Team:Imperial/Lab-book

From 2014.igem.org

(Difference between revisions)
Line 52: Line 52:
<div class="pure-u-1-3">
<div class="pure-u-1-3">
                     <div class="content content-single">
                     <div class="content content-single">
-
                         <h2>Lab-book 1</h2>
+
                         <h2>Interlab study cloning</h2>
-
                         <p>LINKS</p>
+
                         <p>Precise cloning reaction set-ups are detailed <a href="http://2014.igem.org/wiki/images/a/af/Interlab-study-cloning-lab-work-record.xls">here</a></p>
                          
                          
                     </div>
                     </div>

Revision as of 00:03, 18 October 2014

Imperial iGEM 2014

Lab-books

Introduction

We kept a record of our lab work across a variety of online documents as well as paper based lab-books. Access our online working documents on this page.

General Mixed LabWork

This document contains experimental details for some of the following parts of the project

  • Bulk cellulose growth and processing techniques
  • Coculture experiments
  • G. xylinus isolation and culturing
  • Initial cloning work for Acs operon
  • Preliminary work for isolating curli genes

Primer Inventory

Primers for sequencing, mutagenesis, Gibson Assembly, overlap extension PCR... here is our inventory

Functionalisation cloning

This lab-book is a break-away from the mixed lab-book and contains details of cloning the constructs used in the functionalisation part of our project.

This spreadsheet also details specific digestions and ligation set-ups for these constructs.

Interlab study cloning

Precise cloning reaction set-ups are detailed here

Lab-book 1

LINKS