Team:Hong Kong HKUST/wetlab/notebook

From 2014.igem.org

(Difference between revisions)
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<br><b>June 20</b> <br>
<br><b>June 20</b> <br>
· Amended primers of Pcombox sequences<br>
· Amended primers of Pcombox sequences<br>
-
· <br>
+
· Miniprep of BBa_J04450(CHL) <br>
-
· <br>
+
· Digestion of BBa_J04450(CHL)<br>
-
· <br>
+
· Gel electrophoresis of digested product<br>
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·      Primer design for PCR of comX-myc and comW-FLAG from genomic DNA of S. Pneumoniae NCTC 7465 strain<br>
·      Primer design for PCR of comX-myc and comW-FLAG from genomic DNA of S. Pneumoniae NCTC 7465 strain<br>
·      Designed plasmid construct for Pcombox<br>
·      Designed plasmid construct for Pcombox<br>
-
·      <br>
+
·      Bacterial inoculation of BBa_J04450(CHL)<br>
·      <br>
·      <br>
·      <br>
·      <br>
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·      Designed forward and reverse oligos of 67bp Pcombox<br>
·      Designed forward and reverse oligos of 67bp Pcombox<br>
·      Transformation of pSB1C3-BBa_I13504<br>
·      Transformation of pSB1C3-BBa_I13504<br>
-
·      <br>
+
·      Miniprep of BBa_J04450(CHL)<br>
-
·      <br>
+
·      Transformation of BBa_J04450(KAN)<br>
-
·      <br>
+
·      Bacterial inoculation of BBa_E0240<br>
·   <br>
·   <br>
<br><b>June 25</b> <br>
<br><b>June 25</b> <br>
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·      Inoculation of BBa_K880005<br>
·      Inoculation of BBa_K880005<br>
·      Inoculation of pSB1C3-BBa_E0240<br>
·      Inoculation of pSB1C3-BBa_E0240<br>
-
·      <br>
+
·      Miniprep of BBa_E0240<br>
-
·      <br>
+
·      Digestion of BBa_E0240<br>
-
·      <br>
+
·      Transformation of BBa_J04450(KAN)<br>
-
·      <br>
+
·      Bacterial inoculation of BBa_J04450(CHL)<br>
·      <br>
·      <br>
·      <br>
·      <br>
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·      Digestion of BBa_K880005 plasmid extraction product with SpeI and PstI, gel extraction and gel purification<br>
·      Digestion of BBa_K880005 plasmid extraction product with SpeI and PstI, gel extraction and gel purification<br>
·      Digestion of pSB1C3-BBa_E0240 at XbaI and PstI<br>
·      Digestion of pSB1C3-BBa_E0240 at XbaI and PstI<br>
-
·      <br>
+
·      Gel electrophoresis of digested BBa_E0240<br>
-
·   <br>
+
·     Gel extraction and purification of digested BBa_E0240 to obtain the insert  <br>
 +
·      Miniprep of BBa_J04450(CHL) <br>
 +
·      Digestion of BBa_J04450(CHL) <br>
 +
·      Gel electrophoresis of digested BBa_J04450(CHL)<br>
 +
·      Gel extraction and purification of digested BBa_J04450(CHL) to obtain pSB1C3<br>
 +
·      Bacterial inoculation of BBa_K880005<br>
 +
 
<br><b>June 27</b> <br>
<br><b>June 27</b> <br>
Wet lab<br>
Wet lab<br>
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·      Digestion of pSB3K3-BBa_J04450 at XbaI and PstI<br>
·      Digestion of pSB3K3-BBa_J04450 at XbaI and PstI<br>
·      Gel purification of pSB1C3-BBa_E0240<br>
·      Gel purification of pSB1C3-BBa_E0240<br>
-
·   <br>
+
·     Miniprep of BBa_K880005  <br>

Revision as of 16:54, 10 October 2014




Notebook

Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and research findings. The notebook for Pneuumonsensor and Riboregulator are displayed separately.






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June 2014

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July 2014

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September 2014

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Riboregulator

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