Team:Hong Kong HKUST/wetlab/notebook

From 2014.igem.org

(Difference between revisions)
Line 1,653: Line 1,653:
<br> <b> July 16</b> <br>
<br> <b> July 16</b> <br>
Wet lab<br>
Wet lab<br>
-
·     <br>
+
· Gibson assembly master mix preparation<br>
-
·     <br>
+
· Preparations for Pbad re-characterization <br>
-
·     <br>
+
· Competent cells preparations for  DH5a and DH10B<br>
-
·   <br>
+
· Prepare receptor backbone for oligo anealing <br>
 +
· Innoculate succesful oligo annealing colonies<br>
 +
· Oligo annealing products C. PCR<br>
 +
 
<br> <b> July 17</b> <br>
<br> <b> July 17</b> <br>
Wet lab<br>
Wet lab<br>
-
·     <br>
+
· Gibson assembly master mix preparation<br>
-
·     <br>
+
· Pbad re-characterization <br>
-
·     <br>
+
· Miniprep CRs TAs<br>
-
·   <br>
+
· Restric check CRs,TAs<br>
 +
. Oligo annealing products C. PCR<br>
 +
. Prepare receptor backbone for oligo anealing <br>
 +
. Restrict CRs plasmids for GFP.dTT<br>
 +
. Restrict TAs plasmids for dTT<br>
 +
. Restrict GFP.dTT for CRs<br>
 +
. Restrict dTT for TAs<br>
 +
. Ligate CR.GFP.dTT<br>
 +
. Ligate TA.dTT<br>
 +
. Transform Ligated CR.GFP.dTT<br>
 +
. Transform Ligated TA.dTT<br>
 +
. Transform-Ligate Annealed oligos<br>
 +
 
<br> <b> July 18</b> <br>
<br> <b> July 18</b> <br>
Wet lab<br>
Wet lab<br>
-
·     <br>
+
· Gibson assembly for amilCP<br>
-
·     <br>
+
· Pbad re-characterization <br>
-
·     <br>
+
· Colony PCR <br>
-
·   <br>
+
· Prepare receptor backbone for oligo anealing <br>
 +
· Restrict GFP.dTT for CRs<br>
 +
· Restrict dTT for TAs<br>
 +
· M9 mininaml medium preparation<br>
 +
· Colony PCR <br>
 +
· plate streaking for pBAD-E0240<br>
 +
· inoculation of TAs and CRs<br>
 +
 
 +
<br> <b> July 19</b> <br>
 +
Wet lab<br>
 +
. Pbad re-characterization <br>
       </div>
       </div>
   </div>
   </div>
Line 1,677: Line 1,702:
<br> <b> July 21 </b> <br>
<br> <b> July 21 </b> <br>
Wet lab<br>
Wet lab<br>
-
·     <br>
+
· Gibson assembly for amilCP<br>
-
·      <br>
+
. Miniprep CRs TAs<br>
-
·      <br>
+
. Restrict check pSB1C3-TAs/CRs constructs <br>
-
·   <br>
+
. Restrict GFP.dTT for CRs<br>
 +
. Restrict Pbad for TAs<br>
 +
. Restrict B0015 EcoR1 and XbaI<br>
 +
. J04450 backbone extraction <br>
 +
. Colony PCR <br>
 +
. inoculation of TAs and CRs<br>
 +
. prepare Pbad L(+) A. concentration gradients for characterization <br>
 +
. Restrict CRs<br>
 +
. Restrict TAs-Version1<br>
 +
. Restrict TAs-Version2<br>
 +
. Ligate CR.GFP.dTT<br>
 +
. Ligate TA.dTT<br>
 +
. Transform Pbad.TA<br>
 +
. Transform CR.GFP.dTT<br>
 +
. Transform Gibson assembly <br>
 +
 
<br> <b> July 22 </b> <br>
<br> <b> July 22 </b> <br>
Wet lab<br>
Wet lab<br>
-
·     <br>
+
· Colony PCR <br>
-
·      <br>
+
. Miniprep CRs TAs <br>
-
·      <br>
+
. Restrict check pSB1C3-TAs/CRs constructs <br>
-
·   <br>
+
. Restrict B0015-Version2<br>
<br> <b> July 23</b> <br>
<br> <b> July 23</b> <br>
Wet lab<br>
Wet lab<br>
-
·     <br>
+
· Inoculation candidate colony or construct <br>
-
·      <br>
+
. Miniprep and restrict check innoculations<br>
-
·      <br>
+
. Gibson assembly check<br>
-
·   <br>
+
. Gibson PCR<br>
 +
. Teach inoue competent cells to main project<br>
 +
. Miniprep of B0015, pBAD-E0240<br>
 +
. glycerol stock (pBAD-E0240)<br>
 +
. Re-Re-ligation of oligo annealing O/N <br>
<br> <b> July 24</b> <br>
<br> <b> July 24</b> <br>
Wet lab<br>
Wet lab<br>
-
·     <br>
+
· miniprep candidate colony or construct <br>
-
·      <br>
+
. Restrict check pSB1C3-TAs/CRs constructs<br>  
-
·      <br>
+
. Re-Re-ligation trasnformation<br>
-
·   <br>
+
. Re-Re-ligation trasnformation V.X<br>
 +
. Gibson assembly check<br>
<br> <b> July 25</b> <br>
<br> <b> July 25</b> <br>
Wet lab<br>
Wet lab<br>

Revision as of 15:50, 10 October 2014




Notebook

Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and research findings. The notebook for Pneuumonsensor and Riboregulator are displayed separately.






Pneumosensor

June 2014

Week 1
Week 2
Week 3
Week 4
Week 5

July 2014

Week 1
Week 2
Week 3
Week 4
Week 5

August 2014

Week 1
Week 2
Week 3
Week 4
Week 5

September 2014

Week 1
Week 2
Week 3
Week 4
Week 5





Riboregulator

June 2014

Week 1
Week 2
Week 3
Week 4
Week 5

July 2014

Week 1
Week 2
Week 3
Week 4
Week 5

August 2014

Week 1
Week 2
Week 3
Week 4
Week 5

September 2014

Week 1
Week 2
Week 3
Week 4
Week 5

Home

Pneumosensor

Riboregulator

Human Practice

Team

WetLab

Achievement