Team:Hong Kong HKUST/wetlab/notebook

From 2014.igem.org

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Wet lab<br>
Wet lab<br>
·      Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600<br>
·      Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600<br>
-
·      <br>
+
·      Transformation of BBa_B0034 and BBa_E0040 into DH10B<br>
·      <br>
·      <br>
·   <br>
·   <br>
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·      Found reference promoter J23101 in BBa_I20260 from part registry<br>
·      Found reference promoter J23101 in BBa_I20260 from part registry<br>
·      Looked for Pcombox gene sequence from the genomic DNA<br>
·      Looked for Pcombox gene sequence from the genomic DNA<br>
-
·      <br>
+
·      Bacterial inoculation of BBa_B0034 and BBa_E0040<br>
·   <br>
·   <br>
<br><b>June 11</b> <br>
<br><b>June 11</b> <br>
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·      Looked for Pcombox gene sequence from the genomic DNA<br>
·      Looked for Pcombox gene sequence from the genomic DNA<br>
·      Studied the Characteristic, function and mechnism of how promoters work<br>
·      Studied the Characteristic, function and mechnism of how promoters work<br>
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·      <br>
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·      Miniprep of BBa_B0034 and BBa_E0040<br>
·   <br>
·   <br>
<br><b>June 12</b> <br>
<br><b>June 12</b> <br>
Wet lab<br>
Wet lab<br>
·      Looked for Pcombox gene sequence from the genomic DNA<br>
·      Looked for Pcombox gene sequence from the genomic DNA<br>
-
·      <br>
+
·      research on gene sequence of PcomX, how to quantify p~comE, range of concentration of  p~comE to use for characterization<br>
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·      <br>
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·      explain of scar<br>
·   <br>
·   <br>
<br><b>June 13</b> <br>
<br><b>June 13</b> <br>
Wet lab<br>
Wet lab<br>
·      Looked for Pcombox gene sequence from the genomic DNA<br>
·      Looked for Pcombox gene sequence from the genomic DNA<br>
-
·      <br>
+
·      research on gene sequence of PcomX, how to quantify p~comE, range of concentration of  p~comE to use for characterization<br>
·      <br>
·      <br>
·   <br>
·   <br>
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·      Blast the gDNA of NCTC7465 strain with various strain of S. pneumoniae to look for Pcombox gene sequence<br>
·      Blast the gDNA of NCTC7465 strain with various strain of S. pneumoniae to look for Pcombox gene sequence<br>
·      Found a possible Pcombox<br>
·      Found a possible Pcombox<br>
-
·      <br>
+
·      Transformation of BBa_J04450 and BBa_I20260 into DH10B<br>
 +
·      Bacterial inoculation of BBa_E0240<br>
·   <br>
·   <br>
<br><b>June 17</b> <br>
<br><b>June 17</b> <br>
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·      Found a possible Pcombox<br>
·      Found a possible Pcombox<br>
·      Searched for other possible Pcombox sequences<br>
·      Searched for other possible Pcombox sequences<br>
 +
·      Miniprep of BBa_E0240<br>
 +
·      Bacterial inoculation of BBa_J04450(CHL) and BBa_I20260<br>
·   <br>
·   <br>
<br><b>June 18</b> <br>
<br><b>June 18</b> <br>

Revision as of 15:45, 16 October 2014



Notebook

Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and research findings. The notebook for Pneuumonsensor and Riboregulator are displayed separately.






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