Team:Imperial

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                        <h1>Imperial College</h1>
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                         <h2>Customisable Ultrafiltration Membranes from Bacterial Cellulose</h2>
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                         <h2>London iGEM</h2>
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                         <h2>The Project</h2>
                         <h2>The Project</h2>
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                            <p>Cellulose is the most abundant organic polymer found in nature. Plants, bacteria and even some animals use its structural and chemical properties for applications such as support, adhesion and flotation. Due to its ubiquity we find cellulose in our everyday lives; from being the main constituent of cotton to uses in medicine and scientific research</p>
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                            <p>Bacterial cellulose (BC) is a natural biomaterial that is of interest in many fields due to its high purity compared with plant-derived cellulose. We are optimising BC biosynthesis in <em>Gluconacetobacter xylinus</em>, transferring the system to <em>E. coli</em>, and functionalising the material using proteins in order to expand its properties. </p> <p>BC has seen use in clothing and health foods, but we develop its application to the global issue of water purification. Rising demand for limited freshwater supplies will lead to more than half of the global population suffering severe water stress by 2030. Improved filtration techniques would help relieve this problem.</p><p> The porosity of BC and our synthetic attachment of contaminant-specific binding and catabolic proteins make for a flexible, modular water filter. Our manufactured biomaterial would augment water recycling and reclamation on local and industrial scales, helping to alleviate water stress.</p>
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                            <div class="more-box "><a href="https://2014.igem.org/Team:Imperial/Project">read more...</a>
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                            <p>Much of the cellulose we use is derived from plants. This is produced as a mixture with other compounds (e.g. lignin and hemicellulose) and so requires an energy intensive purification step to extract the pure cellulose required for many applications. Bacteria offer an alternative means of production that produces a cellulose that is purer and requires less processing.</p>
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                            <p>We want to optimise the production of bacterial cellulose (BC) to increase its viability as a material for a range of potential applications. To do this we aim to engineer Gluconacetobacter xylinum (previously Acetobacter xylinum), a highly-studied native producer of BC. We also hope to transfer our system into E. coli as well as functionalise our biomaterial to give it additional properties.</p>
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                                         <h2>The Team</h2>
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                                         <h2>Implementation</h2>
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                                         <h3>Meet Our Members</h3>
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                                         <h3>Our final idea</h3>
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                                         <div class="more-box"><a href="https://2014.igem.org/Team:Imperial/Students">read more</a>
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                                         <div class="more-box"><a href="https://2014.igem.org/Team:Imperial/Implementation">read more</a>
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                                         <h2>Lorem Ipsum</h2>
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                                         <a href="https://2014.igem.org/Team:Imperial/Results"><h2>Main Achievements</h2></a>
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                                            <li><a href="https://2014.igem.org/Team:Imperial/Functionalisation">Functionalised BC using CBD fusions</a></li>
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                                            <li><a href="https://2014.igem.org/Team:Imperial/Gluconacetobacter">Created a library of <em>G. xylinus</em> parts</a></li>
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                                            <li><a href="https://2014.igem.org/Team:Imperial/Parts">Created over 100 constructs</a></li>
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                                            <li><a href="https://2014.igem.org/Team:Imperial/Gluconacetobacter#genome_sequencing">Sequenced two genomes</a></li>
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                                            <li><a href="https://2014.igem.org/Team:Imperial/EColi">Achieved cellulose production in <em>E.coli</em></a></li>
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                                        <h2>Water Report</h2>
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                                        <h3>The Liquid of Life</h3>
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                                        <div class="more-box"><a href="https://2014.igem.org/Team:Imperial/Water_Report">read more</a>
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Latest revision as of 03:55, 18 October 2014

Imperial iGEM 2014

The Project

Bacterial cellulose (BC) is a natural biomaterial that is of interest in many fields due to its high purity compared with plant-derived cellulose. We are optimising BC biosynthesis in Gluconacetobacter xylinus, transferring the system to E. coli, and functionalising the material using proteins in order to expand its properties.

BC has seen use in clothing and health foods, but we develop its application to the global issue of water purification. Rising demand for limited freshwater supplies will lead to more than half of the global population suffering severe water stress by 2030. Improved filtration techniques would help relieve this problem.

The porosity of BC and our synthetic attachment of contaminant-specific binding and catabolic proteins make for a flexible, modular water filter. Our manufactured biomaterial would augment water recycling and reclamation on local and industrial scales, helping to alleviate water stress.

Implementation

Our final idea

Water Report

The Liquid of Life

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