Team:KAIT Japan/Notebook

From 2014.igem.org

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:<big>'''Date:8/26'''</big> Insert gene into TAvectar and Transformation
:<big>'''Date:8/26'''</big> Insert gene into TAvectar and Transformation
:<big>'''Date:8/26'''</big> Blue white Selection and Electrophoresis(8/25 Restriction) and DNA Extraction
:<big>'''Date:8/26'''</big> Blue white Selection and Electrophoresis(8/25 Restriction) and DNA Extraction
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:<big>'''Date:8/27'''</big> Electrophoresed the DNA that We did refinement on August 26 to confirmed it
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:<big>'''Date:
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Revision as of 04:57, 19 September 2014

ロゴ2.png

KAIT Japan2013 Kanako.png

KAIT Japan 2014 iGEMRogo.png
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Home

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Team

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Project

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Parts

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Protocol

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Notebook

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Results

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Safety

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Human Practice


Notebook

We made an experiment every day. It was process at trial and error.

Basic task

Date:8/18 Miniprep



Creating parts of HlyA and GFP

Date:8/22 The refinement of DNA and PCR and Electrophoresis
Date:8/25 PCR and Electrophoresis and Restriction
Date:8/26 Insert gene into TAvectar and Transformation
Date:8/26 Blue white Selection and Electrophoresis(8/25 Restriction) and DNA Extraction
Date:8/27 Electrophoresed the DNA that We did refinement on August 26 to confirmed it
Date:





Creating parts of STAT3

Date:8/19 PCR(8/18 Miniprep) and Electrophoresis
Date:8/20~8/22 PCR(Lowered 2℃ from Tm value.) and Electrophoresis
Date:8/25 PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)
Date:8/26 PCR and Electrophoresis(Using a mutation primer.)
Date:8/27 First Variation introduction (with Prime STAR Max)
Date:9/1
Date:9/2
Date:9/8 PCR and Electrophoresis
Date:9/9 Sequence(to To confirm whether variation happened in DNA of STAT3 )
Date:9/10~9/12 PCR and Electrophoresis



Creating parts of IL-10α,IL-10β

Date:8/19 PCR(8/18 Miniprep) and Electrophoresis
Date:8/20~8/22 PCR(Lowered 2℃ from Tm value.) and Electrophoresis
Date:8/25 PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)
Date:8/26 PCR and Electrophoresis(Using a mutation primer.)
Date:8/27 First Variation introduction (with Prime STAR Max)
Date:9/1
Date:9/2
Date:9/8
Date:9/9 Sequence(to To confirm whether variation happened in DNA of IL-10α,IL-10β)
Date:9/10~9/12