Team:Hong Kong HKUST/riboregulator/results

From 2014.igem.org

(Difference between revisions)
(Blanked the page)
 
(12 intermediate revisions not shown)
Line 1: Line 1:
-
{{Team:Hong_Kong_HKUST/shell|
 
-
<html><head>
 
-
</head></html>
 
-
|
 
-
<html><body>
 
-
<div id="content_container">
 
-
<hr>
 
-
<div id="description_area">
 
-
<br><h2>Riboregulator Results</h2><br>
 
-
</div>
 
-
 
-
<!-- one row of content , two column-->
 
-
<div class='content_1'><h3>Overview</h3>
 
-
<table class="content_table" align= "center" valign= "top">
 
-
<tr class= "content_row">
 
-
<td class= "content_cell">
 
-
<div class= "content_area_one_row">
 
-
<p>Although there is a significant number of regulatory RNAs available in the registry, a comprehensive characterization information that the iGEM community can
 
-
use to compare and contrast different regulatory RNAs (especially CR-TA riboregulators) is missing.Therefore we wanted to  provide characterization information of
 
-
regulatory RNAs so teams and labs will be confident in using these devices. </p>
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
</div>
 
-
<!-- end of one row of content , two column-->
 
-
 
-
<!-- one row of content , two column one picture right-->
 
-
<div class='content_1'><h3>Construct</h3>
 
-
<table class="content_table" align= "center" >
 
-
<tr class= "content_row">
 
-
<td class= "content_cell">
 
-
<div class= "content_area_one_row">
 
-
<p>In order to provide reliable characterisation data which will help iGEM teams to identify which CR-TA riboregulator works best for their projects. We have built
 
-
constructs like the diagram provided below:
 
-
(provide diagram)
 
-
<p>Cis-repressing part which was generated by using the Lock and Key algorithm generator from TU Delft 2009 iGEM team and followed by de novo synthesis from oligo
 
-
annealing. After annealing, these dsDNA was directly inserted into the vector containing <a href="http://parts.igem.org/Part:BBa_I13401">BBa_I13401</a> ( GFP reporter for RHS of library test constructs). A promoter,
 
-
<a href="http://parts.igem.org/Part:BBa_J23102">BBa_J23102</a> was placed upstream by the scar formation of cis-repressing part therefore cis-repressing part can be
 
-
constitutively transcribed.Trans-activating part was produced by the same method as cis-repressing part but an inducible promoter, P<sub>bad</sub>, <a href="http://parts.igem.org/Part:BBa_I13401">BBa_I0500 </a>and double terminator,
 
-
<a href="http://parts.igem.org/Part:BBa_BBa_B0015">BBa_B0015 </a>were used to replace <a href="http://parts.igem.org/Part:BBa_I13401">BBa_J23102 </a>and
 
-
<a href="http://parts.igem.org/Part:BBa_I13401">BBa_I13401 </a>respectively. A low copy plasmid, pSB3K3 was used for GFP expression measurements. For the control of our characterisation,  construct containing only RBS,
 
-
instead of CR  and another construct missing TA were built. To obtain the fluorescence level, autofluorescence had to be subtracted so bacteria containing pSB3K3-E0240
 
-
plasmid were used. We have submitted Cis-repressing and Trans-activating parts as well as some of intermediary parts. </p>
 
-
</div>
 
-
</td>
 
-
 
-
</tr>
 
-
</table>
 
-
</div>
 
-
<!-- end of one row of content , two column-->
 
-
</div>
 
-
<br><br>
 
-
<div  id="content_container">
 
-
<div id="description_area"><div id="2">
 
-
<br><p style= "font-size: 30px; text-align:center"><i>S. pneumoniae</i> &sigma;<sup>x</sup> Promoters Module</p><br></div>
 
-
 
-
</div>
 
-
 
-
<!-- one row of content , two column one picture left-->
 
-
<div class='content_1'><h3> Results</h3>
 
-
<table class="content_table" align= "center" >
 
-
 
-
<tr class= "content_row">
 
-
<td class= "content_cell">
 
-
<div class= "content_area_one_row">
 
-
<div class="content_image">
 
-
 
-
</div>
 
-
<p>
 
-
<img style= "width:50%" src= "#"/>
 
-
<br>
 
-
The above bar graphs represents the level of fluorescence measured by ? and it was plotted as fluorescence/OD600 on y-axis. Culture containing bacteria was
 
-
incubated for X hours. (From left to right) For controls of each set of an experiment, (1) a construct without trans-activating part (2) a construct without
 
-
cis-repressing part but with a ribosomal binding site (3) a construct without both cis-repressing and trans-activating parts. For our experiment, a construct
 
-
containing both cis-repressing and trans-activating parts was used.  arabinose concentration of 0%, 1% and 2.5% were used to induce the inducible promoter, P<sub>bad</sub>
 
-
(<a href="https://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/characterization">P<sub>bad</sub> characterisation</a>). </p>
 
-
 
-
<br><br>
 
-
<img style= "width:50%" src= "#"/>
 
-
<br>
 
-
<p>Furthermore the orthogonality of five different sets of riboregulators were tested in parallel  to the repression and activation strength of riboregulators.
 
-
The increase in fluorescence level was calculated using the fluorescence level obtained by plate reader at 0% and 2.5% of arabinose concentrations.</p>
 
-
</div>
 
-
</td>
 
-
 
-
 
-
</tr>
 
-
</div>
 
-
</table>
 
-
</div>
 
-
 
-
<div class='content_1'><h3> Discussion</h3>
 
-
<table class="content_table" align= "center" >
 
-
 
-
<tr class= "content_row">
 
-
<td class= "content_cell">
 
-
 
-
<div class= "content_area_one_row">
 
-
<div class="content_image">
 
-
 
-
</div>
 
-
<p>
 
-
</p>
 
-
</div>
 
-
</td>
 
-
 
-
 
-
</tr>
 
-
</div>
 
-
</table>
 
-
</div>
 
-
 
-
<footer>
 
-
<script>
 
-
$(document).ready(function(){
 
-
 
-
//Check to see if the window is top if not then display button
 
-
$(window).scroll(function(){
 
-
if ($(this).scrollTop() > 100) {
 
-
$('.scrollToTop').fadeIn();
 
-
} else {
 
-
$('.scrollToTop').fadeOut();
 
-
}
 
-
});
 
-
 
-
//Click event to scroll to top
 
-
$('.scrollToTop').click(function(){
 
-
$('html, body').animate({scrollTop : 0},800);
 
-
return false;
 
-
});
 
-
 
-
});
 
-
</script>
 
-
<a href="#" class="scrollToTop">
 
-
<div style= "text-align:center" class="scrollToTop">
 
-
<br>
 
-
Back to top
 
-
</div>
 
-
</a>
 
-
</footer>
 
-
</body></html>
 
-
}}
 

Latest revision as of 22:58, 17 October 2014