Team:Imperial/Lab-book

From 2014.igem.org

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                         <h2>Functionalisation cloning</h2>
                         <h2>Functionalisation cloning</h2>
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                         <p><a href="http://2014.igem.org/wiki/images/a/af/Interlab-study-cloning-lab-work-record.xls">This lab-book </a> is a break-away from the mixed lab-book and contains details of cloning the constructs used in the <a href="http://2014.igem.org/Team:Imperial/Functionalisation">functionalisation</a> part of our project</p>
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                         <p><a href="http://2014.igem.org/wiki/images/9/97/Functionalisation_Labwork_record_GC-CM_collated.pdf">This lab-book </a> is a break-away from the mixed lab-book and contains details of cloning the constructs used in the <a href="http://2014.igem.org/Team:Imperial/Functionalisation">functionalisation</a> part of our project</p>  
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<p> <a href="http://2014.igem.org/wiki/images/c/ce/PCR-Digestion-Phos-Ligation-Test_Functionalisation-mainly.xls
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">This spreadsheet</a> also details specific digestions and ligation set-ups for these constructs.
                          
                          
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Revision as of 00:01, 18 October 2014

Imperial iGEM 2014

Lab-books

Introduction

We kept a record of our lab work across a variety of online documents as well as paper based lab-books. Access our online working documents on this page.

General Mixed LabWork

This document contains experimental details for some of the following parts of the project

  • Bulk cellulose growth and processing techniques
  • Coculture experiments
  • G. xylinus isolation and culturing
  • Initial cloning work for Acs operon
  • Preliminary work for isolating curli genes

Primer Inventory

Primers for sequencing, mutagenesis, Gibson Assembly, overlap extension PCR... here is our inventory

Functionalisation cloning

This lab-book is a break-away from the mixed lab-book and contains details of cloning the constructs used in the functionalisation part of our project.

This spreadsheet also details specific digestions and ligation set-ups for these constructs.

Lab-book 1

LINKS

Lab-book 1

LINKS