Team:Kent/background
From 2014.igem.org
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- | <td><div align="justify">Our project is specifically aimed at terpenoid production in <i>E.coli</i>. These compounds particularly in plants give off scents when produced and we hope to improve on the yield that is obtained during current chemical production techniques. <br> | + | <td><div align="justify">Our project is specifically aimed at terpenoid production in <i>E.coli</i>. These compounds particularly in plants give off scents when produced and we hope to improve on the yield that is obtained during current chemical production techniques. <br> |
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Terpenoids are characteristic molecules that derive from the condensation of 5-carbon isoprene units. They play major roles in plants such as growth regulation, signalling, and defense mechanisms. Additionally terpenoids have a characteristic aroma. <br> | Terpenoids are characteristic molecules that derive from the condensation of 5-carbon isoprene units. They play major roles in plants such as growth regulation, signalling, and defense mechanisms. Additionally terpenoids have a characteristic aroma. <br> | ||
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- | There are two metabolic pathways used to synthesize terpenoids: the mevalonate pathway in plants and the non mevalonate pathway in | + | There are two metabolic pathways used to synthesize terpenoids: the mevalonate pathway in plants and the non mevalonate pathway in <i>E.coli</i>l (fig1). Both pathways ultimately lead to compounds: Isopentanyl pyrophosphate and dimethyl Isoprenyl pyrophosphate which are further converted into geranyl pyrophosphate farnesyl pyrophosphate to synthesize terpenes.</div></td> |
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Due to these molecules possessing fragrance qualities, they are used in industry for the production of perfumes, cosmetics, household products, etc. <br> | Due to these molecules possessing fragrance qualities, they are used in industry for the production of perfumes, cosmetics, household products, etc. <br> | ||
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- | Our project focuses on the synthesis of selected terpene fragrant compounds: zingiberene, R-linalool and limonene, by expressing each enzyme in | + | Our project focuses on the synthesis of selected terpene fragrant compounds: zingiberene, R-linalool and limonene, by expressing each enzyme in <i>E.coli.</i></p> |
<p align="justify"><strong>Limonene synthase:<br> | <p align="justify"><strong>Limonene synthase:<br> | ||
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Latest revision as of 01:36, 18 October 2014
Background |
Our project is specifically aimed at terpenoid production in E.coli. These compounds particularly in plants give off scents when produced and we hope to improve on the yield that is obtained during current chemical production techniques. Terpenoids are characteristic molecules that derive from the condensation of 5-carbon isoprene units. They play major roles in plants such as growth regulation, signalling, and defense mechanisms. Additionally terpenoids have a characteristic aroma. There are two metabolic pathways used to synthesize terpenoids: the mevalonate pathway in plants and the non mevalonate pathway in E.colil (fig1). Both pathways ultimately lead to compounds: Isopentanyl pyrophosphate and dimethyl Isoprenyl pyrophosphate which are further converted into geranyl pyrophosphate farnesyl pyrophosphate to synthesize terpenes. |
Fig1. Mevalonate and Non-Mevalonate pathway. two pathways from different organisms Limonene synthase: |
Use of zingiberene: R-linalool Synthase: |
Production:
References: |