Team:KAIT Japan/Notebook

From 2014.igem.org

(Difference between revisions)
Line 52: Line 52:
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ACFA58; text-align:left"
{| style="width:98.5%; margin-left:0.2em; border-color:#00BFFF; border-style:solid; border-collapse:collapse; background-color:#ACFA58; text-align:left"
| style="width:30% style="vertical-align:top" |
| style="width:30% style="vertical-align:top" |
-
 
<br>
<br>
-
 
-
| style="border-left:3px solid #f30" valign="top" |
 
__NOTOC__
__NOTOC__
='''Notebook'''=
='''Notebook'''=

Revision as of 01:50, 29 August 2014

ロゴ2.png

KAIT Japan2013 Kanako.png

KAIT Japan 2014 iGEMRogo.png
Kaitjapan.home2.png

Home

Kaitjapan team2.png

Team

Kaitjapan project2.png

Project

Kaitjapan parts2.png

Parts

Kaitjapan protocol2.png

Protocol

Kaitjapan notebook2.png

Notebook

Kaitjapan results2.png

Results

Kaitjapan safety2.png

Safety

Kaitjapan human practice2.png

Human Practice


Notebook

We made an experiment every day. It was process at trial and error.

Basic task

Date:8/18 Miniprep

Creating parts of HlyA and pGLO

Date:8/8 Design of the primer
Date:8/9 Transformation
Date:8/12 Subcluture
Date:8/13 Colony PCR of HlyA and Electrophoresis
Date:8/16 Transformation,Subcluture and Electrophoresis
Date:8/22 PCR of HlyA
Date:8/27 PCR and Electrophoresis
Date:9/11 The refinement of DNA
Date:9/11 Infusion

Creating parts of GFP

Date:8/20 Colony PCR and Electrophoresis
Date:8/21 Colony PCR and Electrophoresis
Date:8/23 PCR and Electrophoresis
Date:8/24 PCR and Electrophoresis
Date:8/28 PCR
Date:8/29 PCR and Electrophoresis
Date:8/30 Subcluture and PCR
Date:9/5 Subcluture
Date:9/6 Colony PCR and Electrophoresis
Date:9/9 PCR
Date:9/10 PCR
Date:9/18 PCR and Transformation
Date:9/19 Colony PCR

Creating parts of IL-10AR,IL-10BR and STAT3

Date:8/30 Colony PCR and Electrophoresis
Date:8/31 Colony PCR and Electrophoresis
Date:9/5 Colony PCR and Electrophoresis of IL-10AR and IL-10BR
Date:9/6 Colony PCR and Electrophoresis of STAT3
Date:9/6 PCR and infusion of IL-10AR and IL-10BR
Date:9/9 Infusion of IL-10R
Date:9/9 PCR of STAT3
Date:9/11 Infusion of IL-10R and STAT3