Team:KAIT Japan/Results

From 2014.igem.org

(Difference between revisions)
(GFP+HlyA and HlyA+GFP)
(GFP+HlyA and HlyA+GFP)
Line 101: Line 101:
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==
=='''<font size="5">GFP+HlyA and HlyA+GFP</font>'''==
<font size=4>
<font size=4>
-
GFP ligated with HlyA.Length of GFP+HlyA is 920bp
+
GFP ligated with HlyA.Length of GFP+HlyA is 920bp.
Following figure 3 is GFP+HlyA.
Following figure 3 is GFP+HlyA.
-
Following figure 4 is HlyA+GFP
+
Following figure 4 is HlyA+GFP.
We submitted these parts.
We submitted these parts.
But the function of these parts,we were not able to confirm it.
But the function of these parts,we were not able to confirm it.

Revision as of 08:42, 17 October 2014

ロゴ2.png

KAIT Japan2013 Kanako.png

KAIT Japan 2014 iGEMRogo.png
New home t.jpg

Home

New Team1.jpg

Team

New 考える人.jpg

Project

New 歯車.jpg

Parts

New 111111111111111.jpg

Protocol

Ff.jpg

Notebook

New 結果.jpg

Results

New new 危険.jpg

Safety

New new ひと.jpg

Human Practice

Contents

Results


GFP+HlyA(BBa_K1414000) and HlyA+GFP(BBA_K1414001)

GFP.jpg

[Figure 1]

GFP

We used "BBa_E0040". Length of "BBa_E0040"is 920bp. Following figure 1 is result of the GFP PCR. As a result,We were able to confirm a band about 920bp by GFP PCR.



HlyA.jpg

[Figure 2]

HlyA

We used "BBa_K554002". Length of "BBa_K554002" is 186bp. Following figure 2 is result of the HlyA PCR. As a result,We were able to confirm a band about 186bp by HlyA PCR.



HlyA.jpg

[Figure 3]

GFP+HlyA.jpg

[Figure 4]

GFP+HlyA and HlyA+GFP

GFP ligated with HlyA.Length of GFP+HlyA is 920bp. Following figure 3 is GFP+HlyA. Following figure 4 is HlyA+GFP. We submitted these parts. But the function of these parts,we were not able to confirm it. Because we did not have time to experiment.

Creating parts of STAT3



Creating parts of IL-10α,IL-10β



Creating parts of HRV,IL-5Ra,IL-5Rb,AraC,Arac Promoter

GFP.jpg

[Figure 1]

Example.jpg

[Figure 2]

GFP