Team:KAIT Japan/Notebook

From 2014.igem.org

(Difference between revisions)
(Creating parts of IL-10α,IL-10β)
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=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==
=='''<font size="6">Creating parts of IL-10α,IL-10β</font>'''==
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:<big>'''Date:8/19'''</big> PCR(8/18 Miniprep) and Electrophoresis
+
19'''</big> PCR(8/18 Miniprep) and Electrophoresis
-
:<big>'''Date:8/20~8/22'''</big> PCR(Lowered 2℃ from Tm value.) and Electrophoresis
+
:<big>'''Date:8/20~8/22:<big>'''Date:8/'''</big> PCR(Lowered 2℃ from Tm value.) and Electrophoresis
:<big>'''Date:8/25'''</big> PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)
:<big>'''Date:8/25'''</big> PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)
:<big>'''Date:8/26'''</big> PCR and Electrophoresis(Using a mutation primer.)
:<big>'''Date:8/26'''</big> PCR and Electrophoresis(Using a mutation primer.)
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:<big>'''Date:9/2'''</big>  
:<big>'''Date:9/2'''</big>  
:<big>'''Date:9/8'''</big>  
:<big>'''Date:9/8'''</big>  
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:<big>'''Date:9/9'''</big> Sequence(to To confirm whether variation happened in DNA of IL-10α,IL-10β)
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:<big>'''Date:9/9'''</big> Sequence(to To confirm whether variation happened in DNA of IL-10α,IL-10β):<big>'''Date:8/20~8/22:<big>'''Date:8/'''</big> PCR(Lowered 2℃ from Tm value.) and Electrophoresis
:<big>'''Date:9/10~9/12'''</big>  
:<big>'''Date:9/10~9/12'''</big>  
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Revision as of 08:03, 10 October 2014

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Home

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Team

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Project

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Parts

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Protocol

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Notebook

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Results

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Safety

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Human Practice


Notebook

We made an experiment every day. It was process at trial and error.

Basic task

Date:8/18 Miniprep



Creating parts of HlyA and GFP

Date:8/22 The refinement of DNA and PCR and Electrophoresis
Date:8/25 PCR and Electrophoresis and Restriction
Date:8/26 Insert gene into TAvectar and Transformation
Date:8/26 Blue white Selection and Electrophoresis(8/25 Restriction) and DNA Extraction
Date:8/27 Electrophoresed the DNA that We did refinement on August 26 to confirmed it
Date:





Creating parts of STAT3

Date:8/19 PCR(8/18 Miniprep) and Electrophoresis
Date:8/20~8/22 PCR(Lowered 2℃ from Tm value.) and Electrophoresis
Date:8/25 PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)
Date:8/26 PCR and Electrophoresis(Using a mutation primer.)
Date:8/27 First Variation introduction (with Prime STAR Max)
Date:9/1
Date:9/2
Date:9/8 PCR and Electrophoresis
Date:9/9 Sequence(to To confirm whether variation happened in DNA of STAT3 )
Date:9/10~9/12 PCR and Electrophoresis



Creating parts of IL-10α,IL-10β

19 PCR(8/18 Miniprep) and Electrophoresis

Date:8/20~8/22:Date:8/ PCR(Lowered 2℃ from Tm value.) and Electrophoresis
Date:8/25 PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)
Date:8/26 PCR and Electrophoresis(Using a mutation primer.)
Date:8/27 First Variation introduction (with Prime STAR Max)
Date:9/1
Date:9/2
Date:9/8
Date:9/9 Sequence(to To confirm whether variation happened in DNA of IL-10α,IL-10β):Date:8/20~8/22:Date:8/ PCR(Lowered 2℃ from Tm value.) and Electrophoresis
Date:9/10~9/12