http://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/riboregulator/characterization&feed=atom&action=historyTeam:Hong Kong HKUST/riboregulator/characterization - Revision history2024-03-19T07:49:40ZRevision history for this page on the wikiMediaWiki 1.16.5http://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/riboregulator/characterization&diff=405138&oldid=prevHyht2011 at 03:38, 23 January 20152015-01-23T03:38:26Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br><h2>P<sub>BAD</sub> Characterization<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br><h2>P<sub>BAD</sub> Characterization<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b>(Please note that the following data has been shown to be problematic because our new data do not match our old data. We will update the following information as soon as possible)</b></h2><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b>(Please note that the following data has been shown to be problematic because our new data do not match our old data. We will update the following information as soon as possible <ins class="diffchange diffchange-inline">- HKUST iGEM 2015.23Jan2015</ins>)</b></h2><br></div></td></tr>
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</table>Hyht2011http://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/riboregulator/characterization&diff=405137&oldid=prevHyht2011 at 03:37, 23 January 20152015-01-23T03:37:47Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <br><h2>P<sub>BAD</sub> Characterization</h2><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <br><h2>P<sub>BAD</sub> Characterization<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><b>(Please note that the following data has been shown to be problematic because our new data do not match our old data. We will update the following information as soon as possible)</b></ins></h2><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><u>RPU of P<sub>BAD</sub> in different cell strains</u></b><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><u>RPU of P<sub>BAD</sub> in different cell strains</u></b><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In order to solve the discrepancy between Groningen 2011 and Cambridge 2011, we have calculated the Relative Promoter Unit of the P<sub>BAD</sub> promoter in three different cell </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In order to solve the discrepancy between Groningen 2011 and Cambridge 2011, we have calculated the Relative Promoter Unit of the P<sub>BAD</sub> promoter in three different cell </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>strains across increasing arabinose concentration. The three strains that were chosen was: DH10B, BW25113, and DH5α. Groningen 2011 used cell strain DH5α to obtain the 3-D graph </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>strains across increasing arabinose concentration. The three strains that were chosen was: DH10B, BW25113, and DH5α. Groningen 2011 used cell strain DH5α to obtain the 3-D graph </div></td></tr>
</table>Hyht2011http://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/riboregulator/characterization&diff=394499&oldid=prevJumnicole at 03:10, 18 October 20142014-10-18T03:10:30Z<p></p>
<a href="http://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/riboregulator/characterization&diff=394499&oldid=386102">Show changes</a>Jumnicolehttp://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/riboregulator/characterization&diff=386102&oldid=prevJumnicole at 02:02, 18 October 20142014-10-18T02:02:31Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <a href="#nogo"></a><img src="https://static.igem.org/mediawiki/2014/f/fe/HKUST_ribo_characterizationA.png" /><br><img style="width:<del class="diffchange diffchange-inline">70</del>%;" src="https://static.igem.org/mediawiki/2014/c/cc/HKUST_ribo_characterizationB.png"/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <a href="#nogo"></a><img src="https://static.igem.org/mediawiki/2014/f/fe/HKUST_ribo_characterizationA.png" /><br><img style="width:<ins class="diffchange diffchange-inline">90</ins>%;" src="https://static.igem.org/mediawiki/2014/c/cc/HKUST_ribo_characterizationB.png"/></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure 1. Fluorescence (F)/OD600 measurements of riboregulator pairs after arabinose induction and their corresponding controls. </h5></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure 1. Fluorescence (F)/OD600 measurements of riboregulator pairs after arabinose induction and their corresponding controls. </h5></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> margin-right: auto" src="https://static.igem.org/mediawiki/2014/0/02/FACS_hkust.png" /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> margin-right: auto" src="https://static.igem.org/mediawiki/2014/0/02/FACS_hkust.png" /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure <del class="diffchange diffchange-inline">1</del>. Forward scatter intensity (FSC) versus GFP graphs for samples with P<sub>BAD</sub> promoter regulating GFP generator. </h5></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure <ins class="diffchange diffchange-inline">2</ins>. Forward scatter intensity (FSC) versus GFP graphs for samples with P<sub>BAD</sub> promoter regulating GFP generator. </h5></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6 style= "font-size: 13px"> All samples were inoculated in M9 minimal salt medium overnight in various arabinose concentrations (%w/v). The samples were diluted around 10 fold the next day. Sample were fixed and the fluorescence was measured using flow cytometer. The graphs were plotted for the control constructs, pSB3K3-<a href="http://parts.igem.org/Part:BBa_E0240">BBa_E0240</a> (-) and pSB3K3-<a href="http://parts.igem.org/Part:BBa_I20260">BBa_I20260</a> (<a href="http://parts.igem.org/Part:BBa_J23101">BBa_J23101</a>) in the absence of arabinose. FSC versus GFP graphs for pSB3K3-<a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a>-<a href="http://parts.igem.org/Part:BBa_E0240">BBa_E0240</a>(<a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a>) in 0, 0.2 and 1.0% arabinose concentration were plotted. Each set of graphs were obtained for three different cell strains, DH10B, DH5α and BW25113. </h6> <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6 style= "font-size: 13px"> All samples were inoculated in M9 minimal salt medium overnight in various arabinose concentrations (%w/v). The samples were diluted around 10 fold the next day. Sample were fixed and the fluorescence was measured using flow cytometer. The graphs were plotted for the control constructs, pSB3K3-<a href="http://parts.igem.org/Part:BBa_E0240">BBa_E0240</a> (-) and pSB3K3-<a href="http://parts.igem.org/Part:BBa_I20260">BBa_I20260</a> (<a href="http://parts.igem.org/Part:BBa_J23101">BBa_J23101</a>) in the absence of arabinose. FSC versus GFP graphs for pSB3K3-<a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a>-<a href="http://parts.igem.org/Part:BBa_E0240">BBa_E0240</a>(<a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a>) in 0, 0.2 and 1.0% arabinose concentration were plotted. Each set of graphs were obtained for three different cell strains, DH10B, DH5α and BW25113. </h6> <br><br></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure <del class="diffchange diffchange-inline">2</del>. The percentage of cells in induced and uninduced state, and RPU across different arabinose concentration. </h5></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure <ins class="diffchange diffchange-inline">3</ins>. The percentage of cells in induced and uninduced state, and RPU across different arabinose concentration. </h5></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6 style= "font-size: 13px"> Q3 and Q4 represent the 3<sup>rd</sup> and 4<sup>th</sup> quadrants of the forward scatter versus GFP curve mentioned in Figure 2. The experimental condition was same as the procedure mentioned in the caption of the Figure 1. The left y-axis is for the percent of cells in Q3 and Q4 while the right y-axis is for RPU. Graphs depict the triplicate mean ± standard deviation. (A) Graph for pSB3K3-<a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a>-<a href="http://parts.igem.org/Part:BBa_E0240">BBa_E0240</a> in DH10B. (B) Graph for pSBK3K3-<a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a>-<a href="http://parts.igem.org/Part:BBa_E0240">BBa_E0240</a> in DH5α. (C) Graph for pSB3K3-<a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a>-<a href="http://parts.igem.org/Part:BBa_E0240">BBa_E0240</a> in BW25113. </h6> <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6 style= "font-size: 13px"> Q3 and Q4 represent the 3<sup>rd</sup> and 4<sup>th</sup> quadrants of the forward scatter versus GFP curve mentioned in Figure 2. The experimental condition was same as the procedure mentioned in the caption of the Figure 1. The left y-axis is for the percent of cells in Q3 and Q4 while the right y-axis is for RPU. Graphs depict the triplicate mean ± standard deviation. (A) Graph for pSB3K3-<a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a>-<a href="http://parts.igem.org/Part:BBa_E0240">BBa_E0240</a> in DH10B. (B) Graph for pSBK3K3-<a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a>-<a href="http://parts.igem.org/Part:BBa_E0240">BBa_E0240</a> in DH5α. (C) Graph for pSB3K3-<a href="http://parts.igem.org/Part:BBa_I0500">BBa_I0500</a>-<a href="http://parts.igem.org/Part:BBa_E0240">BBa_E0240</a> in BW25113. </h6> <br><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> margin-right: auto" src="https://static.igem.org/mediawiki/2014/a/ab/Pbad_RPU_3strains.png" /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> margin-right: auto" src="https://static.igem.org/mediawiki/2014/a/ab/Pbad_RPU_3strains.png" /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure <del class="diffchange diffchange-inline">3</del>. RPU of P<sub>BAD</sub> promoter in three different cell strains across different arabinose concentration. </h5></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure <ins class="diffchange diffchange-inline">4</ins>. RPU of P<sub>BAD</sub> promoter in three different cell strains across different arabinose concentration. </h5></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6 style= "font-size: 13px"> Relative Promoter Unit of P<sub>BAD</sub> promoter was calculated in three strains: DH10B, BW25113 and DH5alpha. Gradient arabinose concentration (% w/v) from 0% to 1.0% with 0.2% increments was used to test the variation of promoter strength (RPU) in different concentration of arabinose. Each strain of cells inoculated overnight in various arabinose concentration above. The cells were diluted around 10 fold and grown until they reached mid-log phase (OD600 0.3-0.5). Cells were fixed and fluorescence was measured using flow cytometer. The graph represent triplicate mean ±SD. </h6> <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6 style= "font-size: 13px"> Relative Promoter Unit of P<sub>BAD</sub> promoter was calculated in three strains: DH10B, BW25113 and DH5alpha. Gradient arabinose concentration (% w/v) from 0% to 1.0% with 0.2% increments was used to test the variation of promoter strength (RPU) in different concentration of arabinose. Each strain of cells inoculated overnight in various arabinose concentration above. The cells were diluted around 10 fold and grown until they reached mid-log phase (OD600 0.3-0.5). Cells were fixed and fluorescence was measured using flow cytometer. The graph represent triplicate mean ±SD. </h6> <br><br></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure <del class="diffchange diffchange-inline">4</del>. Fluorescence and OD600 measurements of DH10B and DH5α induced in different arabinose concentrations. </h5></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure <ins class="diffchange diffchange-inline">5</ins>. Fluorescence and OD600 measurements of DH10B and DH5α induced in different arabinose concentrations. </h5></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6 style= "font-size: 13px"> Triplicate of DH10B and DH5α samples were inoculated in deep well 96 well plate overnight in M9 minimal salt medium. Arabinose was added to match the final working concentration from 0 to 1.0 % (w/v) with 0.2% increments. Fluorescence and OD600 was measured every two hours for ten hours. (A) Increase of OD600 measurement for DH10B strain in different arabinose concentration. The graph represents triplicate mean ± SD (B) Increase of OD600 measurement for DH5α strain in different arabinose concentration. (C) Fluorescence VS arabinose concentration VS Time 3-D graph for DH10B. Each point represent triplicate mean. (D) Fluorescence VS arabinose concentration VS Time 3-D graph for DH5α. Each point represent triplicate mean. </h6> <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h6 style= "font-size: 13px"> Triplicate of DH10B and DH5α samples were inoculated in deep well 96 well plate overnight in M9 minimal salt medium. Arabinose was added to match the final working concentration from 0 to 1.0 % (w/v) with 0.2% increments. Fluorescence and OD600 was measured every two hours for ten hours. (A) Increase of OD600 measurement for DH10B strain in different arabinose concentration. The graph represents triplicate mean ± SD (B) Increase of OD600 measurement for DH5α strain in different arabinose concentration. (C) Fluorescence VS arabinose concentration VS Time 3-D graph for DH10B. Each point represent triplicate mean. (D) Fluorescence VS arabinose concentration VS Time 3-D graph for DH5α. Each point represent triplicate mean. </h6> <br><br></div></td></tr>
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</table>Jumnicolehttp://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/riboregulator/characterization&diff=385518&oldid=prevJumnicole at 01:57, 18 October 20142014-10-18T01:57:15Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure 1. Fluorescence (F)/OD600 measurements of riboregulator pairs after arabinose induction and their corresponding controls. </h5></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure 1. Fluorescence (F)/OD600 measurements of riboregulator pairs after arabinose induction and their corresponding controls. </h5></div></td></tr>
</table>Jumnicolehttp://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/riboregulator/characterization&diff=385256&oldid=prevJumnicole at 01:54, 18 October 20142014-10-18T01:54:37Z<p></p>
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</table>Jumnicolehttp://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/riboregulator/characterization&diff=385146&oldid=prevJumnicole at 01:53, 18 October 20142014-10-18T01:53:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure 1. Fluorescence (F)/OD600 measurements of riboregulator pairs after arabinose induction and their corresponding controls. </h5></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure 1. Fluorescence (F)/OD600 measurements of riboregulator pairs after arabinose induction and their corresponding controls. </h5></div></td></tr>
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</table>Jumnicolehttp://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/riboregulator/characterization&diff=384961&oldid=prevJumnicole at 01:51, 18 October 20142014-10-18T01:51:56Z<p></p>
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</table>Jumnicolehttp://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/riboregulator/characterization&diff=384656&oldid=prevJumnicole at 01:49, 18 October 20142014-10-18T01:49:04Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline"><--!</del><a href="#nogo"><del class="diffchange diffchange-inline">--</del>><img src="https://static.igem.org/mediawiki/2014/f/fe/HKUST_ribo_characterizationA.png" /><br><img src="https://static.igem.org/mediawiki/2014/c/cc/HKUST_ribo_characterizationB.png"/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <a href="#nogo"><ins class="diffchange diffchange-inline"></a</ins>><img src="https://static.igem.org/mediawiki/2014/f/fe/HKUST_ribo_characterizationA.png" /><br><img src="https://static.igem.org/mediawiki/2014/c/cc/HKUST_ribo_characterizationB.png"/></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure 1. Fluorescence (F)/OD600 measurements of riboregulator pairs after arabinose induction and their corresponding controls. </h5></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure 1. Fluorescence (F)/OD600 measurements of riboregulator pairs after arabinose induction and their corresponding controls. </h5></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>After repression, the system needs to be activated when taRNA is expressed. After the addition of arabinose, taRNA is expressed. Out of the three cognate riboregulator pairs that got repressed, only two showed significant increase after arabinose induction. Lock 1- Key 1 cognate paired showed around 13-fold increase for both 1% and 2.5% (%w/v) arabinose induction. Lock 3- Key 3 cognate paired showed around 1.5 and 3 fold increase for 1% and 2.5% of arabinose induction respectively. Lock 1-Key 1 and Lock 3- Key 3 behaved differently for different concentration of arabinose induction. Full induction was observed at 1% arabinose for Lock 1- Key 1 cognate pairs while full induction for Lock 3- Key 3 was observed at 2.5% arabinose. Statistically, no significant fold increase could be observed for Lock m- Key m cognate pair. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>After repression, the system needs to be activated when taRNA is expressed. After the addition of arabinose, taRNA is expressed. Out of the three cognate riboregulator pairs that </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>got repressed, only two showed significant increase after arabinose induction. Lock 1- Key 1 cognate paired showed around 13-fold increase for both 1% and 2.5% (%w/v) arabinose </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>induction. Lock 3- Key 3 cognate paired showed around 1.5 and 3 fold increase for 1% and 2.5% of arabinose induction respectively. Lock 1-Key 1 and Lock 3- Key 3 behaved differently </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>for different concentration of arabinose induction. Full induction was observed at 1% arabinose for Lock 1- Key 1 cognate pairs while full induction for Lock 3- Key 3 was observed at</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>2.5% arabinose. Statistically, no significant fold increase could be observed for Lock m- Key m cognate pair. </p<ins class="diffchange diffchange-inline">></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class='content_1'><h3>Methods</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class='content_1'><h3>Methods</h3></div></td></tr>
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</table>Jumnicolehttp://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/riboregulator/characterization&diff=384347&oldid=prevJumnicole at 01:46, 18 October 20142014-10-18T01:46:27Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 01:46, 18 October 2014</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <a href="#nogo"><img src="https://static.igem.org/mediawiki/2014/f/fe/HKUST_ribo_characterizationA.png" /><br><img src="https://static.igem.org/mediawiki/2014/c/cc/HKUST_ribo_characterizationB.png" /></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline"><--!</ins><a href="#nogo"<ins class="diffchange diffchange-inline">>--</ins>><img src="https://static.igem.org/mediawiki/2014/f/fe/HKUST_ribo_characterizationA.png" /><br><img src="https://static.igem.org/mediawiki/2014/c/cc/HKUST_ribo_characterizationB.png"/></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure 1. Fluorescence (F)/OD600 measurements of riboregulator pairs after arabinose induction and their corresponding controls. </h5></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h5 style="font-size: 13px">Figure 1. Fluorescence (F)/OD600 measurements of riboregulator pairs after arabinose induction and their corresponding controls. </h5></div></td></tr>
</table>Jumnicole