Team:Hong Kong HKUST/riboregulator/parts

From 2014.igem.org

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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379008">BBa_K1379008</a></td>
 
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  <td>Lock 1</td>     
 
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  <td>Cis-Repressor Lock 1</td>  
 
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  <td>40</td>
 
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</tr>
 
<tr>
<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379009">BBa_K1379009</a></td>  
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  <td scope="row"><a href="http://parts.igem.org/Part:"></a></td>  
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  <td>Key 1</td>       
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  <td></td>       
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  <td>Trans-Activator for Lock 1</td>    
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  <td></td>    
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  <td>94</td>
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  <td></td>
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</tr>  
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</tr>
<tr>
<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379010">BBa_K1379010</a></td>  
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  <td scope="row"><a href="http://parts.igem.org/Part:"></a></td>  
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  <td>Lock 1 RBS</td>       
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  <td></td>       
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  <td>B0034 derivate for RBS used in Lock 1</td>    
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  <td></td>    
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  <td>10</td>
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  <td></td>
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</tr>  
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</tr>
<tr>
<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379011">BBa_K1379011</a></td>  
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  <td scope="row"><a href="http://parts.igem.org/Part:"></a></td>  
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  <td>Lock 3</td>       
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  <td></td>       
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  <td>Cis-Repressor Lock 3</td>    
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  <td></td>    
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  <td>42</td>
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  <td></td>
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</tr>  
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</tr>
<tr>
<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379012">BBa_K1379012</a></td>  
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  <td scope="row"><a href="http://parts.igem.org/Part:"></a></td>  
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  <td>Key 3</td>       
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  <td></td>       
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  <td>Trans-Activator for Lock 3</td>    
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  <td></td>    
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  <td>94</td>
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  <td></td>
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</tr>  
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</tr>
<tr>
<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379013">BBa_K1379013</a></td>  
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  <td scope="row"><a href="http://parts.igem.org/Part:"></a></td>  
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  <td>[Lock 3C]</td>       
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  <td></td>       
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  <td>Cis-Repressor Lock 3C</td>    
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  <td></td>    
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  <td>42</td>
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  <td></td>
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</tr>  
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</tr>
<tr>
<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379014">BBa_K1379014</a></td>  
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  <td scope="row"><a href="http://parts.igem.org/Part:"></a></td>  
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  <td>[Key 3C]</td>       
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  <td></td>       
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  <td>Trans-Activator for Lock 3C</td>    
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  <td></td>    
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  <td>94</td>
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  <td></td>
</tr>  
</tr>  
<tr>
<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379015">BBa_K1379015</a></td>  
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379015"></a>BBa_K1379015</td>  
  <td>Lock 3C RBS</td>       
  <td>Lock 3C RBS</td>       
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  <td>B0032 derivate for RBS used in Lock 3C</td>    
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  <td>B0032 derivate for Lock 3C</td>    
  <td>12</td>
  <td>12</td>
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</tr>  
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</tr>
<tr>
<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379016">BBa_K1379016</a></td>  
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  <td scope="row"><a href="http://parts.igem.org/Part:"></a></td>  
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  <td>MCR</td>       
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  <td></td>       
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  <td>Cis-Repressor MCR</td>    
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  <td></td>    
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  <td>47</td>
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  <td></td>
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</tr>  
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</tr>
<tr>
<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379017">BBa_K1379017</a></td>  
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  <td scope="row"><a href="http://parts.igem.org/Part:"></a></td>  
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  <td>MTA</td>       
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  <td></td>       
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  <td>Trans-Activator for MCR</td>    
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  <td></td>    
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  <td>84</td>
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  <td></td>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379020">BBa_K1379020</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379020">BBa_K1379020</a></td>  
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  <td>Lock 1-repressed GFP translation unit</td>       
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  <td>Cis-repressed GFP Lock 1</td>       
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  <td>Lock 1-repressed GFP translation unit</td>    
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  <td>Cis-repressed GFP Generator Lock 1</td>    
  <td>903</td>
  <td>903</td>
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</tr>  
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</tr>
<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379022">BBa_K1379022</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379022">BBa_K1379022</a></td>  
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  <td>Lock 3-repressed GFP translation unit</td>       
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  <td>Cis-repressed GFP Generator Lock 3</td>       
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  <td>Lock 3-repressed GFP translation unit</td>    
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  <td>Cis-repressed GFP Generator Lock 3 </td>    
  <td>905</td>
  <td>905</td>
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</tr>  
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</tr>
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<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379024">BBa_K1379024</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379024">BBa_K1379024</a></td>  
  <td>Cis-repressed GFP Lock 3C</td>       
  <td>Cis-repressed GFP Lock 3C</td>       
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  <td>Lock 3C-repressed GFP translation unit</td>    
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  <tdCis-repressed GFP Generator Lock 3C</td>    
  <td>905</td>
  <td>905</td>
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  <td>910</td>
  <td>910</td>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379046">BBa_K1379046</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379046">BBa_K1379046</a></td>  
  <td>Lock 1 RBS GFP generator</td>       
  <td>Lock 1 RBS GFP generator</td>       
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  <td>GFP translational unit using RBS in Lock 1</td>    
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  <td>GFP generator using a Lock 1 modified RBS</td>    
  <td>873</td>
  <td>873</td>
</tr>  
</tr>  
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<tr>
<tr>
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379047">BBa_K1379047</a></td>  
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379047">BBa_K1379047</a></td>  
  <td>Lock 3C RBS GFP generator</td>       
  <td>Lock 3C RBS GFP generator</td>       
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  <td>GFP translational unit using RBS in Lock 3C</td>    
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  <td>GFP generator using a Lock 3C modified RBS</td>    
  <td>875</td>
  <td>875</td>
</tr>  
</tr>  
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 +
<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a></td>
 +
  <td>Inducible pBad/araC promoter</td>     
 +
  <td>Inducible pBad/araC promoter</td>  
 +
  <td>1210</td>
 +
</tr>
 +
 +
<tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J01010">BBa_J01010</a></td>
 +
  <td>Riboregulator Lock 1</td>     
 +
  <td>Biobrick version of Isaacs' riboregulator cis repressed lock, crR12.</td>  
 +
  <td>40</td>
 +
</tr>
 +
 +
<tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:"></a></td>
 +
  <td></td>     
 +
  <td></td>  
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  <td></td>
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</tr>
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<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:"></a></td>
 +
  <td></td>     
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  <td></td>  
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  <td></td>
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</tr>
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<tr>
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  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K175031">BBa_K175031</a></td>
 +
  <td>Lock for medium RBS (B0032) from the lock/key library TUD09</td>     
 +
  <td>This biobrick generates a mRNA which forms a secondary structure blocking the (medium) RBS.</td>  
 +
  <td>47</td>
 +
</tr>
 +
 +
<tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K175032">BBa_K175032</a></td>
 +
  <td>Key for lock of medium (K175031) RBS (B0032) from the lock/key library TUD09</td>     
 +
  <td>This biobrick generates a mRNA which forms a secondary structure that opens LMR (K175031).</td>  
 +
  <td>84</td>
 +
</tr>
 +
 +
<tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_J23080">BBa_J23080</a></td>
 +
  <td>B0034 derived RBS to match spacing of J01122</td>     
 +
  <td>RBS spacing modification to [RBS] such that the spacing between the shine-delgarno and the ORF is the same as it is in J01122.</td>  
 +
  <td>10</td>
 +
</tr>
 +
 +
<tr>
 +
  <td scope="row"><a href="http://parts.igem.org/Part:BBa_K1379048">BBa_K1379048</a></td>
 +
  <td>Cis repressed GFP, with Trans-activator</td>     
 +
  <td>Repression measurement device 1 with trans activator</td>  
 +
  <td>2403</td>
 +
</tr>
 +
</tbody>
</tbody>
</table>
</table>

Revision as of 15:52, 10 October 2014

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Riboregulator Parts

Name Type Description Length
BBa_K1379015 Lock 3C RBS B0032 derivate for Lock 3C 12
BBa_K1379018 HKUST Lock 1 Cis-Repressor HKUST Lock 1 45
BBa_K1379019 HKUST Key 1 Trans-Activator for HKUST Key 1 82
BBa_K1379020 Cis-repressed GFP Lock 1 Cis-repressed GFP Generator Lock 1 903
BBa_K1379022 Cis-repressed GFP Generator Lock 3 Cis-repressed GFP Generator Lock 3 905
BBa_K1379024 Cis-repressed GFP Lock 3C 905
BBa_K1379026 Cis-repressed GFP Lock MCR Cis-repressed GFP Generator MCR 910
BBa_K1379028 Cis-repressed GFP HKUST Lock 1 Cis-repressed GFP Generator HKUST Lock 1 908
BBa_K1379046 Lock 1 RBS GFP generator GFP generator using a Lock 1 modified RBS 873
BBa_K1379047 Lock 3C RBS GFP generator GFP generator using a Lock 3C modified RBS 875
BBa_I0500 Inducible pBad/araC promoter Inducible pBad/araC promoter 1210
BBa_J01010 Riboregulator Lock 1 Biobrick version of Isaacs' riboregulator cis repressed lock, crR12. 40
BBa_K175031 Lock for medium RBS (B0032) from the lock/key library TUD09 This biobrick generates a mRNA which forms a secondary structure blocking the (medium) RBS. 47
BBa_K175032 Key for lock of medium (K175031) RBS (B0032) from the lock/key library TUD09 This biobrick generates a mRNA which forms a secondary structure that opens LMR (K175031). 84
BBa_J23080 B0034 derived RBS to match spacing of J01122 RBS spacing modification to [RBS] such that the spacing between the shine-delgarno and the ORF is the same as it is in J01122. 10
BBa_K1379048 Cis repressed GFP, with Trans-activator Repression measurement device 1 with trans activator 2403

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