Team:KAIT Japan/Notebook

From 2014.igem.org

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(→Creating parts of IL-10AR,IL-10BR and STAT3)
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:<big>'''Date:8/18'''</big> Miniprep
:<big>'''Date:8/18'''</big> Miniprep
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=='''Creating parts of HlyA and pGLO'''==
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=='''Creating parts of HlyA and GFP'''==
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:<big>'''Date:8/8'''</big> Design of the primer
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:<big>'''Date:8/22'''</big> The refinement of DNA and PCR and Electrophoresis
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:<big>'''Date:8/9'''</big> Transformation
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:<big>'''Date:8/25'''</big> PCR and Electrophoresis and Restriction
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:<big>'''Date:8/12'''</big> Subcluture
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:<big>'''Date:8/26'''</big> Insert gene into TAvectar and Transformation
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:<big>'''Date:8/13'''</big> Colony PCR of HlyA and Electrophoresis
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:<big>'''Date:8/26'''</big> Blue white Selection and Electrophoresis and DNA Extraction
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:<big>'''Date:8/16'''</big> Transformation,Subcluture and Electrophoresis
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:<big>'''Date:8/22'''</big> PCR of HlyA
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:<big>'''Date:8/27'''</big> PCR and Electrophoresis
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:<big>'''Date:9/11'''</big> The refinement of DNA
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:<big>'''Date:9/11'''</big> Infusion
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=='''Creating parts of GFP'''==
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:<big>'''Date:8/20'''</big> Colony PCR and Electrophoresis
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:<big>'''Date:8/21'''</big> Colony PCR and Electrophoresis
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:<big>'''Date:8/23'''</big> PCR and Electrophoresis
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:<big>'''Date:8/24'''</big> PCR and Electrophoresis
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:<big>'''Date:8/28'''</big> PCR
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:<big>'''Date:8/29'''</big> PCR and Electrophoresis
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:<big>'''Date:8/30'''</big> Subcluture and PCR
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:<big>'''Date:9/5'''</big> Subcluture
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:<big>'''Date:9/6'''</big> Colony PCR and Electrophoresis
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:<big>'''Date:9/9'''</big> PCR
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:<big>'''Date:9/10'''</big> PCR
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:<big>'''Date:9/18'''</big> PCR and Transformation
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:<big>'''Date:9/19'''</big> Colony PCR
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=='''Creating parts of IL-10AR,IL-10BR and STAT3'''==
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:<big>'''Date:8/19'''</big> PCR(mini prep) and Electrophoresis
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:<big>'''Date:8/20'''</big> PCR and Electrophoresis
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:<big>'''Date:8/21'''</big>  PCR and Electrophoresis
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:<big>'''Date:8/22'''</big>  PCR and Electrophoresis
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:<big>'''Date:8/25'''</big> PCR and Electrophoresis
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:<big>'''Date:8/26'''</big> PCR and Electrophoresis
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|}
|}

Revision as of 04:11, 29 August 2014

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Home

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Team

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Project

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Parts

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Protocol

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Notebook

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Results

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Safety

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Human Practice


Notebook

We made an experiment every day. It was process at trial and error.

Basic task

Date:8/18 Miniprep

Creating parts of HlyA and GFP

Date:8/22 The refinement of DNA and PCR and Electrophoresis
Date:8/25 PCR and Electrophoresis and Restriction
Date:8/26 Insert gene into TAvectar and Transformation
Date:8/26 Blue white Selection and Electrophoresis and DNA Extraction
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