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Team:Hong Kong HKUST/pneumosensor/future work - Revision history
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http://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/pneumosensor/future_work&diff=402224&oldid=prev
Jumnicole at 15:44, 14 November 2014
2014-11-14T15:44:12Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>P<sub>comCDE</sub> is a promoter induced by phosphorylated ComE, which can be replaced by a phosphorylmimetic mutant (ComE<sup><del class="diffchange diffchange-inline">D58</del></sup><del class="diffchange diffchange-inline">E</del>) in experiment. We could not </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p>P<sub>comCDE</sub> is a promoter induced by phosphorylated ComE, which can be replaced by a phosphorylmimetic mutant (ComE<sup><ins class="diffchange diffchange-inline">D58E</ins></sup>) in experiment. We could not </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> manage to verify the sequence of our ligated product, P<sub>comCDE</sub>- BBa_E0240. Therefore, in the future, we hope to continue the verification process, and complete our</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> manage to verify the sequence of our ligated product, P<sub>comCDE</sub>- BBa_E0240. Therefore, in the future, we hope to continue the verification process, and complete our</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> construct.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> construct.</p></div></td></tr>
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Jumnicole
http://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/pneumosensor/future_work&diff=345649&oldid=prev
Jumnicole at 19:36, 17 October 2014
2014-10-17T19:36:23Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <u>References</u></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <u>References</u></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> and -Resistant <i>Streptococcus pneumoniae</i> Strains&quot; 2003 </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> and -Resistant <i>Streptococcus pneumoniae</i> Strains&quot; 2003 </div></td></tr>
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Jumnicole
http://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/pneumosensor/future_work&diff=331188&oldid=prev
Jumnicole at 16:17, 17 October 2014
2014-10-17T16:17:52Z
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Jumnicole
http://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/pneumosensor/future_work&diff=321078&oldid=prev
Mfcheungaa at 13:25, 17 October 2014
2014-10-17T13:25:37Z
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Mfcheungaa
http://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/pneumosensor/future_work&diff=318851&oldid=prev
Jumnicole at 12:32, 17 October 2014
2014-10-17T12:32:34Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Our team designed but was unable to test this module due to the limitation of not being able to work directly with <i>Streptococcus </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Our team designed but was unable to test this module due to the limitation of not being able to work directly with <i>Streptococcus </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Pneumoniae</i> (Biosafety level 2) in our lab. This module proposes to kill <i>Streptococcus Pneumoniae</i> upon detection when coupled </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Pneumoniae</i> (Biosafety level 2) in our lab. This module proposes to kill <i>Streptococcus Pneumoniae</i> upon detection when coupled </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <div class='content_1'><h3>P<sub>comCDE</sub></h3></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <table class="content_table" align= "center" ></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <div class= "content_area_one_row"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <p>P<sub>comCDE</sub> is a promoter induced by phosphorylated ComE, which can be replaced by a phosphorylmimetic mutant (ComE<sup>D58</sup>E) in experiment. We could not </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> manage to verify the sequence of our ligated product, P<sub>comCDE</sub>- BBa_E0240. Therefore, in the future, we hope to continue the verification process, and complete our</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> construct.</p></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </table></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </div></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!-- one row of content , two column one picture left--></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!-- one row of content , two column one picture left--></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class='content_1'><h3>σ<sup>x</sup>, P<sub>celA</sub>, P<sub>comFA</sub> </h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class='content_1'><h3>σ<sup>x</sup>, P<sub>celA</sub>, P<sub>comFA</sub> </h3></div></td></tr>
</table>
Jumnicole
http://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/pneumosensor/future_work&diff=269596&oldid=prev
Gtong at 10:58, 16 October 2014
2014-10-16T10:58:35Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>Pneumosensor Future Work</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h2>Pneumosensor Future Work</h2></div></td></tr>
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Gtong
http://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/pneumosensor/future_work&diff=262641&oldid=prev
Jumnicole at 03:27, 16 October 2014
2014-10-16T03:27:37Z
<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p> The enzymes are tagged with osmY (<del class="diffchange diffchange-inline">Washington 2012</del><a href="http://parts.igem.org/Part:BBa_K892008?title=Part:BBa_K892008"> BBa_K892008</a>) via a linker to be exported out of <i>Escherichia coli</i>. Both enzymes have </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p> The enzymes are tagged with osmY (<a href="http://parts.igem.org/Part:BBa_K892008?title=Part:BBa_K892008">BBa_K892008</a><ins class="diffchange diffchange-inline">, Washington 2012</ins>) via a linker to be exported out of <i>Escherichia coli</i>. Both enzymes have </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> very different N-terminal catalytic sites and share a similar C-terminal cell wall attachment site, which binds to choline in both cases. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> very different N-terminal catalytic sites and share a similar C-terminal cell wall attachment site, which binds to choline in both cases. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Cleavage with either of these enzymes results in a weakening in the cell wall, which leads to the externalization of the cytoplasmic </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Cleavage with either of these enzymes results in a weakening in the cell wall, which leads to the externalization of the cytoplasmic </div></td></tr>
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Jumnicole
http://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/pneumosensor/future_work&diff=262625&oldid=prev
Jumnicole at 03:27, 16 October 2014
2014-10-16T03:27:03Z
<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p> The enzymes are tagged with osmY (Washington 2012 <a href="http://parts.igem.org/Part:BBa_K892008?title=Part:BBa_K892008"BBa_K892008</a>) via a linker to be exported out of <i>Escherichia coli</i>. Both enzymes have </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p> The enzymes are tagged with osmY (Washington 2012<a href="http://parts.igem.org/Part:BBa_K892008?title=Part:BBa_K892008"<ins class="diffchange diffchange-inline">> </ins>BBa_K892008</a>) via a linker to be exported out of <i>Escherichia coli</i>. Both enzymes have </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> very different N-terminal catalytic sites and share a similar C-terminal cell wall attachment site, which binds to choline in both cases. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> very different N-terminal catalytic sites and share a similar C-terminal cell wall attachment site, which binds to choline in both cases. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Cleavage with either of these enzymes results in a weakening in the cell wall, which leads to the externalization of the cytoplasmic </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Cleavage with either of these enzymes results in a weakening in the cell wall, which leads to the externalization of the cytoplasmic </div></td></tr>
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Jumnicole
http://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/pneumosensor/future_work&diff=262616&oldid=prev
Jumnicole at 03:26, 16 October 2014
2014-10-16T03:26:24Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Our team designed but was unable to test this module due to the limitation of not being able to work directly with <i>Streptococcus </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Our team designed but was unable to test this module due to the limitation of not being able to work directly with <i>Streptococcus </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Pneumoniae</i> (Biosafety level 2) in our lab. This module proposes to kill <i>Streptococcus Pneumoniae</i> upon detection when coupled </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Pneumoniae</i> (Biosafety level 2) in our lab. This module proposes to kill <i>Streptococcus Pneumoniae</i> upon detection when coupled </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> with the detection and <del class="diffchange diffchange-inline">regulation </del>modules by releasing specific bacteriophage lytic enzymes, Cpl-1 and Pal.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> with the detection and <ins class="diffchange diffchange-inline"> </ins>modules by releasing specific bacteriophage lytic enzymes, Cpl-1 and Pal.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p> The enzymes are tagged with osmY (Washington 2012) via a linker to be exported out of <i>Escherichia coli</i>. Both enzymes have </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p> The enzymes are tagged with osmY (Washington 2012 <ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:BBa_K892008?title=Part:BBa_K892008"BBa_K892008</a></ins>) via a linker to be exported out of <i>Escherichia coli</i>. Both enzymes have </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> very different N-terminal catalytic sites and share a similar C-terminal cell wall attachment site, which binds to choline in both cases. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> very different N-terminal catalytic sites and share a similar C-terminal cell wall attachment site, which binds to choline in both cases. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Cleavage with either of these enzymes results in a weakening in the cell wall, which leads to the externalization of the cytoplasmic </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Cleavage with either of these enzymes results in a weakening in the cell wall, which leads to the externalization of the cytoplasmic </div></td></tr>
</table>
Jumnicole
http://2014.igem.org/wiki/index.php?title=Team:Hong_Kong_HKUST/pneumosensor/future_work&diff=261290&oldid=prev
Gtong at 02:12, 16 October 2014
2014-10-16T02:12:41Z
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Gtong