Team:Hong Kong HKUST/wetlab/notebook

From 2014.igem.org

(Difference between revisions)
Line 1,605: Line 1,605:
. Digest 005 Spe1 and Pst1 <br>
. Digest 005 Spe1 and Pst1 <br>
. ligate CLON 005 with BBa_B0015 <br>
. ligate CLON 005 with BBa_B0015 <br>
-
. prepare backbone : BBa_B0015 E, X /// BBa_I13401 E,X /// J23102 X, P <br>
+
. prepare backbone : BBa_B0015 E, X /// BBa_I13401 E,X /// BBa_J23102 X, P <br>
. Ligate P<sub>bad</sub> with Key1 dTT <br>
. Ligate P<sub>bad</sub> with Key1 dTT <br>
. digest P<sub>tet</sub> lock 1 with S and P <br>
. digest P<sub>tet</sub> lock 1 with S and P <br>
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. pSB1C3-BBa_J04450 for oligo ligation <br>
. pSB1C3-BBa_J04450 for oligo ligation <br>
. Gel check for PCR products (19/08) <br>
. Gel check for PCR products (19/08) <br>
-
. PCR check for J23102, LOCK 3 GFP DTT <br>
+
. PCR check for BBa_J23102, LOCK 3 GFP DTT <br>
. Restriction check for key1 DTT <br>
. Restriction check for key1 DTT <br>
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. restriction check for key 1 <br>
. restriction check for key 1 <br>
. P<sub>bad</sub> for ust key 1 <br>
. P<sub>bad</sub> for ust key 1 <br>
-
. extract ust lock 1 for j23102 <br>
+
. extract ust lock 1 for BBa_J23102 <br>
. ligate ust lock and key <br>
. ligate ust lock and key <br>
. transform ust lock and key <br>
. transform ust lock and key <br>
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. digestion of P<sub>bad</sub> MTA dTT <br>
. digestion of P<sub>bad</sub> MTA dTT <br>
. digestion of P<sub>bad</sub> dTT <br>
. digestion of P<sub>bad</sub> dTT <br>
-
. digestion of J23102 MCR GFP dTT <br>
+
. digestion of BBa_J23102 MCR GFP dTT <br>
-
. digestion of J23102 BBa_E0240(B0032 GFP dTT) <br>
+
. digestion of BBa_J23102 BBa_E0240(BBa_B0032 GFP dTT) <br>
. digestion of pSB3K3 backbone (from pSB3K3-BBa_J04450) <br>
. digestion of pSB3K3 backbone (from pSB3K3-BBa_J04450) <br>
-
. Ligation of J23102 MCR GFP dTT with P<sub>bad</sub> dTT <br>
+
. Ligation of BBa_J23102 MCR GFP dTT with P<sub>bad</sub> dTT <br>
-
. Ligation of J23102 BBa_E0240 with P<sub>bad</sub> MTA dTT <br>
+
. Ligation of BBa_J23102 BBa_E0240 with P<sub>bad</sub> MTA dTT <br>
-
. Ligation of J23102 BBa_E0240 with P<sub>bad</sub> dTT <br>
+
. Ligation of BBa_J23102 BBa_E0240 with P<sub>bad</sub> dTT <br>
. Plasmid PCR for SherLock-GFP-DTT <br>
. Plasmid PCR for SherLock-GFP-DTT <br>
. ligation of B0034-GFP-DTT <br>
. ligation of B0034-GFP-DTT <br>
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Wet lab<br>
Wet lab<br>
. Gel purification of digested insert and backbone <br>
. Gel purification of digested insert and backbone <br>
-
. Ligation of J23102 MCR GFP dTT with P<sub>bad</sub> dTT <br>
+
. Ligation of BBa_J23102 MCR GFP dTT with P<sub>bad</sub> dTT <br>
-
. Ligation of J23102 BBa_E0240 with P<sub>bad</sub> MTA dTT <br>
+
. Ligation of BBa_J23102 BBa_E0240 with P<sub>bad</sub> MTA dTT <br>
-
. digestion of J23102 BBa_E0240(B0032 GFP dTT) <br>
+
. digestion of BBa_J23102 BBa_E0240(BBa_B0032 GFP dTT) <br>
. Transformation of ligated projects mentioned above <br>
. Transformation of ligated projects mentioned above <br>
. Colony PCR for sherLock-GFP-DTT <br>
. Colony PCR for sherLock-GFP-DTT <br>
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. miniprep/ glycerol stock <br>
. miniprep/ glycerol stock <br>
. Transform P<sub>bad</sub> dTT <br>
. Transform P<sub>bad</sub> dTT <br>
-
. Transform ligated produce of J23102 MCR GFP dTT with P<sub>bad</sub> dTT <br>
+
. Transform ligated produce of BBa_J23102 MCR GFP dTT with P<sub>bad</sub> dTT <br>

Revision as of 14:54, 17 October 2014



Notebook

Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and research findings. The notebook for Project Pneuumonsensor and Project Riboregulator are displayed separately.






Pneumosensor

June 2014

Week 1
Week 2
Week 3
Week 4

July 2014

Week 1
Week 2
Week 3
Week 4
Week 5

August 2014

Week 1
Week 2
Week 3
Week 4
Week 5

September 2014

Week 1
Week 2
Week 3
Week 4
Week 5





Riboregulator

July 2014

Week 1
Week 3
Week 4
Week 5

August 2014

Week 1
Week 2
Week 3
Week 4
Week 5

September 2014

Week 1
Week 2
Week 3
Week 4
Week 5


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