.
2313bp

       1.2 BBa_I723020
The forward primer (5’GGTGCTGCAGACTAGTATTGAAGGGTCACCACTATTTTTATTTTA 3’) containes SpeI and PstI cutsites
the reverse primer (5’ ATGAATTCTCTAGACCTGCTGGAGGGCGTGAAC 3’) containes EcoRI and XbaI cutsites.
340bp

 

      2.The PCR system

            0.2μL ExTaq.
            1μL bacterial liquid
            1μL dNTP
            1μL forward primer
            1μL reverse primer
            2.5μL 10x Buffer
            18.5μL ddH2O

 

          2014.7.31-8.1
      Pre-experience for the function of BBa_K381001.
      BBa_K381001 transformation to E.coli DH5 alpha
      There are two groups to test the green fluorescence under microscope:
      (These groups' total sizes are all 20μL, had been shaken for 2hours before observation,400x)
                  a.bacterial liquid with 10mM PNP concentration.
                  b.bacterial liquid with 20mM PNP concentration.

          

 

          2014.8.2-2014.8.6
Pre-experience for the function of BBa_K381001.
BBa_K381001 transformation to E.coli DH5 alpha
There are four groups to test the green fluorescence under microscope:
(These groups' total sizes are all 20μL, had been shaken for 2hours before observation,1000x)
      a.bacterial liquid with 10mM PNP concentration.b.
      b.bacterial liquid with ddH2O
      c.bacterial liquid with 10mM KNO3
      d.bacterial liquid with 10mM KCl.

                              

 

          2014.8.25-9.21
      1.Construction of BBa_K1523101

                              

 

      The size of the whole part is about 3700bp(without plasmid), we test the assembling result by PCR(sense:5'---TTCCCATCTATAATCCTCCCTGATTCTTCG---3';anti-sense:5'---GAATTCTCTAGATTACAACTGTTGTTCAAGCTGTT---3'). From the gel picture we can see the size is right.

             

 

      2.Construction of BBa_K1523024(yieF) and BBa_K1523005(chrR)

 

 

          2014.9.21-10.2
      Construction of BBa_K1523021(nahR), K1523022(nahE) and K1523023(nahG)