Team:KAIT Japan/Notebook

From 2014.igem.org

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(→Creating parts of IL-10AR,IL-10BR and STAT3)
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:<big>'''Date:9/19'''</big> Colony PCR  
:<big>'''Date:9/19'''</big> Colony PCR  
=='''Creating parts of IL-10AR,IL-10BR and STAT3'''==
=='''Creating parts of IL-10AR,IL-10BR and STAT3'''==
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:<big>'''Date:8/30'''</big> Colony PCR and Electrophoresis
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:<big>'''Date:8/19'''</big> PCR(mini prep) and Electrophoresis
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:<big>'''Date:8/31'''</big> Colony PCR and Electrophoresis
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:<big>'''Date:8/20'''</big> PCR and Electrophoresis
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:<big>'''Date:9/5'''</big> Colony PCR and Electrophoresis of IL-10AR and IL-10BR
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:<big>'''Date:8/21'''</big> PCR and Electrophoresis  
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:<big>'''Date:9/6'''</big> Colony PCR and Electrophoresis of STAT3
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:<big>'''Date:8/22'''</big> PCR and Electrophoresis  
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:<big>'''Date:9/6'''</big> PCR and infusion of IL-10AR and IL-10BR
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:<big>'''Date:8/25'''</big> PCR and Electrophoresis
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:<big>'''Date:9/9'''</big> Infusion of IL-10R
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:<big>'''Date:8/26'''</big> PCR and Electrophoresis
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:<big>'''Date:9/9'''</big> PCR of STAT3
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:<big>'''Date:9/11'''</big> Infusion of IL-10R and STAT3
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Revision as of 03:42, 29 August 2014

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Home

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Team

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Project

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Parts

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Protocol

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Notebook

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Results

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Safety

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Human Practice


Notebook

We made an experiment every day. It was process at trial and error.

Basic task

Date:8/18 Miniprep

Creating parts of HlyA and pGLO

Date:8/8 Design of the primer
Date:8/9 Transformation
Date:8/12 Subcluture
Date:8/13 Colony PCR of HlyA and Electrophoresis
Date:8/16 Transformation,Subcluture and Electrophoresis
Date:8/22 PCR of HlyA
Date:8/27 PCR and Electrophoresis
Date:9/11 The refinement of DNA
Date:9/11 Infusion

Creating parts of GFP

Date:8/20 Colony PCR and Electrophoresis
Date:8/21 Colony PCR and Electrophoresis
Date:8/23 PCR and Electrophoresis
Date:8/24 PCR and Electrophoresis
Date:8/28 PCR
Date:8/29 PCR and Electrophoresis
Date:8/30 Subcluture and PCR
Date:9/5 Subcluture
Date:9/6 Colony PCR and Electrophoresis
Date:9/9 PCR
Date:9/10 PCR
Date:9/18 PCR and Transformation
Date:9/19 Colony PCR

Creating parts of IL-10AR,IL-10BR and STAT3

Date:8/19 PCR(mini prep) and Electrophoresis
Date:8/20 PCR and Electrophoresis
Date:8/21 PCR and Electrophoresis
Date:8/22 PCR and Electrophoresis
Date:8/25 PCR and Electrophoresis
Date:8/26 PCR and Electrophoresis
Retrieved from "http://2014.igem.org/Team:KAIT_Japan/Notebook"