Team:Hong Kong HKUST/pneumosensor/results
From 2014.igem.org
Pneumosensor Results
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The meaning of life is a philosophical question concerning the significance of life or existence in general.
It can also be expressed in different forms, such as "Why are we here?", "What is life all about?",
and "What is the purpose of existence?" It has been the subject of much philosophical, scientific,
and theological speculation throughout history.
Overview
The activity of combox promoter is turned on by a specific sigma factor that is produced by a regulatory gene comX. The sigma factor X will bind to the combox promoter region and activate gene expression. Sigma factor X will serve as an inducer with high specificity as it binds to an area of several specific base pairs on the combox promoter. This comX and combox system could be used as a highly specific reporting system in our Streptococcus pneumonia detection platform.
However in nature, comX protein will be degraded by clpXP enzyme which exist in E.Coli and some other bacteria. Hence, to ensure the induction of combox promoter by comX, comW protein is needed as it functions to protect comX protein from being degraded by clpXP. ComW protein will be degraded instead, increasing the amount of comX protein produced.
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Contruct |
comX Generator construct (BBa_K1379006) and comW construct
Backbone pSB1C3 was used for comX generator construct and comW construct. comX gene / comW gene was fused with BBa_K880005 which contains a constitutive promoter (J23100) and strong RBS (B0032). The purpose of this strong constitutive promoter and strong RBS is to unsure the large production of comX and comW protein throughout time. Then, a double terminator (B0015) is fused with the promoter, RBS, and comX. BBa_K880005 and B0015 was obtained from 2014 iGEM distribution kit. |
ComX Tag Protein |
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Fig 1 . Here is the potato.Here is the description of the potato: it is a potato!Scientific contributions focus primarily on describing related empirical facts about the universe, exploring the context and parameters concerning the 'how' of life. Science also studies and can provide recommendations for the pursuit of well-being and a related conception of morality. |
The meaning of life is a philosophical question concerning the significance of life or existence in general. It can also be expressed in different forms, such as "Why are we here?", "What is life all about?", and "What is the purpose of existence?" It has been the subject of much philosophical, scientific, and theological speculation throughout history. There have been a large number of proposed answers to these questions from many different cultural and ideological backgrounds. The meaning of life is in the philosophical and religious conceptions of existence, social ties, consciousness, and happiness, and borders on many other issues, such as symbolic meaning, ontology, value, purpose, ethics, good and evil, free will, the existence of one or multiple gods, conceptions of God, the soul, and the afterlife. |
This will be a title
Fig 1 . Here is the potato.Here is the description of the potato: it is a potato! |
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The meaning of life is a philosophical question concerning the significance of life or existence in general. It can also be expressed in different forms, such as "Why are we here?", "What is life all about?", and "What is the purpose of existence?" It has been the subject of much philosophical, scientific, and theological speculation throughout history. |
The meaning of life is a philosophical question concerning the significance of life or existence in general. It can also be expressed in different forms, such as "Why are we here?", "What is life all about?", and "What is the purpose of existence?" It has been the subject of much philosophical, scientific, and theological speculation throughout history. |
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