Team:Hong Kong HKUST/wetlab/notebook

From 2014.igem.org

(Difference between revisions)
Line 132: Line 132:
Wet lab<br>
Wet lab<br>
·      Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600<br>
·      Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600<br>
-
· <br>
+
·     Transformation of BBa_B0034 and BBa_E0040 into DH10B<br>
·      <br>
·      <br>
·   <br>
·   <br>
Line 139: Line 139:
·      Found reference promoter J23101 in BBa_I20260 from part registry<br>
·      Found reference promoter J23101 in BBa_I20260 from part registry<br>
·      Looked for Pcombox gene sequence from the genomic DNA<br>
·      Looked for Pcombox gene sequence from the genomic DNA<br>
-
·      <br>
+
·      Bacterial inoculation of BBa_B0034 and BBa_E0040<br>
·   <br>
·   <br>
<br><b>June 11</b> <br>
<br><b>June 11</b> <br>
Line 145: Line 145:
·      Looked for Pcombox gene sequence from the genomic DNA<br>
·      Looked for Pcombox gene sequence from the genomic DNA<br>
·      Studied the Characteristic, function and mechnism of how promoters work<br>
·      Studied the Characteristic, function and mechnism of how promoters work<br>
-
·      <br>
+
·      Miniprep of BBa_B0034 and BBa_E0040<br>
·   <br>
·   <br>
<br><b>June 12</b> <br>
<br><b>June 12</b> <br>
Wet lab<br>
Wet lab<br>
·      Looked for Pcombox gene sequence from the genomic DNA<br>
·      Looked for Pcombox gene sequence from the genomic DNA<br>
-
·      <br>
+
·      Research on gene sequence of PcomX, how to quantify p~comE, range of concentration of  p~comE to use for characterization<br>
·      <br>
·      <br>
·   <br>
·   <br>
Line 156: Line 156:
Wet lab<br>
Wet lab<br>
·      Looked for Pcombox gene sequence from the genomic DNA<br>
·      Looked for Pcombox gene sequence from the genomic DNA<br>
-
·      <br>
+
·      Research on gene sequence of PcomX, how to quantify p~comE, range of concentration of  p~comE to use for characterization<br>
·      <br>
·      <br>
·   <br>
·   <br>
Line 169: Line 169:
·      Blast the gDNA of NCTC7465 strain with various strain of S. pneumoniae to look for Pcombox gene sequence<br>
·      Blast the gDNA of NCTC7465 strain with various strain of S. pneumoniae to look for Pcombox gene sequence<br>
·      Found a possible Pcombox<br>
·      Found a possible Pcombox<br>
-
·      <br>
+
·      Transformation of BBa_J04450 and BBa_I20260 into DH10B<br>
 +
·      Bacterial inoculation of BBa_E0240<br>
·   <br>
·   <br>
<br><b>June 17</b> <br>
<br><b>June 17</b> <br>
Line 176: Line 177:
·      Found a possible Pcombox<br>
·      Found a possible Pcombox<br>
·      Searched for other possible Pcombox sequences<br>
·      Searched for other possible Pcombox sequences<br>
 +
·      Miniprep of BBa_E0240<br>
 +
·      Bacterial inoculation of BBa_J04450(CHL) and BBa_I20260<br>
·   <br>
·   <br>
<br><b>June 18</b> <br>
<br><b>June 18</b> <br>
Line 181: Line 184:
·      Designed primers for the Pcombox sequences<br>
·      Designed primers for the Pcombox sequences<br>
·      Received a Pcombox sequence from Wellcome Trust Sanger Institute<br>
·      Received a Pcombox sequence from Wellcome Trust Sanger Institute<br>
-
·      <br>
+
·      Miniprep of BBa_J04450(CHL) and BBa_I20260<br>
 +
·      Bacterial inoculation of BBa_J04450(CHL) and BBa_I20260<br>
 +
·      Digestion of BBa_J04450(CHL) and BBa_I20260<br>
 +
·      Gel electrophoresis of digested products<br>
 +
 
 +
 
<br><b>June 19</b> <br>
<br><b>June 19</b> <br>
· Digestion of pSB1C3-BBa_E0240 at EcoRI and SpeI<br>
· Digestion of pSB1C3-BBa_E0240 at EcoRI and SpeI<br>
-
· <br>
+
· Miniprep of BBa_J04450(CHL) and BBa_I20260<br>
-
· <br>
+
· Digestion of BBa_J04450(CHL) <br>
-
· <br>
+
· Gel electrophoresis of digested product<br>
 +
· Bacterial inoculation of BBa_J04450(CHL) <br>
 +
 
 +
 
<br><b>June 20</b> <br>
<br><b>June 20</b> <br>
· Amended primers of Pcombox sequences<br>
· Amended primers of Pcombox sequences<br>

Revision as of 16:45, 10 October 2014




Notebook

Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and research findings. The notebook for Pneuumonsensor and Riboregulator are displayed separately.






Pneumosensor

June 2014

Week 1
Week 2
Week 3
Week 4
Week 5

July 2014

Week 1
Week 2
Week 3
Week 4
Week 5

August 2014

Week 1
Week 2
Week 3
Week 4
Week 5

September 2014

Week 1
Week 2
Week 3
Week 4
Week 5





Riboregulator

June 2014

Week 1
Week 2
Week 3
Week 4
Week 5

July 2014

Week 1
Week 2
Week 3
Week 4
Week 5

August 2014

Week 1
Week 2
Week 3
Week 4
Week 5

September 2014

Week 1
Week 2
Week 3
Week 4
Week 5

Home

Pneumosensor

Riboregulator

Human Practice

Team

WetLab

Achievement