Team:Korea U Seoul/Notebook sub
From 2014.igem.org
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+ | <div class="title"> | ||
+ | <div class="title_"> | ||
+ | 2014 IGEM 6th minutes | ||
+ | </div> | ||
+ | <div class="left_line"></div><div class="right_line"></div> | ||
+ | </div> | ||
+ | <div class="content_"> | ||
+ | <p style="font-weight: bold; font-size:20px;"> | ||
+ | Presentation of ideas | ||
+ | </p><br /> | ||
+ | <ol type="1"/> | ||
+ | <li>JUYOUNG</li> | ||
+ | <p>- Siderophore : absorb iron ion FUR system ) Ferric Uptake Regulator.</p> | ||
+ | <p>- Aflatoxin </p> | ||
+ | <li>MINSEOB</li> | ||
+ | <p>- Shiga toxin @ shiga toxin producing E. coli: cause hemolytic-uremic syndrome (Proprotein convertases :change proprotein to protein )</p> | ||
+ | <p>- Messages in a bubble : outer membrane vesicle (OMV)</p> | ||
+ | <p>- Collaboration : proprotein caspases (one of serine proteases, subtilisin serine proteases) , send subtilisin to STEC (destroyed shiga toxin will be sent to eukaryotic, but it won’t work. )</p> | ||
+ | <p>- Develop experiments of Paris </p> | ||
+ | <li>JIHEE</li> | ||
+ | <p>- pectinase production from E. coli</p> | ||
+ | <p>- Cellulase+pectinase-> more degradation of pulp</p> | ||
+ | <p>- Use residuum of fruits by pectinase </p> | ||
+ | <li>HYEYEONG</li> | ||
+ | <p>- Removing bad smells of food junks </p> | ||
+ | <p>- the main components of bad smells include nitrogen->regulate metabolism </p> | ||
+ | <li>HYUKJIN</li> | ||
+ | <p>- Cellulolytic <i>E. coli</i> : cellulose-> oligo-saccharides-> disaccharide -> glucose(Use ocean microbes which can degrade cellulose to disaccharides, Regulating promoter for find ultimate media.)</p> | ||
+ | <p>- Filamentus bacteria phage </p> | ||
+ | <p>- <i>C. glutamioum</i>: agarose fili designing: new microbes, not <i>E. coli</i> </p> | ||
+ | </ol><br /> | ||
+ | <p style="font-weight: bold; font-size:20px;"> | ||
+ | Next week | ||
+ | </p><br /> | ||
+ | <ul> | ||
+ | <li>Proteins which can attach to filamentus bacteria phage</li> | ||
+ | </ul><br /> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="title"> | ||
+ | <div class="title_"> | ||
+ | 2014 IGEM 7th minutes | ||
+ | </div> | ||
+ | <div class="left_line"></div><div class="right_line"></div> | ||
+ | </div> | ||
+ | <div class="content_"> | ||
+ | <p>Dine together: Le’guette 60500 korean dollars</p> | ||
+ | <p style="font-weight: bold; font-size:20px;"><br /> | ||
+ | discussion | ||
+ | </p><br /> | ||
+ | <ol type="1"/> | ||
+ | <li>OMV</li> | ||
+ | <li>Agarose</li> | ||
+ | <li>Bio informatics</li> | ||
+ | <li>Micro Dynamics</li> | ||
+ | </ol><br /> | ||
+ | </div> | ||
+ | |||
+ | <div class="title"> | ||
+ | <div class="title_"> | ||
+ | 2014 IGEM 8th minutes | ||
+ | </div> | ||
+ | <div class="left_line"></div><div class="right_line"></div> | ||
+ | </div> | ||
+ | <div class="content_"> | ||
+ | <p style="font-weight: bold; font-size:20px;"><br /> | ||
+ | discussion | ||
+ | </p><br /> | ||
+ | <ol type="1"/> | ||
+ | <li>OMV dynamics</li> | ||
+ | <li>Reappearance experiment -OMV </li> | ||
+ | <li>Targeting -OMV </li> | ||
+ | <li>Agarase + other enzyme metabolic pathway </li> | ||
+ | <li>Cellulose degradation –agarose degradation</li> | ||
+ | </ol><br /> | ||
+ | </div> | ||
+ | |||
+ | <div class="title"> | ||
+ | <div class="title_"> | ||
+ | 2014 IGEM 9th minutes | ||
+ | </div> | ||
+ | <div class="left_line"></div><div class="right_line"></div> | ||
+ | </div> | ||
+ | <div class="content_"> | ||
+ | <p style="font-weight: bold; font-size:20px;"><br /> | ||
+ | Ideas | ||
+ | </p><br /> | ||
+ | <ol type="1"/> | ||
+ | <li>OMV reservation</li> | ||
+ | <li>Circular per-mutation protein </li> | ||
+ | <p>- advantage : high effectiveness</p> | ||
+ | <p>- high tolerance enzyme : endothermic </p> | ||
+ | <p>- new substrate-specificity expression </p> | ||
+ | </ol><br /> | ||
+ | <p style="font-weight: bold; font-size:20px;"><br /> | ||
+ | Human practice | ||
+ | </p><br /> | ||
+ | <p>Apply Univ-expo</p><br /> | ||
+ | <p style="font-weight: bold; font-size:20px;"><br /> | ||
+ | Next week | ||
+ | </p><br /> | ||
+ | <ol type="1"/> | ||
+ | <li>minseob –study the structure, what kind of protein we will attach</li> | ||
+ | </ol><br /> | ||
+ | </div> | ||
+ | |||
+ | <div class="title"> | ||
+ | <div class="title_"> | ||
+ | 2014 IGEM 10th minutes | ||
+ | </div> | ||
+ | <div class="left_line"></div><div class="right_line"></div> | ||
+ | </div> | ||
+ | <div class="content_"> | ||
+ | <p style="font-weight: bold; font-size:20px;"><br /> | ||
+ | NGS | ||
+ | </p><br /> | ||
+ | <p>Hyukjjin’s presentation</p> | ||
+ | <p style="font-weight: bold; font-size:20px;"><br /> | ||
+ | Enzyme fuel cell | ||
+ | </p><p>reference</p><br /> | ||
+ | <ol type="1"/> | ||
+ | <li>http://www.sony.net/SonyInfo/csr/SonyEnvironment/technology/bio_battery2.html</li> | ||
+ | <li>http://www.nature.com/ncomms/2014/140121/ncomms4026/full/ncomms4026.html</li> | ||
+ | <li>https://2013.igem.org/Team:Bielefeld-Germany</li> | ||
+ | </ol><br /> | ||
+ | <p>Apply Univ-expo</p><br /> | ||
+ | <p style="font-weight: bold; font-size:20px;"><br /> | ||
+ | Human practice | ||
+ | </p><br /> | ||
+ | <ol type="1"/> | ||
+ | <li>Waiting contact from Univ EXPO. </li> | ||
+ | </ol><br /> | ||
+ | </div> | ||
+ | |||
+ | <div class="title"> | ||
+ | <div class="title_"> | ||
+ | 2014 IGEM 12th minutes | ||
+ | </div> | ||
+ | <div class="left_line"></div><div class="right_line"></div> | ||
+ | </div> | ||
+ | <div class="content_"> | ||
+ | <p style="font-weight: bold; font-size:20px;"><br /> | ||
+ | Ideas | ||
+ | </p><br /> | ||
+ | <ol type="1"/> | ||
+ | <li>Kyungwoo - Microbial behavior control</li> | ||
+ | </ol><br /> | ||
+ | <p style="font-weight: bold; font-size:20px;"><br /> | ||
+ | Next week | ||
+ | </p><br /> | ||
+ | <ol type="1"/> | ||
+ | <li>Evolutionary dynamics study </li> | ||
+ | </ol><br /> | ||
+ | </div> | ||
</div> | </div> | ||
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Revision as of 01:48, 18 October 2014
Meeting
- First meeting with 2014 kuas team members. Discussed about what is kuas, synthetic biology and igem.
Information
- Synthetic biology homepage : http://syntheticbiology.org
- Part legistry page : http://part.igem.org
Homework
- investigate about subject of2008, 2009 igem teams investigate about subject of 2008, 2009 igem teams
Study of igem 2008~2009 colleges
- Outer membrane vesicles (OMV) : gram negative bacteria like E. coli have Outer membrane vesicles (OMV), which can transfer toxin to other bacteria or host strains. Omv is composed of bi-lipid layer with water soluble core, so it can transfer both water soluble materials and lipid soluble materials. Omv is a strong candidate in long-distance messaging.
- Different color expression by density
- Check the expression by density divided strong, wild, weak Lock & key, Jammer
- Formation of hybrid-promoter, light-sensing, biomaterial production
- RNA double helix come untied when increase the heat.
- using NhaA, Urea sensor
Tips of instrutor
Discussion of direction
Study presentation
- Siderosphore : Fe3+ absorb, high reducing agent. =>use ocean microbes to greenhouse effect, pathogen control, biofuel
- Degradation Aplatoxin
- GABA : neuro-material.
- T3SS(Type 3 secretion system) : related to plant immunity. Mechanism has been found.
- Pathogen microorganism sensor : use metabolic product as marker like enterotoxin or virulence factor
- PCN(antifungal product)
- Shiga toxin producing E. coli : can develop to cell to cell communication. Herein.
- checker of cosmetics: ex) PH sensor
- dye : E. coli producing indigo
- Pectinase+ beta-chrotin, E. coli producing 3-4 nutrients-> making juice
- bio-filter
- removal odors of food junks : ex)remove pathogenity.
- Soil sensor
Next week
- minseob- GABA, Shiga toxin, components of cosmetics
- jihee- effectiveness of making juice
- hyeyeong- effectiveness of removing bad smells and probability of removing pathogenity
- juyeong- about iron ion, removing ground torpedo microbes
About ideas
- extension of siderosphore idea
- papers of Sigatoxin
- papers about pection production
- papers of streptomycetes, actinomycetes
Next week
- kyungwoo- feedback to papers
- others- get feedbacks and more study about each idea.
Schedule
Fix idea until middle of January
Accounts
In |
Korean dollar |
Out |
Korean dollar |
2013 |
|
Team registration fee |
300 |
left |
100 |
Participation fee |
250 |
KOFST |
900 |
plane |
1020 |
CCP |
250 |
abode |
360 |
2014 |
|
Team tea-shirts |
30 |
CCP |
250 |
|
|
KOFST |
150 |
|
|
Self-administration of KU |
60 |
|
|
Mycroryza |
10 |
|
|
festival |
30 |
|
|
fee |
20 |
|
|
sum |
1860 |
sum |
1960 |
Presentation of ideas
- Siderophore : absorb iron ion FUR system ) Ferric Uptake Regulator.
- Aflatoxin
- Shiga toxin @ shiga toxin producing E. coli: cause hemolytic-uremic syndrome (Proprotein convertases :change proprotein to protein )
- Messages in a bubble : outer membrane vesicle (OMV)
- Collaboration : proprotein caspases (one of serine proteases, subtilisin serine proteases) , send subtilisin to STEC (destroyed shiga toxin will be sent to eukaryotic, but it won’t work. )
- Develop experiments of Paris
- pectinase production from E. coli
- Cellulase+pectinase-> more degradation of pulp
- Use residuum of fruits by pectinase
- Removing bad smells of food junks
- the main components of bad smells include nitrogen->regulate metabolism
- Cellulolytic E. coli : cellulose-> oligo-saccharides-> disaccharide -> glucose(Use ocean microbes which can degrade cellulose to disaccharides, Regulating promoter for find ultimate media.)
- Filamentus bacteria phage
- C. glutamioum: agarose fili designing: new microbes, not E. coli
Next week
- Proteins which can attach to filamentus bacteria phage
Dine together: Le’guette 60500 korean dollars
discussion
discussion
Ideas
- advantage : high effectiveness
- high tolerance enzyme : endothermic
- new substrate-specificity expression
Human practice
Apply Univ-expo
Next week
NGS
Hyukjjin’s presentation
Enzyme fuel cell
reference
Apply Univ-expo
Human practice
Ideas
Next week