Team:KAIT Japan/Notebook

From 2014.igem.org

(Difference between revisions)
Line 63: Line 63:
:<big>'''Date:8/25'''</big> PCR and Electrophoresis and Restriction
:<big>'''Date:8/25'''</big> PCR and Electrophoresis and Restriction
:<big>'''Date:8/26'''</big> Insert gene into TAvectar and Transformation
:<big>'''Date:8/26'''</big> Insert gene into TAvectar and Transformation
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:<big>'''Date:8/26'''</big> Blue white Selection and Electrophoresis and DNA Extraction
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:<big>'''Date:8/26'''</big> Blue white Selection and Electrophoresis(8/25 Restriction) and DNA Extraction
-
 
+
=='''Creating parts of IL-10α,IL-10β,STAT3'''==
 +
:<big>'''Date:8/19'''</big> PCR(8/18 Miniprep) and Electrophoresis
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:<big>'''Date:8/20'''</big> PCR(Lowered 2℃ from Tm value.) and Electrophoresis
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:<big>'''Date:8/21'''</big> PCR and Electrophoresis
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:<big>'''Date:8/22'''</big> PCR and Electrophoresis
 +
:<big>'''Date:8/25'''</big> PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)
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:<big>'''Date:8/26'''</big> PCR and Electrophoresis(Using a mutation primer.)
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Revision as of 04:31, 29 August 2014

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KAIT Japan2013 Kanako.png

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Home

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Team

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Project

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Parts

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Protocol

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Notebook

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Results

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Safety

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Human Practice


Notebook

We made an experiment every day. It was process at trial and error.

Basic task

Date:8/18 Miniprep

Creating parts of HlyA and GFP

Date:8/22 The refinement of DNA and PCR and Electrophoresis
Date:8/25 PCR and Electrophoresis and Restriction
Date:8/26 Insert gene into TAvectar and Transformation
Date:8/26 Blue white Selection and Electrophoresis(8/25 Restriction) and DNA Extraction

Creating parts of IL-10α,IL-10β,STAT3

Date:8/19 PCR(8/18 Miniprep) and Electrophoresis
Date:8/20 PCR(Lowered 2℃ from Tm value.) and Electrophoresis
Date:8/21 PCR and Electrophoresis
Date:8/22 PCR and Electrophoresis
Date:8/25 PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)
Date:8/26 PCR and Electrophoresis(Using a mutation primer.)