Team:Hong Kong HKUST/riboregulator/parts

From 2014.igem.org

(Difference between revisions)
Line 255: Line 255:
  <td>MTA double terminator</td>       
  <td>MTA double terminator</td>       
  <td>Trans-Activator with terminator for MCR</td>    
  <td>Trans-Activator with terminator for MCR</td>    
-
  <td></td>
+
  <td>221</td>
</tr>
</tr>
Line 262: Line 262:
  <td>HKUST Key 1 double terminator</td>       
  <td>HKUST Key 1 double terminator</td>       
  <td>Trans-Activator with terminator for HKUST Lock 1</td>    
  <td>Trans-Activator with terminator for HKUST Lock 1</td>    
-
  <td></td>
+
  <td>219</td>
</tr>
</tr>
Line 269: Line 269:
  <td>Cis-Repressing device 1</td>       
  <td>Cis-Repressing device 1</td>       
  <td>Constitutive promoted, Cis-repressed GFP Coding device Lock 1</td>    
  <td>Constitutive promoted, Cis-repressed GFP Coding device Lock 1</td>    
-
  <td></td>
+
  <td>946</td>
</tr>
</tr>
Line 276: Line 276:
  <td>AraC Key 1</td>       
  <td>AraC Key 1</td>       
  <td>AraC inducible Trans-Activator device, Key 1</td>    
  <td>AraC inducible Trans-Activator device, Key 1</td>    
-
  <td></td>
+
  <td>1312</td>
</tr>
</tr>
Line 283: Line 283:
  <td>Cis-Repressing device 3</td>       
  <td>Cis-Repressing device 3</td>       
  <td>Constitutive promoted, Cis-repressed GFP Coding device Lock 3</td>    
  <td>Constitutive promoted, Cis-repressed GFP Coding device Lock 3</td>    
-
  <td></td>
+
  <td>948</td>
</tr>
</tr>
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  <td>AraC Key 3</td>       
  <td>AraC Key 3</td>       
  <td>AraC inducible Trans-Activator device, Key 3</td>    
  <td>AraC inducible Trans-Activator device, Key 3</td>    
-
  <td></td>
+
  <td>1312</td>
</tr>
</tr>
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  <td>Cis-Repressing device 3C</td>       
  <td>Cis-Repressing device 3C</td>       
  <td>Constitutive promoted, Cis-repressed GFP Coding device Lock 3C</td>    
  <td>Constitutive promoted, Cis-repressed GFP Coding device Lock 3C</td>    
-
  <td></td>
+
  <td>948</td>
</tr>
</tr>
Line 304: Line 304:
  <td>AraC Key 3C</td>       
  <td>AraC Key 3C</td>       
  <td>AraC inducible Trans-Activator device for, Key 3C</td>    
  <td>AraC inducible Trans-Activator device for, Key 3C</td>    
-
  <td></td>
+
  <td>1312</td>
</tr>
</tr>
Line 311: Line 311:
  <td>Cis-Repressing device MCR</td>       
  <td>Cis-Repressing device MCR</td>       
  <td>Constitutive promoted, Cis-represed GFP Coding device Lock MCR</td>    
  <td>Constitutive promoted, Cis-represed GFP Coding device Lock MCR</td>    
-
  <td></td>
+
  <td>953</td>
</tr>
</tr>
Line 318: Line 318:
  <td>AraC MTA</td>       
  <td>AraC MTA</td>       
  <td>AraC inducible Trans-Activator device, MTA</td>    
  <td>AraC inducible Trans-Activator device, MTA</td>    
-
  <td></td>
+
  <td>1302</td>
</tr>
</tr>
Line 325: Line 325:
  <td>Cis-Repressing device HKUST 1</td>       
  <td>Cis-Repressing device HKUST 1</td>       
  <td>Constitutive promoted, Cis-repressed GFP Coding device HKUST Lock 1</td>    
  <td>Constitutive promoted, Cis-repressed GFP Coding device HKUST Lock 1</td>    
-
  <td></td>
+
  <td>951</td>
</tr>
</tr>
Line 332: Line 332:
  <td>AraC HKUST Key 1</td>       
  <td>AraC HKUST Key 1</td>       
  <td>AraC inducible Trans-Activator device, HKUST Key 1</td>    
  <td>AraC inducible Trans-Activator device, HKUST Key 1</td>    
-
  <td></td>
+
  <td>1300</td>
</tr>
</tr>

Revision as of 17:26, 17 October 2014



Riboregulator Parts

Parts that we designed and submitted

Name Type Description Length
BBa_K1379015 Lock 3C RBS B0032 derivate for Lock 3C 12
BBa_K1379018 HKUST Lock 1 Cis-Repressor HKUST Lock 1 45
BBa_K1379019 HKUST Key 1 Trans-Activator for HKUST Key 1 82
BBa_K1379020 Cis-repressed GFP Lock 1 Cis-repressed GFP Generator Lock 1 903
BBa_K1379022 Cis-repressed GFP Generator Lock 3 Cis-repressed GFP Generator Lock 3 905
BBa_K1379024 Cis-repressed GFP Lock 3C Cis-repressed GFP Generator Lock 3C 905
BBa_K1379026 Cis-repressed GFP Lock MCR Cis-repressed GFP Generator MCR 910
BBa_K1379028 Cis-repressed GFP HKUST Lock 1 Cis-repressed GFP Generator HKUST Lock 1 908
BBa_K1379046 Lock 1 RBS GFP generator GFP generator using a Lock 1 modified RBS 873
BBa_K1379047 Lock 3C RBS GFP generator GFP generator using a Lock 3C modified RBS 875


Parts that we constructed and submitted

Name Type Description Length
BBa_I0500 Inducible pBad/araC promoter Inducible pBad/araC promoter 1210
BBa_J01010 Riboregulator Lock 1 Biobrick version of Isaacs' riboregulator cis repressed lock, crR12. 40
BBa_J01080 Lock3 Biobrick version of Isaacs' riboregulator cis repressed lock, crR12, and modified version of Riboregulator Lock 1. 40
BBa_J01086 Key3 Biobricked version of Isaacs' riboregulator trans activating key, taR12, and modified version of Riboregulator Key 1. 94
BBa_K175031 Lock for medium RBS (B0032) from the lock/key library TUD09 This biobrick generates a mRNA which forms a secondary structure blocking the (medium) RBS. 47
BBa_K175032 Key for lock of medium (K175031) RBS (B0032) from the lock/key library TUD09 This biobrick generates a mRNA which forms a secondary structure that opens LMR (K175031). 84
BBa_J23080 B0034 derived RBS to match spacing of J01122 RBS spacing modification to [RBS] such that the spacing between the shine-delgarno and the ORF is the same as it is in J01122. 10
BBa_K1379048 Cis repressed GFP, with Trans-activator Repression measurement device 1 with trans activator 2403


Parts that already exist in Parts Registry and are currently under characterization

Name Type Description Length
BBa_J01008 Riboregulator Lock 1 Lock 1 94
BBa_J23031 Lock 3c lock3c 42
BBa_J23008 Key 3c Encodes a short RNA sequence that "unlocks" the biobrick part J01122 (lock3) which is locked RFP. 42


Parts that we designed but did not submit

Name Type Description Length
BBa_K1379021 Key 1 Double Terminator Trans-Activator with terminator for lock 1 231
BBa_K1379023 Key 3 double terminator Trans-Activator with terminator for lock 3 231
BBa_K1379025 Key 3c double terminator Trans-Activator with terminator for lock 3C 231
BBa_K1379027 MTA double terminator Trans-Activator with terminator for MCR 221
BBa_K1379029 HKUST Key 1 double terminator Trans-Activator with terminator for HKUST Lock 1 219
BBa_K1379030 Cis-Repressing device 1 Constitutive promoted, Cis-repressed GFP Coding device Lock 1 946
BBa_K1379031 AraC Key 1 AraC inducible Trans-Activator device, Key 1 1312
BBa_K1379032 Cis-Repressing device 3 Constitutive promoted, Cis-repressed GFP Coding device Lock 3 948
BBa_K1379033 AraC Key 3 AraC inducible Trans-Activator device, Key 3 1312
BBa_K1379034 Cis-Repressing device 3C Constitutive promoted, Cis-repressed GFP Coding device Lock 3C 948
BBa_K1379035 AraC Key 3C AraC inducible Trans-Activator device for, Key 3C 1312
BBa_K1379036 Cis-Repressing device MCR Constitutive promoted, Cis-represed GFP Coding device Lock MCR 953
BBa_K1379037 AraC MTA AraC inducible Trans-Activator device, MTA 1302
BBa_K1379038 Cis-Repressing device HKUST 1 Constitutive promoted, Cis-repressed GFP Coding device HKUST Lock 1 951
BBa_K1379039 AraC HKUST Key 1 AraC inducible Trans-Activator device, HKUST Key 1 1300
BBa_K1379040 Repression device 1 Repression measurement device 1
BBa_K1379041 Repression device 3 Repression measurement device 3
BBa_K1379042 Repression device 3C Repression measurement device 3C
BBa_K1379043 Repression device Medium Repression measurement device Medium
BBa_K1379044 Cis-Repression device HKUST 1 Repression measurement device HKUST 1
BBa_K1379049 Cis repressed GFP lock 1, with Trans-activator key 1 Cis repressed GFP lock 1, with Trans-activator key 1

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